FastQCFastQC Report
Wed 25 May 2016
SRR1780434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64965
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT31094.785653813591934No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT30714.727160778880936No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC11111.7101516201031324No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC5270.8112060340183176No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG3450.5310551835603787No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2450.37712614484722545No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1770.27245439852228126No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT1760.2709151081351497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1680.2586007850380974No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1470.22627568690833524No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC1170.18009697529438928No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT930.1431540060032325No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG930.1431540060032325No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT930.1431540060032325No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT850.13083968290618025No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC820.12622181174478567No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT720.11082890787347034No Hit
TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC720.11082890787347034No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG690.10621103671207574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.002215439570.056
TGAAGGT150.002215439570.044
TGGCGTG150.002215439570.039
TAGCGGA150.002215439570.050
TCTAGCA207.8725214E-570.03
GACTTTA150.002215439570.034
GATGATT150.002215439570.055
ATTGGCG150.002215439570.037
GTGAAGG150.002215439570.043
GGTAGCG150.002215439570.048
TTTACAC1600.063.43752
CTTTACA1600.063.43751
TTTATAC401.0462827E-861.253
CTATACA950.058.9473651
ATCAGGA252.3742214E-456.00000428
TGCCCAG200.006922690752.533
TTCAGCC200.006922690752.560
GGCGTGA200.006922690752.540
CCTCGCT200.006922690752.512
TGGCCAC200.006922690752.544
Produced by FastQC (version 0.11.5)