FastQCFastQC Report
Wed 25 May 2016
SRR1780433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334379
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCTC207.808147E-444.04601314
TCGAAAT207.837013E-444.01302736
ATTCGTT207.883375E-443.9603644
GTCGAAA250.002346951335.21042335
GAGTCGA353.229949E-431.40025533
CGGAATC454.0113926E-529.3860320
GCGGAAT454.0155006E-529.38162419
GGACGTG300.00572245229.3508124
TTCGGTG300.005764480729.30690641
GATCTTG300.005764480729.30690641
TTTACCG300.00576869729.3025235
CGAAACT407.0400076E-427.4875625
CTATACA1150.026.8146381
TTCGGTC508.3663865E-526.38016128
TGCGAGG801.3153294E-724.7277032
TTAAAGC450.001391260324.4590124
TTCGTAG450.001393691424.45168335
AAACTCG450.001402227724.42607527
TAATACG450.001404674524.418774
CCTGTTG651.8209614E-523.67096341
Produced by FastQC (version 0.11.5)