FastQCFastQC Report
Wed 25 May 2016
SRR1780433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334379
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT206346.17084206843133No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT157864.720990253574537Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC50341.5054773176545178No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC26880.8038782339800047RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG24530.733598700875354No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT14300.4276584354878745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC13500.40373348804799347No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC9620.28769749296457015No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG8310.24852039153176486No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5550.16597932286417508No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC4630.13846563330831183No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC4340.12979283986135493No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3620.10826038716546195RNA PCR Primer, Index 47 (95% over 22bp)
CTTATTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3350.10018571740450206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT16900.042.56688744
GCCGTCT18400.038.8576443
ATTACAC404.1089152E-738.504714
CTATACA3650.035.5872881
TTAATAC1000.035.2043082
ATGTCGA250.002350681235.1990412
CCCCGTG250.002350681235.1990437
TTGCGAT250.002350681235.1990416
CTACATG250.002350681235.1990437
CTTTACA6300.034.9458161
TTACACA6350.034.649913
TTTACAC6600.033.3374142
GTACAAG353.1993666E-431.4512311
TGAGCGT353.2134363E-431.4277159
CTTATAA502.3415068E-630.8222081
GGATCCA502.355213E-630.79916216
TTATACA31850.030.258022
CTTATAC32300.030.0588111
CTTAATA1200.029.3544851
TACCATT300.00573571529.3369242
Produced by FastQC (version 0.11.5)