FastQCFastQC Report
Wed 25 May 2016
SRR1780432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACA450.00418785531.1097565
CCGACGA808.173028E-526.24885770
TGCCGAC851.234202E-424.70480768
GCCGACG851.234202E-424.70480769
CTGCCGA901.8190859E-423.33231767
GCTGCCG952.623757E-422.10430166
ACGCTGC1205.2923224E-520.41577764
GACGCTG1205.2923224E-520.41577763
TGACGCT1257.24622E-519.59914862
TCTGACG1401.728882E-417.49923960
CGCTGCC1200.001266640617.49923765
CTGACGC1452.2611179E-416.89581561
ATCTGAC1656.0507475E-414.84783859
CATCTGA1707.588747E-414.41113858
ACATCTG1951.2853261E-414.35834957
CACATCT2050.003112563411.95069956
TACACAT2200.005277295611.13587954
GTCTCTT2250.006239345310.88841446
TTATACA2300.00734769610.651710551
ACACATC2350.00862029410.42507755
Produced by FastQC (version 0.11.5)