##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16443 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.14936447120355 31.0 21.0 34.0 12.0 34.0 2 26.74092318919905 32.0 21.0 34.0 11.0 34.0 3 27.04232804232804 32.0 21.0 34.0 11.0 34.0 4 27.351395730706077 33.0 23.0 34.0 11.0 34.0 5 27.38800705467372 33.0 23.0 34.0 11.0 34.0 6 28.728638326339475 34.0 21.0 38.0 11.0 38.0 7 28.641975308641975 34.0 21.0 37.0 11.0 38.0 8 28.442255062944717 34.0 21.0 37.0 11.0 38.0 9 28.73040199476981 34.0 21.0 37.0 12.0 38.0 10-11 28.755397433558354 34.0 21.0 38.0 11.0 38.0 12-13 28.957185428449797 34.0 21.0 38.0 11.0 38.0 14-15 28.629386365018547 34.0 21.0 38.0 11.0 38.0 16-17 28.325944170771756 34.0 21.0 38.0 11.0 38.0 18-19 28.384966247035212 34.0 21.0 38.0 11.0 38.0 20-21 28.444322812138903 34.0 21.0 38.0 11.0 38.0 22-23 28.203703703703702 34.0 21.0 38.0 11.0 38.0 24-25 28.53509092014839 34.0 21.0 38.0 11.0 38.0 26-27 28.52186340692088 34.0 21.0 38.0 11.0 38.0 28-29 28.488809827890286 34.0 21.0 38.0 11.0 38.0 30-31 28.3607310101563 34.0 16.0 38.0 11.0 38.0 32-33 28.304415252691115 34.0 21.0 38.0 11.0 38.0 34-35 28.453232378519736 34.0 21.0 38.0 11.0 38.0 36-37 28.218816517667094 34.0 21.0 38.0 11.0 38.0 38-39 28.572766526789515 34.0 21.0 38.0 11.0 38.0 40-41 28.23034117861704 34.0 18.5 38.0 11.0 38.0 42-43 28.476737821565408 34.0 21.0 38.0 11.0 38.0 44-45 27.56355287964483 34.0 15.0 37.0 11.0 38.0 46-47 28.230918932068356 34.0 21.0 37.5 11.0 38.0 48-49 28.301374445052605 34.0 21.0 37.0 11.0 38.0 50-51 28.098430943258528 34.0 21.0 37.0 11.0 38.0 52-53 28.041871921182267 34.0 21.0 37.0 11.0 38.0 54-55 27.94274159216688 34.0 21.0 37.0 11.0 38.0 56-57 27.748129903302317 33.0 16.0 37.0 11.0 38.0 58-59 27.63811348294107 33.0 15.0 37.0 11.0 38.0 60-61 27.78036246427051 33.0 19.0 37.0 11.0 38.0 62-63 27.5944170771757 33.0 15.0 37.0 11.0 38.0 64-65 27.69722678343368 33.0 19.0 37.0 11.0 38.0 66-67 27.78562306148513 33.0 19.0 37.0 11.0 38.0 68-69 27.152678951529527 31.5 15.0 37.0 11.0 38.0 70-71 27.087119138843278 32.0 11.0 37.0 11.0 38.0 72-73 27.10609377850757 32.0 15.0 37.0 11.0 38.0 74-75 26.593504834884143 31.0 11.0 37.0 11.0 38.0 76 25.652739767682295 28.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.2484418202290506 1101 2 -1.7609842977578367 1101 3 0.04273987466951468 1101 4 -2.8181213189323273 1101 5 -1.0481566841682763 1101 6 -0.6684101969639329 1101 7 -0.060760034447245204 1101 8 -0.07139675642378052 1101 9 -0.3067275223110748 1101 10-11 -0.4760546258135747 1101 12-13 -1.297389101295284 1101 14-15 -0.03789063575168683 1101 16-17 -1.5564815734927642 1101 18-19 -0.2607918913570515 1101 20-21 -0.08160010675542395 1101 22-23 -0.17529820626281634 1101 24-25 0.020985911789232148 1101 26-27 -1.6099602882792254 1101 28-29 -0.6161480961692263 1101 30-31 -1.0247076381836244 1101 32-33 0.6813591964254542 1101 34-35 -0.699788639069002 1101 36-37 -0.22769129775698715 1101 38-39 -1.3798734997157425 1101 40-41 -0.1761135389979387 1101 42-43 -1.3488359960211262 1101 44-45 1.0510798167924769 1101 46-47 -0.43582990447550785 1101 48-49 -0.894037865251974 1101 50-51 -0.010971186642279918 1101 52-53 0.024617715126449724 1101 54-55 -1.8412102459443958 1101 56-57 -0.0029640618503563587 1101 58-59 1.02946853288584 1101 60-61 -0.4141425826642333 1101 62-63 -0.6327106066205346 1101 64-65 0.048804609246456465 1101 66-67 1.3648801805636843 1101 68-69 -0.8848347101984402 1101 70-71 -0.2033911940717772 1101 72-73 0.3975169922488 1101 74-75 0.12132380324320025 1101 76 -2.2869790452393026 1102 1 -0.5633541009308054 1102 2 -3.213615876705205 1102 3 -1.4511197744532929 1102 4 -1.6330335996340821 1102 5 -1.720086508729679 1102 6 -0.5701645829288466 1102 7 -0.29672494672794514 1102 8 -1.8468353529150079 1102 9 -2.154095943363707 1102 10-11 -1.887019538094279 1102 12-13 -4.035108399540899 1102 14-15 -2.712452039260455 1102 16-17 -3.1652535033173272 1102 18-19 -3.4103532948658213 1102 20-21 -2.0114246681589343 1102 22-23 -1.9529297852101806 1102 24-25 -2.429891281193221 1102 26-27 -5.623118183016068 1102 28-29 -3.116586692660455 1102 30-31 -1.8133041294116943 1102 32-33 -6.823903961469281 1102 34-35 -2.6528588145075993 1102 36-37 -2.6956737538973385 1102 38-39 -2.2974173593648715 1102 40-41 -2.3221661705768852 1102 42-43 -1.3242745925123494 1102 44-45 -6.411200884961911 1102 46-47 -3.1994263957035756 1102 48-49 -2.246230847708116 1102 50-51 -6.237286976115964 1102 52-53 -3.6920489515402153 1102 54-55 -2.21357866699703 1102 56-57 -3.8349816057100057 1102 58-59 -2.55693497588609 1102 60-61 -3.225107494944936 1102 62-63 -4.108149203111758 1102 64-65 -2.710405917069334 1102 66-67 -2.819768942243332 1102 68-69 -6.387466289145813 1102 70-71 -3.810847334422654 1102 72-73 -2.5818689726634823 1102 74-75 -5.251921810791888 1102 76 -2.7457509750638636 1103 1 -0.8800207675974718 1103 2 -0.6802825433718738 1103 3 -0.7811197744532912 1103 4 -0.5430335996340823 1103 5 -1.473419842063013 1103 6 0.16316875040448764 1103 7 -0.2733916133946117 1103 8 1.75316464708499 1103 9 0.37257072330296026 1103 10-11 0.5113137952390545 1103 12-13 -2.6384417328742344 1103 14-15 -0.715785372593789 1103 16-17 -1.0385868366506585 1103 18-19 -0.9053532948658187 1103 20-21 -1.7114246681589336 1103 22-23 -1.8679297852101833 1103 24-25 -0.7765579478598887 1103 26-27 -1.5397848496827322 1103 28-29 0.5884133073395432 1103 30-31 -1.4699707960783606 1103 32-33 -3.3439039614692803 1103 34-35 -1.5611921478409307 1103 36-37 -2.1006737538973397 1103 38-39 -0.7574173593648652 1103 40-41 0.4028338294231162 1103 42-43 -0.6042745925123505 1103 44-45 -3.8878675516285774 1103 46-47 -0.8077597290369098 1103 48-49 -0.704564181041448 1103 50-51 -0.3972869761159643 1103 52-53 -1.742048951540216 1103 54-55 -0.1819120003303638 1103 56-57 0.31001839428999034 1103 58-59 -0.9319349758860902 1103 60-61 0.2532258383883992 1103 62-63 -1.8081492031117605 1103 64-65 -0.26207258373599984 1103 66-67 -0.6881022755766679 1103 68-69 -3.0907996224791425 1103 70-71 -1.1925140010893216 1103 72-73 -0.6902023059968165 1103 74-75 -1.6769218107918853 1103 76 -2.0757509750638654 1104 1 -1.3898246891660975 1104 2 -0.04890999435226462 1104 3 -0.5648452646493709 1104 4 1.0650056160521935 1104 5 1.0132468246036552 1104 6 -0.9387920339092375 1104 7 0.5581770140563691 1104 8 -0.036051039189519685 1104 9 -0.6168410414029246 1104 10-11 -0.5948626753491801 1104 12-13 -0.4439319289526651 1104 14-15 -0.6957853725937895 1104 16-17 -0.5873123268467388 1104 18-19 0.22837219533025888 1104 20-21 0.13073219458616592 1104 22-23 0.5318741363584429 1104 24-25 0.4985400913557996 1104 26-27 0.947960248356484 1104 28-29 -1.3700180652094787 1104 30-31 -1.1446766784313027 1104 32-33 -1.6837078830379077 1104 34-35 -1.4597215596056365 1104 36-37 0.7508948735536407 1104 38-39 -1.0910448103452595 1104 40-41 -0.06285244508669052 1104 42-43 0.7257254074876478 1104 44-45 -3.101396963393281 1104 46-47 -1.4013871800173021 1104 48-49 -0.08005437711987895 1104 50-51 -0.326502662390471 1104 52-53 -0.8332254221284501 1104 54-55 -0.11701003954605227 1104 56-57 -0.5452757233570651 1104 58-59 -1.3505624268664818 1104 60-61 0.8175395638785936 1104 62-63 -0.9772668501705866 1104 64-65 0.3253783966561592 1104 66-67 0.7429761557958834 1104 68-69 -2.7536427597340456 1104 70-71 0.39503501851852363 1104 72-73 -0.7710846589379905 1104 74-75 -1.120549261772279 1104 76 -2.4869274456520998 1105 1 -1.185185086846296 1105 2 -1.2220665809305586 1105 3 -0.36496953971150603 1105 4 -0.7595594212303247 1105 5 0.04845809220928743 1105 6 -0.7457514373889396 1105 7 -0.04766391386409552 1105 8 0.26490173628686975 1105 9 0.2435566387959156 1105 10-11 -0.6624890216623527 1105 12-13 -1.8569393854563963 1105 14-15 -0.9000107247064655 1105 16-17 -0.9455351934581699 1105 18-19 -0.08065845918507009 1105 20-21 -1.3330209122903867 1105 22-23 -1.0424837758205143 1105 24-25 -0.7125203891744434 1105 26-27 -1.2909585586029202 1105 28-29 -2.8878308241158557 1105 30-31 0.024958781386427376 1105 32-33 -0.8417443370561344 1105 34-35 -1.7893611619254415 1105 36-37 -1.5380446459161163 1105 38-39 -1.6532859039658092 1105 40-41 -1.0854290813750112 1105 42-43 -0.827091493920804 1105 44-45 0.3172028709066339 1105 46-47 0.2194703179114441 1105 48-49 -0.5177097209475505 1105 50-51 -0.370620309449297 1105 52-53 -0.6631757121035946 1105 54-55 -0.6101749111284889 1105 56-57 -1.274770338104375 1105 58-59 -0.625831689501112 1105 60-61 -0.28602298790267966 1105 62-63 -0.9137830059286607 1105 64-65 -0.278598405332243 1105 66-67 -0.5698863131353527 1105 68-69 0.6822520207133458 1105 70-71 0.8423216796618505 1105 72-73 -0.8944276581094925 1105 74-75 -0.12997345398437687 1105 76 -2.147863651120204 1106 1 0.9963233184240359 1106 2 1.1218679942625336 1106 3 -0.16778644111995789 1106 4 0.35675134660247565 1106 5 0.22292424395849508 1106 6 1.1513407934152404 1106 7 1.1876836554225925 1106 8 1.694024862138754 1106 9 0.7190223362061836 1106 10-11 0.3987331500777671 1106 12-13 0.5423109552978076 1106 14-15 0.6187307564384668 1106 16-17 0.87829488377945 1106 18-19 -0.04771888626367016 1106 20-21 -0.06518810901915018 1106 22-23 1.1618551610263737 1106 24-25 1.0303237725702203 1106 26-27 1.6483871933280199 1106 28-29 1.5578756729309404 1106 30-31 0.5958356555345432 1106 32-33 1.7427627051973857 1106 34-35 1.4646143037719703 1106 36-37 0.4045950633069637 1106 38-39 0.7773138234308306 1106 40-41 0.3033714638317164 1106 42-43 1.016047988132808 1106 44-45 0.29471309353271025 1106 46-47 0.8460037118233039 1106 48-49 0.19651108777575743 1106 50-51 1.0971216260345749 1106 52-53 -0.13398443541118255 1106 54-55 0.9157224082717867 1106 56-57 0.23840549106418507 1106 58-59 1.7395704004579962 1106 60-61 1.2482796018292568 1106 62-63 1.3531411194688872 1106 64-65 1.5312607495973332 1106 66-67 1.1127579394770954 1106 68-69 1.5141466140799977 1106 70-71 1.652862342996702 1106 72-73 0.5839912423902796 1106 74-75 0.34458356555219893 1106 76 0.22037805719419978 1107 1 -0.02873871631542002 1107 2 -0.5213081843975154 1107 3 -0.3677864411199572 1107 4 1.0118381952377113 1107 5 0.8363237476805772 1107 6 -0.14965176241602407 1107 7 -0.08775058775358602 1107 8 -0.7493994554791108 1107 9 0.1510322617644988 1107 10-11 -0.6056092816840213 1107 12-13 -0.9928007072332079 1107 14-15 -1.2573238341322508 1107 16-17 -0.18833042639424846 1107 18-19 0.042851833339305756 1107 20-21 1.4219086651744028 1107 22-23 -0.3176733749537739 1107 24-25 -1.524763076065014 1107 26-27 -0.6218361317340175 1107 28-29 -0.7582533593271243 1107 30-31 -0.3068938730014352 1107 32-33 1.0658396282743112 1107 34-35 0.06188477523598834 1107 36-37 -0.4950327282563123 1107 38-39 -0.1384430003905095 1107 40-41 0.8156543422436258 1107 42-43 -0.6396592078969654 1107 44-45 -0.11248293624396055 1107 46-47 -0.3257084469856295 1107 48-49 -0.8943077707850371 1107 50-51 -0.6552356940646824 1107 52-53 0.21948950999824746 1107 54-55 -0.19370687212523663 1107 56-57 0.9054030096746075 1107 58-59 -2.0960375399886537 1107 60-61 -0.9693382641757076 1107 62-63 0.3918507968882423 1107 64-65 0.023568441905023718 1107 66-67 0.37702592955153946 1107 68-69 1.508687557008038 1107 70-71 -0.7935396421149612 1107 72-73 -0.6748176906122012 1107 74-75 -1.1794859133559896 1107 76 -1.826520205833095 1108 1 -1.3800207675974718 1108 2 -1.6469492100385388 1108 3 -0.22839250172602021 1108 4 -0.9800032966037797 1108 5 -0.6534198420630126 1108 6 1.5540778413135783 1108 7 -0.7497552497582483 1108 8 1.3895282834486267 1108 9 -2.163186852454615 1108 10-11 -0.9453528714276089 1108 12-13 -0.5972296116621116 1108 14-15 -0.24123991804833622 1108 16-17 -0.5046474427112635 1108 18-19 0.6191921596796348 1108 20-21 0.5037268469925813 1108 22-23 -0.7809600882404837 1108 24-25 -1.1480730993750399 1108 26-27 -1.5397848496827358 1108 28-29 -0.4658291169028814 1108 30-31 0.9306352645277016 1108 32-33 0.8260960385307214 1108 34-35 0.7994139127651287 1108 36-37 -0.4509767842003676 1108 38-39 0.23894627699876736 1108 40-41 -0.12746920087991498 1108 42-43 -0.17578974402750092 1108 44-45 -1.6415039152649413 1108 46-47 0.09527057399339256 1108 48-49 0.8575570310797644 1108 50-51 0.4475615087325231 1108 52-53 0.8124965030052387 1108 54-55 -0.8037301821485485 1108 56-57 1.508200212471813 1108 58-59 -2.6304198243709394 1108 60-61 -0.02738022221766201 1108 62-63 -1.1869370818996394 1108 64-65 -0.8475271291905457 1108 66-67 -0.6614356089100006 1108 68-69 0.736776135096612 1108 70-71 1.0641526655773461 1108 72-73 -0.6674750332695432 1108 74-75 1.2321690982990212 1108 76 0.5981884188755302 1109 1 -3.260229100930804 1109 2 -2.1104908767052066 1109 3 -1.8396614411199579 1109 4 -0.34032526630074855 1109 5 -1.0492531753963448 1109 6 -1.1878729162621795 1109 7 -2.4988082800612794 1109 8 -1.9864186862483422 1109 9 -1.3061792766970406 1109 10-11 -2.0911862047609446 1109 12-13 -0.4241708995409006 1109 14-15 -1.8051603725937895 1109 16-17 -1.6980660033173258 1109 18-19 -2.160874128199154 1109 20-21 -2.3109038348255986 1109 22-23 -2.5430339518768506 1109 24-25 -0.9913496145265555 1109 26-27 -1.9642640163494 1109 28-29 -2.1691908593271236 1109 30-31 -0.8565332960783607 1109 32-33 -2.0150497948026143 1109 34-35 -3.7221296478409336 1109 36-37 -2.8998404205640043 1109 38-39 -2.9885631926982015 1109 40-41 -1.5086245039102195 1109 42-43 -3.071149592512352 1109 44-45 -2.262242551628578 1109 46-47 -1.6895305623702441 1109 48-49 -1.8806058477081145 1109 50-51 -2.698745309449297 1109 52-53 -2.4545489515402146 1109 54-55 -1.8693078336636972 1109 56-57 -1.583419105710007 1109 58-59 -2.0605808092194238 1109 60-61 -0.8079199949449354 1109 62-63 -2.2065867031117605 1109 64-65 -1.8203017504026668 1109 66-67 -2.3801856089100006 1109 68-69 -1.77340378914581 1109 70-71 -2.795222334422654 1109 72-73 -2.2292648059968165 1109 74-75 -1.994630144125221 1109 76 -1.8738759750638643 1110 1 -0.23258487016157403 1110 2 -0.14053895362828328 1110 3 -1.7254787488122645 1110 4 -2.3150848816853653 1110 5 -2.025214713857885 1110 6 -2.511190223954486 1110 7 -0.2800582800612794 1110 8 -1.2147840708637254 1110 9 -2.779736969004734 1110 10-11 -3.0286862047609446 1110 12-13 -1.452416091848594 1110 14-15 -1.1188622956707128 1110 16-17 -3.180638118701939 1110 18-19 -1.9859943205068475 1110 20-21 -1.9569374886717519 1110 22-23 -1.0965195287999272 1110 24-25 -1.470916922218862 1110 26-27 -1.9660669009647869 1110 28-29 -2.8909456670194302 1110 30-31 -1.8799707960783607 1110 32-33 -1.6514680640333808 1110 34-35 -0.9631152247640138 1110 36-37 -2.8373404205640043 1110 38-39 -1.7692122311597416 1110 40-41 -1.872807196217913 1110 42-43 -2.880043823281582 1110 44-45 -2.0470983208593445 1110 46-47 -0.18532383160101418 1110 48-49 -1.7808462323235013 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3.156260749597333 2116 66-67 2.813564391090001 2116 68-69 3.68753371085419 2116 70-71 -1.1608473344226553 2116 72-73 2.209797694003182 2116 74-75 1.614744855874779 2116 76 4.504249024936136 2117 1 1.139587075539783 2117 2 0.5393252997653804 2117 3 1.7145665000565131 2117 4 1.0208879689933674 2117 5 0.10148211872130375 2117 6 0.1788550249142915 2117 7 0.04347113170342709 2117 8 2.772772490222245 2117 9 3.4713942527147275 2117 10-11 1.4713137952390554 2117 12-13 1.1148916004590994 2117 14-15 1.8042146274062105 2117 16-17 2.3759229672709097 2117 18-19 0.8166074894479038 2117 20-21 2.0866145475273434 2117 22-23 0.6054035481231494 2117 24-25 2.5426577384146185 2117 26-27 0.32296024835648396 2117 28-29 1.4240995818493474 2117 30-31 3.3700292039216393 2117 32-33 1.2721744699032698 2117 34-35 0.7093960874531824 2117 36-37 1.0597184029654088 2117 38-39 0.44571989553709557 2117 40-41 0.893029907854487 2117 42-43 2.4169018780758833 2117 44-45 0.825073624842009 2117 46-47 1.4589069376297559 2117 48-49 2.2287691522918855 2117 50-51 0.09996792584481895 2117 52-53 3.0050098719891984 2117 54-55 1.2800487839833607 2117 56-57 0.7782536884076414 2117 58-59 2.1935552201923407 2117 60-61 0.5528336815256516 2117 62-63 1.9565566792411815 2117 64-65 0.9430254554796882 2117 66-67 0.16209380285470587 2117 68-69 1.2684160637953639 2117 70-71 2.431799724400875 2117 72-73 3.2215623998855385 2117 74-75 1.460333091168895 2117 76 -0.6192803868285708 2118 1 0.1866458990691946 2118 2 1.9197174566281276 2118 3 1.4322135588800435 2118 4 3.8836330670325836 2118 5 2.5799134912703217 2118 6 1.4965020837378198 2118 7 5.753275053272052 2118 8 -2.6468353529150086 2118 9 -0.3874292766970413 2118 10-11 1.2046471285723896 2118 12-13 3.148224933792431 2118 14-15 0.4708812940728784 2118 16-17 0.10141316334934203 2118 18-19 1.2479800384675102 2118 20-21 0.021908665174404263 2118 22-23 -0.12792978521018483 2118 24-25 1.5701087188067788 2118 26-27 2.6935484836505985 2118 28-29 -0.5915866926604565 2118 30-31 -2.1299707960783607 2118 32-33 1.0094293718640515 2118 34-35 3.4054745188257307 2118 36-37 3.0793262461026636 2118 38-39 2.2025826406351285 2118 40-41 1.136167162756447 2118 42-43 2.475725407487648 2118 44-45 0.9387991150380905 2118 46-47 0.35890693762975445 2118 48-49 1.2287691522918855 2118 50-51 2.1627130238840344 2118 52-53 -1.2753822848735474 2118 54-55 1.378087999669635 2118 56-57 4.323351727623326 2118 58-59 0.5013983574472434 2118 60-61 -0.5334408282782697 2118 62-63 1.5251841302215716 2118 64-65 0.43126074959733174 2118 66-67 -3.194768942243332 2118 68-69 1.1292003775208563 2118 70-71 -0.7858473344226553 2118 72-73 -0.723535639330148 2118 74-75 0.9564115225414476 2118 76 -0.9290843083971971 2119 1 -2.5769904645671673 2119 2 -1.5408886039779333 2119 3 -1.5314228047563212 2119 4 -2.9951548117552953 2119 5 -0.6685713572145282 2119 6 -3.067134279898543 2119 7 -4.870967370970369 2119 8 -3.9438050498847055 2119 9 -5.223792913060677 2119 10-11 -6.028686204760945 2119 12-13 -1.0214720359045373 2119 14-15 -3.1048762816846995 2119 16-17 -1.519798957862779 2119 18-19 -5.555050264562791 2119 20-21 -2.450818607552872 2119 22-23 -2.121869179149577 2119 24-25 -6.935951887253829 2119 26-27 -3.29736060725849 2119 28-29 -2.821889722963487 2119 30-31 -3.6754253415329075 2119 32-33 -4.302691840257161 2119 34-35 -4.261192147840934 2119 36-37 -1.8146131478367309 2119 38-39 -2.958023419970928 2119 40-41 -1.748681322092036 2119 42-43 -4.3879109561487155 2119 44-45 -1.4824130061740313 2119 46-47 -5.132002153279338 2119 48-49 -2.407594484071751 2119 50-51 -0.3251657639947503 2119 52-53 -3.6875034969947613 2119 54-55 -3.8643362427546073 2119 56-57 -2.1736179693463704 2119 58-59 -0.6379955819466971 2119 60-61 -4.027380222217662 2119 62-63 -2.035421930384487 2119 64-65 -3.559648341311757 2119 66-67 -4.8432537907281805 2119 68-69 -2.1647390164185367 2119 70-71 -2.9585746071499273 2119 72-73 -4.55383866963318 2119 74-75 -2.6466187804888577 2119 76 1.1133399340270458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 7.0 13 89.0 14 306.0 15 552.0 16 823.0 17 926.0 18 904.0 19 803.0 20 683.0 21 505.0 22 379.0 23 299.0 24 233.0 25 218.0 26 256.0 27 246.0 28 284.0 29 349.0 30 405.0 31 513.0 32 668.0 33 800.0 34 1201.0 35 1596.0 36 2212.0 37 1185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.2017608217168 36.310833944729765 9.073123012961604 29.41428222059183 2 13.805266678829897 25.554947394027856 27.97543027428085 32.664355652861396 3 12.966003770601473 26.862494678586636 24.867724867724867 35.30377668308703 4 10.08331812929514 22.495894909688012 27.659186279875936 39.76160068114091 5 9.183239068296539 40.12041598248495 27.172657057714527 23.523687891503982 6 23.25609681931521 35.27945022197896 15.124977193942712 26.339475764763122 7 17.38733807699325 31.338563522471567 16.292647327130087 34.9814510734051 8 15.5020373411178 40.984005351821445 16.13452532992763 27.37943197713313 9 17.86170406860062 25.11707109408259 17.423827768655357 39.59739706866144 10-11 17.45423584504044 28.90895821930305 23.700054734537492 29.936751201119016 12-13 17.004196314541144 26.035395000912242 24.423766952502586 32.53664173204403 14-15 17.572827342942286 27.683512740984007 21.738733807699326 33.004926108374384 16-17 19.91424922459405 28.714346530438483 22.39858906525573 28.97281517971173 18-19 18.03807091163413 27.683512740984007 22.720914674937664 31.5575016724442 20-21 16.675789089582192 28.544061302681996 23.371647509578544 31.40850209815727 22-23 20.753512132822475 28.215654077723045 21.760019461168888 29.270814328285592 24-25 19.205741044821504 30.52058626771271 21.70224411603722 28.57142857142857 26-27 16.621054552089035 31.39025725232622 24.56060329623548 27.42808489934927 28-29 19.51894423158791 28.69306087696892 26.09317034604391 25.694824545399257 30-31 19.19965942954449 27.862920391656026 26.710454296661197 26.2269658821383 32-33 17.30827707839202 31.137870218329986 25.34513166697075 26.208721036307242 34-35 17.31739950130755 29.869853433071825 25.308641975308642 27.504105090311988 36-37 17.858663260962114 28.541020495043483 26.21480265158426 27.385513592410142 38-39 17.648847533905005 26.42765918627988 27.87204281457155 28.051450465243573 40-41 16.602809706257982 26.987167791765494 27.169616250076018 29.240406251900502 42-43 15.067201848811044 26.357720610594175 29.05187617831296 29.52320136228182 44-45 15.559812686249469 27.662227087514445 29.112692331083135 27.66526789515295 46-47 16.061545946603417 28.860305297086907 26.658760566806546 28.41938818950313 48-49 15.386486650854467 29.909383932372442 25.168764823937238 29.535364592835855 50-51 15.246609499483062 28.50148999574287 25.418111050294957 30.83378945447911 52-53 15.112813963388676 28.163960347868393 26.287782034908467 30.43544365383446 54-55 15.194915769628414 28.963692756796206 25.03801009548136 30.80338137809402 56-57 16.5055038618257 28.294715076324273 24.226114455999515 30.973666605850514 58-59 15.921668795232014 27.546676397251108 25.044091710758376 31.487563096758496 60-61 15.857811834823329 27.054065559812685 24.46025664416469 32.62786596119929 62-63 15.763546798029557 27.73216566320015 24.74305175454601 31.76123578422429 64-65 16.432524478501488 28.550142917959008 22.833424557562488 32.18390804597701 66-67 15.623669646658152 30.00973058444323 21.860366113239678 32.506233655658946 68-69 16.37778994100833 28.936325488049626 22.620568022866873 32.06531654807517 70-71 16.96466581524053 29.21303898315393 22.912485556163716 30.909809645441825 72-73 16.614972936812016 28.27342942285471 23.043240284619596 32.06835735571368 74-75 17.776561454722376 27.768655354862254 22.59928236939731 31.85550082101806 76 18.561089825457643 24.648786717752238 25.044091710758376 31.746031746031743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 2.0 16 3.0 17 3.0 18 5.0 19 6.5 20 9.0 21 12.0 22 20.0 23 24.0 24 22.0 25 24.0 26 39.5 27 62.0 28 75.0 29 81.0 30 108.5 31 158.5 32 205.5 33 230.0 34 242.5 35 333.0 36 444.5 37 478.0 38 540.5 39 642.0 40 739.5 41 817.0 42 836.0 43 923.5 44 1010.0 45 1058.5 46 1108.0 47 1075.5 48 1040.5 49 1000.5 50 963.0 51 924.5 52 847.5 53 741.5 54 674.0 55 626.0 56 548.0 57 496.5 58 475.0 59 407.5 60 298.0 61 226.0 62 196.0 63 171.5 64 133.5 65 110.5 66 79.0 67 57.0 68 54.0 69 38.5 70 26.5 71 27.0 72 22.5 73 18.5 74 15.0 75 11.0 76 8.5 77 4.0 78 3.5 79 5.0 80 4.0 81 4.0 82 3.0 83 1.0 84 2.0 85 1.5 86 1.0 87 2.0 88 1.5 89 1.5 90 1.5 91 2.0 92 3.0 93 2.5 94 2.0 95 1.5 96 1.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5291005291005291 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 16443.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.51006507328347 #Duplication Level Percentage of deduplicated Percentage of total 1 93.08167741077956 85.17910356990816 2 5.310028577125008 9.718421212674086 3 1.1497308433574798 3.156358328772122 4 0.3057087791586363 1.119017210971234 5 0.09968764537781619 0.45612114577631824 6 0.02658337210075098 0.1459587666484218 7 0.0 0.0 8 0.019937529075563235 0.1459587666484218 9 0.0 0.0 >10 0.006645843025187745 0.07906099860122849 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012163230554035152 0.0 2 0.0 0.0 0.0 0.012163230554035152 0.0 3 0.0 0.0 0.0 0.012163230554035152 0.0 4 0.0 0.0 0.0 0.012163230554035152 0.0 5 0.0 0.0 0.0 0.012163230554035152 0.0 6 0.0 0.0 0.0 0.012163230554035152 0.0 7 0.0 0.0 0.0 0.012163230554035152 0.0 8 0.0 0.0 0.0 0.012163230554035152 0.0 9 0.0 0.0 0.0 0.01824484583105273 0.0 10 0.0 0.0 0.0 0.01824484583105273 0.0 11 0.0 0.0 0.0 0.01824484583105273 0.0 12 0.0 0.0 0.0 0.01824484583105273 0.0 13 0.0 0.0 0.0 0.024326461108070303 0.0 14 0.0 0.0 0.0 0.024326461108070303 0.0 15 0.0 0.0 0.0 0.024326461108070303 0.0 16 0.0 0.0 0.0 0.024326461108070303 0.0 17 0.0 0.0 0.0 0.024326461108070303 0.0 18 0.0 0.0 0.0 0.024326461108070303 0.0 19 0.0 0.0 0.0 0.03040807638508788 0.0 20 0.0 0.0 0.0 0.03648969166210546 0.0 21 0.0 0.0 0.0 0.03648969166210546 0.0 22 0.0 0.0 0.0 0.03648969166210546 0.0 23 0.0 0.0 0.0 0.04257130693912303 0.0 24 0.0 0.0 0.0 0.04257130693912303 0.0 25 0.0 0.0 0.0 0.05473453749315818 0.0 26 0.0 0.0 0.0 0.05473453749315818 0.0 27 0.0 0.0 0.0 0.07297938332421092 0.0 28 0.0 0.0 0.0 0.07297938332421092 0.0 29 0.0 0.0 0.0 0.08514261387824607 0.0 30 0.0 0.0 0.0 0.09730584443228121 0.0 31 0.0 0.0 0.0 0.11555069026333394 0.0 32 0.0 0.0 0.0 0.13379553609438666 0.0 33 0.0 0.0 0.0 0.13987715137140425 0.0 34 0.0 0.0 0.0 0.18244845831052728 0.0 35 0.0 0.0 0.0 0.23718299580368546 0.0 36 0.0 0.0 0.0 0.29191753329684367 0.0 37 0.0 0.0 0.0 0.3831417624521073 0.0 38 0.0 0.0 0.0 0.5959982971477225 0.0 39 0.0 0.0 0.0 0.7297938332421091 0.0 40 0.0 0.0 0.0 0.8392629082284254 0.0 41 0.0 0.0 0.0 1.1798333637414098 0.0 42 0.0 0.0 0.0 1.5325670498084292 0.0 43 0.0 0.0 0.0 1.8609742747673783 0.0 44 0.0 0.0 0.0 2.1832998844493097 0.0 45 0.0 0.0 0.0 2.6272577996715927 0.0 46 0.0 0.0 0.0 3.0347260232317703 0.0 47 0.0 0.0 0.0 3.338806787082649 0.0 48 0.0 0.0 0.0 3.697622088426686 0.0 49 0.0 0.0 0.0 4.050355774493705 0.0 50 0.0 0.0 0.0 4.378762999452655 0.0 51 0.0 0.0 0.0 4.61594599525634 0.0 52 0.0 0.0 0.0 5.047740679924588 0.0 53 0.0 0.0 0.0 5.449127288207748 0.0 54 0.0 0.0 0.0 5.734963206227574 0.0 55 0.0 0.0 0.0 6.112023353402663 0.0 56 0.0 0.0 0.0 6.501246731131789 0.0 57 0.0 0.0 0.0 6.87222526302986 0.0 58 0.0 0.0 0.0 7.328346408806179 0.0 59 0.0 0.0 0.0 7.693243325427233 0.0 60 0.0 0.0 0.0 8.064221857325306 0.0 61 0.0 0.0 0.0 8.42911877394636 0.0 62 0.0 0.0 0.0 8.921729611384784 0.0 63 0.0 0.0 0.0 9.329197834944962 0.0 64 0.0 0.0 0.0 9.645441829349876 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCACA 45 0.004187855 31.109756 5 CCGACGA 80 8.173028E-5 26.248857 70 TGCCGAC 85 1.234202E-4 24.704807 68 GCCGACG 85 1.234202E-4 24.704807 69 CTGCCGA 90 1.8190859E-4 23.332317 67 GCTGCCG 95 2.623757E-4 22.104301 66 ACGCTGC 120 5.2923224E-5 20.415777 64 GACGCTG 120 5.2923224E-5 20.415777 63 TGACGCT 125 7.24622E-5 19.599148 62 TCTGACG 140 1.728882E-4 17.499239 60 CGCTGCC 120 0.0012666406 17.499237 65 CTGACGC 145 2.2611179E-4 16.895815 61 ATCTGAC 165 6.0507475E-4 14.847838 59 CATCTGA 170 7.588747E-4 14.411138 58 ACATCTG 195 1.2853261E-4 14.358349 57 CACATCT 205 0.0031125634 11.950699 56 TACACAT 220 0.0052772956 11.135879 54 GTCTCTT 225 0.0062393453 10.888414 46 TTATACA 230 0.007347696 10.6517105 51 ACACATC 235 0.008620294 10.425077 55 >>END_MODULE