FastQCFastQC Report
Wed 25 May 2016
SRR1780432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16443
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT168110.223195280666545No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC6994.251049078635286No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT5743.4908471690080884No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1921.1676701331873744No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT1380.8392629082284254No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG600.36489691662105456No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC590.35881530134403694No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG490.29799914857386123No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC480.2919175332968436No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG450.27367268746579093No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT400.24326461108070302No Hit
CTTATCACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC380.23110138052666787No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT330.20069330414158No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC320.19461168886456243No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT310.18853007358754487No Hit
ACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCT300.18244845831052728TruSeq Adapter, Index 25 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTCTGCCGTCTT280.17028522775649213No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT270.16420361247947454No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG260.15812199720245698No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT220.13379553609438666No Hit
CTTATACACATCTCCGAGCCCACGCGACTGCTGGGTATCTCGTATGCCGT210.1277139208173691No Hit
ATACACATCTCCGAGCCCACGAGAATGCTGGGTATCTCGTATGCCGTCTT200.12163230554035151No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC190.11555069026333394No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTC180.10946907498631638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG207.698276E-570.020
CTTTATA150.00218589870.02
TGATCTG150.00218589870.044
ATAACAC207.698276E-570.04
CGAGCAC207.698276E-570.015
TCTTTAT150.00218589870.01
GCACACG207.698276E-570.018
CTTTACA950.062.6315771
TTTACAC1000.059.4999962
TTATAAC252.3219835E-455.9999962
CTTATAA252.3219835E-455.9999961
TAACACA200.0068310852.55
TTACACA1200.049.5833323
CTATACA505.714537E-848.9999961
CACACGA305.710609E-446.66666419
TATAACA305.710609E-446.6666643
GAGCACA305.710609E-446.66666416
CCGAGCA305.710609E-446.66666414
AGCACAC305.710609E-446.66666417
TTTATAC305.710609E-446.6666643
Produced by FastQC (version 0.11.5)