##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16443 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3448275862069 34.0 34.0 34.0 27.0 34.0 2 32.92914918202275 34.0 34.0 34.0 31.0 34.0 3 33.30438484461473 34.0 34.0 34.0 33.0 34.0 4 33.33509700176367 34.0 34.0 34.0 33.0 34.0 5 33.3236027488901 34.0 34.0 34.0 33.0 34.0 6 36.89521376877699 38.0 37.0 38.0 35.0 38.0 7 36.544122118834764 38.0 37.0 38.0 34.0 38.0 8 36.58450404427416 38.0 37.0 38.0 34.0 38.0 9 36.27738247278477 38.0 37.0 38.0 34.0 38.0 10-11 35.897616006811404 38.0 36.5 38.0 32.5 38.0 12-13 35.77914614121511 38.0 36.0 38.0 31.5 38.0 14-15 35.875509335279446 38.0 36.0 38.0 32.0 38.0 16-17 36.092136471446814 38.0 36.5 38.0 32.5 38.0 18-19 36.17806969531107 38.0 37.0 38.0 33.5 38.0 20-21 35.94356261022928 38.0 36.0 38.0 31.5 38.0 22-23 35.80052301891382 38.0 36.5 38.0 32.0 38.0 24-25 34.74393358876118 37.0 34.5 38.0 28.0 38.0 26-27 35.15024630541872 37.0 35.5 38.0 29.0 38.0 28-29 35.035455817065014 37.5 35.5 38.0 29.0 38.0 30-31 34.559539013562 37.0 34.0 38.0 26.5 38.0 32-33 35.00988262482515 37.0 35.5 38.0 27.0 38.0 34-35 34.482910661071585 37.0 34.0 38.0 26.5 38.0 36-37 34.573222647935296 37.0 34.0 38.0 26.5 38.0 38-39 34.422945934440186 37.0 34.0 38.0 26.5 38.0 40-41 34.1602809706258 37.0 34.0 38.0 25.5 38.0 42-43 34.88259441707717 37.0 35.0 38.0 29.0 38.0 44-45 35.200024326461104 37.0 35.5 38.0 29.0 38.0 46-47 35.33363741409718 37.5 36.0 38.0 31.0 38.0 48-49 34.82059234932798 37.0 35.0 38.0 28.0 38.0 50-51 35.08933892841939 37.0 35.5 38.0 29.0 38.0 52-53 34.818828680897646 37.0 35.0 38.0 27.0 38.0 54-55 35.08982545764155 37.0 35.5 38.0 27.0 38.0 56-57 35.113513349145535 37.0 35.5 38.0 27.0 38.0 58-59 35.196284133065745 37.0 36.0 38.0 28.0 38.0 60-61 34.77674390318069 37.0 35.0 38.0 26.0 38.0 62-63 35.35565286139999 37.0 36.0 38.0 31.0 38.0 64-65 35.35641306330962 38.0 36.0 38.0 31.0 38.0 66-67 33.978258225384664 37.0 34.0 38.0 23.5 38.0 68-69 31.688165176670925 36.5 29.0 38.0 11.0 38.0 70-71 30.169099312777476 35.5 24.0 38.0 11.0 38.0 72-73 29.09219728759959 34.0 22.0 38.0 11.0 38.0 74-75 29.44897524782582 35.0 23.0 38.0 11.0 38.0 76 29.105941738125647 34.0 22.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08725138512691899 1101 2 1.1576395345410972 1101 3 0.503853495630807 1101 4 0.35362947064675154 1101 5 0.4779461586746194 1101 6 -1.2076809996874474 1101 7 0.5561877627542984 1101 8 1.0457232478472918 1101 9 0.6887117735938517 1101 10-11 0.866518013542894 1101 12-13 1.1406628802050705 1101 14-15 1.1528809227918941 1101 16-17 -0.09104756091109323 1101 18-19 -1.1105407776146663 1101 20-21 -0.3966304453120699 1101 22-23 0.27338832787045675 1101 24-25 -2.2328275969085922 1101 26-27 0.25794177898847437 1101 28-29 1.7835131858299036 1101 30-31 2.1216711414451765 1101 32-33 0.8454935783976225 1101 34-35 1.1762656039869057 1101 36-37 1.3619511145689813 1101 38-39 -0.017760710953744763 1101 40-41 0.9109224783946033 1101 42-43 1.7772613794302714 1101 44-45 0.7115714000892908 1101 46-47 1.3552109817169224 1101 48-49 -1.4617779503227055 1101 50-51 -2.5469070801996025 1101 52-53 -2.4652032392288916 1101 54-55 -1.5432270631298621 1101 56-57 -1.8626388697776548 1101 58-59 -2.244699207326633 1101 60-61 -2.548041911800027 1101 62-63 -0.6610475535996443 1101 64-65 -1.6026642845230157 1101 66-67 -1.4941634148299343 1101 68-69 -0.8185305440234671 1101 70-71 0.1544246024525222 1101 72-73 -1.4272544562824194 1101 74-75 -1.7030935317433418 1101 76 0.01742847352162258 1102 1 -0.3399415973292186 1102 2 0.5664114643656575 1102 3 0.4845552500167756 1102 4 0.4922259618748228 1102 5 0.15952510604304138 1102 6 0.6914418073301007 1102 7 0.28864390310517507 1102 8 1.0562495636367686 1102 9 1.0825714227166543 1102 10-11 1.1774829258235968 1102 12-13 1.3884698977489336 1102 14-15 0.2787581157743517 1102 16-17 -0.11955633284091505 1102 18-19 -0.12062849691290722 1102 20-21 0.8652116599510862 1102 22-23 0.9650549945371196 1102 24-25 -5.094669702171746 1102 26-27 -0.8596020806606575 1102 28-29 0.8449166946018352 1102 30-31 2.6150921940767518 1102 32-33 0.27224796436253484 1102 34-35 1.1157392881974317 1102 36-37 -2.500329587185405 1102 38-39 -0.44802386884848033 1102 40-41 0.4503961626051378 1102 42-43 0.9101561162723826 1102 44-45 1.6523608737735032 1102 46-47 1.3951232624186787 1102 48-49 -2.9819533889191945 1102 50-51 -0.2214684837083709 1102 52-53 0.08567395375356313 1102 54-55 0.9129132877473296 1102 56-57 0.9579751653100601 1102 58-59 -0.003909733642416313 1102 60-61 -8.351550683729851 1102 62-63 -2.8518370272838567 1102 64-65 0.565318171617335 1102 66-67 2.1067137781525247 1102 68-69 -1.3413375615673253 1102 70-71 -1.4368034677229176 1102 72-73 -4.492166736984174 1102 74-75 -3.2759005492872006 1102 76 -1.1755539826187267 1103 1 1.7167250693374498 1103 2 1.3064114643656595 1103 3 0.6245552500167761 1103 4 0.7122259618748217 1103 5 0.6828584393763748 1103 6 0.9747751406634322 1103 7 1.5919772364385096 1103 8 1.2029162303034369 1103 9 1.2992380893833229 1103 10-11 0.8124829258235948 1103 12-13 0.24346989774893046 1103 14-15 -0.42290855089231627 1103 16-17 0.25211033382575465 1103 18-19 1.1343715030870882 1103 20-21 0.19354499328442643 1103 22-23 1.1933883278704513 1103 24-25 -2.983003035505078 1103 26-27 0.09373125267267568 1103 28-29 0.7515833612685014 1103 30-31 2.290092194076756 1103 32-33 2.1939146310292017 1103 34-35 1.3524059548641034 1103 36-37 -0.9836629205187393 1103 38-39 0.4369761311515177 1103 40-41 0.8937294959384658 1103 42-43 0.20848944960571458 1103 44-45 1.487360873773504 1103 46-47 1.886789929085353 1103 48-49 -1.3986200555858659 1103 50-51 0.5218648496249614 1103 52-53 1.6456739537535654 1103 54-55 0.35291328774732733 1103 56-57 0.042975165310068064 1103 58-59 0.49775693302424884 1103 60-61 -4.758217350396521 1103 62-63 -1.0435036939505196 1103 64-65 0.690318171617335 1103 66-67 -0.7566195551808121 1103 68-69 -1.0280042282339892 1103 70-71 -1.6101368010562496 1103 72-73 -5.318833403650839 1103 74-75 -3.819233882620537 1103 76 -1.94888731595206 1104 1 0.20025448110214938 1104 2 0.5389604839734972 1104 3 0.2404376029579467 1104 4 0.24810831481599394 1104 5 -1.267925874349114 1104 6 0.063990826937939 1104 7 1.2984478246738078 1104 8 0.8837005440289261 1104 9 1.0904145599715562 1104 10-11 0.05150253366673496 1104 12-13 0.2512149957881462 1104 14-15 1.0380718412645464 1104 16-17 0.6819142553943749 1104 18-19 0.670547973675319 1104 20-21 0.4799175423040296 1104 22-23 0.4228981317920173 1104 24-25 0.275428337043941 1104 26-27 1.4707900762020927 1104 28-29 1.5238382632292797 1104 30-31 0.8896019979983194 1104 32-33 0.5202871800488111 1104 34-35 -0.8406332608221732 1104 36-37 1.6947684520302744 1104 38-39 1.95295652330838 1104 40-41 1.2616706724090605 1104 42-43 0.7880972927429681 1104 44-45 1.7381451874989935 1104 46-47 1.5162016937912313 1104 48-49 0.19843876794354287 1104 50-51 1.2878452417818238 1104 52-53 1.0964582674790577 1104 54-55 0.21438387598261954 1104 56-57 0.25552418491790263 1104 58-59 -1.5514587532502588 1104 60-61 -0.15253107588671355 1104 62-63 0.26826101193183405 1104 64-65 1.0854162108330172 1104 66-67 0.36896868011330497 1104 68-69 1.7988585168640512 1104 70-71 1.2734906499241418 1104 72-73 -0.7279510507096631 1104 74-75 -0.8013907453656373 1104 76 -1.9843775120304912 1105 1 1.39785182990083 1105 2 0.9668809479337312 1105 3 0.6437101795942368 1105 4 -0.23594305220968437 1105 5 0.14238895580830047 1105 6 0.30529157258831674 1105 7 1.4212730110863987 1105 8 -0.6921072438749718 1105 9 1.3076887936086763 1105 10-11 1.320558043194488 1105 12-13 -0.5440418393402737 1105 14-15 0.31126985286354625 1105 16-17 0.3927676108210534 1105 18-19 0.7416954467490555 1105 20-21 0.6078172937539037 1105 22-23 0.670101942893929 1105 24-25 1.1664805325700343 1105 26-27 1.1582382949261998 1105 28-29 1.896559887090092 1105 30-31 1.668495949945303 1105 32-33 2.155229185019813 1105 34-35 -0.003744279877679446 1105 36-37 0.49427135178172676 1105 38-39 -2.050253915796837 1105 40-41 0.6675323128398745 1105 42-43 0.2520575247230852 1105 44-45 0.8348726108626963 1105 46-47 1.8318603516205627 1105 48-49 -0.26904259079712745 1105 50-51 2.013508042113223 1105 52-53 1.4607913246455837 1105 54-55 1.9269977947895853 1105 56-57 1.3806277474696884 1105 58-59 0.9607616278599309 1105 60-61 1.560186405472031 1105 62-63 -0.43223608831671356 1105 64-65 1.9659050260774293 1105 66-67 1.4459156560867896 1105 68-69 1.6337798093246931 1105 70-71 1.8251683632630034 1105 72-73 -0.07291791069309639 1105 74-75 -0.38693341313696905 1105 76 0.21881221456437316 1106 1 -1.2361781564690055 1106 2 0.6234007116774833 1106 3 0.6716520242103243 1106 4 -0.0626127478025964 1106 5 -0.1754211305160993 1106 6 0.44681815141611736 1106 7 -1.289313086142137 1106 8 0.08958289697010002 1106 9 -1.1168909428747398 1106 10-11 0.427214108619296 1106 12-13 1.556480650437102 1106 14-15 -0.46667199175253415 1106 16-17 0.3199597961913412 1106 18-19 0.33824247082902303 1106 20-21 0.41279230511238296 1106 22-23 0.7518829515263619 1106 24-25 -0.1766589494835813 1106 26-27 1.2914731881565515 1106 28-29 0.508895189225484 1106 30-31 1.3960061725713828 1106 32-33 1.122516781566837 1106 34-35 1.5006855247565767 1106 36-37 2.0491327784059905 1106 38-39 -0.08807763228934107 1106 40-41 1.154697237873954 1106 42-43 1.169027084014317 1106 44-45 1.6617694759240393 1106 46-47 1.212596380698244 1106 48-49 2.002992847639945 1106 50-51 -0.4306082686546091 1106 52-53 0.26201803977507154 1106 54-55 -0.030635099349439088 1106 56-57 1.3077063481057607 1106 58-59 1.9931332771102745 1106 60-61 1.8925353377755272 1106 62-63 1.6548834028236712 1106 64-65 -1.3505420434364268 1106 66-67 -1.6459743938904907 1106 68-69 0.8140387825186934 1106 70-71 1.5260997580835323 1106 72-73 1.0701988544136753 1106 74-75 0.14452955823967883 1106 76 0.8567040818974014 1107 1 0.9382635308759077 1107 2 0.08692428487847792 1107 3 0.6614783269398501 1107 4 0.5768413464902054 1107 5 0.4223456188635524 1107 6 0.5388777047659943 1107 7 1.118131082592356 1107 8 0.49727520466240804 1107 9 -0.6084542183089852 1107 10-11 0.9037649771056451 1107 12-13 0.9429570772361089 1107 14-15 0.978117090133324 1107 16-17 0.9054436671590835 1107 18-19 0.7159099646255456 1107 20-21 1.3248270445664687 1107 22-23 -0.29327833879622034 1107 24-25 0.6098174773154312 1107 26-27 -0.1493456704042444 1107 28-29 0.9167115663966996 1107 30-31 0.6234255274100846 1107 32-33 2.211350528465097 1107 34-35 0.6990726215307674 1107 36-37 2.3599268230710067 1107 38-39 -0.005075150899763514 1107 40-41 0.749114111323081 1107 42-43 0.8120791931954585 1107 44-45 1.8863352327478609 1107 46-47 1.6790976213930406 1107 48-49 1.7244568674910568 1107 50-51 1.449044336804448 1107 52-53 0.8292636973433076 1107 54-55 1.0821440569780947 1107 56-57 1.557334139669038 1107 58-59 1.2275005227678406 1107 60-61 1.9997313675521937 1107 62-63 1.1488039983571738 1107 64-65 1.7082668895660476 1107 66-67 0.3156881371268767 1107 68-69 1.8484060281762638 1107 70-71 0.9324273015078539 1107 72-73 -0.6434487882662232 1107 74-75 -1.5079518313384845 1107 76 -0.3909385980033413 1108 1 -1.1535779609655847 1108 2 0.5755023734565654 1108 3 -0.018475053013531806 1108 4 0.41343808308694463 1108 5 0.13982813634607538 1108 6 -0.043406677518383674 1108 7 -0.0674167029554269 1108 8 -1.1528413454541422 1108 9 -0.49470130455606665 1108 10-11 0.4039980773387484 1108 12-13 1.6952880795671064 1108 14-15 0.9128490248652597 1108 16-17 -0.14607148435607087 1108 18-19 0.06649271520829814 1108 20-21 1.468999538738963 1108 22-23 0.0612671157492386 1108 24-25 1.2727545402524925 1108 26-27 -0.547480868539445 1108 28-29 -0.8065984569133207 1108 30-31 0.7249406789252433 1108 32-33 1.168460085574658 1108 34-35 0.5263453488034955 1108 36-37 2.010276473420653 1108 38-39 1.1951579493333355 1108 40-41 0.6019113141202794 1108 42-43 0.26848944960570975 1108 44-45 1.0046336010462298 1108 46-47 1.138305080600503 1108 48-49 1.5589557019898947 1108 50-51 1.7042890920492013 1108 52-53 0.15840122648084076 1108 54-55 -0.9658745910405457 1108 56-57 0.004187286522181921 1108 58-59 1.2226054178727281 1108 60-61 1.7832978011186285 1108 62-63 0.8034660030191816 1108 64-65 1.0827424140415758 1108 66-67 1.1824713539100955 1108 68-69 0.9601775899478255 1108 70-71 -1.6095307404501895 1108 72-73 -1.4982273430447783 1108 74-75 -0.8562035795902325 1108 76 -0.7210085280732734 1109 1 -2.4826499306625536 1109 2 0.2393281310323232 1109 3 -0.30919474998322727 1109 4 0.30785096187482 1109 5 0.3543167727097085 1109 6 0.014358473996765042 1109 7 0.6063522364385108 1109 8 0.8239578969701 1109 9 1.2742380893833243 1109 10-11 1.216024592490264 1109 12-13 0.8702407310822622 1109 14-15 1.1235497824410174 1109 16-17 1.060131167159085 1109 18-19 0.6903090030870871 1109 20-21 1.1917741599510876 1109 22-23 0.9973466612037853 1109 24-25 0.012101131161585954 1109 26-27 0.10498125267267966 1109 28-29 -1.4467499720648362 1109 30-31 1.2531130274100875 1109 32-33 1.9508937976958691 1109 34-35 0.21313512153076886 1109 36-37 1.1501912461479264 1109 38-39 1.108746964484851 1109 40-41 0.8384169959384664 1109 42-43 0.8882811162723812 1109 44-45 1.4752775404401675 1109 46-47 1.6274149290853472 1109 48-49 0.895129944414137 1109 50-51 -0.03292681704170519 1109 52-53 -0.6976593795797683 1109 54-55 -1.0480242122526704 1109 56-57 -0.028483168023271332 1109 58-59 0.08411109969091513 1109 60-61 0.8010534829368154 1109 62-63 0.918996306049479 1109 64-65 1.4861515049506693 1109 66-67 0.828067944819189 1109 68-69 -2.180920894900659 1109 70-71 -1.1493034677229161 1109 72-73 -1.4103959036508407 1109 74-75 -1.9925672159538692 1109 76 -2.0193039826187267 1110 1 -1.3540441614317835 1110 2 0.1792319771861699 1110 3 0.6499398654013859 1110 4 0.40761057725943317 1110 5 0.6992686957866283 1110 6 0.8196469355352249 1110 7 1.6604387749000509 1110 8 1.2818905892777934 1110 9 1.6107765509217842 1110 10-11 -1.2635427152020426 1110 12-13 0.5968032310822622 1110 14-15 1.4300401670564042 1110 16-17 0.668905205620618 1110 18-19 -0.4148592661436794 1110 20-21 1.5805962753357008 1110 22-23 -0.749047569565441 1110 24-25 -1.3190286765307206 1110 26-27 1.0545004834419132 1110 28-29 -0.18713458744944944 1110 30-31 -1.3111898572052993 1110 32-33 0.7075043746189493 1110 34-35 0.6163803138384623 1110 36-37 2.1868498999940797 1110 38-39 0.4314633106386978 1110 40-41 -1.5912705040615336 1110 42-43 0.15630996242622786 1110 44-45 2.0594121558247807 1110 46-47 1.9194822367776538 1110 48-49 1.801379944414137 1110 50-51 0.1798135675736816 1110 52-53 1.129263697343312 1110 54-55 0.230220980055023 1110 56-57 0.5881033704382688 1110 58-59 -0.7494225541552382 1110 60-61 0.07088521370604184 1110 62-63 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-0.7098840170631817 2116 62-63 1.7064963060494733 2116 64-65 -2.0763484950493307 2116 66-67 0.04838044481919468 2116 68-69 -1.2996708949006575 2116 70-71 1.4131965322770839 2116 72-73 0.41616659634916076 2116 74-75 1.182432784046128 2116 76 2.3244460173812733 2117 1 -1.417392577721376 2117 2 -0.4316277513206188 2117 3 0.31396701472265676 2117 4 0.14516713834540695 2117 5 0.5997211844744115 2117 6 -0.421303290709119 2117 7 -2.363316881208547 2117 8 0.6484064263818681 2117 9 -1.0419383812049077 2117 10-11 -2.11026217221562 2117 12-13 -1.6679026512706798 2117 14-15 -1.7413399234413305 2117 16-17 -3.347497509311509 2117 18-19 -2.175040261618797 2117 20-21 -1.0421412812253834 2117 22-23 0.34201577885084333 2117 24-25 -0.22457166295605901 2117 26-27 -3.1615628649743783 2117 28-29 -4.086455854417778 2117 30-31 -2.3236332961193256 2117 32-33 -6.178242231715895 2117 34-35 0.004954974471942819 2117 36-37 -1.6287609597344286 2117 38-39 -0.5543964178680909 2117 40-41 -2.4956822687674176 2117 42-43 -1.388373295492329 2117 44-45 -0.6883254007363035 2117 46-47 -1.4837983062087687 2117 48-49 -2.669208290879979 2117 50-51 -1.4989194641005312 2117 52-53 -0.0064829089915363625 2117 54-55 -1.4694396534291414 2117 56-57 0.3143477143296707 2117 58-59 -1.50734110619144 2117 60-61 -1.4613546052984816 2117 62-63 -0.614091929244637 2117 64-65 -1.3704661421081568 2117 66-67 -0.976619555180811 2117 68-69 -0.45114148313595237 2117 70-71 -2.572097585369974 2117 72-73 1.5294018904668043 2117 74-75 2.742726901693189 2117 76 -3.7049657473246107 2118 1 0.07672506933744927 2118 2 -0.3335885356343411 2118 3 -4.015444749983228 2118 4 -0.14110737145851004 2118 5 -0.09047489395695862 2118 6 -1.0918915260032378 2118 7 -0.061356096894819245 2118 8 0.9229162303034357 2118 9 -1.76742857728334 2118 10-11 -0.8808504075097332 2118 12-13 -1.9365301022510693 2118 14-15 0.07042478244101602 2118 16-17 -3.561222999507578 2118 18-19 -1.4789618302462486 2118 20-21 -0.3264550067155767 2118 22-23 0.20672166120378677 2118 24-25 1.230330297828246 2118 26-27 -5.2262687473273175 2118 28-29 -0.05508330539817052 2118 30-31 -1.4765744725899168 2118 32-33 -0.2860853689708023 2118 34-35 0.6324059548641046 2118 36-37 -1.7836629205187364 2118 38-39 1.0769761311515182 2118 40-41 1.9670628292718035 2118 42-43 1.7018227829390469 2118 44-45 -5.539305792893167 2118 46-47 1.3534565957520144 2118 48-49 1.4013799444141384 2118 50-51 0.32853151629162625 2118 52-53 -2.514326046246431 2118 54-55 -2.5204200455860075 2118 56-57 -0.4170248346899399 2118 58-59 0.6710902663575808 2118 60-61 -0.18488401706319024 2118 62-63 -2.276837027283854 2118 64-65 1.2569848382839979 2118 66-67 1.7900471114858547 2118 68-69 -0.14133756156732602 2118 70-71 1.1631965322770839 2118 72-73 5.607833263015824 2118 74-75 2.4074327840461294 2118 76 1.8911126840479398 2119 1 0.8767250693374464 2119 2 -0.7578309598767667 2119 3 0.23910070456222599 2119 4 -0.4805013108524534 2119 5 -0.314717318199385 2119 6 0.10810847399676504 2119 7 0.6901590546203309 2119 8 1.2259465333337332 2119 9 0.9901471802924178 2119 10-11 -0.5353958620551893 2119 12-13 -1.7668331325540976 2119 14-15 -0.8750297630135293 2119 16-17 0.6418073035227252 2119 18-19 -1.0092648605492727 2119 20-21 -0.8416065218670923 2119 22-23 -0.5750965206144016 2119 24-25 -2.2272454597475075 2119 26-27 1.346458525399953 2119 28-29 0.7237045733897105 2119 30-31 1.1416073455919005 2119 32-33 1.2442176613322289 2119 34-35 1.1172544397125819 2119 36-37 -0.7624507993066203 2119 38-39 -1.107872353696969 2119 40-41 0.4428204050293729 2119 42-43 -0.0193893382730721 2119 44-45 1.784936631349261 2119 46-47 0.9413353836308005 2119 48-49 1.6650163080504967 2119 50-51 1.431561819321935 2119 52-53 -1.493113925034315 2119 54-55 0.7310951059291426 2119 56-57 -0.3139945316596311 2119 58-59 0.13169632696364175 2119 60-61 2.5787523465731823 2119 62-63 -0.32532187576870797 2119 64-65 1.5145605958597628 2119 66-67 2.4324713539100955 2119 68-69 2.6798745596447944 2119 70-71 3.299560168640717 2119 72-73 1.5320756872582493 2119 74-75 2.325614602227944 2119 76 3.8244460173812733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 5.0 24 19.0 25 39.0 26 100.0 27 160.0 28 259.0 29 420.0 30 667.0 31 949.0 32 1349.0 33 2014.0 34 2470.0 35 2592.0 36 2880.0 37 2518.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.272577996715928 28.401143343672082 10.68539804171988 34.64088061789211 2 13.501185914979018 16.231831174359908 51.7971173143587 18.46986559630238 3 13.051146384479717 33.22386425834702 36.38630420239616 17.33868515477711 4 9.317034604390926 21.814753998662045 33.99622939852825 34.871981998418775 5 9.1345861460804 47.248069087149545 31.362889983579638 12.254454783190416 6 18.755701514322205 33.7043118652314 15.02767134951043 32.51231527093596 7 17.344766770054125 44.65730097914006 16.92513531593991 21.0727969348659 8 15.945995256340083 32.36027488901052 27.99975673538892 23.693973119260477 9 15.222283038374993 22.90336313324819 16.469014170163597 45.40533965821322 10-11 15.970321717448154 22.13099799306696 32.42717265705771 29.471507632427173 12-13 22.997628170041963 17.43295019157088 26.49151614668856 33.077905491698594 14-15 26.49759776196558 25.21741774615338 18.022866873441586 30.26211761843946 16-17 23.566259198443106 26.5401690689047 17.05589004439579 32.837681688256396 18-19 19.050659855257557 27.90853250623366 18.199233716475096 34.84157392203369 20-21 22.237426260414768 23.63011615885179 18.199233716475096 35.933223864258345 22-23 25.95937480994952 31.10442133430639 18.442498327555796 24.493705528188286 24-25 23.192239858906525 34.33679985404123 18.667518092805448 23.80344219424679 26-27 25.770844736361976 28.094021772182693 26.03843580855075 20.09669768290458 28-29 19.686188651705894 20.583226905065985 33.3880678708265 26.34251657240163 30-31 32.8011919965943 18.342151675485006 25.5367025481968 23.319953779723896 32-33 34.312473392933164 19.202700237182995 29.57793589977498 16.90689047010886 34-35 27.455452168095846 26.49151614668856 30.395913154533844 15.65711853068175 36-37 17.31435869366904 26.081007115489875 34.39761600681141 22.20701818402968 38-39 21.428571428571427 20.802165055038618 28.039287234689535 29.72997628170042 40-41 19.0993127774737 25.34513166697075 31.703460439092623 23.85209511646293 42-43 25.21133613087636 23.316912972085387 34.60439092623 16.867359970808245 44-45 19.683147844067385 22.474609256218454 27.926777352064708 29.915465547649454 46-47 26.220884266861276 18.749619899045186 27.902450890956636 27.127044943136898 48-49 21.70224411603722 18.284376330353343 31.393298059964724 28.62008149364471 50-51 15.65711853068175 18.19315210119808 35.741652983032296 30.408076385087877 52-53 20.525451559934318 18.223560177583167 39.363254880496264 21.887733381986255 54-55 17.782643069999395 18.272213099799306 35.84504044274159 28.100103387459708 56-57 24.195706379614425 18.39080459770115 33.20257860487745 24.210910417806968 58-59 18.083683026211762 32.98972207018184 30.63613695797604 18.290457945630358 60-61 17.229216079790792 44.31673052362707 21.887733381986255 16.566320014595874 62-63 13.102840114334366 50.92136471446816 19.056741470534575 16.919053700662896 64-65 12.984248616432525 51.80928054491273 18.652314054612905 16.55415678404184 66-67 12.966003770601473 50.5868758742322 18.217478562306148 18.229641792860185 68-69 13.042023961564192 46.34190841087393 19.17229216079791 21.443775466763974 70-71 13.44949218512437 42.54393967037645 20.09365687526607 23.91291126923311 72-73 14.410387398893146 38.48142066532871 20.862981207808794 26.245210727969347 74-75 14.869549352307972 37.906708021650545 20.67141032658274 26.552332299458737 76 14.62628474122727 38.794623852095114 20.409900869670984 26.16919053700663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.5 17 2.0 18 2.5 19 6.0 20 10.0 21 11.0 22 10.0 23 16.0 24 25.5 25 28.0 26 32.5 27 45.5 28 69.5 29 85.0 30 98.5 31 129.0 32 181.0 33 216.0 34 248.5 35 313.5 36 361.5 37 377.0 38 421.0 39 534.5 40 730.0 41 1144.0 42 1432.0 43 1760.5 44 2101.5 45 1899.0 46 1684.0 47 1544.0 48 1199.5 49 828.0 50 661.0 51 598.5 52 495.5 53 404.0 54 353.0 55 356.5 56 284.0 57 178.0 58 148.0 59 130.0 60 91.5 61 65.5 62 60.0 63 48.5 64 31.0 65 19.5 66 12.0 67 10.0 68 7.5 69 3.5 70 1.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 16443.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.46950069938576 #Duplication Level Percentage of deduplicated Percentage of total 1 83.07351905863422 50.23414218816518 2 11.948104193905259 14.449917898193759 3 2.8562808005632103 5.181536216018975 4 0.9956753494921051 2.40831964969896 5 0.3218344564014885 0.9730584443228121 6 0.1910892084883838 0.6933041415800036 7 0.10057326762546515 0.4257130693912303 8 0.07040128733782561 0.34057045551298426 9 0.04022930705018606 0.21893814997263275 >10 0.331891783164035 4.378762999452655 >50 0.02011465352509303 0.7237122179650916 >100 0.02011465352509303 2.0069330414157998 >500 0.02011465352509303 7.741896247643375 >1k 0.010057326762546516 10.223195280666545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 1681 10.223195280666545 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 699 4.251049078635286 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 574 3.4908471690080884 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 192 1.1676701331873744 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 138 0.8392629082284254 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 60 0.36489691662105456 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 59 0.35881530134403694 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 49 0.29799914857386123 No Hit CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 48 0.2919175332968436 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 45 0.27367268746579093 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 40 0.24326461108070302 No Hit CTTATCACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 38 0.23110138052666787 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT 33 0.20069330414158 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 32 0.19461168886456243 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 31 0.18853007358754487 No Hit ACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCT 30 0.18244845831052728 TruSeq Adapter, Index 25 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTCTGCCGTCTT 28 0.17028522775649213 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT 27 0.16420361247947454 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 26 0.15812199720245698 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 22 0.13379553609438666 No Hit CTTATACACATCTCCGAGCCCACGCGACTGCTGGGTATCTCGTATGCCGT 21 0.1277139208173691 No Hit ATACACATCTCCGAGCCCACGAGAATGCTGGGTATCTCGTATGCCGTCTT 20 0.12163230554035151 No Hit TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 19 0.11555069026333394 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTC 18 0.10946907498631638 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012163230554035152 0.0 2 0.0 0.0 0.0 0.012163230554035152 0.0 3 0.0 0.0 0.0 0.012163230554035152 0.0 4 0.0 0.0 0.0 0.012163230554035152 0.0 5 0.0 0.0 0.0 0.012163230554035152 0.0 6 0.0 0.0 0.0 0.012163230554035152 0.0 7 0.0 0.0 0.0 0.012163230554035152 0.0 8 0.0 0.0 0.0 0.012163230554035152 0.0 9 0.0 0.0 0.0 0.012163230554035152 0.0 10 0.0 0.0 0.0 0.012163230554035152 0.0 11 0.0 0.0 0.0 0.012163230554035152 0.0 12 0.0 0.0 0.0 0.012163230554035152 0.0 13 0.0 0.0 0.0 0.01824484583105273 0.0 14 0.0 0.0 0.0 0.01824484583105273 0.0 15 0.0 0.0 0.0 0.01824484583105273 0.0 16 0.0 0.0 0.0 0.01824484583105273 0.0 17 0.0 0.0 0.0 0.01824484583105273 0.0 18 0.0 0.0 0.0 0.01824484583105273 0.0 19 0.0 0.0 0.0 0.03648969166210546 0.0 20 0.0 0.0 0.0 0.04257130693912303 0.0 21 0.0 0.0 0.0 0.04257130693912303 0.0 22 0.0 0.0 0.0 0.04865292221614061 0.0 23 0.0 0.0 0.0 0.04865292221614061 0.0 24 0.0 0.0 0.0 0.04865292221614061 0.0 25 0.0 0.0 0.0 0.06081615277017576 0.0 26 0.0 0.0 0.0 0.06081615277017576 0.0 27 0.0 0.0 0.0 0.07906099860122849 0.0 28 0.0 0.0 0.0 0.07906099860122849 0.0 29 0.0 0.0 0.0 0.09730584443228121 0.0 30 0.0 0.0 0.0 0.10946907498631636 0.0 31 0.0 0.0 0.0 0.12163230554035152 0.0 32 0.0 0.0 0.0 0.14595876664842183 0.0 33 0.0 0.0 0.0 0.15812199720245698 0.0 34 0.0 0.0 0.0 0.20677491941859757 0.0 35 0.0 0.0 0.0 0.24326461108070305 0.0 36 0.0 0.0 0.0 0.29799914857386123 0.0 37 0.0 0.0 0.0 0.3953049930061424 0.0 38 0.0 0.0 0.0 0.6203247582557927 0.0 39 0.0 0.0 0.0 0.717630602688074 0.0 40 0.0 0.0 0.0 0.8575077540594782 0.0 41 0.0 0.0 0.0 1.2102414401264976 0.0 42 0.0 0.0 0.0 1.6116280484096577 0.0 43 0.0 0.0 0.0 1.9278720428145715 0.0 44 0.0 0.0 0.0 2.292768959435626 0.0 45 0.0 0.0 0.0 2.736726874657909 0.0 46 0.0 0.0 0.0 3.150276713495104 0.0 47 0.0 0.0 0.0 3.466520707900018 0.0 48 0.0 0.0 0.0 3.8374992397980905 0.0 49 0.0 0.0 0.0 4.196314541142128 0.0 50 0.0 0.0 0.0 4.524721766101076 0.0 51 0.0 0.0 0.0 4.774067992458797 0.0 52 0.0 0.0 0.0 5.230189138235115 0.0 53 0.0 0.0 0.0 5.674147053457398 0.0 54 0.0 0.0 0.0 6.002554278416348 0.0 55 0.0 0.0 0.0 6.416104117253543 0.0 56 0.0 0.0 0.0 6.811409110259685 0.0 57 0.0 0.0 0.0 7.212795718542845 0.0 58 0.0 0.0 0.0 7.650672018488111 0.0 59 0.0 0.0 0.0 8.058140242048289 0.0 60 0.0 0.0 0.0 8.435200389223377 0.0 61 0.0 0.0 0.0 8.85483184333759 0.0 62 0.0 0.0 0.0 9.329197834944962 0.0 63 0.0 0.0 0.0 9.712339597397069 0.0 64 0.0 0.0 0.0 10.071154898741106 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGAG 20 7.698276E-5 70.0 20 CTTTATA 15 0.002185898 70.0 2 TGATCTG 15 0.002185898 70.0 44 ATAACAC 20 7.698276E-5 70.0 4 CGAGCAC 20 7.698276E-5 70.0 15 TCTTTAT 15 0.002185898 70.0 1 GCACACG 20 7.698276E-5 70.0 18 CTTTACA 95 0.0 62.631577 1 TTTACAC 100 0.0 59.499996 2 TTATAAC 25 2.3219835E-4 55.999996 2 CTTATAA 25 2.3219835E-4 55.999996 1 TAACACA 20 0.00683108 52.5 5 TTACACA 120 0.0 49.583332 3 CTATACA 50 5.714537E-8 48.999996 1 CACACGA 30 5.710609E-4 46.666664 19 TATAACA 30 5.710609E-4 46.666664 3 GAGCACA 30 5.710609E-4 46.666664 16 CCGAGCA 30 5.710609E-4 46.666664 14 AGCACAC 30 5.710609E-4 46.666664 17 TTTATAC 30 5.710609E-4 46.666664 3 >>END_MODULE