FastQCFastQC Report
Wed 25 May 2016
SRR1780431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319935
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCA250.002332611735.25395224
ATCGGAA250.002348813735.20425837
TGACGGA300.0057003329.37368613
TTATCGG300.005735372729.33688235
TATCGGA300.005735372729.33688236
CTATACA1053.6379788E-1227.2371251
TTATAAA508.4316154E-526.3495482
CGGACGA450.001373472624.51266921
CGTGACG450.001378508424.4972817
TGACGAC551.560252E-424.06698219
CCGTTTA703.171953E-522.03026613
ATGCATG602.8488867E-422.01990722
GCCGTTT703.1834956E-522.01990512
CTTATAA904.0637678E-721.9992181
AAGACTT500.002605103721.9579565
CCATTAG500.002605103721.9579564
GTAGAGC755.4514036E-520.53581838
GCGTGTA1502.0008883E-1120.53581834
TTTAAAC654.953703E-420.28790932
ATTTGCG1201.4299076E-820.15647930
Produced by FastQC (version 0.11.5)