FastQCFastQC Report
Wed 25 May 2016
SRR1780431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319935
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT3365910.520574491693626No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT248417.764389641645959Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC81602.550518073983778No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC41631.301201806616969No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG40221.2571303546032788No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT24190.7560910810008283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC20300.6345038836013566No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC14330.44790348039445516No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG11080.3463203463203463No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG7890.24661259318299028No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC7430.23223467266788567No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC5520.17253504618125556No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG5200.1625330145185741No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC5080.15878225264506854No Hit
CTTATCACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC4450.13909075280916436No Hit
CTTATTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG4240.13252691953052964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT26350.041.99232544
CTATACA4650.040.7417261
GCCGTCT28750.038.4868843
CTTTACA9050.037.9725651
CTTATAA1300.037.2797741
TTTACAC9150.037.270142
AGCACCG250.002351281535.19674718
TCGTTCG250.002351281535.19674723
TTCGGTA250.002351281535.19674726
CGGTAAG250.002351281535.19674728
TCTTTAT1950.032.761011
CTTATAC47150.031.4899581
TTATACA47950.030.879972
TTACACA11050.030.8617023
TCACATC502.3559896E-630.7971535
CGCATCT300.005732604329.33979615
CGCACTT300.005732604329.33979614
CTAATAT300.005736986629.335213
AACAACG300.005741372229.33062443
GAAGCGG300.005741372229.33062444
Produced by FastQC (version 0.11.5)