FastQCFastQC Report
Wed 25 May 2016
SRR1780430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76497
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG150.002171970.362921
GTTAGAG150.002217604369.994773
GTATGGA150.002217604369.9947720
TCGCTCA150.002217604369.9947759
CTGGAGA150.002217604369.994774
CTTGGTT252.8169397E-669.9947630
TGCTTGG308.3095E-658.3289728
GCTTGGT308.3095E-658.3289729
CCTTGCT308.3095E-658.3289715
CCTTGCG308.3095E-658.3289754
GATACTA200.006929411652.4960865
GAAGCGG200.006929411652.4960844
TTAGAGT200.006929411652.496084
GACAAGA200.006929411652.4960852
CCGGACC200.006929411652.4960820
AAGCGGA200.006929411652.4960845
ACCTCGG200.006929411652.4960844
AAACGCA200.006929411652.496083
TCGGAGC200.006929411652.4960847
TTTCTGC200.006929411652.4960828
Produced by FastQC (version 0.11.5)