##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76497 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.437860308247384 34.0 23.0 34.0 12.0 34.0 2 29.161731832620887 34.0 27.0 34.0 12.0 34.0 3 29.51188935513811 34.0 31.0 34.0 11.0 34.0 4 29.83799364680968 34.0 31.0 34.0 11.0 34.0 5 29.766997398590796 34.0 31.0 34.0 11.0 34.0 6 32.16280377008248 37.0 31.0 38.0 11.0 38.0 7 31.524844111533785 37.0 27.0 38.0 11.0 38.0 8 30.696092657228387 36.0 26.0 38.0 11.0 38.0 9 31.367883707857825 37.0 27.0 38.0 12.0 38.0 10-11 31.63307057793116 37.0 27.5 38.0 11.0 38.0 12-13 31.959652012497223 37.0 29.5 38.0 11.0 38.0 14-15 31.565179026628496 37.0 27.0 38.0 11.0 38.0 16-17 31.3430134515079 37.0 27.0 38.0 11.0 38.0 18-19 31.376694510895852 37.0 27.0 38.0 11.0 38.0 20-21 31.48133260127848 37.0 27.0 38.0 11.0 38.0 22-23 31.352386368092866 37.0 27.0 38.0 11.0 38.0 24-25 31.707550622900243 37.0 27.5 38.0 11.0 38.0 26-27 31.7190281971842 37.0 27.5 38.0 11.0 38.0 28-29 31.730277004326965 37.0 27.5 38.0 11.0 38.0 30-31 31.59293828516151 37.0 27.0 38.0 11.0 38.0 32-33 31.457037530883564 37.0 27.0 38.0 11.0 38.0 34-35 31.419225590546034 37.0 27.0 38.0 11.0 38.0 36-37 31.345725976182074 37.0 27.0 38.0 11.0 38.0 38-39 31.654391675490544 37.0 27.0 38.0 11.0 38.0 40-41 31.30696628625959 37.0 27.0 38.0 11.0 38.0 42-43 31.58488568179144 37.0 27.0 38.0 11.0 38.0 44-45 30.82867301985699 37.0 26.0 38.0 11.0 38.0 46-47 31.44567760827222 37.0 27.0 38.0 11.0 38.0 48-49 31.262049492136946 37.0 27.0 38.0 11.0 38.0 50-51 31.262507026419335 37.0 26.5 38.0 11.0 38.0 52-53 31.199432657489837 37.0 27.0 38.0 11.0 38.0 54-55 31.01108540204191 37.0 26.0 38.0 11.0 38.0 56-57 30.97093350066016 37.0 26.5 38.0 11.0 38.0 58-59 30.886100108500987 36.5 26.5 38.0 11.0 38.0 60-61 30.9264546322078 37.0 26.0 38.0 11.0 38.0 62-63 30.942768997477025 37.0 26.0 38.0 11.0 38.0 64-65 30.971541367635332 37.0 26.0 38.0 11.0 38.0 66-67 31.030203798841782 37.0 26.0 38.0 11.0 38.0 68-69 30.55615252885734 36.0 25.0 38.0 11.0 38.0 70-71 30.415388838777993 36.0 24.5 38.0 11.0 38.0 72-73 30.45193929173693 36.0 24.5 38.0 11.0 38.0 74-75 30.067800044446187 36.0 24.0 38.0 11.0 38.0 76 29.33403924336902 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.5402084505195823 1101 2 -2.285941307097918 1101 3 -0.9324383231524394 1101 4 -2.375713059677107 1101 5 -1.2588708264464188 1101 6 -0.7705870178527086 1101 7 -0.24820738363614936 1101 8 -0.8192806560641444 1101 9 -1.5903214380692745 1101 10-11 -0.7330558869559596 1101 12-13 -0.8622578090569668 1101 14-15 -0.3883082720947435 1101 16-17 -0.5627624280876375 1101 18-19 0.14559447433403605 1101 20-21 -0.920313065104363 1101 22-23 -0.652551290464114 1101 24-25 -1.137267305193344 1101 26-27 -0.6680744918671557 1101 28-29 0.24126724620922602 1101 30-31 -1.0369663725815812 1101 32-33 0.05288338415054028 1101 34-35 -0.34487257496099133 1101 36-37 -0.1427361014177464 1101 38-39 -0.9144395142626927 1101 40-41 -0.21272414784051463 1101 42-43 -0.9355358017128133 1101 44-45 -0.013293936782421412 1101 46-47 -0.6655468826347217 1101 48-49 -1.0867871830368117 1101 50-51 -0.8782171379617196 1101 52-53 -0.8033268896275274 1101 54-55 -0.5933537222329086 1101 56-57 -0.3841098206065787 1101 58-59 -0.6186443269169146 1101 60-61 -0.7485025208495948 1101 62-63 -1.4547273782569121 1101 64-65 -0.2638581451206221 1101 66-67 -0.7971227994218992 1101 68-69 -0.3377491522195051 1101 70-71 0.058841243768497975 1101 72-73 -0.7199085609618088 1101 74-75 -1.6832778736565182 1101 76 -2.7019930949405904 1102 1 -0.3064885575668619 1102 2 -0.1819666812678058 1102 3 0.16816629573942166 1102 4 0.4667886748852865 1102 5 0.10419786222441374 1102 6 0.5200265216457538 1102 7 1.1018768665373067 1102 8 1.4298025038129545 1102 9 0.7470757271601869 1102 10-11 -0.052562280057358635 1102 12-13 -4.2030745401502365 1102 14-15 -1.0226625192946663 1102 16-17 -1.3474288965186005 1102 18-19 -0.7819008175680331 1102 20-21 0.1720647721441253 1102 22-23 -0.6268302080971786 1102 24-25 -0.016640880374232836 1102 26-27 -3.855407230493384 1102 28-29 0.3313158139562802 1102 30-31 0.5984286120551516 1102 32-33 -5.672907080712182 1102 34-35 -0.22816130526131673 1102 36-37 0.3488884401621881 1102 38-39 0.7847873664985272 1102 40-41 -0.05606838413747184 1102 42-43 0.2811853797719728 1102 44-45 -4.334088861948441 1102 46-47 -0.26248414103495676 1102 48-49 0.1380616622402222 1102 50-51 -5.219975488640678 1102 52-53 -0.8374729893400854 1102 54-55 0.2356679845764873 1102 56-57 -1.2227459589751 1102 58-59 1.1311326579180516 1102 60-61 1.0911247960301758 1102 62-63 -3.660347360415699 1102 64-65 -0.9381073273983525 1102 66-67 0.4738654055538163 1102 68-69 -5.861438318638374 1102 70-71 -0.8064080376270795 1102 72-73 0.3041275179360028 1102 74-75 -5.437515161792113 1102 76 -1.9309553310393106 1103 1 0.9355681469373636 1103 2 0.6956832435754023 1103 3 0.8840217873685106 1103 4 1.0575988132774512 1103 5 -0.07280934246122683 1103 6 -0.16079035494161076 1103 7 1.1970149995389114 1103 8 1.9720976367627223 1103 9 0.9439346915514122 1103 10-11 1.2413526113540314 1103 12-13 0.011018654272614725 1103 14-15 0.6749998452935309 1103 16-17 0.22459204371879338 1103 18-19 0.7034436078712396 1103 20-21 0.6358728890777314 1103 22-23 0.48313195434515777 1103 24-25 1.1054629914963883 1103 26-27 0.9615730214114571 1103 28-29 1.4428070283863903 1103 30-31 1.3562682430093815 1103 32-33 -3.5857640754771225 1103 34-35 0.5369478016686564 1103 36-37 0.8517780892577171 1103 38-39 1.866423192964092 1103 40-41 1.676204979258575 1103 42-43 1.105292568907931 1103 44-45 -3.481331385143381 1103 46-47 0.584895737677531 1103 48-49 0.5829510003421241 1103 50-51 0.8847127402506345 1103 52-53 1.5723906917877812 1103 54-55 1.4858753136595695 1103 56-57 1.64600177336305 1103 58-59 1.8721008847360707 1103 60-61 1.8920629601311454 1103 62-63 1.2669449217591833 1103 64-65 1.7356992345671252 1103 66-67 1.1633901036308387 1103 68-69 -1.917313506530359 1103 70-71 1.5806235282258214 1103 72-73 2.1412964393064478 1103 74-75 0.7219209031018892 1103 76 1.1307769034498811 1104 1 -2.172041656723497 1104 2 -0.22110530404681228 1104 3 0.39047039792561833 1104 4 0.004658158222714093 1104 5 -0.2367503077583919 1104 6 -0.5984537862229118 1104 7 0.5430461124228394 1104 8 0.4827788405033644 1104 9 -1.477684773945196 1104 10-11 -0.8311997974568506 1104 12-13 -0.8789117627818577 1104 14-15 -0.7563479364730803 1104 16-17 -0.6633217975257253 1104 18-19 0.1866572762664056 1104 20-21 0.3700505250303969 1104 22-23 -0.1655184910743337 1104 24-25 0.06613976320228332 1104 26-27 0.29111696503597173 1104 28-29 -0.9175173965571091 1104 30-31 -0.8181766446385339 1104 32-33 -1.0403024790582052 1104 34-35 -0.11807369369986631 1104 36-37 -0.035947390510042254 1104 38-39 -0.6217875946084845 1104 40-41 0.1513745045876611 1104 42-43 0.23672455933473202 1104 44-45 -2.876502683286361 1104 46-47 -0.11215252718086788 1104 48-49 0.3880411922492293 1104 50-51 0.7862883286032414 1104 52-53 -0.9978348987808445 1104 54-55 -0.17102979647648908 1104 56-57 -0.039334839675994715 1104 58-59 0.43093614600451957 1104 60-61 -0.044714064200725545 1104 62-63 -0.057956465467690066 1104 64-65 0.21414846560910306 1104 66-67 -0.4044200280005903 1104 68-69 -3.766048160610044 1104 70-71 -0.508283088802056 1104 72-73 -0.06760751850058355 1104 74-75 -1.3330363277628017 1104 76 -0.43705538835528657 1105 1 -0.30748711229028913 1105 2 -0.21946503890806568 1105 3 -0.2506485774704075 1105 4 -0.1296638280709601 1105 5 0.35475626453685294 1105 6 -0.4160922538108238 1105 7 0.74749505074438 1105 8 0.008517521550437834 1105 9 0.2497166416260903 1105 10-11 -0.23097510360222984 1105 12-13 -0.9004599034402112 1105 14-15 -0.09323143270946233 1105 16-17 -0.07747751036697892 1105 18-19 0.594173285703544 1105 20-21 -0.41532190503363964 1105 22-23 -0.7499809110271478 1105 24-25 -0.10591955597533698 1105 26-27 -0.2106693511082831 1105 28-29 -0.7181914781209784 1105 30-31 -0.6335120002165588 1105 32-33 0.07035924434365626 1105 34-35 -0.6650684133942839 1105 36-37 -0.05066483798785626 1105 38-39 0.1418232356353073 1105 40-41 0.1811015015542914 1105 42-43 -0.15195087039226607 1105 44-45 0.6406976312850325 1105 46-47 0.18178073874430467 1105 48-49 0.05904445031012173 1105 50-51 -0.18024352175065061 1105 52-53 -0.8559856683572988 1105 54-55 -1.2077560070146305 1105 56-57 -1.4990697009275387 1105 58-59 -0.68665738281247 1105 60-61 -0.43298504498940105 1105 62-63 -0.7139277046543029 1105 64-65 -0.8693253013833733 1105 66-67 -0.288242070467728 1105 68-69 0.6866762523772607 1105 70-71 0.04755333407177886 1105 72-73 0.023651890554578614 1105 74-75 -1.1468437651293861 1105 76 -1.5680069668296142 1106 1 -1.0697140326567443 1106 2 0.11464348822372017 1106 3 -1.0100566112970455 1106 4 -1.107770475945049 1106 5 -0.2806677429801745 1106 6 -1.2378916430520732 1106 7 0.0893790065818223 1106 8 -1.1379941063565404 1106 9 -0.10680203349723882 1106 10-11 -0.6256662006189089 1106 12-13 -0.39867460863166215 1106 14-15 -1.0588292715631127 1106 16-17 -0.5513323377101784 1106 18-19 -0.5059206771811127 1106 20-21 -1.480520401627487 1106 22-23 -0.6715571384173273 1106 24-25 -0.5787877730295996 1106 26-27 -0.7777236146741764 1106 28-29 -0.9684616214143773 1106 30-31 -0.802915335898966 1106 32-33 -0.39833936970379114 1106 34-35 -0.5559305228610434 1106 36-37 -0.4756707106681439 1106 38-39 -0.28724653180655224 1106 40-41 -0.7529952802169078 1106 42-43 0.13955723390653674 1106 44-45 0.6166901142542649 1106 46-47 0.0328848026390709 1106 48-49 -0.2820290756471273 1106 50-51 0.1838509109484363 1106 52-53 -0.7348792553904175 1106 54-55 -0.061455795598142515 1106 56-57 0.5756190470170246 1106 58-59 -0.40173842580048813 1106 60-61 0.25030130690160135 1106 62-63 0.5729174914932216 1106 64-65 0.7018515837470005 1106 66-67 0.3510696514021312 1106 68-69 0.4886729636762972 1106 70-71 0.12489653350800012 1106 72-73 -0.15478362741582075 1106 74-75 -1.440853631232276 1106 76 -1.5220356255094352 1107 1 -0.40716089951537526 1107 2 0.15159673105931404 1107 3 0.33177802465523953 1107 4 0.5293701611955868 1107 5 -0.12225099632813397 1107 6 0.25047619825694767 1107 7 0.2542402890901627 1107 8 -0.20360496608504874 1107 9 -0.8648577033176146 1107 10-11 -0.7765186161772064 1107 12-13 -0.25157007917418994 1107 14-15 -0.7744584136628454 1107 16-17 0.10217102167553449 1107 18-19 0.3549961837357465 1107 20-21 -0.006629913031115819 1107 22-23 -0.6724369775324597 1107 24-25 -0.2137802315514712 1107 26-27 -0.11972964785667628 1107 28-29 -1.2625564155091737 1107 30-31 0.04994585519752093 1107 32-33 0.3913105228872773 1107 34-35 0.28325316926382627 1107 36-37 -0.2046688367250944 1107 38-39 0.2681833324468421 1107 40-41 -0.034440958721408066 1107 42-43 0.3894886750144515 1107 44-45 0.13072183416833738 1107 46-47 -0.18356704616572017 1107 48-49 0.3415026214139836 1107 50-51 0.5723398729668077 1107 52-53 0.0751097751868457 1107 54-55 0.2556976392023529 1107 56-57 0.45908035694892035 1107 58-59 0.3840150364235626 1107 60-61 -0.4229355869235256 1107 62-63 -0.49826949145846555 1107 64-65 0.3921710681841333 1107 66-67 0.25660692458646395 1107 68-69 0.7394385379712851 1107 70-71 -0.20847107927137642 1107 72-73 -0.4637099951657184 1107 74-75 -0.44195057350568945 1107 76 -1.0038903720700674 1108 1 -0.11306420100691383 1108 2 0.24427311785334993 1108 3 -0.4970094732499071 1108 4 -0.32283781601238815 1108 5 0.044248081935649 1108 6 0.05251239735196833 1108 7 0.17187058907340358 1108 8 0.0333021899397572 1108 9 -0.7806235823187855 1108 10-11 0.49891292396021925 1108 12-13 0.26188936443231015 1108 14-15 0.31259328735944436 1108 16-17 -0.9557936171714587 1108 18-19 -0.4076462664067044 1108 20-21 0.4414072078001219 1108 22-23 -0.2710941754543654 1108 24-25 -1.1898988841407103 1108 26-27 -0.976797688748249 1108 28-29 -0.3129481534009102 1108 30-31 -0.3599439554275854 1108 32-33 -0.4389538478182686 1108 34-35 -0.019945674376195655 1108 36-37 -0.13518249517992942 1108 38-39 0.05766087560085609 1108 40-41 -0.18628652600815698 1108 42-43 -0.26448317013386813 1108 44-45 -0.4304479328837836 1108 46-47 -0.2821404498861817 1108 48-49 0.013846345228298418 1108 50-51 -0.5665699644724427 1108 52-53 -0.28517386818503354 1108 54-55 0.5734347767924319 1108 56-57 -0.2257277543297782 1108 58-59 -1.6090195705816335 1108 60-61 0.27976258636288165 1108 62-63 -0.9163501852744531 1108 64-65 -0.3648571704617538 1108 66-67 0.09517739550987869 1108 68-69 0.30138340138673314 1108 70-71 0.4205615166729828 1108 72-73 -0.19834423347642982 1108 74-75 0.7287591633805164 1108 76 -0.43786054133435215 1109 1 -0.3904792889058726 1109 2 -0.9322075102401826 1109 3 0.019268901899437196 1109 4 -0.417457204986615 1109 5 -0.01455034783052156 1109 6 -0.211312487440658 1109 7 -0.30167438447157124 1109 8 -0.006657700826586677 1109 9 0.29530661054689133 1109 10-11 0.23129046278936727 1109 12-13 0.21536905379909754 1109 14-15 0.1708988855843998 1109 16-17 -1.1716367644662178 1109 18-19 -0.047697896377687954 1109 20-21 0.12434158226675507 1109 22-23 0.4568350786845663 1109 24-25 0.16468380915166136 1109 26-27 -0.0031460417095026116 1109 28-29 0.014827490906995422 1109 30-31 -0.24097100782560332 1109 32-33 0.06333963229779016 1109 34-35 -0.8784646195886872 1109 36-37 -0.3940363951628605 1109 38-39 0.04570277694920577 1109 40-41 -0.28531153027509504 1109 42-43 0.1217175467120093 1109 44-45 0.05205761413789034 1109 46-47 -0.9307259593587887 1109 48-49 -0.8005204409126847 1109 50-51 -0.03458925104011712 1109 52-53 0.485446118160759 1109 54-55 -0.041975276117621974 1109 56-57 -0.08624149387577518 1109 58-59 0.456381948448918 1109 60-61 0.2923927280252805 1109 62-63 -0.7781973434925824 1109 64-65 -0.5240246905163701 1109 66-67 -0.5502825805952618 1109 68-69 0.39199186699520183 1109 70-71 0.23944059160044517 1109 72-73 -0.4436399324011582 1109 74-75 0.4303934013050785 1109 76 -0.603844497640889 1110 1 0.36728756223958925 1110 2 -0.013745666481224106 1110 3 -0.4406786208664215 1110 4 -0.4102691468647741 1110 5 -0.7389346222996878 1110 6 -0.5037076983418132 1110 7 -0.6543743161188686 1110 8 0.28066618072480054 1110 9 -1.370757376399947 1110 10-11 -0.9986061261904098 1110 12-13 -0.4789510573291622 1110 14-15 -0.6480105581128193 1110 16-17 -1.3727039485554755 1110 18-19 -0.27253418063672186 1110 20-21 -0.5891062602133452 1110 22-23 -0.6692977019473645 1110 24-25 -0.551674535390049 1110 26-27 -1.718313726316918 1110 28-29 -1.250226640021502 1110 30-31 -1.09066121575011 1110 32-33 0.29796578712767996 1110 34-35 -0.22286300558300098 1110 36-37 -0.7586319015240726 1110 38-39 -0.8027967710410024 1110 40-41 -0.509640681599155 1110 42-43 -0.4302194806070183 1110 44-45 0.37612658421704737 1110 46-47 -0.424717536542218 1110 48-49 -0.3401098837279335 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0.3377998154889461 2116 38-39 0.05976839356658559 2116 40-41 0.11881988637437857 2116 42-43 0.19281367895402823 2116 44-45 1.800618954899523 2116 46-47 0.3554010094836322 2116 48-49 0.5723731556207774 2116 50-51 0.33593141750655064 2116 52-53 0.7328955071604639 2116 54-55 -0.40835764996268153 2116 56-57 0.027865391054412214 2116 58-59 -0.2551983986634454 2116 60-61 -0.5914369714038408 2116 62-63 0.35137369532255747 2116 64-65 0.6455422214674904 2116 66-67 0.47563621253586064 2116 68-69 1.1226244185232765 2116 70-71 -2.2209876817791994 2116 72-73 0.2839318859265596 2116 74-75 -0.8026808587311471 2116 76 -0.4713044329871998 2117 1 -0.9282998912426024 2117 2 -0.006568632988400935 2117 3 -0.07571317695360946 2117 4 0.19525982708525547 2117 5 -1.5129909753034099 2117 6 -1.7429421481025784 2117 7 -2.031833114630299 2117 8 -1.7238526922151252 2117 9 0.40834948165427676 2117 10-11 -0.13071670566941407 2117 12-13 0.3184971085400612 2117 14-15 -0.7204454331792753 2117 16-17 -0.1599181962960401 2117 18-19 0.2541803329198977 2117 20-21 0.014429093322007702 2117 22-23 -0.01751673437692247 2117 24-25 0.24949505525322735 2117 26-27 0.44954911259855024 2117 28-29 0.3572959543432006 2117 30-31 0.02284223985861189 2117 32-33 0.5208525494881258 2117 34-35 0.0753826084520881 2117 36-37 -1.2307212157186527 2117 38-39 0.34911710455708445 2117 40-41 -1.230268007489638 2117 42-43 -0.5094326650833629 2117 44-45 0.30860930617345517 2117 46-47 -0.2196404498861817 2117 48-49 -0.7643523079703591 2117 50-51 0.38334586044338437 2117 52-53 0.4721914516802883 2117 54-55 -1.0715568056991494 2117 56-57 -0.28801731661934227 2117 58-59 -0.5886071126691732 2117 60-61 0.5886851452854422 2117 62-63 0.4491801177558443 2117 64-65 0.020033401928817085 2117 66-67 -1.0453949950625159 2117 68-69 0.06190528690861896 2117 70-71 0.7107551193665884 2117 72-73 1.340165867533674 2117 74-75 0.5566211162424715 2117 76 -0.24930835278216534 2118 1 0.41167124872853833 2118 2 1.1860720596522896 2118 3 1.4306360293955969 2118 4 0.5780881099135371 2118 5 1.0278459655335332 2118 6 1.4453695402091142 2118 7 1.4364208536236696 2118 8 -1.3713274396898747 2118 9 -0.11805744475264746 2118 10-11 0.1367436117909051 2118 12-13 1.3812676713106136 2118 14-15 0.5791938164599735 2118 16-17 1.1133862770084377 2118 18-19 -0.13694520820564193 2118 20-21 -1.3880240091310938 2118 22-23 0.6157444488842607 2118 24-25 1.316450322208496 2118 26-27 1.629419242468682 2118 28-29 0.6119857090329575 2118 30-31 0.635757102773475 2118 32-33 0.475398004033579 2118 34-35 1.8123955954650732 2118 36-37 1.0363783513809146 2118 38-39 0.8913248967648784 2118 40-41 0.2919542147325842 2118 42-43 0.7053580997074 2118 44-45 1.3589700565342078 2118 46-47 0.8676611374154 2118 48-49 0.12244422882617911 2118 50-51 0.9422924693899972 2118 52-53 0.03460126409009945 2118 54-55 0.5604056762633327 2118 56-57 1.2856479070458882 2118 58-59 1.1497767257146663 2118 60-61 0.4276461842464805 2118 62-63 1.3175783861541177 2118 64-65 1.0934100253054417 2118 66-67 -1.2101136097811285 2118 68-69 1.2729442479475814 2118 70-71 0.5197017283131942 2118 72-73 0.47248909985690446 2118 74-75 0.8381506977720541 2118 76 1.0960018925280828 2119 1 0.8277580979760089 2119 2 1.175956096168452 2119 3 0.763377208768901 2119 4 0.99449570922269 2119 5 1.2953515169769076 2119 6 0.6574346771447139 2119 7 -0.5563005872843014 2119 8 1.2169281734362052 2119 9 -0.25195825896434343 2119 10-11 0.5336471400415803 2119 12-13 0.5270855839679065 2119 14-15 0.9664625063193348 2119 16-17 0.22437813489148795 2119 18-19 0.7529635013921769 2119 20-21 0.06303693338062644 2119 22-23 1.3268596721756545 2119 24-25 1.3711258711845673 2119 26-27 1.1734853663275508 2119 28-29 1.182242309082305 2119 30-31 0.6074447415595543 2119 32-33 1.0846380731182066 2119 34-35 1.4197851834246116 2119 36-37 0.5536248354667777 2119 38-39 0.5456550225975931 2119 40-41 0.5636295169778371 2119 42-43 0.1861501056289434 2119 44-45 -1.1231172861748995 2119 46-47 0.23128067849608414 2119 48-49 0.12008794952689428 2119 50-51 1.5027785290988476 2119 52-53 -0.22384926638115843 2119 54-55 -0.05603893266832216 2119 56-57 1.0220653755877045 2119 58-59 1.2644572093070678 2119 60-61 0.9764618763264217 2119 62-63 1.0939045642937657 2119 64-65 1.145013775613858 2119 66-67 1.0018651713686353 2119 68-69 0.3338867596672941 2119 70-71 -0.2194667888345947 2119 72-73 -0.7953865478114857 2119 74-75 -0.1824143157981446 2119 76 0.7612375204580104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 27.0 13 226.0 14 762.0 15 1381.0 16 1860.0 17 2224.0 18 2415.0 19 2302.0 20 2027.0 21 1563.0 22 1309.0 23 1064.0 24 928.0 25 963.0 26 1016.0 27 1104.0 28 1326.0 29 1515.0 30 1870.0 31 2233.0 32 2862.0 33 3863.0 34 5310.0 35 7734.0 36 13108.0 37 15502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.098980962161306 28.060404981962765 10.164046659820418 28.676567396055507 2 16.544439651228153 23.027046812293293 32.227407610755975 28.201105925722576 3 15.335241904911303 30.056080630612964 27.831156777389964 26.777520687085772 4 10.546818829496582 27.1213250192818 33.90067584349713 28.431180307724485 5 10.26706929683517 40.920558976169005 31.973802894231145 16.838568832764683 6 21.330248245029217 38.81459403636744 17.16668627527877 22.688471443324577 7 23.07280024053231 33.25882060734408 20.73806815953567 22.930310992587945 8 21.361622024393114 36.354366837915215 18.72099559459848 23.563015543093194 9 21.169457625789246 20.26746146907722 19.873981986221683 38.68909891891185 10-11 21.53352419049113 27.53768121625685 26.061806345346877 24.866988247905148 12-13 20.61976286651764 25.324522530295308 25.312757363033846 28.74295724015321 14-15 19.988365556819222 26.81085532765991 22.718537981881642 30.482241133639228 16-17 21.123704197550232 28.67105899577761 23.995712250153602 26.209524556518556 18-19 19.966796083506544 27.77690628390656 24.47808410787351 27.778213524713387 20-21 19.538674719270034 27.924624495078238 24.66044420042616 27.876256585225562 22-23 21.72438134828817 27.66121547250219 24.344745545576952 26.269657633632693 24-25 20.175954612599188 29.20049152254337 24.24735610546819 26.376197759389257 26-27 19.317097402512516 29.735153012536443 25.411454043949437 25.536295541001607 28-29 20.69950455573421 28.035739963658706 26.863798580336486 24.4009569002706 30-31 20.352432121521105 27.811548165287526 27.127861223315946 24.70815848987542 32-33 19.16088212609645 29.472397610363803 26.082722198256143 25.28399806528361 34-35 19.879864569852412 28.642953318430784 26.279461939683905 25.197720172032888 36-37 20.30537145247526 27.46839745349491 26.61150110461848 25.61472998941135 38-39 20.195563224701623 26.753336732159433 27.692589251866085 25.35851079127286 40-41 19.674627763180254 26.68078486738042 27.076225211446204 26.568362157993125 42-43 18.441899682340484 26.929160620677933 28.002405323084567 26.626534373897016 44-45 18.421637449834634 26.761833797403824 28.499156829679595 26.31737192308195 46-47 18.80335176542871 27.43963815574467 27.785403349150943 25.971606729675674 48-49 18.426212792658536 28.160581460710876 27.184072578009594 26.229133168620994 50-51 18.430788135482437 27.933121560322625 26.489274089179972 27.14681621501497 52-53 17.854948560074252 27.565786893603672 26.946154751166713 27.633109795155363 54-55 17.62422055766893 27.84815090787874 26.704968822306757 27.822659712145576 56-57 18.4425533027439 27.75729767180412 26.207563695308313 27.592585330143667 58-59 18.38045936441952 27.680170464201208 25.953305358380067 27.9860648129992 60-61 17.90135560871668 26.711505026340905 26.285998143718054 29.101141221224363 62-63 18.060838987149822 26.981450252951095 26.302992274206833 28.65471848569225 64-65 18.445167784357558 27.759912153417783 25.106866935958273 28.68805312626639 66-67 18.10267069296835 28.65602572649908 24.382001908571578 28.859301671960992 68-69 18.211171679935163 28.551446461952757 24.65194713518177 28.58543472293031 70-71 18.973293070316483 28.30764605147914 24.393113455429624 28.32594742277475 72-73 19.130161967135965 27.348784919670056 24.7388786488359 28.78217446435808 74-75 19.6452148450266 26.908898388172087 24.71796279592664 28.727923970874674 76 19.73018549747049 25.774866988247908 25.62453429546257 28.870413218819042 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.5 14 3.5 15 8.0 16 12.0 17 13.0 18 16.0 19 19.0 20 26.0 21 33.0 22 40.5 23 70.0 24 98.5 25 105.0 26 133.5 27 218.0 28 323.5 29 373.0 30 430.5 31 580.5 32 877.0 33 1081.0 34 1261.5 35 1619.0 36 1966.0 37 2136.0 38 2498.0 39 3191.5 40 3803.5 41 4414.5 42 4745.0 43 4937.0 44 5330.0 45 5507.0 46 5483.0 47 5562.5 48 5517.5 49 5036.0 50 4679.0 51 4396.0 52 3802.0 53 3236.0 54 2981.0 55 2785.0 56 2275.5 57 1701.0 58 1440.0 59 1263.0 60 959.5 61 711.0 62 589.0 63 518.5 64 374.5 65 269.5 66 198.5 67 159.0 68 131.5 69 88.0 70 65.0 71 58.0 72 46.0 73 29.5 74 21.5 75 18.0 76 17.0 77 14.5 78 14.0 79 15.0 80 11.5 81 5.5 82 5.0 83 7.0 84 6.5 85 4.0 86 2.0 87 2.0 88 4.5 89 6.0 90 3.0 91 3.0 92 5.0 93 6.0 94 5.5 95 2.5 96 1.0 97 2.0 98 3.5 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7098317581081611 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 76497.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.08359804959672 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57952879265672 53.09489261016772 2 7.947857874545564 10.345503745244912 3 3.8885652881274226 7.592454606062983 4 2.1953522003735912 5.715256807456502 5 1.183039749332155 3.8498241761114813 6 0.8235081446964067 3.215812384799404 7 0.6286781689999398 2.864164607762396 8 0.37359149978910156 1.9451743205615908 9 0.31534336272520935 1.8471312600494136 >10 1.0645349187538915 9.5297854817836 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.00653620403414513 0.0 10 0.0 0.0 0.0 0.007843444840974156 0.0 11 0.0 0.0 0.0 0.009150685647803181 0.0 12 0.0 0.0 0.0 0.01307240806829026 0.0 13 0.0 0.0 0.0 0.014379648875119286 0.0 14 0.0 0.0 0.0 0.018301371295606363 0.0 15 0.0 0.0 0.0 0.018301371295606363 0.0 16 0.0 0.0 0.0 0.01960861210243539 0.0 17 0.0 0.0 0.0 0.024837575329751494 0.0 18 0.0 0.0 0.0 0.033988260977554675 0.0 19 0.0 0.0 0.0 0.03921722420487078 0.0 20 0.0 0.0 0.0 0.0405244650116998 0.0 21 0.0 0.0 0.001307240806829026 0.04444618743218688 0.0 22 0.0 0.0 0.001307240806829026 0.053596873079990066 0.0 23 0.0 0.0 0.001307240806829026 0.057518595500477145 0.0 24 0.0 0.0 0.001307240806829026 0.06144031792096422 0.0 25 0.0 0.0 0.001307240806829026 0.06274755872779325 0.0 26 0.0 0.0 0.001307240806829026 0.06405479953462227 0.0 27 0.0 0.0 0.001307240806829026 0.06928376276193837 0.0 28 0.0 0.0 0.001307240806829026 0.08889237486437376 0.0 29 0.0 0.0 0.001307240806829026 0.100657542125835 0.0 30 0.0 0.0 0.001307240806829026 0.12157339503509941 0.0 31 0.0 0.0 0.001307240806829026 0.13987476633070578 0.0 32 0.0 0.0 0.001307240806829026 0.16863406408094436 0.0 33 0.0 0.0 0.001307240806829026 0.21961645554727635 0.0 34 0.0 0.0 0.001307240806829026 0.2719060878204374 0.0 35 0.0 0.0 0.001307240806829026 0.3333464057414016 0.0 36 0.0 0.0 0.001307240806829026 0.4588415231969881 0.0 37 0.0 0.0 0.001307240806829026 0.6470841993803679 0.0 38 0.0 0.0 0.001307240806829026 0.9202975280076343 0.0 39 0.0 0.0 0.001307240806829026 1.2444932481012327 0.0 40 0.0 0.0 0.001307240806829026 1.5896048211040956 0.0 41 0.0 0.0 0.001307240806829026 2.009229120096213 0.0 42 0.0 0.0 0.001307240806829026 2.404015843758579 0.0 43 0.0 0.0 0.001307240806829026 2.80533877145509 0.0 44 0.0 0.0 0.001307240806829026 3.224963070447207 0.0 45 0.0 0.0 0.001307240806829026 3.666810463155418 0.0 46 0.0 0.0 0.001307240806829026 4.0733623540792445 0.0 47 0.001307240806829026 0.0 0.001307240806829026 4.52305319162843 0.0 48 0.001307240806829026 0.0 0.001307240806829026 4.943984731427376 0.0 49 0.001307240806829026 0.0 0.001307240806829026 5.359687307999007 0.0 50 0.001307240806829026 0.0 0.001307240806829026 5.78454057021844 0.0 51 0.001307240806829026 0.0 0.001307240806829026 6.196321424369583 0.0 52 0.001307240806829026 0.0 0.001307240806829026 6.703530857419246 0.0 53 0.001307240806829026 0.0 0.001307240806829026 7.08655241382015 0.0 54 0.001307240806829026 0.0 0.001307240806829026 7.538857732982993 0.0 55 0.001307240806829026 0.0 0.001307240806829026 7.934951697452187 0.0 56 0.001307240806829026 0.0 0.001307240806829026 8.371570126933083 0.0 57 0.001307240806829026 0.0 0.001307240806829026 8.823875446095926 0.0 58 0.001307240806829026 0.0 0.001307240806829026 9.281409728486084 0.0 59 0.001307240806829026 0.0 0.001307240806829026 9.669660248114305 0.0 60 0.001307240806829026 0.0 0.001307240806829026 10.035687674026432 0.0 61 0.001307240806829026 0.0 0.001307240806829026 10.422630952847824 0.0 62 0.001307240806829026 0.0 0.001307240806829026 10.79127286037361 0.0 63 0.001307240806829026 0.0 0.001307240806829026 11.22004784501353 0.0 64 0.001307240806829026 0.0 0.001307240806829026 11.574310103664196 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACTAG 15 0.0021719 70.36292 1 GTTAGAG 15 0.0022176043 69.99477 3 GTATGGA 15 0.0022176043 69.99477 20 TCGCTCA 15 0.0022176043 69.99477 59 CTGGAGA 15 0.0022176043 69.99477 4 CTTGGTT 25 2.8169397E-6 69.99476 30 TGCTTGG 30 8.3095E-6 58.32897 28 GCTTGGT 30 8.3095E-6 58.32897 29 CCTTGCT 30 8.3095E-6 58.32897 15 CCTTGCG 30 8.3095E-6 58.32897 54 GATACTA 20 0.0069294116 52.49608 65 GAAGCGG 20 0.0069294116 52.49608 44 TTAGAGT 20 0.0069294116 52.49608 4 GACAAGA 20 0.0069294116 52.49608 52 CCGGACC 20 0.0069294116 52.49608 20 AAGCGGA 20 0.0069294116 52.49608 45 ACCTCGG 20 0.0069294116 52.49608 44 AAACGCA 20 0.0069294116 52.49608 3 TCGGAGC 20 0.0069294116 52.49608 47 TTTCTGC 20 0.0069294116 52.49608 28 >>END_MODULE