FastQCFastQC Report
Wed 25 May 2016
SRR1780430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76497
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT37134.853785115756174No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT33314.3544191275474855Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC11671.5255500215694733No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC5730.7490489823130319No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG3500.4575342823901591No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT2880.3764853523667595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1950.25491195733166006No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1600.20915852909264418No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1440.18824267618337975No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1270.1660195824672863No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1220.15948337843314117No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1070.13987476633070578No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC840.10980822777363819No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC820.10719374615998013No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA150.00221694770.09
GCCGAAT150.00221694770.011
CTTATAA150.00221694770.01
CCGAATA150.00221694770.012
TAGTGGC150.00221694770.011
TTTACAC2200.065.227272
TTACACA2550.057.647063
ATCTAGT252.3768976E-455.99999637
TTAATAC452.6591806E-854.4444432
CTTTACA2700.054.4444431
AACCGTT200.00692736352.50000424
AGCCGAA200.00692736352.50000410
CATATAC200.00692736352.50000457
ACATTAT200.00692736352.50000424
GCCTGAC200.00692736352.50000452
CTAAAAG200.00692736352.50000413
CACTACA200.00692736352.50000449
TATTAAG200.00692736352.50000462
CGAATAC200.00692736352.50000413
ACCGTTT200.00692736352.50000425
Produced by FastQC (version 0.11.5)