FastQCFastQC Report
Wed 25 May 2016
SRR1780429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557904
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGT301.3118432E-436.6113244
GGGTCTA250.002351542535.2005041
ACGATTC300.005734435729.34165223
GGAATAT407.0267555E-427.5003911
CGGTGGT1450.027.2813543
CTATACA1650.025.3336941
GCCATAG701.1302964E-625.1432151
TCTAGCA801.3264253E-724.7126433
GTAGAGC450.001391317224.46235838
GTATCGA450.001409620324.4075496
ATCGTCC551.5948708E-423.99388129
ACGAGAG1301.8189894E-1223.70967124
GGAGATC852.3313805E-723.31118838
CGAGTAT852.3639222E-723.27773541
ACGCCTT1105.2314135E-922.00228910
GTACAAG500.002578493722.0003151
TCTGTAC1105.2368705E-922.0003151
CAATGTT500.00258522121.99044626
TCGGTGG2500.021.98452842
CTGTACC1105.313268E-921.9727042
Produced by FastQC (version 0.11.5)