FastQCFastQC Report
Wed 25 May 2016
SRR1780429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557904
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT350156.276169376810348No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT282065.0557085089907945RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC83951.5047391665949696No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC47420.8499670194155268No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG42300.7581949582723909No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT25640.45957727494335937No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC21780.39038974447216723No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC13780.24699589893601767No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG11870.21276061831426193No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG9610.1722518569502997No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC8500.15235596088215894No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC6930.12421491869568958No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC6710.12027158794344547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT29850.042.45303744
GCCGTCT32200.039.3547643
GATCATA301.2918892E-436.7068181
CTTTACA10100.036.4160731
CTATACA6300.036.0076451
CTTATAA1350.035.8911131
AACTCGG250.002352533235.1974930
TTTACAC10650.034.2947852
TACCCGC401.8180963E-533.00060711
CAAAGGC551.3712634E-732.00058714
CCAACGT551.3723366E-731.99771922
TCGTTGT502.359755E-630.79780434
CATCGTT502.359755E-630.79780432
TTACACA12100.030.3669153
CTTATAC51450.030.221591
TTATACA51600.029.8055442
TTAATAC2450.029.635852
TCTTTAT2250.029.3654541
CTACCCG454.0651536E-529.33387210
CGGCTAC300.005741881729.33387212
Produced by FastQC (version 0.11.5)