FastQCFastQC Report
Wed 25 May 2016
SRR1780428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48550
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGC200.00691582252.49304640
ACAGCCA200.00691582252.49304632
AAAATTA200.00691582252.49304640
GGGATAT200.00691582252.49304613
CGACGTT1252.9956245E-622.39703470
CAAAACA950.006746097518.590931
TACACAA1000.00956856117.4976835
ACGACGT1602.5280127E-517.49768369
GACGACG2000.002718594412.24837868
CGACGAC2459.431847E-411.42705768
TGCCGAC4451.2798046E-711.00977870
CTGCCGA5006.361188E-79.79870269
GCTGCCG5401.8166666E-69.07287368
CGCTGCC5855.3629865E-68.37495967
ACGCTGC6109.408606E-68.03172466
GACGCTG6301.4484327E-57.776747765
CTGACGC7301.29540385E-57.19082963
TGACGCT6854.4005406E-57.15233764
ATCTGAC8259.684289E-66.7869861
TCTGACG7752.9968318E-56.77329762
Produced by FastQC (version 0.11.5)