##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780428_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48550 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.90661174047374 33.0 23.0 34.0 12.0 34.0 2 28.86368692070031 34.0 23.0 34.0 12.0 34.0 3 28.659402677651904 34.0 23.0 34.0 11.0 34.0 4 29.346549948506695 34.0 27.0 34.0 11.0 34.0 5 29.198661174047373 34.0 27.0 34.0 11.0 34.0 6 31.770628218331616 37.0 27.0 38.0 11.0 38.0 7 31.07847579814624 36.0 27.0 38.0 11.0 38.0 8 30.64941297631308 36.0 26.0 38.0 11.0 38.0 9 31.060926879505665 36.0 26.0 38.0 12.0 38.0 10-11 31.131462409886716 37.0 26.5 38.0 11.0 38.0 12-13 31.383882595262616 37.0 27.0 38.0 11.0 38.0 14-15 31.01773429454171 37.0 26.0 38.0 11.0 38.0 16-17 30.812677651905254 37.0 26.0 38.0 11.0 38.0 18-19 30.85974253347065 37.0 26.0 38.0 11.0 38.0 20-21 31.002481977342946 37.0 26.5 38.0 11.0 38.0 22-23 30.826240988671472 37.0 26.0 38.0 11.0 38.0 24-25 31.21136972193615 37.0 26.0 38.0 11.0 38.0 26-27 31.175149330587026 37.0 26.0 38.0 11.0 38.0 28-29 31.12676622039135 37.0 26.0 38.0 11.0 38.0 30-31 31.019248197734296 37.0 26.0 38.0 11.0 38.0 32-33 30.92183316168898 37.0 26.0 38.0 11.0 38.0 34-35 30.835478887744593 37.0 26.0 38.0 11.0 38.0 36-37 30.77697219361483 37.0 26.0 38.0 11.0 38.0 38-39 31.087651905252315 37.0 26.0 38.0 11.0 38.0 40-41 30.75152420185376 37.0 26.0 38.0 11.0 38.0 42-43 31.09020597322348 37.0 26.0 38.0 11.0 38.0 44-45 30.260803295571577 36.0 25.0 38.0 11.0 38.0 46-47 30.89315139031926 37.0 26.0 38.0 11.0 38.0 48-49 30.711606591143152 37.0 26.0 38.0 11.0 38.0 50-51 30.673450051493305 36.5 25.5 38.0 11.0 38.0 52-53 30.69565396498455 37.0 26.0 38.0 11.0 38.0 54-55 30.56200823892894 36.0 25.5 38.0 11.0 38.0 56-57 30.43552008238929 36.5 24.5 38.0 11.0 38.0 58-59 30.368352214212152 36.5 25.0 38.0 11.0 38.0 60-61 30.459938208032955 36.5 25.0 38.0 11.0 38.0 62-63 30.428115345005146 36.0 25.0 38.0 11.0 38.0 64-65 30.432574665293515 36.5 25.0 38.0 11.0 38.0 66-67 30.46114315139032 36.0 25.0 38.0 11.0 38.0 68-69 30.054562306900102 36.0 23.5 38.0 11.0 38.0 70-71 29.84316168898043 36.0 23.5 38.0 11.0 38.0 72-73 29.871637487126673 36.0 23.5 38.0 11.0 38.0 74-75 29.387806385169927 35.0 22.5 38.0 11.0 38.0 76 28.467723995880537 34.0 21.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.707646048887767 1101 2 -1.2146197941651025 1101 3 -0.7396041784238925 1101 4 -2.543003698801474 1101 5 -1.2761910845980573 1101 6 -2.1849053646629457 1101 7 -1.7343500991081413 1101 8 -0.5885493421973429 1101 9 -0.7022986318348501 1101 10-11 -0.9563651680628062 1101 12-13 -1.2124323541160145 1101 14-15 -0.8071292453105876 1101 16-17 -1.6239354075548391 1101 18-19 -0.6290576416025537 1101 20-21 -0.9997460387997243 1101 22-23 0.7416298929263618 1101 24-25 -1.6511266192896592 1101 26-27 -0.8521230240990292 1101 28-29 -0.14459162710578966 1101 30-31 -1.2327435103431412 1101 32-33 -0.07340218833486745 1101 34-35 -1.3349566380648277 1101 36-37 -0.3303672632671599 1101 38-39 -0.17496075609191308 1101 40-41 0.19898638563246607 1101 42-43 -0.9780556621023955 1101 44-45 0.04028781663182812 1101 46-47 0.35804518470619584 1101 48-49 -0.9460814325843323 1101 50-51 -1.312086005559845 1101 52-53 -0.7133995314639705 1101 54-55 -2.049530181643064 1101 56-57 -1.060757847503691 1101 58-59 -0.5890714555067937 1101 60-61 -0.052936816093534844 1101 62-63 -1.365781067371099 1101 64-65 -0.3221903681366882 1101 66-67 -1.3050342349218553 1101 68-69 0.3888508000494646 1101 70-71 0.7091473142584555 1101 72-73 -0.9629559160696353 1101 74-75 -0.9400881001633756 1101 76 -2.834690918056964 1102 1 1.4510449668413727 1102 2 1.1405521599804587 1102 3 0.632563243596902 1102 4 1.4692596976261463 1102 5 1.1368987581105543 1102 6 2.3365555790853882 1102 7 1.9793929027580326 1102 8 2.0330182397807945 1102 9 2.425533946144359 1102 10-11 0.8917086789113355 1102 12-13 -2.209499802796369 1102 14-15 0.34592993890695567 1102 16-17 0.2692264159588902 1102 18-19 1.7830657921484487 1102 20-21 1.4430692104671365 1102 22-23 -0.3252189473828899 1102 24-25 1.2336374436268933 1102 26-27 -3.1761699449201437 1102 28-29 1.422223888756644 1102 30-31 1.1368579292729635 1102 32-33 -5.609959106490024 1102 34-35 0.6633104907008338 1102 36-37 1.7231517983164188 1102 38-39 1.4428411633962241 1102 40-41 1.191121816184321 1102 42-43 1.97052471646332 1102 44-45 -3.854007038073579 1102 46-47 0.8644434784945183 1102 48-49 0.6829841499269982 1102 50-51 -4.538925515023987 1102 52-53 -0.8661587956601835 1102 54-55 1.5427785360322233 1102 56-57 0.25109232578342855 1102 58-59 1.5814497388413784 1102 60-61 0.716391363058694 1102 62-63 -1.9009383587600617 1102 64-65 0.4905262407676041 1102 66-67 1.1993312676108019 1102 68-69 -5.863181991206201 1102 70-71 0.6903856507020691 1102 72-73 1.4714948970468704 1102 74-75 -4.847279515785878 1102 76 -1.2987005154976465 1103 1 -0.42501314343087415 1103 2 -0.08737051217694258 1103 3 -0.3850528625572984 1103 4 0.048989736518848304 1103 5 -0.038690351932913813 1103 6 -1.3616828102991931 1103 7 0.6039067439842931 1103 8 1.2802156774380826 1103 9 0.3828663643552943 1103 10-11 -0.0161213371033071 1103 12-13 -0.5805819350315566 1103 14-15 -0.5622432480067943 1103 16-17 -0.4973601774979848 1103 18-19 0.3841010243607528 1103 20-21 -0.044233466265875876 1103 22-23 0.7267714438319359 1103 24-25 0.7524546479279692 1103 26-27 -0.6325872407334572 1103 28-29 0.8298823193217366 1103 30-31 -0.2324797508917591 1103 32-33 -3.4248470039963124 1103 34-35 -0.005872762559288702 1103 36-37 -0.33101200859275437 1103 38-39 0.332483121758159 1103 40-41 1.2143430470239451 1103 42-43 0.6341886377627937 1103 44-45 -3.8175165324684563 1103 46-47 -0.5938040621140388 1103 48-49 0.4240903041251194 1103 50-51 0.42181001460309986 1103 52-53 0.19595513707831458 1103 54-55 0.5850457517723271 1103 56-57 0.825503215739964 1103 58-59 0.14708117329573867 1103 60-61 0.717592461205566 1103 62-63 0.5108381225943184 1103 64-65 0.9296706013258316 1103 66-67 -0.17069275435213527 1103 68-69 -2.4079371959648377 1103 70-71 0.3804737312328399 1103 72-73 0.558174146646504 1103 74-75 -0.5295318607870207 1103 76 0.8798627423380907 1104 1 -1.9053618431944699 1104 2 0.4866543105180945 1104 3 -0.9122396237866113 1104 4 -0.45972775757098816 1104 5 -0.24347758597546942 1104 6 -1.5270196897318158 1104 7 0.5100828669157345 1104 8 1.2765218240101888 1104 9 0.03978125797231513 1104 10-11 0.06957606242387726 1104 12-13 -1.1571924551261183 1104 14-15 0.35265036901448354 1104 16-17 -0.7760244800984566 1104 18-19 0.4470442867721012 1104 20-21 0.10883981978613377 1104 22-23 -0.038816617633784745 1104 24-25 -0.8412953520720272 1104 26-27 -0.6731457513717558 1104 28-29 -0.6919114159028545 1104 30-31 -1.0079673395442441 1104 32-33 -1.4142825831925308 1104 34-35 -0.3996375379729997 1104 36-37 0.044948393298497535 1104 38-39 -0.43081475058226815 1104 40-41 0.06836195955349567 1104 42-43 0.2889610963916347 1104 44-45 -2.913482844525195 1104 46-47 0.1502857724013289 1104 48-49 1.14210153343954 1104 50-51 0.5479920477000277 1104 52-53 0.2746342150925045 1104 54-55 -0.4841031843978847 1104 56-57 -0.44525624052481305 1104 58-59 0.7369156886621688 1104 60-61 0.7216556999762531 1104 62-63 0.038689777440655604 1104 64-65 0.0513461332407239 1104 66-67 0.31985098087245234 1104 68-69 -3.788921238518025 1104 70-71 -0.29550144607212303 1104 72-73 1.0360110260791267 1104 74-75 -0.899138834782292 1104 76 -0.24292094560517086 1105 1 -0.752147002479628 1105 2 0.34227785364163665 1105 3 0.6246679131209234 1105 4 -0.01979963014285957 1105 5 -0.28572700322488487 1105 6 -0.2465709717831004 1105 7 1.7297312740641395 1105 8 0.34533495532332026 1105 9 1.5558068989979539 1105 10-11 1.195255938103756 1105 12-13 0.021833380149718806 1105 14-15 0.10672962309373446 1105 16-17 0.419294090220113 1105 18-19 0.30864102336883903 1105 20-21 0.43382039476670897 1105 22-23 0.8048848198176515 1105 24-25 0.7058346013079237 1105 26-27 1.1373874654114573 1105 28-29 -0.018081773323206818 1105 30-31 0.23992018959328476 1105 32-33 1.2559048682449188 1105 34-35 1.3020427262072616 1105 36-37 0.42837399547410016 1105 38-39 1.1603857156181974 1105 40-41 -0.18086103966950162 1105 42-43 0.7660638547443916 1105 44-45 1.1553545680622186 1105 46-47 1.2938366659522238 1105 48-49 0.27674119932920505 1105 50-51 -0.09720997250198948 1105 52-53 0.11940402861231547 1105 54-55 -0.3273432776379792 1105 56-57 0.11692810290952949 1105 58-59 1.2656647174111981 1105 60-61 0.8834903930609492 1105 62-63 0.3535450610459385 1105 64-65 0.4146182777628695 1105 66-67 -0.33904567802420615 1105 68-69 0.6218450784982927 1105 70-71 0.5607048476341703 1105 72-73 1.08408794915605 1105 74-75 -0.08535192724538376 1105 76 -1.2561785213627488 1106 1 0.02491593458330854 1106 2 -0.17951235614857453 1106 3 -0.19401740156439118 1106 4 -0.1454499797932094 1106 5 -0.24981091930879984 1106 6 -0.959186356398483 1106 7 -0.3876393553064865 1106 8 -0.3397559537675896 1106 9 -0.3198854086943541 1106 10-11 0.4173538402016561 1106 12-13 0.21811310042943788 1106 14-15 -0.3726829643188516 1106 16-17 -0.2265800356540133 1106 18-19 -0.32528904656123103 1106 20-21 -0.6553824024360893 1106 22-23 0.23187782681065983 1106 24-25 -0.12887868540536473 1106 26-27 -0.7073957513717559 1106 28-29 -1.16555030479174 1106 30-31 -0.07188400621090807 1106 32-33 0.7249118612519112 1106 34-35 -0.4159153157507802 1106 36-37 -0.06491271781261432 1106 38-39 -0.7044814172489353 1106 40-41 -0.37274915155761335 1106 42-43 -0.2207333480528071 1106 44-45 0.21076715547480518 1106 46-47 -0.3134920053764496 1106 48-49 0.32062931121732063 1106 50-51 0.10388093658891862 1106 52-53 -0.4754491182408316 1106 54-55 -0.4607698510645548 1106 56-57 0.9223826483640778 1106 58-59 -0.26219542244894356 1106 60-61 0.751294588865143 1106 62-63 0.5743842218851007 1106 64-65 -0.028570533425941846 1106 66-67 1.1060732030946738 1106 68-69 0.05549542814863884 1106 70-71 0.26299855392787563 1106 72-73 -0.8509889739208738 1106 74-75 -1.3946666125600728 1106 76 -0.17108761227184033 1107 1 -0.07040321435286145 1107 2 -0.6902073916095652 1107 3 -0.46306704695446044 1107 4 -1.1850528166726413 1107 5 -1.3365626923584486 1107 6 -0.5957253634906827 1107 7 -0.5095684333206698 1107 8 -0.01056446440588843 1107 9 -1.39727547961634 1107 10-11 -1.0019369399401867 1107 12-13 -1.4919294527620508 1107 14-15 -1.5835198437514748 1107 16-17 -0.48672187962564806 1107 18-19 -0.11235287634846713 1107 20-21 -0.33146750881906684 1107 22-23 -1.3867037334730306 1107 24-25 -1.7262687563273467 1107 26-27 -1.0049276662653739 1107 28-29 -2.152387184224363 1107 30-31 -0.9736145026648089 1107 32-33 -0.2475420394573078 1107 34-35 -0.6689933299351765 1107 36-37 -0.6820758383799905 1107 38-39 -0.7455310626390066 1107 40-41 -0.009061208295204182 1107 42-43 -0.3729035608187665 1107 44-45 -0.5267363906244888 1107 46-47 -1.0689813670785746 1107 48-49 -0.7000231710521838 1107 50-51 0.46436320609246096 1107 52-53 -0.13028599767345383 1107 54-55 -0.5213372269510756 1107 56-57 0.2226663363073378 1107 58-59 0.18254216620353958 1107 60-61 -0.7327621487235092 1107 62-63 -1.2090200334340508 1107 64-65 -0.8646556398089231 1107 66-67 -0.7451608394585172 1107 68-69 -0.3370152101492323 1107 70-71 -1.0663347794054552 1107 72-73 -0.7361520944882507 1107 74-75 -0.8167659033402117 1107 76 -2.3470876122718387 1108 1 -0.7079412082738337 1108 2 -0.23894092757714347 1108 3 -0.07573168727867596 1108 4 0.20197859163536336 1108 5 0.8196176521197707 1108 6 1.433099357887233 1108 7 1.4789320732649394 1108 8 1.3648154748038372 1108 9 0.5552574484485042 1108 10-11 0.9187824116302252 1108 12-13 0.5885416718580103 1108 14-15 0.4228884642525763 1108 16-17 -0.46729432136829985 1108 18-19 0.670853810581626 1108 20-21 0.9080461689924846 1108 22-23 0.21902068395351648 1108 24-25 -0.11659297111964761 1108 26-27 0.22774710577110113 1108 28-29 0.8040211237796875 1108 30-31 0.9655445652176624 1108 32-33 1.6953404326804815 1108 34-35 1.3457989699635107 1108 36-37 0.8936587107588174 1108 38-39 1.2346614398939195 1108 40-41 0.885822277013812 1108 42-43 0.7025523662329043 1108 44-45 0.8141957269033746 1108 46-47 1.2582222803378365 1108 48-49 0.7529150255030324 1108 50-51 1.1047380794460615 1108 52-53 1.1332651674734535 1108 54-55 1.3813730060783058 1108 56-57 1.890954076935504 1108 58-59 -0.07062399387751128 1108 60-61 0.9721517317222848 1108 62-63 1.3286699361708152 1108 64-65 1.1820008951454852 1108 66-67 0.9527874888089585 1108 68-69 1.6500668567200698 1108 70-71 2.0491414110707353 1108 72-73 0.28958245465055654 1108 74-75 1.1757619588684989 1108 76 1.1671981020138773 1109 1 -0.48175073208335917 1109 2 0.3130431994069838 1109 3 -0.44001740156438984 1109 4 -0.8902833131265417 1109 5 -0.486533141531023 1109 6 -0.3603530230651515 1109 7 -1.1913060219731548 1109 8 0.17929960178796733 1109 9 0.14394792463897943 1109 10-11 -0.11792393757612274 1109 12-13 -0.6155257884594469 1109 14-15 -0.5674885198744093 1109 16-17 -1.1371355912095709 1109 18-19 -0.23698349100567384 1109 20-21 -0.4432435135471984 1109 22-23 -0.306177728744899 1109 24-25 0.7316213145946371 1109 26-27 -1.0064790847050915 1109 28-29 -0.129411415902851 1109 30-31 -0.17810622843312984 1109 32-33 0.15168963902969068 1109 34-35 -0.1253319824174426 1109 36-37 -1.198107162257056 1109 38-39 -0.22942586169337886 1109 40-41 -0.8378880404465043 1109 42-43 -0.41937223694169745 1109 44-45 -0.19820506674741623 1109 46-47 -1.110130894265339 1109 48-49 -0.9342873554493494 1109 50-51 -0.3999246189666401 1109 52-53 0.05935643731472595 1109 54-55 -0.0014642955089954057 1109 56-57 -0.9695617960803666 1109 58-59 -1.4401676446711633 1109 60-61 -0.35473318891263617 1109 62-63 -1.7217268892260122 1109 64-65 -1.4972649778703868 1109 66-67 -1.2218156857942155 1109 68-69 0.013162094815307057 1109 70-71 -0.9274458905165659 1109 72-73 -0.5195445294764305 1109 74-75 -0.47552772367118123 1109 76 -1.3123653900496173 1110 1 0.4806905824706327 1110 2 -0.6703292575570252 1110 3 -1.1477638804376298 1110 4 -0.6722668812016614 1110 5 0.11509048914190245 1110 6 -0.5959750887928514 1110 7 -0.37851259474310694 1110 8 1.3235679898943822 1110 9 -0.8082234368633685 1110 10-11 -1.2125053147279203 1110 12-13 -0.7825347868945052 1110 14-15 -0.49828859812166826 1110 16-17 -0.09038285255542178 1110 18-19 0.30561236188947305 1110 20-21 -0.5781711348304555 1110 22-23 -0.2902348492456781 1110 24-25 0.7747551174115372 1110 26-27 -0.7554520893999239 1110 28-29 -0.30659255831286814 1110 30-31 -0.08616569635175253 1110 32-33 0.5587710161814883 1110 34-35 -0.3596336256099306 1110 36-37 0.07776333852541484 1110 38-39 -0.426706769361612 1110 40-41 0.24246211604801715 1110 42-43 0.39777369420071196 1110 44-45 1.232203775193117 1110 46-47 0.22814179744045404 1110 48-49 1.4907419872736547 1110 50-51 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-0.5285332019296405 2116 36-37 1.560615737471938 2116 38-39 1.820201509580336 2116 40-41 1.3657874338082365 2116 42-43 1.469770716987842 2116 44-45 2.519189919702452 2116 46-47 1.5290120596642005 2116 48-49 1.1894423193473997 2116 50-51 1.8769215869954188 2116 52-53 1.6961281175315293 2116 54-55 1.1073602302362602 2116 56-57 1.170512729664889 2116 58-59 0.03637368324211465 2116 60-61 -0.030461508695832862 2116 62-63 1.0997500755436356 2116 64-65 1.4205026373057663 2116 66-67 0.5745935282979246 2116 68-69 1.8495442086364484 2116 70-71 -1.2980420964786248 2116 72-73 0.7423118390872574 2116 74-75 -0.12432514914543447 2116 76 -0.9649737911336267 2117 1 0.34870903803158626 2117 2 0.44283247143763305 2117 3 0.9996377708494038 2117 4 -0.3004844625518288 2117 5 -1.6069833331019048 2117 6 -0.8380829081226224 2117 7 -0.899639355306487 2117 8 -1.8999628503193122 2117 9 1.317080108547028 2117 10-11 0.22666418502924301 2117 12-13 1.4102165487053036 2117 14-15 -0.48499330914643934 2117 16-17 0.586523412621851 2117 18-19 0.018883367231872228 2117 20-21 0.18834173549494793 2117 22-23 1.1394640337072097 2117 24-25 0.6561902801118791 2117 26-27 1.7783628693178954 2117 28-29 0.5582083158979181 2117 30-31 0.3760125455132268 2117 32-33 1.895463585389848 2117 34-35 0.3893950290768089 2117 36-37 -1.0693954764333036 2117 38-39 0.5921737551648576 2117 40-41 -1.1763698412127894 2117 42-43 -1.2427678308114274 2117 44-45 0.7988016382334244 2117 46-47 0.9500942015201055 2117 48-49 -0.5208534474033719 2117 50-51 1.3592947296923619 2117 52-53 1.3992750196902008 2117 54-55 -0.22835605796110414 2117 56-57 -0.0143414895669558 2117 58-59 -0.7190230086558387 2117 60-61 1.5275704509341068 2117 62-63 0.7773152563678565 2117 64-65 -0.24139811963284075 2117 66-67 -0.9665474865605006 2117 68-69 0.30770232470036163 2117 70-71 1.7109640711692577 2117 72-73 2.6394593019411907 2117 74-75 1.065540283991652 2117 76 0.4201537670385065 2118 1 0.8745711069971023 2118 2 0.6152462645410814 2118 3 0.904810184642507 2118 4 0.8115845029654096 2118 5 0.763706322070508 2118 6 1.3774343332566907 2118 7 1.9882916791762746 2118 8 -0.4603076779055222 2118 9 -0.8122302362805591 2118 10-11 0.22235384020165583 2118 12-13 0.8369406866363356 2118 14-15 -0.8168898608705746 2118 16-17 -0.5427869322057362 2118 18-19 -1.162151115526747 2118 20-21 -1.3030375748498813 2118 22-23 -0.3389842421548579 2118 24-25 0.18636269390498228 2118 26-27 -0.03198195826830741 2118 28-29 0.02372555727722414 2118 30-31 0.12170220068564319 2118 32-33 -0.3631571042653299 2118 34-35 0.1824984773526701 2118 36-37 -0.578016166088478 2118 38-39 -0.13627452069721357 2118 40-41 -1.0384388067300279 2118 42-43 0.08481837608512066 2118 44-45 0.186732672716186 2118 46-47 -0.44214717779024326 2118 48-49 -1.2105086198171655 2118 50-51 0.260156798657885 2118 52-53 -0.648138773413244 2118 54-55 0.14664394203889586 2118 56-57 0.6149688552606278 2118 58-59 0.0999425085855421 2118 60-61 -2.00691230768658 2118 62-63 0.15231525636785648 2118 64-65 -1.1250188092880151 2118 66-67 -1.302754383112223 2118 68-69 0.7775299109072584 2118 70-71 -1.8235186874514326 2118 72-73 -1.2355406980588022 2118 74-75 -0.2577355780773125 2118 76 -1.752260026064942 2119 1 0.1649159345833091 2119 2 1.702487643851427 2119 3 0.9099825984356116 2119 4 1.1605500202067915 2119 5 1.6081890806911971 2119 6 1.3888136436015195 2119 7 -1.0096393553064864 2119 8 1.106244046232412 2119 9 -0.3418854086943526 2119 10-11 1.1873538402016592 2119 12-13 1.1921131004294416 2119 14-15 -0.23068296431885216 2119 16-17 0.348419964345986 2119 18-19 0.4337109534387693 2119 20-21 -0.2733824024360878 2119 22-23 1.2468778268106604 2119 24-25 0.36412131459463737 2119 26-27 1.6456042486282385 2119 28-29 1.9104496952082535 2119 30-31 0.7141159937890933 2119 32-33 1.7689118612519081 2119 34-35 1.070084684249224 2119 36-37 0.1600872821873871 2119 38-39 1.2225185827510643 2119 40-41 0.49225084844238864 2119 42-43 0.18326665194718927 2119 44-45 0.4927671554748052 2119 46-47 0.9475079946235532 2119 48-49 0.8486293112173193 2119 50-51 2.4968809365889193 2119 52-53 1.0175508817591705 2119 54-55 0.624230148935446 2119 56-57 0.967382648364076 2119 58-59 2.440804577551056 2119 60-61 1.864294588865139 2119 62-63 0.5993842218850993 2119 64-65 1.2734294665740578 2119 66-67 1.4770732030946725 2119 68-69 0.8864954281486384 2119 70-71 0.9519985539278792 2119 72-73 -1.1739889739208742 2119 74-75 0.9693333874399279 2119 76 1.8029123877281599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 23.0 13 155.0 14 520.0 15 964.0 16 1390.0 17 1651.0 18 1749.0 19 1721.0 20 1443.0 21 1151.0 22 864.0 23 752.0 24 645.0 25 565.0 26 618.0 27 712.0 28 805.0 29 928.0 30 1179.0 31 1435.0 32 1768.0 33 2321.0 34 3326.0 35 4759.0 36 8064.0 37 9040.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.59223905374559 20.61838555716954 9.242581448433285 35.54679394065159 2 18.346035015447992 28.100926879505668 28.42018537590113 25.13285272914521 3 19.71987641606591 25.029866117404737 27.318228630278064 27.932028836251288 4 11.715756951596292 26.37075180226571 31.423274974253346 30.490216271884655 5 11.326467559217301 34.27394438722966 34.61997940267765 19.779608650875385 6 22.47167868177137 34.698249227600414 17.077239958805354 25.75283213182286 7 22.212152420185376 31.027806385169924 18.90422245108136 27.855818743563336 8 19.505664263645723 37.653964984552005 17.831101956745623 25.009268795056645 9 20.11740473738414 22.807415036045313 18.568486096807415 38.50669412976313 10-11 20.02574665293512 27.384140061791967 25.67765190525232 26.9124613800206 12-13 20.676622039134912 24.72811534500515 25.337796086508753 29.257466529351184 14-15 20.13491246138002 26.31719876416066 22.621009268795056 30.92687950566426 16-17 21.04943357363543 28.578784757981463 23.783728115345003 26.588053553038105 18-19 19.360453141091657 27.62821833161689 24.265705458290423 28.74562306900103 20-21 18.787847579814624 27.678681771369725 24.693099897013386 28.840370751802265 22-23 21.697219361483008 27.612770339855818 23.829042224510815 26.86096807415036 24-25 19.905252317198766 29.255406797116372 23.891864057672503 26.947476828012356 26-27 18.577754891864057 29.9124613800206 25.511843460350153 25.99794026776519 28-29 20.572605561277037 27.736354273944386 26.79093717816684 24.900102986611742 30-31 20.28630278063852 27.63233779608651 26.966014418125646 25.11534500514933 32-33 18.54994850669413 29.877445932028834 25.906282183316172 25.666323377960865 34-35 19.442842430484035 28.36354273944387 26.438722966014417 25.754891864057672 36-37 19.737384140061792 27.55715756951596 26.584963954685893 26.120494335736353 38-39 19.592173017507726 26.536560247167866 27.948506694129765 25.922760041194643 40-41 18.646755921730175 26.46549948506694 27.38928939237899 27.49845520082389 42-43 17.654994850669414 25.947476828012356 28.76313079299691 27.63439752832132 44-45 17.508753861997942 26.591143151390316 29.168898043254377 26.731204943357362 46-47 17.70442842430484 26.631307929969104 28.523171987641604 27.14109165808445 48-49 16.96086508753862 27.8043254376931 28.053553038105044 27.181256436663233 50-51 16.79196704428424 27.877445932028838 27.204943357363543 28.12564366632338 52-53 16.17198764160659 27.454170957775485 27.83419155509784 28.53964984552008 54-55 16.248197734294543 27.926879505664264 26.73326467559217 29.091658084449023 56-57 17.020597322348095 27.729145211122553 26.584963954685893 28.66529351184346 58-59 16.714727085478888 27.105046343975282 26.571575695159627 29.6086508753862 60-61 16.683831101956745 26.228630278063854 26.63748712667353 30.450051493305867 62-63 17.16477857878476 26.697219361483004 26.010298661174048 30.127703398558186 64-65 17.826982492276002 27.002059732234812 24.653964984552008 30.51699279093718 66-67 17.370751802265705 28.091658084449023 23.830072090628217 30.707518022657055 68-69 17.742533470648816 27.977342945417092 23.768280123583935 30.511843460350153 70-71 18.23274974253347 27.48197734294542 23.905252317198762 30.38002059732235 72-73 18.380020597322346 26.922760041194643 24.24304840370752 30.45417095777549 74-75 19.64160659114315 26.380020597322346 24.020597322348095 29.957775489186407 76 19.752832131822863 24.35839340885685 25.429454170957776 30.459320288362512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.5 10 1.0 11 1.5 12 2.0 13 2.5 14 5.0 15 5.5 16 7.5 17 11.0 18 10.0 19 14.5 20 15.0 21 10.0 22 18.5 23 37.0 24 55.5 25 64.0 26 95.5 27 143.5 28 177.5 29 195.0 30 234.0 31 349.0 32 525.5 33 626.0 34 713.0 35 961.0 36 1186.0 37 1250.0 38 1434.5 39 1819.0 40 2198.5 41 2530.0 42 2682.0 43 2848.0 44 3176.0 45 3404.0 46 3470.0 47 3456.0 48 3426.5 49 3268.5 50 3126.0 51 2994.0 52 2656.5 53 2300.0 54 2149.0 55 1985.0 56 1623.5 57 1264.0 58 1102.0 59 978.5 60 756.5 61 569.0 62 480.0 63 402.5 64 262.0 65 168.5 66 117.5 67 97.0 68 77.5 69 58.5 70 48.0 71 37.0 72 32.5 73 27.5 74 21.5 75 16.0 76 14.0 77 9.5 78 7.5 79 8.0 80 6.0 81 4.5 82 4.5 83 4.0 84 4.0 85 4.0 86 3.5 87 3.0 88 2.0 89 2.0 90 3.5 91 3.5 92 3.0 93 2.0 94 1.5 95 2.5 96 3.0 97 3.0 98 3.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.741503604531411 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48550.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.86096807415036 #Duplication Level Percentage of deduplicated Percentage of total 1 84.04973737806839 64.60144181256436 2 9.315039125308179 14.319258496395468 3 3.443563082859899 7.940267765190526 4 1.436381176975024 4.416065911431514 5 0.7423089291456747 2.8527291452111228 6 0.4314503162182442 1.9897013388259528 7 0.2304641440668882 1.239958805355304 8 0.14471004394897632 0.8898043254376931 9 0.08843391574659663 0.611740473738414 >10 0.11791188766212884 1.1390319258496395 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008238928939237899 0.0 2 0.0 0.0 0.0 0.008238928939237899 0.0 3 0.0 0.0 0.0 0.008238928939237899 0.0 4 0.0 0.0 0.0 0.010298661174047374 0.0 5 0.0 0.0 0.0 0.010298661174047374 0.0 6 0.0 0.0 0.0 0.010298661174047374 0.0 7 0.0 0.0 0.0 0.010298661174047374 0.0 8 0.0 0.0 0.0 0.010298661174047374 0.0 9 0.0 0.0 0.0 0.012358393408856848 0.0 10 0.0 0.0 0.0 0.012358393408856848 0.0 11 0.0 0.0 0.0 0.012358393408856848 0.0 12 0.0 0.0 0.0 0.014418125643666324 0.0 13 0.0 0.0 0.0 0.014418125643666324 0.0 14 0.0 0.0 0.0 0.014418125643666324 0.0 15 0.0 0.0 0.0 0.016477857878475798 0.0 16 0.0 0.0 0.0 0.018537590113285273 0.0 17 0.0 0.0 0.0 0.02265705458290422 0.0 18 0.0 0.0 0.0 0.028836251287332648 0.0 19 0.0 0.0 0.0 0.032955715756951595 0.0 20 0.0 0.0 0.0 0.035015447991761074 0.0 21 0.0 0.0 0.0 0.037075180226570546 0.0 22 0.0 0.0 0.0 0.03913491246138002 0.0 23 0.0 0.0 0.0 0.04531410916580844 0.0 24 0.0 0.0 0.0 0.05149330587023687 0.0 25 0.0 0.0 0.0 0.055612770339855816 0.0 26 0.0 0.0 0.0 0.06385169927909372 0.0 27 0.0 0.0 0.0 0.09474768280123584 0.0 28 0.0 0.0 0.0 0.12564366632337795 0.0 29 0.0 0.0 0.0 0.17301750772399588 0.0 30 0.0 0.0 0.0 0.2368692070030896 0.0 31 0.0 0.0 0.0 0.2801235839340886 0.0 32 0.0 0.0 0.0 0.33161688980432547 0.0 33 0.0 0.0 0.0 0.4140061791967044 0.0 34 0.0 0.0 0.0 0.4819773429454171 0.0 35 0.0 0.0 0.0 0.6055612770339855 0.0 36 0.0 0.0 0.0 0.768280123583934 0.0 37 0.0 0.0 0.0 1.1040164778578785 0.0 38 0.0 0.0 0.0 1.470648815653965 0.0 39 0.0 0.0 0.0 1.8949536560247169 0.0 40 0.0 0.0 0.0 2.481977342945417 0.0 41 0.0 0.0 0.0 3.209062821833162 0.0 42 0.0 0.0 0.0 4.028836251287332 0.0 43 0.0 0.0 0.0 4.9289392378990735 0.0 44 0.0 0.0 0.0 5.6663233779608655 0.0 45 0.0 0.0 0.0 6.3563336766220395 0.0 46 0.0 0.0 0.0 7.122554067971164 0.0 47 0.0 0.0 0.0 7.855818743563336 0.0 48 0.0 0.0 0.0 8.403707518022657 0.0 49 0.0 0.0 0.0 8.98249227600412 0.0 50 0.0 0.0 0.0 9.546858908341916 0.0 51 0.0 0.0 0.0 10.255406797116375 0.0 52 0.0 0.0 0.0 11.038105046343976 0.0 53 0.0 0.0 0.0 11.798146240988672 0.0 54 0.0 0.0 0.0 12.628218331616889 0.0 55 0.0 0.0 0.0 13.237899073120495 0.0 56 0.0 0.0 0.0 13.911431513903192 0.0 57 0.0 0.0 0.0 14.595262615859939 0.0 58 0.0 0.0 0.0 15.188465499485067 0.0 59 0.0 0.0 0.0 15.76930998970134 0.0 60 0.0 0.0 0.0 16.238928939237898 0.0 61 0.0 0.0 0.0 16.733264675592174 0.0 62 0.0 0.0 0.0 17.312049433573634 0.0 63 0.0 0.0 0.0 17.84346035015448 0.0 64 0.0 0.0 0.0 18.449021627188465 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTGC 20 0.006915822 52.493046 40 ACAGCCA 20 0.006915822 52.493046 32 AAAATTA 20 0.006915822 52.493046 40 GGGATAT 20 0.006915822 52.493046 13 CGACGTT 125 2.9956245E-6 22.397034 70 CAAAACA 95 0.0067460975 18.59093 1 TACACAA 100 0.009568561 17.497683 5 ACGACGT 160 2.5280127E-5 17.497683 69 GACGACG 200 0.0027185944 12.248378 68 CGACGAC 245 9.431847E-4 11.427057 68 TGCCGAC 445 1.2798046E-7 11.009778 70 CTGCCGA 500 6.361188E-7 9.798702 69 GCTGCCG 540 1.8166666E-6 9.072873 68 CGCTGCC 585 5.3629865E-6 8.374959 67 ACGCTGC 610 9.408606E-6 8.031724 66 GACGCTG 630 1.4484327E-5 7.7767477 65 CTGACGC 730 1.29540385E-5 7.190829 63 TGACGCT 685 4.4005406E-5 7.152337 64 ATCTGAC 825 9.684289E-6 6.78698 61 TCTGACG 775 2.9968318E-5 6.773297 62 >>END_MODULE