FastQCFastQC Report
Wed 25 May 2016
SRR1780428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48550
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT27105.581874356333676No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT26585.474768280123584TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC8791.8105046343975282RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC3750.772399588053553TruSeq Adapter, Index 9 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT2540.5231719876416066RNA PCR Primer, Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG2490.5128733264675592No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT1530.31513903192584963No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1090.22451081359423278No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1040.21421215242018538No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1040.21421215242018538No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT920.18949536560247168No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT800.164778578784758No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT790.1627188465499485No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC720.14830072090628219No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG710.1462409886714727No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT680.1400617919670443No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC660.13594232749742535TruSeq Adapter, Index 9 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC650.13388259526261587TruSeq Adapter, Index 9 (95% over 21bp)
CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG530.10916580844490217No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG530.10916580844490217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCAAA207.852502E-570.011
CCTGCAC150.00221205770.018
GATTACT150.00221205770.061
TTTACAC1450.060.344832
CTATACA352.9165676E-760.01
CTTTACA1650.053.0303041
TTACACA1650.053.0303043
ACACCAT200.006912200252.554
ATTACTC200.006912200252.562
ACATTAC200.006912200252.536
TTACTCC200.006912200252.563
TTTCCAA200.006912200252.510
GACCTGA200.006912200252.528
CTTTATA451.6524245E-646.6666682
TTTATAC503.412275E-642.03
TCTTTAT503.412275E-642.01
CCGAGCA501.6865195E-435.011
AAAATAT400.00239675935.066
AAAAAGA450.004268328731.11111367
ATCGTAT500.007143613427.99999836
Produced by FastQC (version 0.11.5)