FastQCFastQC Report
Wed 25 May 2016
SRR1780427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583500
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGAC300.00567892529.40032821
TTTACAC2750.026.3559862
TTACACA3050.024.4816063
AGTCGTG450.001410996724.403692
CGGTGGT1750.023.85611343
GCTATAC703.1956406E-522.0142636
TATACCG803.6655929E-621.961446
CTATACA1601.8189894E-1220.6171151
TGCCATA550.004466317620.01124424
GGTACGG550.004490796519.9923571
GCTAGTC550.004526598419.9649457
TCGGTGG3450.019.74869742
CTTTACA3700.019.6141221
CGAAACT909.956555E-619.5665524
CGTGCTT909.978734E-619.56150810
GTATCTA708.099827E-418.86288311
AATCTTG708.2080055E-418.8257032
TTAATAC708.2127366E-418.8240913
CATAACG600.00747925218.3027692
AGATTGG600.007482949618.30126
Produced by FastQC (version 0.11.5)