FastQCFastQC Report
Wed 25 May 2016
SRR1780427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583500
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT360246.173778920308483No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT299735.136760925449871No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC88471.5161953727506428No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC45520.7801199657240788No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG44490.7624678663239075No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT28250.4841473864610112No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC19010.3257926306769494No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC14820.25398457583547557No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11710.20068551842330765No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG8490.1455012853470437No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC7750.1328191945158526No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC7090.12150814053127677No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC6980.11962296486718081No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG5900.10111396743787489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT30350.042.91427244
GCCGTCT32600.039.95239643
TTTACAC10400.036.594372
CTTTACA10800.035.4609531
CTATACA5550.034.899131
TTACACA12150.031.8667623
CTTATAA1500.030.8143441
TAAGGAC502.3611628E-630.795865
CTTTATA2500.029.9185452
CGTGCGA300.005736760329.33944910
AAGTCGT300.00574638129.32939317
ATCACGC454.0697087E-529.32939129
TTATACA56750.028.5307712
TCTTTAT2800.028.298891
CTTATAC57650.028.2525271
CGTATTT407.0191844E-427.50573211
GGAGATC407.0227025E-427.50337640
TGCCGTC48350.026.94022442
TAACCGG508.336932E-526.39871422
TATACAC61850.026.3204653
Produced by FastQC (version 0.11.5)