##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780426_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15670 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.699425654116144 23.0 12.0 33.0 12.0 34.0 2 23.867900446713467 23.0 12.0 34.0 11.0 34.0 3 23.08136566687939 23.0 11.0 34.0 11.0 34.0 4 24.176196553924697 23.0 11.0 34.0 11.0 34.0 5 24.307913209955327 27.0 11.0 34.0 11.0 34.0 6 24.84396936821953 26.0 11.0 37.0 11.0 38.0 7 25.743139757498405 27.0 11.0 37.0 11.0 38.0 8 25.579578813018507 27.0 11.0 36.0 11.0 38.0 9 25.6401403956605 27.0 12.0 36.0 12.0 38.0 10-11 25.375239310784938 27.0 11.0 37.0 11.0 38.0 12-13 25.470197830248882 27.0 11.0 37.0 11.0 38.0 14-15 25.041480536056156 27.0 11.0 37.0 11.0 38.0 16-17 24.685098915124442 26.0 11.0 37.0 11.0 38.0 18-19 24.827536694320358 26.0 11.0 37.0 11.0 38.0 20-21 25.010944479897894 26.5 11.0 37.0 11.0 38.0 22-23 24.492501595405233 26.0 11.0 37.0 11.0 38.0 24-25 24.850478621569877 26.0 11.0 37.0 11.0 38.0 26-27 24.63854499042757 26.0 11.0 37.0 11.0 38.0 28-29 24.486056158264198 26.0 11.0 37.0 11.0 38.0 30-31 24.362986598596045 26.0 11.0 37.0 11.0 38.0 32-33 24.470165922144226 26.0 11.0 37.0 11.0 38.0 34-35 24.549680918953413 26.0 11.0 37.0 11.0 38.0 36-37 24.46850670070198 26.0 11.0 36.5 11.0 38.0 38-39 24.34055520102106 26.0 11.0 37.0 11.0 38.0 40-41 24.38024888321634 26.0 11.0 36.5 11.0 38.0 42-43 24.624441608168475 26.0 11.0 37.0 11.0 38.0 44-45 24.09977664326739 26.0 11.0 36.0 11.0 38.0 46-47 24.477377153797065 26.0 11.0 36.5 11.0 38.0 48-49 24.56097638800255 26.0 11.0 36.0 11.0 38.0 50-51 24.590587109125718 26.0 11.0 36.0 11.0 38.0 52-53 24.54103382259094 26.0 11.0 36.0 11.0 38.0 54-55 24.43315252074027 26.0 11.0 36.0 11.0 38.0 56-57 24.16553924696873 25.0 11.0 36.0 11.0 38.0 58-59 23.970325462667518 25.0 11.0 36.0 11.0 38.0 60-61 24.128015315890238 25.0 11.0 36.0 11.0 38.0 62-63 23.977409061901724 25.0 11.0 36.0 11.0 38.0 64-65 24.013337587747287 24.5 11.0 36.0 11.0 38.0 66-67 24.456253988513083 25.0 11.0 36.0 11.0 38.0 68-69 24.156126356094447 25.0 11.0 36.0 11.0 38.0 70-71 24.37817485641353 25.0 11.0 36.0 11.0 38.0 72-73 24.162061263560943 24.5 11.0 36.0 11.0 38.0 74-75 23.51828334396937 24.0 11.0 35.0 11.0 38.0 76 22.59974473516273 23.0 11.0 34.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07616988988660367 1101 2 2.9250331526064137 1101 3 0.5715756651757253 1101 4 1.5560557438366125 1101 5 -2.281725446961879 1101 6 -0.5546307383114488 1101 7 0.23849821401129745 1101 8 1.1871080547378305 1101 9 3.0375515776329003 1101 10-11 1.1146159561556281 1101 12-13 0.6285476017741942 1101 14-15 -0.27920129675672456 1101 16-17 0.45172652644286515 1101 18-19 -1.001819697190733 1101 20-21 -0.5901595089892382 1101 22-23 0.4810313420191399 1101 24-25 -1.0235816127759456 1101 26-27 0.6122620719789964 1101 28-29 0.6560368998071766 1101 30-31 0.7076119370088492 1101 32-33 -1.197117439270567 1101 34-35 0.13108762649736505 1101 36-37 0.18367118055057574 1101 38-39 1.2778198623696326 1101 40-41 1.3985576666993396 1101 42-43 0.36234666140668637 1101 44-45 -0.03652834037000474 1101 46-47 -0.14516729466102518 1101 48-49 0.3456607008674304 1101 50-51 -1.7616217689421063 1101 52-53 0.8321887509070152 1101 54-55 0.6432106168530289 1101 56-57 -0.013606838272416155 1101 58-59 0.5603461194262529 1101 60-61 -0.006468839541646787 1101 62-63 0.5007763159180278 1101 64-65 -0.6418430513238391 1101 66-67 -0.3242110811662222 1101 68-69 -0.8738912857993633 1101 70-71 1.2109537746940795 1101 72-73 -0.6532967802130898 1101 74-75 1.6772738508782936 1101 76 0.21792866674655187 1102 1 0.193952061332908 1102 2 -0.9292351400765106 1102 3 -1.66318043238525 1102 4 -1.0226027927487564 1102 5 -0.6195303250106612 1102 6 -1.3143868358724227 1102 7 -0.5182091030618707 1102 8 0.7066202498597818 1102 9 -1.2984240321231972 1102 10-11 1.0438842488385554 1102 12-13 -3.697367032372142 1102 14-15 -1.0087744674884327 1102 16-17 -0.6049807906303073 1102 18-19 0.29726566866292714 1102 20-21 -1.0078424358185067 1102 22-23 -0.2186637799320792 1102 24-25 -0.6891303932637527 1102 26-27 -3.201457440216128 1102 28-29 -0.9226216367781888 1102 30-31 -1.971656355674078 1102 32-33 -3.640105244148618 1102 34-35 -0.9859855442343388 1102 36-37 -0.8769995511567359 1102 38-39 -3.135289893727929 1102 40-41 -0.7011374552518781 1102 42-43 -0.5983240703006345 1102 44-45 -5.1078698037846415 1102 46-47 -1.2863258312463905 1102 48-49 -1.0451929576691548 1102 50-51 -3.3543046957713756 1102 52-53 -2.2309210051905453 1102 54-55 -1.6281308465616071 1102 56-57 -1.9556800090041229 1102 58-59 -1.1094709537444771 1102 60-61 0.1368238433851836 1102 62-63 -1.7010529523746563 1102 64-65 -0.3601357342506688 1102 66-67 -4.037320837263785 1102 68-69 -3.578464456531073 1102 70-71 -2.063131591159582 1102 72-73 -1.7725040972862622 1102 74-75 -5.80565297839 1102 76 -4.05219328447296 1103 1 0.147766280326163 1103 2 -1.142520038882946 1103 3 -1.5427860369519344 1103 4 -1.424263405099559 1103 5 0.31508306367641836 1103 6 -1.725907334056128 1103 7 -0.8928847647121039 1103 8 0.7849803951633625 1103 9 -1.843831401090501 1103 10-11 -0.7853840438443704 1103 12-13 -0.946718355672612 1103 14-15 -0.3725523605865142 1103 16-17 -2.660507516110325 1103 18-19 -1.1222984205949906 1103 20-21 0.9018617676065048 1103 22-23 -0.11072397712979765 1103 24-25 -1.1089539532014783 1103 26-27 -0.8672592046167473 1103 28-29 -1.1753460789162276 1103 30-31 -0.8551540204379613 1103 32-33 -2.5298302052280164 1103 34-35 -1.1795506713749724 1103 36-37 1.132341393316537 1103 38-39 0.6488305006675077 1103 40-41 -1.688942333300659 1103 42-43 -0.3868554662528929 1103 44-45 -3.014719829731707 1103 46-47 -1.368837507426985 1103 48-49 -1.328275469345332 1103 50-51 -0.9658770880910446 1103 52-53 -2.234034653348303 1103 54-55 -1.2583851278278253 1103 56-57 -2.021585561676673 1103 58-59 -2.529813455041829 1103 60-61 -1.4080645842224975 1103 62-63 0.27896780527973064 1103 64-65 -1.9748217747280954 1103 66-67 -1.774477038613032 1103 68-69 -2.527348732607873 1103 70-71 -3.5797377146676226 1103 72-73 -1.689732950425853 1103 74-75 -2.122726149121707 1103 76 -0.006007503466214814 1104 1 -0.1384247918473882 1104 2 0.15493511339072796 1104 3 0.985791351450235 1104 4 -0.2865913149869179 1104 5 0.5349412197047876 1104 6 1.3934084773748268 1104 7 3.1546746845995344 1104 8 -1.238382141340601 1104 9 -0.07078175570043044 1104 10-11 -1.5500899261973125 1104 12-13 -0.8614033786179576 1104 14-15 0.05339674245895765 1104 16-17 0.3718245656585495 1104 18-19 1.4861704988876987 1104 20-21 0.7260169615989973 1104 22-23 0.3523548714309044 1104 24-25 0.15950662251816894 1104 26-27 0.5144679543319377 1104 28-29 -0.4513160413692958 1104 30-31 2.6909452703421834 1104 32-33 0.0031276587686441815 1104 34-35 0.6840288029679549 1104 36-37 0.8236221609427332 1104 38-39 0.46777084276178726 1104 40-41 0.5267439412091477 1104 42-43 0.8571996025831545 1104 44-45 -1.7556459874288315 1104 46-47 1.0281170190644637 1104 48-49 2.8892881518478255 1104 50-51 1.29131940752848 1104 52-53 1.6956691430638813 1104 54-55 1.1750733619510676 1104 56-57 -0.1484107598410418 1104 58-59 0.7743167076615478 1104 60-61 0.8293154741838435 1104 62-63 1.6081292570945003 1104 64-65 1.5552157722055746 1104 66-67 1.9186810756965222 1104 68-69 -1.8104108936424979 1104 70-71 -0.31085995079611806 1104 72-73 0.22390910213985293 1104 74-75 0.7694307136233896 1104 76 1.7899874902759656 1105 1 0.21622141446122 1105 2 -0.8608364126109755 1105 3 -0.4664678130851456 1105 4 -1.2124225170329552 1105 5 0.17262237912507672 1105 6 -1.987239433963623 1105 7 0.8439329966199942 1105 8 -2.1868049887404304 1105 9 -0.7157092919323169 1105 10-11 -1.5027753481921984 1105 12-13 -0.9839523982258029 1105 14-15 -1.996049122843683 1105 16-17 -0.014577821383220169 1105 18-19 -0.3588849145820383 1105 20-21 0.2739709257933711 1105 22-23 -0.35864257102433683 1105 24-25 -1.5719511779933377 1105 26-27 -0.4893683628036136 1105 28-29 -1.7646152741058714 1105 30-31 -2.261953280382457 1105 32-33 -1.5444000479662208 1105 34-35 -1.7341297648069798 1105 36-37 -1.5429592542320307 1105 38-39 -1.5509844854564534 1105 40-41 -0.2650292898223974 1105 42-43 0.09441187879798818 1105 44-45 0.9841238335430376 1105 46-47 -0.20440642509580798 1105 48-49 -0.19020886434995887 1105 50-51 -1.8768391602464547 1105 52-53 -1.233572118658202 1105 54-55 0.19212366033128703 1105 56-57 -1.2907807513158929 1105 58-59 -1.2391104023128747 1105 60-61 -1.5010340569329514 1105 62-63 -1.1981367275602324 1105 64-65 -0.8961908774107954 1105 66-67 -0.7464936898618753 1105 68-69 -0.5222608510167532 1105 70-71 -1.1026331818276631 1105 72-73 -1.9755793889087379 1105 74-75 0.42075211174785565 1105 76 -1.5744626376012718 1106 1 -1.382122270838984 1106 2 -0.5523477997745374 1106 3 1.6608613794614406 1106 4 1.8548652676461366 1106 5 -1.2507730660095007 1106 6 0.8429883093076 1106 7 1.5706410711541565 1106 8 1.8680604356902109 1106 9 -1.4517341366528136 1106 10-11 0.5479492894889617 1106 12-13 -0.6982381125115182 1106 14-15 -0.20360605866148873 1106 16-17 0.8410122407285812 1106 18-19 0.9987755409045036 1106 20-21 -0.01277855660828564 1106 22-23 -0.3731353246475244 1106 24-25 0.2960612443669106 1106 26-27 0.4402382624551855 1106 28-29 -0.5451535763832993 1106 30-31 0.10761193700884775 1106 32-33 -0.14533172498485314 1106 34-35 -0.7760552306454862 1106 36-37 0.6247426091220092 1106 38-39 -0.29063251858274697 1106 40-41 0.6324862381279104 1106 42-43 -0.7632485766885573 1106 44-45 1.1337097548680894 1106 46-47 0.8054279434342142 1106 48-49 0.13018451039124201 1106 50-51 0.033616326295987875 1106 52-53 0.6018316080498742 1106 54-55 -0.005598906956496563 1106 56-57 1.4923455426799634 1106 58-59 -0.6640586424785049 1106 60-61 1.5709121128393058 1106 62-63 -0.8099379697962554 1106 64-65 1.5152998058190192 1106 66-67 0.8549555855004414 1106 68-69 0.35765633324825785 1106 70-71 0.1520252032655094 1106 72-73 -1.6479396373559467 1106 74-75 0.734416708021147 1106 76 -0.09516657134868467 1107 1 -0.8159847047014175 1107 2 -2.5444112918380277 1107 3 -1.275646557046496 1107 4 -0.983759070978202 1107 5 -1.4095032247396588 1107 6 -2.5750011086818176 1107 7 -3.0087240082109226 1107 8 -0.6425215748917985 1107 9 -0.6818928668115412 1107 10-11 -3.146495154955481 1107 12-13 -1.6598783241517268 1107 14-15 -1.0620716671270998 1107 16-17 -1.0769771772608365 1107 18-19 -0.424504882375917 1107 20-21 0.858914565084838 1107 22-23 -0.5768390283512304 1107 24-25 -1.4407112424055768 1107 26-27 -0.4539416317247067 1107 28-29 -0.7541482853780117 1107 30-31 -2.207202877805969 1107 32-33 -3.5077655874187137 1107 34-35 -2.168912373502632 1107 36-37 -1.8408658564864595 1107 38-39 -1.1596801376303674 1107 40-41 -1.3278312221895483 1107 42-43 -1.8307088941488736 1107 44-45 -1.3504172292588947 1107 46-47 -2.349333961327691 1107 48-49 -2.1700800398733087 1107 50-51 -0.1727328800532213 1107 52-53 0.6798739360921999 1107 54-55 -0.7040116053691925 1107 56-57 -1.1849031345687138 1107 58-59 -1.7262279546478219 1107 60-61 -1.2425799506527575 1107 62-63 0.6239244640661745 1107 64-65 -1.68443564391643 1107 66-67 -2.7191184885736313 1107 68-69 -0.23361350802158398 1107 70-71 0.2790093302496359 1107 72-73 -1.0963523357686462 1107 74-75 -2.3264298528254095 1107 76 -2.1904046665867796 1108 1 -0.6482287134160174 1108 2 -1.3744766513151525 1108 3 0.4955952730188642 1108 4 0.16438907716994322 1108 5 2.3584706314694905 1108 6 -0.8811013265467409 1108 7 -0.9825802173612495 1108 8 1.9184806037574376 1108 9 0.4390221658681952 1108 10-11 0.27343948556739406 1108 12-13 0.6287926998134132 1108 14-15 -0.37797580656064866 1108 16-17 1.0482951538938465 1108 18-19 -0.07265303052406935 1108 20-21 -0.23476735212649302 1108 22-23 0.8817666361367849 1108 24-25 0.30656544604758196 1108 26-27 0.798781679822131 1108 28-29 -1.1866101590163538 1108 30-31 1.220357035048064 1108 32-33 -0.16353900789802012 1108 34-35 0.6252052735561939 1108 36-37 1.5589162785897948 1108 38-39 -0.7479154317480123 1108 40-41 -1.9144325293790914 1108 42-43 -0.5643690248678261 1108 44-45 1.1855304831594076 1108 46-47 -0.7660006279943623 1108 48-49 -0.09110400501491966 1108 50-51 -0.6302492199225007 1108 52-53 -1.2455073275243578 1108 54-55 0.21428904822557726 1108 56-57 -1.5699793872920225 1108 58-59 -1.833526429593352 1108 60-61 -0.7981355062083111 1108 62-63 -0.8036354487878548 1108 64-65 -0.6506665807356029 1108 66-67 -0.8068091203819101 1108 68-69 -1.6143324622699495 1108 70-71 -1.4579187743255275 1108 72-73 -0.5604046233503439 1108 74-75 0.14197973323123492 1108 76 0.6527325883151818 1109 1 -1.6011698898866058 1109 2 -0.7799668473935846 1109 3 -0.1534243348242761 1109 4 -0.8089442561633895 1109 5 -0.031725446961878845 1109 6 0.23536926168855388 1109 7 0.6934982140112993 1109 8 -0.4928919452621692 1109 9 -0.1174484223670973 1109 10-11 0.1646159561556324 1109 12-13 -0.6539523982258046 1109 14-15 -1.2317012967567251 1109 16-17 -0.9632734735571375 1109 18-19 -1.1393196971907322 1109 20-21 0.814840491010763 1109 22-23 -0.5064686579808573 1109 24-25 -1.1310816127759473 1109 26-27 -1.9502379280210036 1109 28-29 0.47103689980717434 1109 30-31 -0.9323880629911514 1109 32-33 -0.8696174392705665 1109 34-35 0.011087626497367609 1109 36-37 -0.40382881944942284 1109 38-39 -0.4396801376303685 1109 40-41 -0.7789423333006589 1109 42-43 -0.8551533385933148 1109 44-45 -1.0715283403700049 1109 46-47 0.4873327053389751 1109 48-49 -0.24933929913256847 1109 50-51 -0.7716217689421079 1109 52-53 -0.7553112490929834 1109 54-55 0.07821061685302766 1109 56-57 -0.7686068382724152 1109 58-59 1.1678461194262546 1109 60-61 -0.5714688395416445 1109 62-63 1.5657763159180291 1109 64-65 -1.5918430513238384 1109 66-67 -0.09171108116622406 1109 68-69 0.9186087142006372 1109 70-71 -0.7965462253059208 1109 72-73 -2.300796780213087 1109 74-75 -0.8027261491217068 1109 76 0.2629286667465536 1110 1 -0.858312747029462 1110 2 -1.433300180726917 1110 3 1.208480427080488 1110 4 -0.8356109228300568 1110 5 -1.1555349707714058 1110 6 -0.9427259764066847 1110 7 -1.2150732145601282 1110 8 1.3680604356902109 1110 9 -0.7612579461766238 1110 10-11 -1.2734792819396112 1110 12-13 -0.9482381125115182 1110 14-15 1.6773463222908944 1110 16-17 -0.11136871165237139 1110 18-19 -0.26312922100025915 1110 20-21 -0.06039760422733309 1110 22-23 -0.3969448484570499 1110 24-25 -0.6563197080140419 1110 26-27 -1.5835712613543365 1110 28-29 -0.902296433526157 1110 30-31 -0.23762615822924715 1110 32-33 -1.085807915461043 1110 34-35 -0.6093885639788219 1110 36-37 -1.2919240575446587 1110 38-39 -0.7311087090589368 1110 40-41 -1.1651328094911335 1110 42-43 -1.8703914338314114 1110 44-45 0.5979954691538047 1110 46-47 -0.980286342280074 1110 48-49 -0.8936250134182835 1110 50-51 -0.03781224513258152 1110 52-53 -1.5648350586167936 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-3.874376102795967 2116 60-61 -2.0342466173194254 2116 62-63 -0.91311257297086 2116 64-65 0.8526013931206045 2116 66-67 -1.561711081166223 2116 68-69 -0.08083573024380541 2116 70-71 -1.9848795586392534 2116 72-73 -2.3880190024353105 2116 74-75 -2.789392815788375 2116 76 -1.4126268888090046 2117 1 0.05597296725625256 2117 2 1.7857474383207013 2117 3 0.08943280803286768 2117 4 1.0596271724080388 2117 5 0.23970312446668984 2117 6 -0.9046307383114467 2117 7 -0.47221607170298796 2117 8 -1.2557490881193125 2117 9 1.976837291918617 2117 10-11 0.4431873847270573 2117 12-13 1.4660476017742 2117 14-15 1.6368701318147032 2117 16-17 1.5267265264428644 2117 18-19 -0.6678911257621643 2117 20-21 -1.6294452232749528 2117 22-23 0.13638848487628152 2117 24-25 0.603204101509764 2117 26-27 0.9497620719789985 2117 28-29 1.6738940426643154 2117 30-31 1.9433262227231332 2117 32-33 0.28323970358657746 2117 34-35 0.031087626497367182 2117 36-37 0.6390283234077216 2117 38-39 0.8403198623696326 2117 40-41 1.8396290952707695 2117 42-43 1.517703804263828 2117 44-45 0.2670430882014223 2117 46-47 0.010189848196116458 2117 48-49 -0.16505358484685573 2117 50-51 1.1955210882007492 2117 52-53 0.773260179478445 2117 54-55 2.1467820454244553 2117 56-57 2.2042503045847255 2117 58-59 1.0835604051405419 2117 60-61 0.8113883033154963 2117 62-63 -0.23136654122482625 2117 64-65 2.551014091533304 2117 66-67 0.23828891883377779 2117 68-69 2.652894428486352 2117 70-71 1.0163109175512233 2117 72-73 1.3592032197869095 2117 74-75 2.1629881365925776 2117 76 -1.199928476110589 2118 1 0.7488301101133956 2118 2 0.09447759705085801 2118 3 -0.4700910014909425 2118 4 0.8310557438366111 2118 5 -1.2706143358507695 2118 6 -0.39907518275589027 2118 7 -1.453168452655369 2118 8 -2.0406697230399473 2118 9 0.151440466521791 2118 10-11 0.5062826228222939 2118 12-13 -0.8172857315591386 2118 14-15 0.36385425879883115 2118 16-17 0.11283763755397658 2118 18-19 -0.5032085860796229 2118 20-21 -1.048492842322574 2118 22-23 -2.1648019913141923 2118 24-25 0.2444739427796101 2118 26-27 1.5692065164234386 2118 28-29 1.375481344251618 2118 30-31 -1.8090547296578166 2118 32-33 0.5570492273960959 2118 34-35 2.4283098487195893 2118 36-37 1.0017267361061357 2118 38-39 -0.006902359852588802 2118 40-41 -0.0778312221895483 2118 42-43 1.8359577725177942 2118 44-45 1.4829161040744374 2118 46-47 0.3173327053389734 2118 48-49 1.3067718119785425 2118 50-51 1.49393378661345 2118 52-53 -0.6858668046485406 2118 54-55 1.4209883946308075 2118 56-57 0.791948717283141 2118 58-59 0.19506834164847575 2118 60-61 -0.7981355062083111 2118 62-63 -0.91311257297086 2118 64-65 -0.619620829101617 2118 66-67 -1.2005999700551122 2118 68-69 -0.483613508021584 2118 70-71 1.154009330249636 2118 72-73 0.2369809975646895 2118 74-75 -0.2477261491217071 2118 76 0.7818175556354419 2119 1 0.6195197652858084 2119 2 0.658653842261586 2119 3 -0.15687261068634228 2119 4 0.9345040196986787 2119 5 1.9717228289001874 2119 6 0.881576158240275 2119 7 0.42039476573543766 2119 8 -1.8577195314690655 2119 9 -1.1167587671946855 2119 10-11 -0.8888323197064416 2119 12-13 -0.7511937775361481 2119 14-15 -1.6054944002050036 2119 16-17 -2.9594803701088566 2119 18-19 -2.04966452477694 2119 20-21 -1.9737801986444126 2119 22-23 -0.25819279591189215 2119 24-25 0.7234011458447434 2119 26-27 -1.2329965487106627 2119 28-29 -0.724135513985928 2119 30-31 -0.28031909747391026 2119 32-33 -0.4285829565119492 2119 34-35 0.5379841782215031 2119 36-37 0.4375504908954042 2119 38-39 -0.625197379009677 2119 40-41 -2.413080264335143 2119 42-43 -0.23204989031745527 2119 44-45 -0.08221799554241827 2119 46-47 -0.13956384638516184 2119 48-49 0.424108976729503 2119 50-51 -1.0271390103214166 2119 52-53 -0.582897455989535 2119 54-55 -2.5162721417676615 2119 56-57 -0.5351585624103485 2119 58-59 -2.3849125012634005 2119 60-61 0.17887598804455962 2119 62-63 -1.9983616151164547 2119 64-65 0.58057074177961 2119 66-67 -0.00998694323518734 2119 68-69 -1.2269085271786722 2119 70-71 -1.3024082942714372 2119 72-73 -0.23045195262688267 2119 74-75 0.015204885361054465 2119 76 0.6946528046775882 >>END_MODULE >>Per sequence quality scores fail #Quality Count 10 1.0 11 0.0 12 18.0 13 171.0 14 457.0 15 811.0 16 1083.0 17 1308.0 18 1351.0 19 1287.0 20 1137.0 21 786.0 22 616.0 23 417.0 24 297.0 25 266.0 26 228.0 27 218.0 28 209.0 29 245.0 30 235.0 31 290.0 32 324.0 33 426.0 34 571.0 35 811.0 36 1154.0 37 953.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.942138687536083 25.90288023606389 23.125280646609788 33.02970042979024 2 8.28334396936822 22.986598596043393 30.465858328015315 38.26419910657307 3 18.117421825143587 25.41799617102744 27.185705169112957 29.278876834716016 4 7.102744097000638 37.40906190172304 25.539246968730055 29.94894703254627 5 7.051691129546906 31.920867900446716 23.962986598596043 37.064454371410335 6 22.507977026164646 33.38225909380983 14.39055520102106 29.719208679004467 7 12.776005105296745 33.06317804722399 13.96936821952776 40.1914486279515 8 10.446713465220165 32.62284620293555 14.703254626675175 42.22718570516911 9 15.34141671984684 24.11614550095724 14.409700063816208 46.132737715379704 10-11 13.063178047223994 27.472878111040206 24.514996809189533 34.948947032546265 12-13 12.297383535417996 25.223356732610082 24.208679004467136 38.270580727504786 14-15 15.264837268666243 28.350350989151245 20.810465858328016 35.5743458838545 16-17 20.13401403956605 28.66305041480536 19.530950861518825 31.671984684109766 18-19 16.502871729419272 27.125079770261646 19.894703254626673 36.477345245692405 20-21 14.1193363114231 27.93873643905552 21.139119336311424 36.80280791320995 22-23 20.960433950223354 27.86534779834078 18.206764518187622 32.96745373324825 24-25 17.38034460753031 32.319719208679004 18.028079132099553 32.27185705169113 26-27 14.412890874282066 33.47160178685386 21.665603063178047 30.449904275686023 28-29 17.57179323548181 28.72048500319081 24.910657306955965 28.797064454371412 30-31 17.294192724952136 28.331206126356097 25.676451818761965 28.6981493299298 32-33 14.039566049776644 33.73962986598596 23.43969368219528 28.78111040204212 34-35 14.298021697511167 31.73580089342693 23.104658583280152 30.861518825781747 36-37 15.015954052329292 28.98851308232291 24.716017868538607 31.279514996809187 38-39 15.430759412890874 25.335035098915128 27.932354818123805 31.3018506700702 40-41 13.675813656668796 26.684747925973195 26.094447989789405 33.54499042756861 42-43 11.435864709636249 25.46585832801532 28.356732610082965 34.74154435226547 44-45 11.630504148053605 26.975111678366304 29.94894703254627 31.44543714103382 46-47 12.399489470325463 29.527760051052965 26.40395660497766 31.668793873643907 48-49 11.94639438417358 31.719846841097638 22.900446713465218 33.43331206126356 50-51 11.713465220165922 29.814932992980214 22.603701340140393 35.86790044671346 52-53 11.119974473516272 29.853222718570514 23.75877472878111 35.2680280791321 54-55 11.442246330567963 30.2297383535418 22.437779195915763 35.890236119974475 56-57 14.4256541161455 29.73516273133376 20.89661774090619 34.94256541161455 58-59 13.599234205488195 28.60880663688577 21.818761965539245 35.97319719208679 60-61 11.924058710912572 27.63241863433312 22.15698787492023 38.28653477983408 62-63 13.13018506700702 28.60880663688577 20.848755583918315 37.412252712188895 64-65 14.039566049776644 29.234205488194004 19.1576260370134 37.56860242501595 66-67 12.067645181876197 34.07147415443523 17.259093809827696 36.60178685386088 68-69 12.456924058710912 35.52967453733248 16.665603063178047 35.34779834077856 70-71 11.08487555839183 36.36247606892151 17.322910019144864 35.2297383535418 72-73 11.790044671346521 36.9527760051053 17.479259731971922 33.777919591576264 74-75 13.605615826419912 36.42629227823867 17.373962986598595 32.59412890874282 76 15.74345883854499 34.26292278238673 17.683471601786856 32.31014677728143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.0 14 3.0 15 3.0 16 3.5 17 4.0 18 5.5 19 7.5 20 6.5 21 5.0 22 9.0 23 16.5 24 19.5 25 19.0 26 33.5 27 54.0 28 60.0 29 60.0 30 91.0 31 136.5 32 172.0 33 193.0 34 215.5 35 271.5 36 345.0 37 385.0 38 426.0 39 507.5 40 617.0 41 746.5 42 807.0 43 831.5 44 886.5 45 941.0 46 965.0 47 980.0 48 1006.5 49 993.0 50 968.0 51 944.5 52 843.5 53 759.0 54 752.0 55 708.5 56 598.0 57 492.0 58 453.0 59 418.5 60 349.0 61 283.5 62 253.0 63 231.0 64 165.0 65 112.5 66 91.5 67 79.0 68 64.5 69 51.5 70 40.5 71 28.0 72 26.5 73 19.0 74 14.5 75 16.0 76 11.5 77 7.5 78 5.5 79 3.0 80 4.0 81 4.0 82 3.5 83 4.0 84 3.0 85 1.5 86 1.5 87 2.0 88 2.0 89 2.5 90 2.5 91 1.5 92 1.0 93 1.0 94 3.5 95 5.0 96 4.0 97 2.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5169112954690491 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 15670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.78430121250798 #Duplication Level Percentage of deduplicated Percentage of total 1 95.04627109417528 89.13848117421826 2 3.7289058247142077 6.994256541161454 3 0.9322264561785519 2.6228462029355457 4 0.21094175285792052 0.7913209955328654 5 0.04763200870985302 0.22335673261008296 6 0.020413718018508435 0.1148691767708998 7 0.0 0.0 8 0.006804572672836146 0.05105296745373325 9 0.0 0.0 >10 0.006804572672836146 0.06381620931716657 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.012763241863433313 0.0 6 0.0 0.0 0.0 0.012763241863433313 0.0 7 0.0 0.0 0.0 0.012763241863433313 0.0 8 0.0 0.0 0.0 0.012763241863433313 0.0 9 0.0 0.0 0.0 0.012763241863433313 0.0 10 0.0 0.0 0.0 0.012763241863433313 0.0 11 0.0 0.0 0.0 0.012763241863433313 0.0 12 0.0 0.0 0.0 0.012763241863433313 0.0 13 0.0 0.0 0.0 0.012763241863433313 0.0 14 0.0 0.0 0.0 0.012763241863433313 0.0 15 0.0 0.0 0.0 0.012763241863433313 0.0 16 0.0 0.0 0.0 0.012763241863433313 0.0 17 0.0 0.0 0.0 0.012763241863433313 0.0 18 0.0 0.0 0.0 0.03190810465858328 0.0 19 0.0 0.0 0.0 0.03828972559029994 0.0 20 0.0 0.0 0.0 0.05105296745373325 0.0 21 0.0 0.0 0.0 0.05105296745373325 0.0 22 0.0 0.0 0.0 0.05105296745373325 0.0 23 0.0 0.0 0.0 0.07019783024888322 0.0 24 0.0 0.0 0.0 0.07657945118059988 0.0 25 0.0 0.0 0.0 0.07657945118059988 0.0 26 0.0 0.0 0.0 0.07657945118059988 0.0 27 0.0 0.0 0.0 0.07657945118059988 0.0 28 0.0 0.0 0.0 0.09572431397574985 0.0 29 0.0 0.0 0.0 0.1021059349074665 0.0 30 0.0 0.0 0.0 0.13401403956604976 0.0 31 0.0 0.0 0.0 0.1467772814294831 0.0 32 0.0 0.0 0.0 0.17868538608806636 0.0 33 0.0 0.0 0.0 0.2297383535417996 0.0 34 0.0 0.0 0.0 0.2999361837906828 0.0 35 0.0 0.0 0.0 0.3318442884492661 0.0 36 0.0 0.0 0.0 0.4467134652201659 0.0 37 0.0 0.0 0.0 0.593490746649649 0.0 38 0.0 0.0 0.0 0.8104658583280153 0.0 39 0.0 0.0 0.0 1.1040204211869815 0.0 40 0.0 0.0 0.0 1.410338225909381 0.0 41 0.0 0.0 0.0 1.7421825143586471 0.0 42 0.0 0.0 0.0 2.0485003190810467 0.0 43 0.0 0.0 0.0 2.4569240587109125 0.0 44 0.0 0.0 0.0 2.833439693682195 0.0 45 0.0 0.0 0.0 3.114231014677728 0.0 46 0.0 0.0 0.0 3.4077855775366945 0.0 47 0.0 0.0 0.0 3.707721761327377 0.0 48 0.0 0.0 0.0 3.9374601148691766 0.0 49 0.0 0.0 0.0 4.14167198468411 0.0 50 0.0 0.0 0.0 4.2756860242501595 0.0 51 0.0 0.0 0.0 4.601148691767709 0.0 52 0.0 0.0 0.0 4.881940012763242 0.0 53 0.0 0.0 0.0 5.1435864709636245 0.0 54 0.0 0.0 0.0 5.488194001276324 0.0 55 0.0 0.0 0.0 5.705169112954691 0.0 56 0.0 0.0 0.0 5.979578813018507 0.0 57 0.0 0.0 0.0 6.190172303765157 0.0 58 0.0 0.0 0.0 6.509253350350989 0.0 59 0.0 0.0 0.0 6.713465220165922 0.0 60 0.0 0.0 0.0 6.860242501595406 0.0 61 0.0 0.0 0.0 7.0708359923420545 0.0 62 0.0 0.0 0.0 7.243139757498405 0.0 63 0.0 0.0 0.0 7.581365666879387 0.0 64 0.0 0.0 0.0 7.849393746011487 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCAGC 15 0.002184407 69.9968 12 CGACCTC 15 0.002184407 69.9968 5 AAAGAAT 15 0.002184407 69.9968 70 ACACTCC 25 2.3191841E-4 55.997444 6 CTATACA 20 0.006826465 52.4976 2 CCCACAT 40 0.0023480179 34.9984 18 >>END_MODULE