FastQCFastQC Report
Wed 25 May 2016
SRR1780426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15670
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT227314.50542437779196No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT191912.246330567964263TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC7354.690491384811742No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC3152.0102105934907466No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1961.2507977026164645No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1741.1104020421186982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC1300.8296107211231653No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1240.7913209955328654No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC990.6317804722399489No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT870.5552010210593491No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT860.5488194001276324No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG820.5232929164007658No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT790.5041480536056158No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT760.48500319081046583No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG650.4148053605615826No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC610.389278876834716No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT520.3318442884492661No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC500.3190810465858328No Hit
TTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC390.24888321633694957No Hit
CTTATCACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC390.24888321633694957No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG370.2361199744735163No Hit
CTTATTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG330.21059349074664963No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGACTCTCGTATGCCGTCTT310.19783024888321635No Hit
CTTATACACATCTCCGAGCCCACGCGACGGCGTCGAATCTCGTATGCCGT260.16592214422463306No Hit
ACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCT260.16592214422463306RNA PCR Primer, Index 8 (95% over 23bp)
TCTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT240.15315890236119975No Hit
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC240.15315890236119975No Hit
CTTATACACATCTCCGAGCCCACGAGCCGGCGTCGAATCTCGTATGCCGT240.15315890236119975No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGTCTT230.14677728142948307TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTC220.14039566049776644No Hit
CTTATACACATCTCCGAGCACACGAGACGGCGTCGAATCTCGTATGCCGT210.13401403956604976No Hit
CTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT210.13401403956604976No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCAGT210.13401403956604976No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCG200.12763241863433314No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGT200.12763241863433314No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAGCTCGTATGCCGT190.12125079770261646No Hit
CTTATATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC190.12125079770261646No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTAGTATGCCGT180.1148691767708998No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTCTGCCGTCTT170.10848755583918315No Hit
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTC170.10848755583918315No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC160.1021059349074665No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTG150.002184011370.0000112
TTATTAC150.002184011370.000012
ATTACAC150.002184011370.000014
TTTACAC650.070.000012
CTTTACA700.065.01
TTACACA800.056.8753
TCTTTAT252.3186608E-456.0000041
AAACATT200.006825232452.569
CACCGCT200.006825232452.511
CTTATTA200.006825232452.51
TATTACA200.006825232452.53
AAAAATG200.006825232452.568
CTTCTAC200.006825232452.51
TTTATAC351.993733E-550.03
CTTTATA305.702485E-446.6666682
CTATACA551.2050805E-744.5454521
CTTATAC5850.043.0769271
TTATACA6100.041.3114742
CACGAGC350.001218205340.021
AGCCGGC350.001218205340.025
Produced by FastQC (version 0.11.5)