FastQCFastQC Report
Wed 25 May 2016
SRR1780425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171997
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC401.8229719E-532.9683764
CTTTACA1450.031.850061
GATAGAG300.005685097529.3821218
ATGATTT300.005709452629.35644134
TTGCAAG300.005709452629.35644140
TGCATAA300.005733890529.3308113
TAACTAG300.00574205529.32227725
GATATCG454.068001E-529.3137541
TGCCCGA300.00575022829.31374743
TTTACAC1700.028.4433042
CATCCAT406.939656E-427.54573419
AGCCGTC406.975234E-427.52166435
AGATATC406.975234E-427.52166440
CAGATAA406.975234E-427.52166437
ATGCTGC406.9990364E-427.5056414
ATATCGG508.3384366E-526.38237442
TAAGAGA508.354962E-526.37474
TAATATA609.761992E-625.6420734
TTACACA2050.024.6592753
CCGGCAC450.001379909324.48509817
Produced by FastQC (version 0.11.5)