FastQCFastQC Report
Wed 25 May 2016
SRR1780425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171997
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3435719.97534840723966No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT2769616.102606440810014RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC86365.021017808450147No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC45442.641906544881597No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG37062.1546887445711262No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT26821.5593295231893578RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC21651.2587428850503208No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC16770.9750170061105716No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12000.6976865875567597No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9250.5378000779083356No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG8300.4825665563934255No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC6210.36105280906062315No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC5570.32384285772426263No Hit
CTTATTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5220.3034936655871905No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG4970.28895852834642466No Hit
CTTATCACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC4310.25058576603080285No Hit
ACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTTCT3420.19884067745367653RNA PCR Primer, Index 17 (95% over 24bp)
CTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT3170.18430554021291068RNA PCR Primer, Index 17 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2710.15756088768990156No Hit
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT2520.14651418338691954No Hit
TCTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT2400.13953731751135195No Hit
ATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTTCTGCTT2310.13430466810467626Illumina PCR Primer Index 9 (96% over 27bp)
TCTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGC1970.11453688145723472No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT1790.10407158264388332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACT207.8176515E-444.0229841
ACTCTAT254.420968E-544.01017811
TACTCTA254.420968E-544.01017810
ATTTACT254.4285698E-543.9973837
CACGGGG254.4285698E-543.99738330
GCTCTTA254.4285698E-543.9973837
TCAGACA254.4285698E-543.99738334
AGCGGAT207.8400923E-443.9973835
GGAACTA207.8400923E-443.9973828
AATCGGC207.8400923E-443.997386
CCGTCTT29700.042.5900644
CTTATAA1400.042.4507331
TTTACAC8550.041.167142
CTTTACA8650.040.9693681
CTTATAT501.3260433E-939.6206861
TCACATC501.3351382E-939.5976455
TCTTTAT1700.038.8438071
CTATACA4650.038.34261
GCCGTCT33150.038.1576143
TATCAGA301.2969514E-436.66448632
Produced by FastQC (version 0.11.5)