FastQCFastQC Report
Wed 25 May 2016
SRR1780424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAG200.006942974452.4930230
GACCCAT200.006942974452.4930247
GCCGTTG200.006942974452.4930228
ACGCTTC350.001252805239.99468215
GACGATA400.002413764134.99534643
GCTCTAA604.701307E-429.4367181
AGATACC604.9660413E-429.1627927
TAGGCCA604.9660413E-429.162795
CTATAGG500.00719371227.9962792
ATGTCTG500.00719371227.99627933
GTGACGA500.00719371227.99627941
CACAGCT657.9303526E-426.919517
GAACAGT700.001222114224.99667729
TGCCGCC800.002657600421.87209119
CCACAGC1003.913335E-420.9972116
CTTTACA1055.1060144E-420.1851771
TAGGGTT1257.771679E-519.5973954
CATGGGC900.005258193219.44185833
AAAGAAA950.00680758218.591611
GCATAGT950.00680758218.591611
Produced by FastQC (version 0.11.5)