##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139737 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.373666244444923 34.0 27.0 34.0 12.0 34.0 2 30.403085796889872 34.0 32.0 34.0 21.0 34.0 3 30.572317997380793 34.0 32.0 34.0 21.0 34.0 4 30.6770289901744 34.0 33.0 34.0 21.0 34.0 5 30.893313868195253 34.0 33.0 34.0 21.0 34.0 6 33.74960103623235 38.0 34.0 38.0 21.0 38.0 7 33.002304328846336 37.0 34.0 38.0 11.0 38.0 8 32.10224206902968 37.0 31.0 38.0 11.0 38.0 9 32.84035724253419 37.0 33.0 38.0 12.0 38.0 10-11 33.03265420039074 37.0 34.0 38.0 11.0 38.0 12-13 33.30611076522324 38.0 34.0 38.0 16.0 38.0 14-15 32.964007385302395 37.0 34.0 38.0 11.0 38.0 16-17 32.812655202272836 37.0 33.0 38.0 11.5 38.0 18-19 32.85782219455119 37.0 32.5 38.0 11.0 38.0 20-21 32.918296514165895 37.0 33.5 38.0 11.0 38.0 22-23 32.83831411866578 37.0 33.5 38.0 11.0 38.0 24-25 33.18792803623951 37.5 34.0 38.0 15.0 38.0 26-27 33.18094348669286 38.0 34.0 38.0 11.5 38.0 28-29 33.26584226081854 38.0 34.0 38.0 11.0 38.0 30-31 33.13509664584183 38.0 34.0 38.0 11.0 38.0 32-33 32.9383377344583 37.0 34.0 38.0 11.0 38.0 34-35 32.88296585728905 37.0 34.0 38.0 11.0 38.0 36-37 32.846915992185316 37.0 33.5 38.0 11.0 38.0 38-39 33.23240802364442 37.5 34.0 38.0 11.0 38.0 40-41 32.81855557225359 37.5 33.5 38.0 11.0 38.0 42-43 33.12344976634678 37.0 34.0 38.0 11.0 38.0 44-45 32.31592563172245 37.0 31.0 38.0 11.0 38.0 46-47 32.95056427431532 37.0 34.0 38.0 11.0 38.0 48-49 32.76765280491209 37.0 33.0 38.0 11.0 38.0 50-51 32.72720181483787 37.0 32.5 38.0 11.0 38.0 52-53 32.69179959495338 37.0 32.0 38.0 11.0 38.0 54-55 32.4600177476259 37.0 31.0 38.0 11.0 38.0 56-57 32.48925123625096 37.0 31.5 38.0 11.0 38.0 58-59 32.38014985293802 37.0 31.5 38.0 11.0 38.0 60-61 32.43425864302225 37.0 31.0 38.0 11.0 38.0 62-63 32.47718929131153 37.0 31.0 38.0 11.0 38.0 64-65 32.54034722371312 37.0 31.5 38.0 11.0 38.0 66-67 32.52129357292628 37.0 31.0 38.0 11.0 38.0 68-69 32.15762468065008 37.0 30.5 38.0 11.0 38.0 70-71 32.098059211232524 37.0 31.0 38.0 11.0 38.0 72-73 32.18029226332324 37.0 31.0 38.0 11.0 38.0 74-75 31.762768629639968 37.0 29.0 38.0 11.0 38.0 76 31.087235306325454 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.990761401303999 1101 2 -0.7199562295570558 1101 3 0.010421230266757675 1101 4 -1.848891026348383 1101 5 -0.7702471187748294 1101 6 -1.9175727373092109 1101 7 -1.0092743915251887 1101 8 0.20349758950534635 1101 9 -0.5134618688854147 1101 10-11 -0.9637514748728222 1101 12-13 -0.8942830278702587 1101 14-15 0.04054627095505481 1101 16-17 -1.4652174038130994 1101 18-19 -0.5316556457510728 1101 20-21 -1.0797157568256992 1101 22-23 -0.236655950871409 1101 24-25 -1.3181485344436652 1101 26-27 -1.4291470645504738 1101 28-29 -0.43993290648738537 1101 30-31 -0.9615869891575244 1101 32-33 -0.04582167811054916 1101 34-35 -0.6319668975988861 1101 36-37 -0.07426182270997828 1101 38-39 -0.8389136608979442 1101 40-41 0.16678396577419363 1101 42-43 -0.10666777173965869 1101 44-45 -0.4421579714139874 1101 46-47 0.2152927430409406 1101 48-49 -0.8797759398469509 1101 50-51 -0.42830789284997905 1101 52-53 -0.10187370828990794 1101 54-55 -1.2668820356127561 1101 56-57 -0.4464743817183532 1101 58-59 -0.12582048667547951 1101 60-61 0.17911565825653497 1101 62-63 -1.374497640647391 1101 64-65 -0.5620730996530057 1101 66-67 -1.2177405902674394 1101 68-69 0.16783512442508197 1101 70-71 0.2728444798599199 1101 72-73 -0.5417633301369378 1101 74-75 -1.1813928318175222 1101 76 -1.6693809119121532 1102 1 0.40207686508077956 1102 2 -0.27485844943020865 1102 3 0.13073042688409586 1102 4 -0.24199356334626998 1102 5 0.41778142245138383 1102 6 0.576547241549136 1102 7 0.9574935788765018 1102 8 1.4582544605412906 1102 9 1.0750687865057102 1102 10-11 -0.17397874760009557 1102 12-13 -3.427812361908316 1102 14-15 -0.68573544151851 1102 16-17 -2.3035168224177482 1102 18-19 -0.46641383814008464 1102 20-21 0.03983366177894965 1102 22-23 -0.7545933187361129 1102 24-25 0.0895549539284275 1102 26-27 -4.881875658630811 1102 28-29 -0.2966652003563013 1102 30-31 -0.012260879220953314 1102 32-33 -6.384451434981585 1102 34-35 -0.5732327538356756 1102 36-37 0.5290019193619102 1102 38-39 0.40979801986315323 1102 40-41 0.15274458945284408 1102 42-43 0.1824773128269399 1102 44-45 -4.928448933359018 1102 46-47 -0.2156004916313563 1102 48-49 0.19696163943423528 1102 50-51 -5.681247903420804 1102 52-53 -1.296839353110201 1102 54-55 0.35352626248449326 1102 56-57 -1.2420610624794506 1102 58-59 0.6608470608932322 1102 60-61 0.01817485487387671 1102 62-63 -4.014594099421174 1102 64-65 -0.10752764510755242 1102 66-67 -0.190355547984133 1102 68-69 -7.255460330120371 1102 70-71 -0.25440842711682166 1102 72-73 0.17381542250577553 1102 74-75 -5.242142039005682 1102 76 -1.1473803833709262 1103 1 -0.28062642409236105 1103 2 -0.28515139141284607 1103 3 0.4843022633390959 1103 4 0.8093712150913746 1103 5 0.22851120774147304 1103 6 -1.2409921600040832 1103 7 0.4185916830336538 1103 8 1.7771215231269437 1103 9 0.6293744055098216 1103 10-11 1.2011354598005468 1103 12-13 -0.24174709237884429 1103 14-15 0.41486700251986264 1103 16-17 0.08051270019531387 1103 18-19 -0.19619199026433876 1103 20-21 -0.20836043369843793 1103 22-23 -0.09719410676717644 1103 24-25 0.867350180059077 1103 26-27 0.051862635110616395 1103 28-29 0.8368361130288022 1103 30-31 0.4102065716698604 1103 32-33 -4.138998317119551 1103 34-35 -0.40130778809789547 1103 36-37 0.24603880835231706 1103 38-39 0.9539605940979641 1103 40-41 0.9447800508507385 1103 42-43 0.5475626828589171 1103 44-45 -3.8355055231260344 1103 46-47 0.09864117122839389 1103 48-49 0.2780845837010162 1103 50-51 0.17694219479756157 1103 52-53 -0.12979675374976551 1103 54-55 0.8756182565457138 1103 56-57 1.0731513632857386 1103 58-59 0.8663204505688853 1103 60-61 1.1630469425851615 1103 62-63 0.637765710994536 1103 64-65 0.6125500159248816 1103 66-67 0.11408569230822962 1103 68-69 -1.9398910062281836 1103 70-71 0.2718949648429785 1103 72-73 1.0460887208154972 1103 74-75 -0.7529132244785011 1103 76 1.2176121931480317 1104 1 -1.7535580927448997 1104 2 -0.12018998644988699 1104 3 0.2975825824604783 1104 4 0.12521590244567093 1104 5 -0.1632284182234045 1104 6 -0.5332524304283695 1104 7 0.7334132133657221 1104 8 1.2993978532310706 1104 9 -0.04079344404724594 1104 10-11 0.035604195230270363 1104 12-13 -0.20398094203952155 1104 14-15 0.03969215923125091 1104 16-17 -0.5771959461290876 1104 18-19 0.5864065695450194 1104 20-21 1.0155102081707028 1104 22-23 0.3278369764841358 1104 24-25 0.6575317580521371 1104 26-27 0.780305105192987 1104 28-29 -0.0910507439364352 1104 30-31 -0.6003876364843137 1104 32-33 -0.3089330422917982 1104 34-35 -0.07214910809996411 1104 36-37 -0.014548924124504481 1104 38-39 -0.01093475895596896 1104 40-41 0.5078142942325954 1104 42-43 0.8444530625926419 1104 44-45 -2.270016998026307 1104 46-47 0.3287996958100692 1104 48-49 0.6616260021333886 1104 50-51 1.0383367966729153 1104 52-53 0.15533379590573304 1104 54-55 0.11033565798590672 1104 56-57 0.3615003245870909 1104 58-59 0.8406443898529332 1104 60-61 0.31550381457397236 1104 62-63 0.6287839465019758 1104 64-65 0.08354545704802518 1104 66-67 0.3763765279296436 1104 68-69 -3.0261081745439924 1104 70-71 -0.0377524992817726 1104 72-73 0.8380316830493584 1104 74-75 -0.7660847522203014 1104 76 0.1478691480255101 1105 1 -0.8635653317054874 1105 2 -0.1362592308521755 1105 3 -0.5903445786188755 1105 4 -0.22167384069603813 1105 5 0.1909626903360433 1105 6 -0.3170321663311171 1105 7 0.7049690906528596 1105 8 0.35347788118843937 1105 9 0.337698106338415 1105 10-11 0.05123036532088321 1105 12-13 -1.5479938223969754 1105 14-15 -0.29675101492815514 1105 16-17 0.5479132623280165 1105 18-19 0.3117441796895548 1105 20-21 -0.5873984810781891 1105 22-23 0.01632141271888088 1105 24-25 0.35430979654343986 1105 26-27 -0.09511924766889734 1105 28-29 -0.8228971781243004 1105 30-31 -0.07183742555597661 1105 32-33 0.21064335370429887 1105 34-35 0.49985358778879885 1105 36-37 0.2161648623511212 1105 38-39 0.18415210857105535 1105 40-41 0.19614583862739465 1105 42-43 0.00206582812520395 1105 44-45 0.9475106473145445 1105 46-47 0.610863298815481 1105 48-49 -0.34291082919882854 1105 50-51 -0.18705697782097985 1105 52-53 -0.17869743147252137 1105 54-55 -0.5059931905201296 1105 56-57 0.1716523427593799 1105 58-59 -0.04362272779265908 1105 60-61 -0.017291797118197394 1105 62-63 0.19763831858454495 1105 64-65 0.2054813797658781 1105 66-67 0.14942887310324693 1105 68-69 0.6144150275727753 1105 70-71 0.8365797690090844 1105 72-73 0.3961836263358407 1105 74-75 -0.9601388197672946 1105 76 -0.4710307795848827 1106 1 -0.19791369145410087 1106 2 0.07972005724317555 1106 3 -0.734409021617541 1106 4 -0.5068984991307062 1106 5 -0.4730993380623403 1106 6 -0.31098895840049323 1106 7 0.24423652775587357 1106 8 1.3283123804607015 1106 9 0.9241307356369077 1106 10-11 0.3601751179249675 1106 12-13 0.2220121470314993 1106 14-15 0.0604320855527547 1106 16-17 -0.11354971715459072 1106 18-19 -0.22126541834657587 1106 20-21 -0.2326299808803256 1106 22-23 -0.33563005381543576 1106 24-25 0.4703548154242725 1106 26-27 0.10422277375398181 1106 28-29 -0.996610900431854 1106 30-31 -0.498544246131047 1106 32-33 0.6278762217799354 1106 34-35 0.9083603587304268 1106 36-37 0.6443774544915968 1106 38-39 0.37223270256272656 1106 40-41 -0.0015026089858380942 1106 42-43 0.7800165032072002 1106 44-45 0.8745092320904888 1106 46-47 0.44937684405234535 1106 48-49 0.223131842153947 1106 50-51 0.6135574038066025 1106 52-53 -0.20269587539677758 1106 54-55 0.8383891748491337 1106 56-57 0.43812025205089355 1106 58-59 -0.04257304739183354 1106 60-61 0.5824848523556199 1106 62-63 0.7732387827243841 1106 64-65 0.9434670665519747 1106 66-67 0.797450577676905 1106 68-69 0.9896841548959969 1106 70-71 0.3114243291158658 1106 72-73 0.3934940299094478 1106 74-75 -0.8112485298140442 1106 76 -0.28965405499016583 1107 1 -0.6650422689024467 1107 2 -0.07642697130733112 1107 3 0.2918687741976278 1107 4 0.2897594405077335 1107 5 0.16292141338314536 1107 6 -0.2401964106839145 1107 7 0.08805495951580866 1107 8 1.0325006632164175 1107 9 -0.7234746276860875 1107 10-11 -0.2157776594114722 1107 12-13 0.266492359243351 1107 14-15 -0.6454313721464899 1107 16-17 0.2780371052630528 1107 18-19 0.6301310213022191 1107 20-21 0.4133140233749586 1107 22-23 -0.3059811842994691 1107 24-25 -0.5794755946999999 1107 26-27 0.27096312509170417 1107 28-29 -0.5778620661463734 1107 30-31 -0.319511219279903 1107 32-33 0.6754166504865609 1107 34-35 0.16960475466579794 1107 36-37 -0.5314364732928212 1107 38-39 0.6285586467051374 1107 40-41 0.2878294624975055 1107 42-43 0.26861495168434857 1107 44-45 -0.09566229200785159 1107 46-47 -0.14223277456039796 1107 48-49 0.139445628325646 1107 50-51 0.4094451083679118 1107 52-53 0.5538960243552324 1107 54-55 0.1593561479979826 1107 56-57 0.43284258748654025 1107 58-59 0.40307529712085 1107 60-61 -0.4933021344709445 1107 62-63 -0.8152380860455217 1107 64-65 -0.028407264241536723 1107 66-67 0.8849568811702468 1107 68-69 0.4962328800360538 1107 70-71 -0.15118991090734468 1107 72-73 0.4695746040972466 1107 74-75 0.4088901815792241 1107 76 -1.191070873055807 1108 1 -0.5237710813889969 1108 2 -0.2105270004223634 1108 3 -0.4212279256770515 1108 4 -0.5007326034939403 1108 5 -0.5521943505066211 1108 6 -0.5906337690646097 1108 7 0.10656199142841416 1108 8 0.4381806257466394 1108 9 -0.307199326092352 1108 10-11 -0.41076924137028925 1108 12-13 0.2754135840999936 1108 14-15 0.22977274952901183 1108 16-17 -0.845315680285772 1108 18-19 -0.33233590782167255 1108 20-21 -0.1653535209621637 1108 22-23 -0.5845930303189419 1108 24-25 -0.7678371780512663 1108 26-27 -0.7974920061158102 1108 28-29 -0.21546855753211958 1108 30-31 -0.145235613877162 1108 32-33 -0.12827209718739851 1108 34-35 -0.21961888673830288 1108 36-37 -0.3355429583492153 1108 38-39 0.1497683128949916 1108 40-41 0.02493973250775383 1108 42-43 -0.21645410157083944 1108 44-45 -0.03534354572634868 1108 46-47 0.21569853928696148 1108 48-49 -0.19367778142409975 1108 50-51 -0.22232571837234616 1108 52-53 -0.12484177581439582 1108 54-55 0.12340541569169972 1108 56-57 0.48774973585926773 1108 58-59 -1.2529306271547611 1108 60-61 0.3604106647492813 1108 62-63 -0.07871644598323968 1108 64-65 0.014069032326688102 1108 66-67 -0.27295101406627964 1108 68-69 0.6180254797550333 1108 70-71 1.0803790094314039 1108 72-73 0.12327031406091749 1108 74-75 0.5495126102790451 1108 76 0.12668802918098976 1109 1 -0.02739509330909229 1109 2 -0.462768331836358 1109 3 0.5185781202355209 1109 4 -0.09316384828521151 1109 5 0.8120882971687067 1109 6 0.8827859344482789 1109 7 -0.21837424111445358 1109 8 0.7411581240419665 1109 9 0.8930809981746393 1109 10-11 1.366783350997821 1109 12-13 0.38198100082347963 1109 14-15 -0.0032246997726943505 1109 16-17 -0.12899878759188255 1109 18-19 0.5305261776898647 1109 20-21 0.8042148806844196 1109 22-23 0.5620973755198833 1109 24-25 0.7162651529334028 1109 26-27 0.29849380088976574 1109 28-29 0.40800039213577577 1109 30-31 0.3762369724298793 1109 32-33 1.0221307111061577 1109 34-35 0.046240900619324066 1109 36-37 0.689181136910527 1109 38-39 0.1824475562720167 1109 40-41 0.6075091190774415 1109 42-43 0.5973701780462974 1109 44-45 0.3591118423081028 1109 46-47 0.12943569109139474 1109 48-49 0.21682680225648454 1109 50-51 1.0545828858148383 1109 52-53 0.5071379196170724 1109 54-55 -0.014003984010301451 1109 56-57 0.1456594259873043 1109 58-59 0.6610590690343443 1109 60-61 0.600207871608383 1109 62-63 0.25665618325634654 1109 64-65 0.4789716764664007 1109 66-67 0.4561067428344998 1109 68-69 0.8690167164648841 1109 70-71 0.1921867221369098 1109 72-73 0.11969681101775365 1109 74-75 1.2106296140924613 1109 76 -0.23058651181959178 1110 1 0.33230769821631156 1110 2 -0.11672440005921203 1110 3 -0.5135328653067432 1110 4 -0.29814416861567494 1110 5 -0.17225240142708742 1110 6 -0.07022317145821688 1110 7 0.21944319909962218 1110 8 0.9049670169144193 1110 9 -1.1239877053053533 1110 10-11 -0.940089594977259 1110 12-13 -0.21438443050551115 1110 14-15 -0.48879294211191393 1110 16-17 0.19921653267363837 1110 18-19 0.23911443743551786 1110 20-21 -0.38187815284246085 1110 22-23 -0.13735575049494742 1110 24-25 -0.025898701424054593 1110 26-27 -0.9932978933803938 1110 28-29 0.12135269069520405 1110 30-31 0.37778926319538186 1110 32-33 0.8508136670834503 1110 34-35 0.27707433137676674 1110 36-37 0.2638924368079074 1110 38-39 0.4250595615199302 1110 40-41 0.3577981439270914 1110 42-43 0.4308269152480264 1110 44-45 1.1360723400803394 1110 46-47 0.33350347228984134 1110 48-49 0.695763710405032 1110 50-51 0.673891694253065 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2116 32-33 0.5759589909105713 2116 34-35 -0.6132089846249471 2116 36-37 0.9920863814614123 2116 38-39 0.6643931292666423 2116 40-41 0.5554983990386688 2116 42-43 0.8365254047779729 2116 44-45 2.101763198706621 2116 46-47 1.2060262340784575 2116 48-49 0.7953881502443352 2116 50-51 0.845089521689907 2116 52-53 1.3502611287861228 2116 54-55 0.2493853729299218 2116 56-57 0.23163000290215763 2116 58-59 -0.13529939651755285 2116 60-61 -0.5570074842769444 2116 62-63 0.9458570271742914 2116 64-65 1.177181914673639 2116 66-67 0.674880353292231 2116 68-69 0.7149339782932742 2116 70-71 -2.0163622236784278 2116 72-73 1.0321688348527331 2116 74-75 -1.1887060856324076 2116 76 -1.4030052205681152 2117 1 0.42923792656264936 2117 2 0.6721495642324307 2117 3 0.42324553460873915 2117 4 0.1725164208891492 2117 5 -0.4182340793878154 2117 6 -0.8851086964435027 2117 7 -1.5964836938507716 2117 8 -1.4224602005201916 2117 9 0.4411355232371861 2117 10-11 0.0777947679017501 2117 12-13 0.9862788756113972 2117 14-15 -0.1792161299052708 2117 16-17 0.42706482498109466 2117 18-19 -0.1826924114453874 2117 20-21 -0.0066800665447530605 2117 22-23 0.3552414815791849 2117 24-25 0.39356506953537007 2117 26-27 0.9062138710591512 2117 28-29 0.5553201517193642 2117 30-31 -0.38162832535863345 2117 32-33 1.2264343374829423 2117 34-35 -0.10474549687298662 2117 36-37 -0.9081860732812856 2117 38-39 0.3973916220701952 2117 40-41 -0.9148214247352868 2117 42-43 -1.0067551490753175 2117 44-45 0.6353894139872764 2117 46-47 -0.021104958262988305 2117 48-49 0.10794922482571678 2117 50-51 1.0163923356753557 2117 52-53 0.67621306412574 2117 54-55 -0.4793937979464076 2117 56-57 -0.38906579185412227 2117 58-59 -0.6880264283343038 2117 60-61 0.38384747704938604 2117 62-63 0.18741424267026474 2117 64-65 -0.2542696314449131 2117 66-67 -0.5547062442531896 2117 68-69 0.6963755868528239 2117 70-71 0.9194535671028277 2117 72-73 1.3490486069513778 2117 74-75 0.2009166183773985 2117 76 -0.26999927459531037 2118 1 0.3750046725674103 2118 2 0.44067662823722387 2118 3 -0.15080605134356162 2118 4 0.07728642137489317 2118 5 -0.0764730975384289 2118 6 0.2855410663517901 2118 7 0.2788664462052495 2118 8 -3.8238947627844055 2118 9 -1.0025668131887144 2118 10-11 -0.77555119476078 2118 12-13 0.12009714280266337 2118 14-15 -0.915323867798115 2118 16-17 -0.4134170325017834 2118 18-19 -0.9166183517637094 2118 20-21 -1.9515441393415003 2118 22-23 -0.8398006971420173 2118 24-25 -0.5656446259035022 2118 26-27 0.23325277910792153 2118 28-29 -0.3802014557675619 2118 30-31 -0.6665532779986449 2118 32-33 -0.38110521012800547 2118 34-35 0.5521253439488873 2118 36-37 -0.42719078499256113 2118 38-39 -0.3971753376616789 2118 40-41 -1.2961866876034378 2118 42-43 -0.28186808442286093 2118 44-45 -0.1294014734659683 2118 46-47 -1.2242266693751844 2118 48-49 -1.4914176895700955 2118 50-51 -0.5406865652491675 2118 52-53 -1.3282962540523968 2118 54-55 -0.3409407288574968 2118 56-57 0.2807298720761864 2118 58-59 -0.22358284743373247 2118 60-61 -1.3644645645302518 2118 62-63 -0.13082866142779181 2118 64-65 -0.3814008307454415 2118 66-67 -1.5856278614549808 2118 68-69 0.4771838639208781 2118 70-71 -0.6053800657722839 2118 72-73 -1.8563682418690703 2118 74-75 -0.2904573878725003 2118 76 -1.2052091967144456 2119 1 0.17938624924500246 2119 2 0.2917654508630463 2119 3 0.2823983154471428 2119 4 0.16984661260681122 2119 5 0.5033752640936129 2119 6 0.5300846315784611 2119 7 -0.841351729041385 2119 8 0.7544421438257487 2119 9 0.08496614720952067 2119 10-11 0.2479420735142739 2119 12-13 0.6240113184435927 2119 14-15 0.12575649396536193 2119 16-17 0.07482811893122232 2119 18-19 -0.33623605221632147 2119 20-21 -0.8111102531770626 2119 22-23 1.1249429715864778 2119 24-25 -0.020140793872158724 2119 26-27 0.29577280177974075 2119 28-29 0.565825635420822 2119 30-31 0.43871052840367497 2119 32-33 0.19963030988644448 2119 34-35 0.5142855746100707 2119 36-37 -0.10122168767621531 2119 38-39 0.1291436943499562 2119 40-41 0.14362618769330737 2119 42-43 -0.2711975405454936 2119 44-45 -0.6943488600452312 2119 46-47 0.17552854062670775 2119 48-49 -0.3061083752285185 2119 50-51 0.6360633135879965 2119 52-53 -0.5434485906468538 2119 54-55 -0.5519447785712259 2119 56-57 0.5893172366407811 2119 58-59 1.1550736004826803 2119 60-61 0.8015326261348719 2119 62-63 0.2915367656814638 2119 64-65 0.291299334364588 2119 66-67 -0.08317996147387419 2119 68-69 -0.386600359445886 2119 70-71 -0.6533728699320704 2119 72-73 -1.5522659557933487 2119 74-75 -0.5836468044015781 2119 76 0.42804117088127214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 6.0 12 42.0 13 239.0 14 828.0 15 1599.0 16 2253.0 17 2715.0 18 2935.0 19 2840.0 20 2535.0 21 1945.0 22 1652.0 23 1452.0 24 1397.0 25 1442.0 26 1473.0 27 1837.0 28 2102.0 29 2601.0 30 3307.0 31 4185.0 32 5252.0 33 7095.0 34 9820.0 35 14904.0 36 26456.0 37 36824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.44624124928804 26.35847818720576 10.489318904421678 27.705961659084522 2 17.918661485504913 26.555600878793733 32.29352283217759 23.232214803523764 3 16.54536736869977 25.590215905594082 28.39262328517143 29.47179344053472 4 13.944052040619162 20.696737442481233 36.24737900484482 29.111831512054785 5 11.23825472136943 39.48846762131719 33.891524793004 15.38175286430938 6 23.87556624229803 41.702627078010835 17.651731466970094 16.77007521272104 7 23.371762668441427 32.50391807466884 23.503438602517587 20.620880654372144 8 20.882085632294952 35.92963925087844 21.528299591375227 21.659975525451383 9 21.89684908077317 19.651917530789984 21.454589693495638 36.99664369494121 10-11 22.414249626083286 26.549160208105228 27.386447397611224 23.65014276820026 12-13 22.20993723924229 24.4763376915205 26.423209314641078 26.890515754596134 14-15 20.967603426436806 26.051081674860633 23.619370674910726 29.361944223791838 16-17 21.652103594609876 28.242698784144498 25.120762575409522 24.984435045836108 18-19 20.745400287683292 27.245110457502307 25.9394433829265 26.070045871887903 20-21 20.248395199553446 27.7814751998397 25.727259065243995 26.24287053536286 22-23 21.96769645834675 27.308443719272635 25.895789948259946 24.82806987412067 24-25 20.923234361693755 28.514638213214827 25.48000887381295 25.08211855127847 26-27 19.97502451033012 29.083921939071256 26.524470970465945 24.41658258013268 28-29 20.92895940230576 27.71420597264862 27.93676692644038 23.420067698605237 30-31 21.017339716753618 27.48341527297709 27.577162812998708 23.922082197270587 32-33 19.973235435138868 29.089646979683266 26.634320187208825 24.30279739796904 34-35 21.201614461452586 28.26595676163078 26.678331436913634 23.854097340003005 36-37 21.267810243528913 27.453000994725805 26.75239914983147 24.52678961191381 38-39 20.506379842132006 27.436183687928033 27.95716238362066 24.100274086319303 40-41 20.490278165410736 26.65364219927435 27.944281042243645 24.911798593071268 42-43 19.59037334421091 26.53592105168996 28.474205113892527 25.3995004902066 44-45 19.324516770790844 26.874414077874864 28.476709819160277 25.324359332174012 46-47 19.853009582286724 27.191438201764743 28.097783693653078 24.857768522295455 48-49 19.168151599075404 27.927463735445873 27.97469532049493 24.929689344983792 50-51 19.392501628058422 27.59290667468172 27.30593901400488 25.708652683254975 52-53 18.81999756685774 27.447633769152052 27.747124956167657 25.98524370782255 54-55 18.398133636760488 27.85053350222203 27.61365994690025 26.137672914117232 56-57 19.115552788452593 27.358537824627692 27.485562163206595 26.040347223713116 58-59 19.236852086419486 27.325618841108657 27.058688822573835 26.378840249898023 60-61 18.967417362616917 26.856523325962346 27.153509807710197 27.02254950371054 62-63 18.715515575688617 26.93595826445394 27.091965621131127 27.256560538726323 64-65 19.384271882178663 27.518838961763887 26.107974265942445 26.988914890115 66-67 19.14704051181863 28.059139669522033 25.685752520806943 27.108067297852394 68-69 18.81856630670474 28.530739889936097 25.53260768443576 27.118086118923408 70-71 19.165646893807654 28.65382826309424 25.29716538926698 26.883359453831122 72-73 19.068679018441788 28.815560660383433 24.905715737421012 27.210044583753767 74-75 19.484102277850536 29.267123238655472 24.793719630448628 26.45505485304536 76 19.510222775642816 28.404073366395444 25.334020338206777 26.75168351975497 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 3.5 11 2.5 12 2.0 13 3.5 14 6.5 15 11.0 16 19.0 17 24.0 18 24.0 19 29.0 20 42.0 21 50.0 22 60.0 23 119.5 24 180.0 25 191.0 26 279.5 27 464.0 28 675.5 29 791.0 30 965.5 31 1338.0 32 1843.5 33 2151.0 34 2443.5 35 3169.5 36 3948.0 37 4293.0 38 4906.0 39 6168.0 40 7604.0 41 8773.0 42 9155.0 43 9460.5 44 9974.5 45 10289.5 46 10396.0 47 10321.5 48 9926.5 49 9005.5 50 8405.0 51 7871.5 52 6680.5 53 5583.5 54 5144.0 55 4672.0 56 3605.5 57 2701.0 58 2391.0 59 1991.0 60 1382.5 61 989.5 62 805.0 63 677.0 64 465.5 65 301.0 66 217.0 67 214.0 68 154.5 69 85.5 70 62.5 71 49.0 72 44.5 73 37.0 74 27.5 75 21.0 76 14.5 77 8.5 78 10.0 79 11.0 80 13.5 81 11.5 82 7.0 83 7.0 84 7.0 85 5.0 86 3.5 87 4.0 88 5.0 89 6.0 90 4.5 91 3.0 92 3.0 93 3.0 94 3.5 95 4.0 96 4.0 97 4.5 98 4.0 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7399614991018842 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 139737.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25522946678403 #Duplication Level Percentage of deduplicated Percentage of total 1 78.48779982386156 43.368613896104826 2 8.778428223592186 9.701081317045594 3 4.123711340206185 6.835698490736168 4 2.2056157074029947 4.874872081123825 5 1.3961560379215665 3.8572461123396096 6 0.9493343003678184 3.147341076450761 7 0.751178573278765 2.905458110593472 8 0.5348909495933274 2.3644417727588256 9 0.44552660208257777 2.2155907168466475 >10 2.313111951510128 20.239449823597187 >50 0.014246490182873129 0.49020660240308583 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005725040612006842 0.0 2 0.0 0.0 0.0 0.005725040612006842 0.0 3 0.0 0.0 0.0 0.006440670688507697 0.0 4 0.0 0.0 0.0 0.006440670688507697 0.0 5 0.0 0.0 0.0 0.007156300765008552 0.0 6 0.0 0.0 0.0 0.007156300765008552 0.0 7 0.0 0.0 0.0 0.007156300765008552 0.0 8 0.0 0.0 0.0 0.007156300765008552 0.0 9 0.0 0.0 0.0 0.010734451147512827 0.0 10 0.0 0.0 0.0 0.012881341377015394 0.0 11 0.0 0.0 0.0 0.013596971453516248 0.0 12 0.0 0.0 0.0 0.014312601530017104 0.0 13 0.0 0.0 0.0 0.017890751912521378 0.0 14 0.0 0.0 0.0 0.021468902295025655 0.0 15 0.0 0.0 0.0 0.024331422601029076 0.0 16 0.0 0.0 0.0 0.031487723366037626 0.0 17 0.0 0.0 0.0 0.03435024367204105 0.0 18 0.0 0.0 0.0 0.04436906474305302 0.0 19 0.0 0.0 0.0 0.05295662566106328 0.0 20 0.0 0.0 0.0 0.05868166627307012 0.0 21 0.0 0.0 0.0 0.06082855650257269 0.0 22 0.0 0.0 0.0 0.06154418657907355 0.0 23 0.0 0.0 0.0 0.06369107680857611 0.0 24 0.0 0.0 0.0 0.06512233696157782 0.0 25 0.0 0.0 0.0 0.06512233696157782 0.0 26 0.0 0.0 0.0 0.07013174749708381 0.0 27 0.0 0.0 0.0 0.08229745879759835 0.0 28 0.0 0.0 0.0 0.1023351009396223 0.0 29 0.0 0.0 0.0 0.12022585285214367 0.0 30 0.0 0.0 0.0 0.14598853560617445 0.0 31 0.0 0.0 0.0 0.1774762589722121 0.0 32 0.0 0.0 0.0 0.20395457180274373 0.0 33 0.0 0.0 0.0 0.23973607562778648 0.0 34 0.0 0.0 0.0 0.3041427825128635 0.0 35 0.0 0.0 0.0 0.3792839405454532 0.0 36 0.0 0.0 0.0 0.4966472730915935 0.0 37 0.0 0.0 0.0 0.755705360784903 0.0 38 0.0 0.0 0.0 1.033369830467235 0.0 39 0.0 0.0 0.0 1.383312937876153 0.0 40 0.0 0.0 0.0 1.8148378740061688 0.0 41 0.0 0.0 0.0 2.2549503710541945 0.0 42 0.0 0.0 0.0 2.7888104081238327 0.0 43 0.0 0.0 0.0 3.2990546526689424 0.0 44 0.0 0.0 0.0 3.7656454625475 0.0 45 0.0 0.0 0.0 4.295211719158133 0.0 46 0.0 0.0 0.0 4.806887223856244 0.0 47 0.0 0.0 0.0 5.326434659395865 0.0 48 0.0 0.0 0.0 5.800897400115932 0.0 49 0.0 0.0 0.0 6.307563494278537 0.0 50 0.0 0.0 0.0 6.81995462905315 0.0 51 0.0 0.0 0.0 7.3309145036747605 0.0 52 0.0 0.0 0.0 7.808955394777332 0.0 53 0.0 0.0 0.0 8.297730737027416 0.0 54 0.0 0.0 0.0 8.800103050731016 0.0 55 0.0 0.0 0.0 9.320366116347138 0.0 56 0.0 0.0 0.0 9.819160279668234 0.0 57 0.0 0.0 0.0 10.260704036869262 0.0 58 0.0 0.0 0.0 10.723716696365315 0.0 59 0.0 0.0 0.0 11.178141794943357 0.0 60 0.0 0.0 0.0 11.633998153674403 0.0 61 0.0 0.0 0.0 12.093432662787952 0.0 62 0.0 0.0 0.0 12.600814387027057 0.0 63 0.0 0.0 0.0 13.050230075069596 0.0 64 0.0 0.0 0.0 13.458854848751583 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGAG 20 0.0069429744 52.49302 30 GACCCAT 20 0.0069429744 52.49302 47 GCCGTTG 20 0.0069429744 52.49302 28 ACGCTTC 35 0.0012528052 39.994682 15 GACGATA 40 0.0024137641 34.995346 43 GCTCTAA 60 4.701307E-4 29.436718 1 AGATACC 60 4.9660413E-4 29.16279 27 TAGGCCA 60 4.9660413E-4 29.16279 5 CTATAGG 50 0.007193712 27.996279 2 ATGTCTG 50 0.007193712 27.996279 33 GTGACGA 50 0.007193712 27.996279 41 CACAGCT 65 7.9303526E-4 26.9195 17 GAACAGT 70 0.0012221142 24.996677 29 TGCCGCC 80 0.0026576004 21.872091 19 CCACAGC 100 3.913335E-4 20.99721 16 CTTTACA 105 5.1060144E-4 20.185177 1 TAGGGTT 125 7.771679E-5 19.597395 4 CATGGGC 90 0.0052581932 19.441858 33 AAAGAAA 95 0.006807582 18.59161 1 GCATAGT 95 0.006807582 18.59161 1 >>END_MODULE