FastQCFastQC Report
Wed 25 May 2016
SRR1780424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139737
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT42233.0221058130631118No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT39672.8389045134788926No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC15151.0841795658987956No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC7000.5009410535505987No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG3860.2762332095293301No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT3530.25261741700480184No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC2590.1853481898137215No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC2020.14455727545317273No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1790.12809778369365307No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1450.10376636109262401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTA207.9043006E-570.026
GGCGAAG207.9043006E-570.043
TAGGGCG252.3837485E-455.99999640
CCTGCGG252.3837485E-455.99999653
GTAATCA200.00693932552.54
TGCGATG200.00693932552.528
ACATGTA200.00693932552.534
TTTGAAC200.00693932552.531
CTTAGGA200.00693932552.527
CTATACA1550.051.9354861
GGAGTAA352.0779818E-550.01
TTTACAC1900.049.736842
AACCGCC305.861588E-446.66666419
TGTATTC305.861588E-446.66666421
GATCACA305.861588E-446.6666641
GCGGCGT305.861588E-446.66666456
CTTTATA551.3015597E-744.5454522
TTTATAC757.4760464E-1042.03
CGCCTTT350.001251986640.022
GTTACTT350.001251986640.046