##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139737 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89572554155306 34.0 34.0 34.0 31.0 34.0 2 33.27731381094485 34.0 34.0 34.0 32.0 34.0 3 33.49172373816527 34.0 34.0 34.0 34.0 34.0 4 33.544186579073546 34.0 34.0 34.0 34.0 34.0 5 33.55436999506215 34.0 34.0 34.0 34.0 34.0 6 37.27816541073588 38.0 38.0 38.0 36.0 38.0 7 36.964247121378015 38.0 37.0 38.0 35.0 38.0 8 36.95612472000973 38.0 37.0 38.0 35.0 38.0 9 36.74935772200634 38.0 37.0 38.0 34.0 38.0 10-11 36.49688343101684 38.0 37.0 38.0 34.0 38.0 12-13 36.374124247693885 38.0 37.0 38.0 34.0 38.0 14-15 36.57135905307828 38.0 37.0 38.0 34.0 38.0 16-17 36.677805448807405 38.0 37.0 38.0 34.0 38.0 18-19 36.712338178148954 38.0 37.0 38.0 34.0 38.0 20-21 36.47644145788159 38.0 37.0 38.0 34.0 38.0 22-23 36.52783085367511 38.0 37.0 38.0 34.0 38.0 24-25 35.89979747668835 38.0 36.5 38.0 32.5 38.0 26-27 36.1099887646078 38.0 37.0 38.0 33.5 38.0 28-29 35.99842919198208 38.0 37.0 38.0 33.0 38.0 30-31 35.70567566213673 38.0 36.5 38.0 31.5 38.0 32-33 36.1544508612608 38.0 37.0 38.0 34.0 38.0 34-35 36.06621367282824 38.0 37.0 38.0 33.5 38.0 36-37 36.13629890437035 38.0 37.0 38.0 34.0 38.0 38-39 35.73588598581621 38.0 36.5 38.0 32.5 38.0 40-41 35.49888361708066 38.0 36.0 38.0 31.0 38.0 42-43 35.92787880089024 38.0 36.5 38.0 32.5 38.0 44-45 36.13731509907898 38.0 37.0 38.0 34.0 38.0 46-47 36.36606983118287 38.0 37.0 38.0 34.0 38.0 48-49 36.15778211926691 38.0 37.0 38.0 33.5 38.0 50-51 36.2672198487158 38.0 37.0 38.0 34.0 38.0 52-53 36.17599132656348 38.0 37.0 38.0 34.0 38.0 54-55 36.35780788194967 38.0 37.0 38.0 34.0 38.0 56-57 36.36688564947008 38.0 37.0 38.0 34.0 38.0 58-59 36.40977693810515 38.0 37.0 38.0 34.0 38.0 60-61 36.14120454854476 38.0 37.0 38.0 33.0 38.0 62-63 36.43775449594595 38.0 37.0 38.0 34.0 38.0 64-65 36.42710234225724 38.0 37.0 38.0 34.0 38.0 66-67 35.70830560266787 38.0 36.5 38.0 31.0 38.0 68-69 34.7539270200448 38.0 35.5 38.0 26.0 38.0 70-71 34.325733341920895 38.0 34.5 38.0 24.0 38.0 72-73 33.726911984656894 38.0 34.0 38.0 22.0 38.0 74-75 34.17312522810708 38.0 35.0 38.0 23.0 38.0 76 33.75609895732698 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28012752174217326 1101 2 0.4558900831765058 1101 3 0.16286940367304936 1101 4 0.23482586383492077 1101 5 0.20851155838345647 1101 6 -1.013799637143137 1101 7 -0.14591273652538916 1101 8 0.3366943698199023 1101 9 -0.004159658176760672 1101 10-11 -0.03249085470433499 1101 12-13 0.5802755839106553 1101 14-15 0.5168830984282096 1101 16-17 0.09506817204362505 1101 18-19 -0.44792282609239464 1101 20-21 -0.07221451697954961 1101 22-23 0.5202715126143005 1101 24-25 -1.6588304180049107 1101 26-27 -0.23126332219464274 1101 28-29 0.4534073730666961 1101 30-31 1.0886186286256816 1101 32-33 0.10710706752603727 1101 34-35 0.23002369966177838 1101 36-37 0.02842783143142924 1101 38-39 0.5770730081540023 1101 40-41 1.0911249584034906 1101 42-43 0.7819518539527266 1101 44-45 0.43795796476258886 1101 46-47 0.6480512149302626 1101 48-49 -0.9236141079799296 1101 50-51 -1.3214180545313141 1101 52-53 -1.8015799753188233 1101 54-55 -1.190502876331223 1101 56-57 -1.3216246695486404 1101 58-59 -1.1207033252936753 1101 60-61 -1.3113030294035752 1101 62-63 -0.4647064180309286 1101 64-65 -1.129623389112517 1101 66-67 -1.6184981324906715 1101 68-69 -1.1761449469432605 1101 70-71 -0.9528125946898669 1101 72-73 -1.3770926741051568 1101 74-75 -0.5873257443298172 1101 76 -0.9526878638665224 1102 1 -1.1306811463339344 1102 2 -0.06868708384252642 1102 3 0.24406654955042484 1102 4 0.279223326837041 1102 5 0.1493810087005798 1102 6 0.3356321810386831 1102 7 -0.05136992468606394 1102 8 0.379109803223713 1102 9 0.6072964729014672 1102 10-11 0.4938701389531701 1102 12-13 0.6664278037838045 1102 14-15 -0.13553497768172207 1102 16-17 0.35623360544742866 1102 18-19 -0.1888715575934583 1102 20-21 0.5842336859802799 1102 22-23 0.5100640601830122 1102 24-25 -4.484246644220555 1102 26-27 -1.1228463031671652 1102 28-29 0.27297661196733003 1102 30-31 1.3858239668920618 1102 32-33 0.1209482408875644 1102 34-35 0.6389164057928625 1102 36-37 -2.337322697109798 1102 38-39 0.20735842041616337 1102 40-41 1.2501167660144858 1102 42-43 0.865791705961179 1102 44-45 0.9325734510205166 1102 46-47 1.0695958766638824 1102 48-49 -3.092416962102554 1102 50-51 -0.5119571665820573 1102 52-53 -0.05039075755983191 1102 54-55 0.6778903583410738 1102 56-57 0.43085286744924645 1102 58-59 -0.023121401403614072 1102 60-61 -5.181711327500828 1102 62-63 -2.1566990184537573 1102 64-65 -0.09282370623724745 1102 66-67 0.7129963178264589 1102 68-69 -0.6970223253787751 1102 70-71 -1.4484851634002283 1102 72-73 -5.587716352752093 1102 74-75 -2.1428556069090945 1102 76 -0.6529389209489764 1103 1 0.48155617664459527 1103 2 0.45552488509306244 1103 3 -0.03958180586300841 1103 4 0.24233433643045998 1103 5 0.32598847786458407 1103 6 0.17338629250510706 1103 7 0.5792976175706741 1103 8 0.5603854998888522 1103 9 0.4256638781093187 1103 10-11 0.2724933687384592 1103 12-13 -0.6733106612687294 1103 14-15 -0.1700398885999519 1103 16-17 0.042377392564837635 1103 18-19 0.4773430381123447 1103 20-21 -0.2538202199128179 1103 22-23 0.49136973629082803 1103 24-25 -2.258865477020919 1103 26-27 -0.35978383171900674 1103 28-29 -0.11243053741594622 1103 30-31 0.9398369294320332 1103 32-33 0.8926262331214616 1103 34-35 0.5014535231980659 1103 36-37 -0.9351984394578992 1103 38-39 -0.2456452342206603 1103 40-41 -0.26719279314495026 1103 42-43 -0.396556969232968 1103 44-45 0.7880368475486108 1103 46-47 0.8166193462847104 1103 48-49 -1.860675870279806 1103 50-51 -0.575741999839245 1103 52-53 -0.08405338889834724 1103 54-55 -0.4105347216041082 1103 56-57 0.3081156015744213 1103 58-59 -0.11572932739721864 1103 60-61 -4.266303321562045 1103 62-63 -1.2528330979421156 1103 64-65 0.08943702925841279 1103 66-67 -0.36251618788391937 1103 68-69 0.3151122109391764 1103 70-71 0.7077576986372307 1103 72-73 -4.097738052158213 1103 74-75 -1.8754889440493443 1103 76 -2.307076084037142 1104 1 -0.08024768803964832 1104 2 0.1335153528959978 1104 3 0.1943067088756365 1104 4 -0.26271669190710156 1104 5 -0.3816346895187266 1104 6 0.1511488164651098 1104 7 0.5423214998687627 1104 8 0.5135404498966238 1104 9 0.8058379443166501 1104 10-11 -0.5555968244957512 1104 12-13 0.21568975317461536 1104 14-15 0.43033311041574507 1104 16-17 0.1417596129450942 1104 18-19 0.6062608229126312 1104 20-21 -0.1640090506645251 1104 22-23 0.27475185306023775 1104 24-25 0.330200989331459 1104 26-27 0.9656319067672356 1104 28-29 0.960330173354393 1104 30-31 0.6598545432744487 1104 32-33 0.6735929222371411 1104 34-35 9.419446869500803E-4 1104 36-37 0.8851468436587098 1104 38-39 1.2207136219156922 1104 40-41 1.5642129468954593 1104 42-43 0.5211630742835851 1104 44-45 0.6346733807299785 1104 46-47 0.7385421044052123 1104 48-49 -0.15570773061239152 1104 50-51 0.24331462348354904 1104 52-53 0.5548693174167312 1104 54-55 0.09160872760068628 1104 56-57 0.018057061451123957 1104 58-59 -0.7091863029968621 1104 60-61 0.4654741223585859 1104 62-63 0.40076349326133 1104 64-65 0.45090286358468035 1104 66-67 -0.04562891647533007 1104 68-69 1.3891421544712728 1104 70-71 0.8722593305079229 1104 72-73 0.47982113553281636 1104 74-75 -0.3721345431790155 1104 76 -0.808837468277936 1105 1 0.6316042377734874 1105 2 0.1877513929349348 1105 3 0.15866730896050996 1105 4 0.007321499850462487 1105 5 -0.16230042427321933 1105 6 0.1260308713314373 1105 7 0.5515012507486716 1105 8 -0.6266270446741515 1105 9 0.6550553877154641 1105 10-11 0.279632575668991 1105 12-13 -0.3528463542615441 1105 14-15 0.2454151103658262 1105 16-17 0.02641988103624726 1105 18-19 0.5238802033574572 1105 20-21 0.3193756626476798 1105 22-23 0.23427919885789805 1105 24-25 0.344435531654419 1105 26-27 0.34871021235121447 1105 28-29 0.6548897200456949 1105 30-31 1.0214343840173115 1105 32-33 0.7478983564499657 1105 34-35 -0.8991572789969311 1105 36-37 0.02136099208237141 1105 38-39 -0.14436400969609764 1105 40-41 0.1731749049094944 1105 42-43 0.33682543917712593 1105 44-45 0.7735272535738886 1105 46-47 0.860665241620957 1105 48-49 -0.06658738913314721 1105 50-51 0.809388860272449 1105 52-53 0.30552768840097855 1105 54-55 0.38184424343903345 1105 56-57 0.3512489438304982 1105 58-59 0.30410592635337963 1105 60-61 0.5093759221139607 1105 62-63 -0.4438578342142705 1105 64-65 0.5370808172054069 1105 66-67 0.3906838315838783 1105 68-69 -0.11245540924406328 1105 70-71 0.7997917186160635 1105 72-73 0.7651204291101124 1105 74-75 -0.050312821304871136 1105 76 0.496858844844553 1106 1 -0.704387391612201 1106 2 0.20932852935314372 1106 3 0.36643024887049336 1106 4 -0.018749475983412367 1106 5 0.19921889588265174 1106 6 0.22373498889564303 1106 7 -0.4015414809691151 1106 8 0.15553576645714884 1106 9 -0.985477702369252 1106 10-11 0.11458123187686198 1106 12-13 1.0546643940633302 1106 14-15 -0.437088427694313 1106 16-17 0.12997605067533868 1106 18-19 0.4305961457558283 1106 20-21 0.08643716117544642 1106 22-23 0.2743984532829913 1106 24-25 -0.5143673631830339 1106 26-27 0.738185238006345 1106 28-29 0.5781657960519411 1106 30-31 0.7312698558369135 1106 32-33 0.0751907175936779 1106 34-35 0.6984495838336571 1106 36-37 0.9603353235399155 1106 38-39 0.25145940253653976 1106 40-41 0.9190013353924726 1106 42-43 0.604215591632304 1106 44-45 0.9508662299175157 1106 46-47 0.7638833672204157 1106 48-49 0.8836517233671017 1106 50-51 0.09541503185662492 1106 52-53 0.7040004537219531 1106 54-55 0.09688116670169222 1106 56-57 0.25795482539435 1106 58-59 0.657492738611495 1106 60-61 1.0236958432370216 1106 62-63 0.8258463101788323 1106 64-65 -0.7898694755648776 1106 66-67 0.32563718284786347 1106 68-69 1.1571548340269118 1106 70-71 -0.08390838802878164 1106 72-73 0.22509842941864378 1106 74-75 -0.25674773105918547 1106 76 0.3335647934806403 1107 1 0.5882743059444593 1107 2 -0.2879117494512329 1107 3 0.11147898437246084 1107 4 0.07173069478081828 1107 5 0.13264019029205798 1107 6 0.24991607358005297 1107 7 0.5850433036068381 1107 8 0.10540486103373325 1107 9 -0.5230948782660718 1107 10-11 0.4151110707146799 1107 12-13 -0.22051676660839803 1107 14-15 0.08885433313214719 1107 16-17 0.5582923789681757 1107 18-19 0.6008940851179432 1107 20-21 0.5802096551482663 1107 22-23 0.059609939802143685 1107 24-25 0.2861037581825343 1107 26-27 0.016329904042088117 1107 28-29 0.6142045081359981 1107 30-31 0.5027794475087006 1107 32-33 0.9732882714928692 1107 34-35 0.37903344274592854 1107 36-37 1.0488709097820603 1107 38-39 0.30589049930983947 1107 40-41 0.30606000450915616 1107 42-43 0.6694714271128461 1107 44-45 0.43966155462513967 1107 46-47 0.6124751840771694 1107 48-49 0.7006500861858171 1107 50-51 0.8260330850615674 1107 52-53 0.2996857557024626 1107 54-55 0.49682314002323125 1107 56-57 0.683583965839631 1107 58-59 0.4009250166421836 1107 60-61 0.7124276149158533 1107 62-63 0.5177937269554462 1107 64-65 0.6457347272245428 1107 66-67 -0.27993683225289345 1107 68-69 0.8265409255847231 1107 70-71 0.17384749926130638 1107 72-73 0.740596388122988 1107 74-75 -0.5234701217583364 1107 76 0.2365773452038269 1108 1 -0.1844132644696046 1108 2 0.38401250083675365 1108 3 0.076914380653335 1108 4 0.30125839836449586 1108 5 -0.11214125710467471 1108 6 -0.19273364729541953 1108 7 -0.11547063996064111 1108 8 -0.5870011797019927 1108 9 -0.27585131205469793 1108 10-11 0.3298040337385899 1108 12-13 0.9385494004612411 1108 14-15 0.510360846037706 1108 16-17 -0.4149217937219305 1108 18-19 0.0829472010590564 1108 20-21 0.8038922000550386 1108 22-23 0.09588979853096191 1108 24-25 0.9191119736843802 1108 26-27 -0.3067266677264584 1108 28-29 -0.7038202269805254 1108 30-31 0.39883879153442336 1108 32-33 0.6342659035548408 1108 34-35 0.32282561206881866 1108 36-37 0.995049245668234 1108 38-39 1.0418646502269624 1108 40-41 0.9823863206983745 1108 42-43 -0.25688653584776944 1108 44-45 0.7222957052890848 1108 46-47 0.7734274987220289 1108 48-49 0.958387721792235 1108 50-51 0.6743026396016134 1108 52-53 0.06102479389378601 1108 54-55 -0.32415740354171163 1108 56-57 0.03167629845986397 1108 58-59 0.34644193500616893 1108 60-61 1.0328638340127867 1108 62-63 0.8071623105725507 1108 64-65 0.6171007284650969 1108 66-67 0.504694229686173 1108 68-69 0.6232072546858305 1108 70-71 -0.8579005418035521 1108 72-73 -0.7920599152809373 1108 74-75 -0.651832591219204 1108 76 0.31811841638374005 1109 1 -1.1816479034574066 1109 2 0.10770368956317355 1109 3 0.3163895934219738 1109 4 0.11741871353989097 1109 5 0.15838428775983004 1109 6 -0.012555856048606984 1109 7 0.09606053664364822 1109 8 0.47598338983610233 1109 9 0.7674474226979342 1109 10-11 0.4700545577681865 1109 12-13 0.7058613180307489 1109 14-15 0.7464330232228491 1109 16-17 0.7903273413135565 1109 18-19 0.7320494057715337 1109 20-21 1.117476562506738 1109 22-23 0.5866908693191561 1109 24-25 0.08162428522313547 1109 26-27 0.5310009379002238 1109 28-29 -0.2741033292357429 1109 30-31 0.5173326168242198 1109 32-33 0.8448500387165936 1109 34-35 0.3929445604739996 1109 36-37 1.1592851726324014 1109 38-39 1.1541585108729677 1109 40-41 0.6884435977648238 1109 42-43 1.0971491234193493 1109 44-45 1.0614249322507305 1109 46-47 1.0318372267317244 1109 48-49 0.9547195146048182 1109 50-51 0.5672490749376209 1109 52-53 -0.0421648416846665 1109 54-55 -0.08350154577470903 1109 56-57 0.4422377046269901 1109 58-59 0.3877645099487168 1109 60-61 0.9666025010835213 1109 62-63 0.6149823474440268 1109 64-65 0.9404333041652819 1109 66-67 0.7054417610648116 1109 68-69 0.3390190586103685 1109 70-71 0.48977099896973897 1109 72-73 0.5031644704048475 1109 74-75 0.00978318775395337 1109 76 0.7567880125652735 1110 1 -0.7324643174708925 1110 2 -0.5507663622664509 1110 3 -0.05231518555520154 1110 4 -0.06299124710077564 1110 5 0.3172765107181448 1110 6 0.08084788243910879 1110 7 0.49438873897403113 1110 8 0.48789511060562063 1110 9 0.7762155922938234 1110 10-11 -0.6634034784216212 1110 12-13 0.533342710026389 1110 14-15 0.8414929715231239 1110 16-17 0.4303420198789496 1110 18-19 -0.15639024973682325 1110 20-21 0.6881382670389016 1110 22-23 -0.41939164240421434 1110 24-25 -0.9420595150964175 1110 26-27 0.9114745489572087 1110 28-29 0.5614512737285509 1110 30-31 -0.1481519706821075 1110 32-33 0.7419019852502515 1110 34-35 0.8511983307869215 1110 36-37 1.303550789716688 1110 38-39 0.8044092155740117 1110 40-41 -1.1689469949634415 1110 42-43 -0.11427742529919982 1110 44-45 0.5282260441845281 1110 46-47 0.24823565235816147 1110 48-49 1.1211556108858147 1110 50-51 -0.21036091687874858 1110 52-53 0.6015941700451961 1110 54-55 -0.18012352725114056 1110 56-57 0.6558595432147314 1110 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36-37 0.134544976763614 2116 38-39 -3.4726038093311686 2116 40-41 -0.7722971112973127 2116 42-43 -1.2221462336064164 2116 44-45 -0.17736321868513727 2116 46-47 -0.4314908321144486 2116 48-49 -0.0015485718967696016 2116 50-51 -0.8111561767409441 2116 52-53 -0.1615036775910923 2116 54-55 -0.38252576564954666 2116 56-57 -0.33044971785942323 2116 58-59 -1.956933917683891 2116 60-61 -1.1995260253388125 2116 62-63 0.1506564126480896 2116 64-65 -1.6275576864404329 2116 66-67 -0.4414099403136831 2116 68-69 -0.6371922915157882 2116 70-71 1.1722290525414252 2116 72-73 0.731360880902507 2116 74-75 0.6380565772534439 2116 76 -0.4954678998497286 2117 1 0.7645172246592367 2117 2 0.6049860638190623 2117 3 0.26011029626613436 2117 4 -0.006772469274366699 2117 5 0.20720089834851052 2117 6 0.29978417788576195 2117 7 0.5428727185088889 2117 8 0.5721930927665397 2117 9 -0.7019685034516598 2117 10-11 -0.548185168456186 2117 12-13 -1.1185498630580213 2117 14-15 0.1060079808048755 2117 16-17 -0.48879726685945 2117 18-19 -0.7657074745666534 2117 20-21 0.3577491878194863 2117 22-23 0.2900742414757076 2117 24-25 -1.1328524639788355 2117 26-27 -1.256434716199216 2117 28-29 -0.6473958262880615 2117 30-31 -0.14829157435859486 2117 32-33 -1.6039680097692965 2117 34-35 0.6548287809899094 2117 36-37 -0.7741329966368582 2117 38-39 0.4772612054923542 2117 40-41 0.4722413718999334 2117 42-43 0.253419793191874 2117 44-45 0.2483659497067947 2117 46-47 -0.34300100983112713 2117 48-49 -0.43511598995331724 2117 50-51 -0.09675748187370203 2117 52-53 0.8179421378684353 2117 54-55 -0.06393176462266581 2117 56-57 0.5959129699190342 2117 58-59 -0.5384426047667219 2117 60-61 -0.2494415560872767 2117 62-63 -0.11180280195350178 2117 64-65 -0.5328361865315259 2117 66-67 -0.3224822029302459 2117 68-69 0.3528660088303681 2117 70-71 -0.00987671621157915 2117 72-73 1.2828498584933072 2117 74-75 0.9816435391760336 2117 76 0.971092214100949 2118 1 0.4128794597266747 2118 2 0.3013154626306118 2118 3 -0.6517110796830892 2118 4 0.16380443200637984 2118 5 0.12882619586635968 2118 6 0.07233481663837438 2118 7 0.6190130012115631 2118 8 0.5457605544332864 2118 9 -1.557022663844144 2118 10-11 -0.566234584754497 2118 12-13 -1.1988796828472132 2118 14-15 0.4971123994509483 2118 16-17 -2.2532886125306675 2118 18-19 -0.40996112978596955 2118 20-21 -0.2074011497722026 2118 22-23 0.46575144877481023 2118 24-25 0.5195292979745076 2118 26-27 -2.4920769499713415 2118 28-29 -0.15921558309251083 2118 30-31 -1.0404039106225724 2118 32-33 -0.3563602961636221 2118 34-35 -0.08752075998254583 2118 36-37 -3.1024105504329427 2118 38-39 -0.7351867794819782 2118 40-41 -0.48553942828141317 2118 42-43 0.09030310142846076 2118 44-45 -2.2094998555748475 2118 46-47 -0.28857750632568013 2118 48-49 0.09286283163312703 2118 50-51 -1.39561517524006 2118 52-53 -1.6746745619906989 2118 54-55 -0.9589666571924056 2118 56-57 -0.12752900802821898 2118 58-59 -0.7131280858955975 2118 60-61 -0.74792360786752 2118 62-63 -1.1798878394367023 2118 64-65 0.017924001936926004 2118 66-67 0.11256580471211919 2118 68-69 -1.6320395140724315 2118 70-71 -0.7464742453966622 2118 72-73 0.2663548211720226 2118 74-75 -0.5099814345128664 2118 76 -0.2577819945420501 2119 1 0.6308657744871837 2119 2 -1.142000866833726 2119 3 0.007537957175060228 2119 4 -0.2039145030749836 2119 5 -0.08128248103548685 2119 6 -0.6831544758528167 2119 7 -0.6781111270321105 2119 8 0.028120067153331263 2119 9 -0.10338901096950082 2119 10-11 -0.025466371310763236 2119 12-13 -0.15670269474992438 2119 14-15 -0.26099318882541667 2119 16-17 -0.18124806610681787 2119 18-19 -0.21572273061398306 2119 20-21 -1.1686988653393726 2119 22-23 -0.4541496494944042 2119 24-25 0.9138013763073047 2119 26-27 0.6633523771847436 2119 28-29 -0.05446107131712097 2119 30-31 0.9557928085342979 2119 32-33 0.3147257335561804 2119 34-35 0.505027109605173 2119 36-37 -0.2376159522417609 2119 38-39 -1.6038585883814847 2119 40-41 -1.0769085272406471 2119 42-43 0.15015754173830942 2119 44-45 0.060093612310836875 2119 46-47 -0.11190155735371832 2119 48-49 -0.5490701879090096 2119 50-51 0.8916352087845141 2119 52-53 0.2481796236718381 2119 54-55 0.24882144338506862 2119 56-57 -1.4123931002926895 2119 58-59 -0.1095400231044934 2119 60-61 0.38779930299770626 2119 62-63 -0.009155030183961799 2119 64-65 1.9315593283408816E-4 2119 66-67 0.5885919923132619 2119 68-69 0.5553603725684368 2119 70-71 -0.13282350650873553 2119 72-73 0.7328481633769357 2119 74-75 0.31796734030497475 2119 76 0.25276122567859005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 4.0 21 11.0 22 9.0 23 17.0 24 62.0 25 134.0 26 256.0 27 508.0 28 846.0 29 1471.0 30 2262.0 31 3534.0 32 5488.0 33 8584.0 34 12909.0 35 18605.0 36 29156.0 37 55880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.106399879774145 22.162347839154982 11.37207754567509 30.359174735395776 2 17.686081710642135 19.310561984299078 40.3321954815117 22.671160823547094 3 16.674180782469925 26.061816126008143 33.73694869647982 23.527054395042114 4 11.779986689280578 21.424533230282602 37.81460887238169 28.98087120805513 5 10.742323078354337 38.75136864252131 37.49758474849181 13.008723530632546 6 23.59146110192719 39.90711121607019 18.129056727996165 18.372370954006456 7 23.503438602517587 33.69043274150726 22.046415766761847 20.759712889213308 8 20.790484982502843 36.492840121084605 22.365586780881227 20.35108811553132 9 21.155456321518283 18.210638556717264 20.562198988099073 40.07170613366539 10-11 21.51398698984521 25.69899167722221 29.538704852687548 23.24831648024503 12-13 23.293758990102837 21.579824956883286 27.285543556824603 27.840872496189267 14-15 22.674023343853094 24.175773059390142 22.165568174499235 30.98463542225753 16-17 23.7234948510416 27.551757945282922 23.0529494693603 25.671797734315177 18-19 22.009560817822052 27.094470326398877 24.127110214188082 26.768858641590988 20-21 21.238827225430633 25.777353170599053 23.970745042472643 29.01307456149767 22-23 22.83969170656304 28.722170935400072 24.16038701274537 24.277750345291512 24-25 22.955623778956184 29.877913508948957 24.213701453444685 22.952761258650177 26-27 21.62419402162634 32.18260017031996 24.471686096023245 21.721519712030457 28-29 19.51487437114007 29.097876725563022 29.04778262020796 22.339466283088946 30-31 21.117170112425484 27.210402398792016 29.196275861081887 22.47615162770061 32-33 20.22477940702892 24.97334277965034 31.593994432398002 23.207883380922734 34-35 22.148393052663216 26.124433757701972 31.41580254334929 20.311370646285525 36-37 20.361464751640582 26.773152422049996 30.40962665578909 22.455756170520335 38-39 20.881727817256703 25.456393081288418 28.582265255444156 25.079613846010716 40-41 20.26700158154247 25.5640954078018 29.921924758653756 24.246978252001973 42-43 23.20430523054023 25.163342564961322 30.32768701202974 21.304665192468708 44-45 20.27523132742223 26.208878106729074 28.98981658401139 24.52607398183731 46-47 23.279804203611068 23.69701653821107 27.984356326527692 25.038822931650174 48-49 21.977357464379512 23.5120261634356 28.97013675690762 25.540479615277274 50-51 19.03933818530525 23.678052341183793 31.18608528879252 26.096524184718433 52-53 19.90560839290954 23.624737900484483 33.79920851313539 22.67044519347059 54-55 18.68545911247558 23.968955967281396 31.701338943873132 25.644245976369895 56-57 21.935493104904214 23.77287332632016 29.42348841037091 24.868145158404715 58-59 19.249375612758254 27.434394612736785 30.959946184618246 22.356283589886715 60-61 20.189713533280376 31.936781239041917 25.873963230926673 21.99954199675104 62-63 17.294989873834417 35.18109019085854 24.87852179451398 22.64539814079306 64-65 17.661392473002856 36.281013618440355 23.794700043653435 22.262893864903354 66-67 17.600921731538534 35.767906853589245 23.758918539828393 22.872252875043834 68-69 17.392673379276786 34.70090240952647 24.131046179608838 23.775378031587913 70-71 18.156250670903194 33.66860602417398 24.04481275539048 24.130330549532335 72-73 18.097211189591878 31.810472530539514 24.28454883101827 25.80776744885034 74-75 18.15088344532944 31.39683834632202 24.48456743740026 25.967710770948283 76 17.813463864259287 31.69382482806987 24.979067820262348 25.513643487408487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 3.0 15 5.5 16 5.0 17 4.0 18 8.5 19 20.5 20 29.5 21 31.0 22 44.0 23 83.5 24 113.5 25 117.0 26 210.0 27 395.5 28 587.0 29 686.0 30 854.0 31 1190.0 32 1657.0 33 1956.0 34 2189.0 35 2820.5 36 3731.0 37 4243.0 38 5953.5 39 9252.0 40 11308.0 41 11308.0 42 10840.0 43 10487.5 44 10308.0 45 10162.5 46 9844.0 47 9726.0 48 9081.5 49 7970.0 50 7385.0 51 6857.0 52 5575.0 53 4515.5 54 4210.0 55 3916.0 56 3065.5 57 2214.0 58 1919.0 59 1530.0 60 949.0 61 591.5 62 426.0 63 371.5 64 268.5 65 173.0 66 96.0 67 66.0 68 55.5 69 33.0 70 13.5 71 6.0 72 4.5 73 1.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 139737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.16775800253333 #Duplication Level Percentage of deduplicated Percentage of total 1 60.4733479945544 20.662387198809192 2 15.122002303906168 10.33369830467235 3 7.569378992564666 7.758861289422272 4 4.082102837993507 5.579052076400667 5 2.607602890355011 4.454797226217823 6 1.9583202429573776 4.014684729169797 7 1.4095716828987328 3.3713332903955284 8 1.024191014765944 2.799544859271345 9 0.8168394596292806 2.5118615685180017 >10 4.829825112577233 27.787200240451703 >50 0.07540056550424129 1.7325404152085704 >100 0.023039061681851502 1.5479078554713497 >500 0.0020944601528955914 0.5009410535505987 >1k 0.006283380458686774 6.9451898924407995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT 4223 3.0221058130631118 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT 3967 2.8389045134788926 No Hit CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 1515 1.0841795658987956 No Hit CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 700 0.5009410535505987 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 386 0.2762332095293301 No Hit TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT 353 0.25261741700480184 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 259 0.1853481898137215 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 202 0.14455727545317273 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 179 0.12809778369365307 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT 145 0.10376636109262401 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005725040612006842 0.0 2 0.0 0.0 0.0 0.005725040612006842 0.0 3 0.0 0.0 0.0 0.006440670688507697 0.0 4 0.0 0.0 0.0 0.007871930841509407 0.0 5 0.0 0.0 0.0 0.007871930841509407 0.0 6 0.0 0.0 0.0 0.007871930841509407 0.0 7 0.0 0.0 0.0 0.007871930841509407 0.0 8 0.0 0.0 0.0 0.007871930841509407 0.0 9 0.0 0.0 0.0 0.012881341377015394 0.0 10 0.0 0.0 0.0 0.015028231606517959 0.0 11 0.0 0.0 0.0 0.015743861683018813 0.0 12 0.0 0.0 0.0 0.01645949175951967 0.0 13 0.0 0.0 0.0 0.020037642142023946 0.0 14 0.0 0.0 0.0 0.022900162448027367 0.0 15 0.0 0.0 0.0 0.02504705267752993 0.0 16 0.0 0.0 0.0 0.030772093289536773 0.0 17 0.0 0.0 0.0 0.03435024367204105 0.0 18 0.0 0.0 0.0 0.04436906474305302 0.0 19 0.0 0.0 0.0 0.05367225573756414 0.0 20 0.0 0.0 0.0 0.05725040612006842 0.0 21 0.0 0.0 0.0 0.06154418657907355 0.0 22 0.0 0.0 0.0 0.06297544673207525 0.0 23 0.0 0.0 0.0 0.06512233696157782 0.0 24 0.0 0.0 0.0 0.06583796703807868 0.0 25 0.0 0.0 0.0 0.06655359711457953 0.0 26 0.0 0.0 0.0 0.07013174749708381 0.0 27 0.0 0.0 0.0 0.08659123925660347 0.0 28 0.0 0.0 0.0 0.10734451147512827 0.0 29 0.0 0.0 0.0 0.12523526338764965 0.0 30 0.0 0.0 0.0 0.1517135762181813 0.0 31 0.0 0.0 0.0 0.18177003943121722 0.0 32 0.0 0.0 0.0 0.2089639823382497 0.0 33 0.0 0.0 0.0 0.2504705267752993 0.0 34 0.0 0.0 0.0 0.31845538404288054 0.0 35 0.0 0.0 0.0 0.40361536314648233 0.0 36 0.0 0.0 0.0 0.5252724761516278 0.0 37 0.0 0.0 0.0 0.7943493849159492 0.0 38 0.0 0.0 0.0 1.081317045592792 0.0 39 0.0 0.0 0.0 1.442710234225724 0.0 40 0.0 0.0 0.0 1.8942728124977637 0.0 41 0.0 0.0 0.0 2.3386790900047947 0.0 42 0.0 0.0 0.0 2.891145509063455 0.0 43 0.0 0.0 0.0 3.440749407816112 0.0 44 0.0 0.0 0.0 3.9509936523612215 0.0 45 0.0 0.0 0.0 4.498450660884376 0.0 46 0.0 0.0 0.0 5.033741958107015 0.0 47 0.0 0.0 0.0 5.5776208162476655 0.0 48 0.0 0.0 0.0 6.0807087600277665 0.0 49 0.0 0.0 0.0 6.61886257755641 0.0 50 0.0 0.0 0.0 7.143419423631537 0.0 51 0.0 0.0 0.0 7.6744169403951705 0.0 52 0.0 0.0 0.0 8.180367404481276 0.0 53 0.0 0.0 0.0 8.684886608414379 0.0 54 0.0 0.0 0.0 9.208727824413005 0.0 55 0.0 0.0 0.0 9.750459792324152 0.0 56 0.0 0.0 0.0 10.254263366180753 0.0 57 0.0 0.0 0.0 10.716560395600306 0.0 58 0.0 0.0 0.0 11.189591876167372 0.0 59 0.0 0.0 0.0 11.668348397346444 0.0 60 0.0 0.0 0.0 12.139948617760508 0.0 61 0.0 0.0 0.0 12.613695728404073 0.0 62 0.0 0.0 0.0 13.128233753408189 0.0 63 0.0 0.0 0.0 13.603412124204755 0.0 64 0.0 0.0 0.0 14.021340088881255 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGCTA 20 7.9043006E-5 70.0 26 GGCGAAG 20 7.9043006E-5 70.0 43 TAGGGCG 25 2.3837485E-4 55.999996 40 CCTGCGG 25 2.3837485E-4 55.999996 53 GTAATCA 20 0.006939325 52.5 4 TGCGATG 20 0.006939325 52.5 28 ACATGTA 20 0.006939325 52.5 34 TTTGAAC 20 0.006939325 52.5 31 CTTAGGA 20 0.006939325 52.5 27 CTATACA 155 0.0 51.935486 1 GGAGTAA 35 2.0779818E-5 50.0 1 TTTACAC 190 0.0 49.73684 2 AACCGCC 30 5.861588E-4 46.666664 19 TGTATTC 30 5.861588E-4 46.666664 21 GATCACA 30 5.861588E-4 46.666664 1 GCGGCGT 30 5.861588E-4 46.666664 56 CTTTATA 55 1.3015597E-7 44.545452 2 TTTATAC 75 7.4760464E-10 42.0 3 CGCCTTT 35 0.0012519866 40.0 22 GTTACTT 35 0.0012519866 40.0 46 >>END_MODULE