FastQCFastQC Report
Wed 25 May 2016
SRR1780423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831281
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGGTC84351.0146990006989214No Hit
CTTTACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGG15280.18381269390254318No Hit
CTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTGG13950.16781329057202077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT11600.037.510943
GGTGGTC12050.035.9255444
GGACACC454.0232102E-529.38099919
TCGGTGG16850.029.2180142
CTCGGTG16950.028.39729341
ACGAAGT17500.028.3299721
CAGTGTA17500.027.76434731
ATGCAGT17900.027.63853528
GCAGTGT17700.027.58147830
TATGCAG18200.027.30376627
CGGTTGA655.952643E-727.06377229
CGAAGTA18550.027.04846622
GACGAAG18750.026.5636320
TCTCGGT18250.026.28726240
CTATACA3600.026.2697581
CGACGAA19800.026.1535419
GTAGATC19300.025.99736235
ATCTCGG20000.025.8576839
AAGTATG19400.025.7638924
AGATCTC20000.025.63761337
Produced by FastQC (version 0.11.5)