FastQCFastQC Report
Wed 25 May 2016
SRR1780423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831281
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT316953.8127901395556973No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT258893.114350021232291RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC85191.0248038870129355No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC43590.5243714219379487No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG34020.4092478957175732No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT23810.2864254084960441RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC22250.26765919105573205No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC14170.17045980841616734No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG12330.14832529553785062No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG8490.10213152953092877No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC8420.1012894556714276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC15100.041.81131418
CTTGAAA15700.041.19424427
TCTTCTG15300.041.1209820
CTGCTTG16200.039.51543824
TTGAAAA16550.039.34436428
GTCTTCT16150.039.0929319
CTTCTGC16350.039.01836821
TTCTGCT17250.037.36520422
TCTGCTT17500.037.2085323
CTTATAA1450.036.4416431
GCTTGAA17900.035.39388726
TGCTTGA17950.035.29530325
TGAAAAA18450.035.2926429
CTATACA5800.034.923241
GAAAAAA19900.032.7210730
CTTTACA11950.032.0579761
ATGATTC1100.032.00135840
TTTACAC12000.031.8994332
CGTGCGA752.1627784E-929.33634210
ATCGTAT300.00574111529.3363429
Produced by FastQC (version 0.11.5)