FastQCFastQC Report
Wed 25 May 2016
SRR1780422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86782
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAAT150.002218827669.9930835
AGCCTTG150.002218827669.993088
TAAGGCA150.002218827669.9930847
CACAATT150.002218827669.9930820
AGGCAGC252.2995367E-456.384451
TCTTGAA200.00693320952.49481258
GGGTAGA200.00693320952.49481234
AGAACGC200.00693320952.4948125
GCATTGG200.00693320952.49481251
TTGAATA200.00693320952.49481260
AGTTAGC200.00693320952.4948127
TGAATAG200.00693320952.49481261
AAGGCAT200.00693320952.49481248
AAAGCGA200.00693320952.49481225
GACTAAG200.00693320952.49481244
ATAGAGT350.001249970439.99604864
CTAGTAA350.001249970439.9960484
TATAAGA459.137055E-538.8850482
GTATAAG501.630494E-435.240281
TCCATCG501.6984927E-434.996548
Produced by FastQC (version 0.11.5)