##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86782 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.635143232467563 34.0 23.0 34.0 12.0 34.0 2 29.37551565992948 34.0 27.0 34.0 12.0 34.0 3 29.685868037150563 34.0 31.0 34.0 11.0 34.0 4 29.83119771381162 34.0 31.0 34.0 11.0 34.0 5 29.678147542117028 34.0 31.0 34.0 11.0 34.0 6 32.31627526445576 38.0 31.0 38.0 11.0 38.0 7 32.10632389205135 37.0 31.0 38.0 11.0 38.0 8 31.49945841303496 37.0 27.0 38.0 11.0 38.0 9 31.863969486759927 37.0 28.0 38.0 12.0 38.0 10-11 31.99542531861446 37.0 29.5 38.0 11.0 38.0 12-13 32.26658177963172 37.0 31.0 38.0 11.0 38.0 14-15 31.92853356686871 37.0 31.0 38.0 11.0 38.0 16-17 31.749435366781128 37.0 27.5 38.0 11.0 38.0 18-19 31.749590928994493 37.0 29.0 38.0 11.0 38.0 20-21 31.852480929224953 37.0 29.0 38.0 11.0 38.0 22-23 31.723249060865157 37.0 27.5 38.0 11.0 38.0 24-25 32.07623700767441 37.0 31.0 38.0 11.0 38.0 26-27 32.030161784701896 37.0 31.0 38.0 11.0 38.0 28-29 32.07265331520361 37.0 31.0 38.0 11.0 38.0 30-31 31.9284932359245 37.0 31.0 38.0 11.0 38.0 32-33 31.757109769307 37.0 29.0 38.0 11.0 38.0 34-35 31.724856537069897 37.0 27.0 38.0 11.0 38.0 36-37 31.695674218155837 37.0 27.0 38.0 11.0 38.0 38-39 32.03050171694591 37.0 31.0 38.0 11.0 38.0 40-41 31.677438869811713 37.0 27.0 38.0 11.0 38.0 42-43 31.980572008020097 37.0 30.0 38.0 11.0 38.0 44-45 31.230393399552902 37.0 26.5 38.0 11.0 38.0 46-47 31.831612546380583 37.0 28.0 38.0 11.0 38.0 48-49 31.675825632043512 37.0 27.0 38.0 11.0 38.0 50-51 31.61635477403148 37.0 28.0 38.0 11.0 38.0 52-53 31.629312530248207 37.0 27.0 38.0 11.0 38.0 54-55 31.432555138162293 37.0 27.0 38.0 11.0 38.0 56-57 31.415045746813853 37.0 27.0 38.0 11.0 38.0 58-59 31.31697241363416 37.0 26.5 38.0 11.0 38.0 60-61 31.349726901892097 37.0 27.0 38.0 11.0 38.0 62-63 31.370727800695995 37.0 27.0 38.0 11.0 38.0 64-65 31.446578783618726 37.0 27.0 38.0 11.0 38.0 66-67 31.472119794427414 37.0 27.0 38.0 11.0 38.0 68-69 31.092818787306122 37.0 26.0 38.0 11.0 38.0 70-71 31.035652554677235 37.0 26.0 38.0 11.0 38.0 72-73 31.09543453711599 37.0 26.5 38.0 11.0 38.0 74-75 30.7097209098661 36.5 25.0 38.0 11.0 38.0 76 29.90205342121638 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.5724221164824783 1101 2 -0.8368608176765697 1101 3 -0.6682455457384648 1101 4 -1.7882763862977065 1101 5 -1.0619241658383878 1101 6 -2.9075061439281136 1101 7 -1.7563573338356697 1101 8 -2.1280304422981224 1101 9 -1.1357876981117059 1101 10-11 -2.0734069463896923 1101 12-13 -1.0613068873573681 1101 14-15 -0.964665058197383 1101 16-17 -1.832491022445744 1101 18-19 -0.6349909190945908 1101 20-21 -1.2616848998180679 1101 22-23 -0.5552710689498923 1101 24-25 -1.4921472438283274 1101 26-27 -1.5495546130228206 1101 28-29 -0.6853021938951507 1101 30-31 -1.5635742350751904 1101 32-33 -0.9510904846067092 1101 34-35 -1.095491263469416 1101 36-37 -1.3891935352302056 1101 38-39 -1.213333439719893 1101 40-41 -0.3271957869352491 1101 42-43 -1.2932409284520716 1101 44-45 -0.8951038239236517 1101 46-47 -0.2821647596575225 1101 48-49 -1.377761439636405 1101 50-51 -1.7425669790212588 1101 52-53 -0.949184685705589 1101 54-55 -1.9515297595240746 1101 56-57 -1.479743695345718 1101 58-59 -1.2635498669168257 1101 60-61 -1.4931722091719948 1101 62-63 -2.4008690772747308 1101 64-65 -1.5144086686333544 1101 66-67 -2.109797039132694 1101 68-69 -0.6224951606757152 1101 70-71 -0.8184499592342966 1101 72-73 -1.0415786174985016 1101 74-75 -1.5502162873589356 1101 76 -3.134187149546346 1102 1 0.31064323729208354 1102 2 0.11195300923771967 1102 3 -0.09464464831854968 1102 4 -0.04119507839127223 1102 5 0.5179088143435919 1102 6 0.7252164452945351 1102 7 1.2276469870750475 1102 8 1.6256776469453023 1102 9 0.8428570316236339 1102 10-11 -0.234527059398129 1102 12-13 -3.233613588923699 1102 14-15 -0.819187472921719 1102 16-17 -2.392059346846427 1102 18-19 -0.6526900341388391 1102 20-21 -0.14359731828165323 1102 22-23 -0.7081752195170097 1102 24-25 0.15780705423141939 1102 26-27 -4.175421911207202 1102 28-29 0.006614794762455745 1102 30-31 0.02268817715631144 1102 32-33 -5.606487468278651 1102 34-35 -0.3690485363099967 1102 36-37 0.5343284224747222 1102 38-39 0.46048973531854287 1102 40-41 0.3285231876794015 1102 42-43 0.3332911252269284 1102 44-45 -5.67050994798365 1102 46-47 0.26032765797511814 1102 48-49 1.1204416431671973 1102 50-51 -4.6621315641722845 1102 52-53 -1.2466212223294626 1102 54-55 0.6569292541449592 1102 56-57 -0.8780464914735155 1102 58-59 0.8854592319240062 1102 60-61 0.6125446880817336 1102 62-63 -3.291620666455838 1102 64-65 -0.2716898186603629 1102 66-67 -0.46829760002014353 1102 68-69 -6.023363497540565 1102 70-71 -0.6258474414728603 1102 72-73 0.5473532035160815 1102 74-75 -5.752852458367286 1102 76 -2.3380385766933003 1103 1 -0.8603466949926784 1103 2 -0.6457410898629945 1103 3 0.056621877423989275 1103 4 -0.20136490390809314 1103 5 -0.45948361158167117 1103 6 -1.2884340867537567 1103 7 0.0020011843449410094 1103 8 0.3028300241442565 1103 9 0.36745306902824737 1103 10-11 -0.4553898016861133 1103 12-13 -1.226833108109684 1103 14-15 -0.17850817085443538 1103 16-17 -1.030006751095648 1103 18-19 -0.5823593401472209 1103 20-21 -0.6176845956991599 1103 22-23 -0.43612298204791244 1103 24-25 0.11879913620626326 1103 26-27 -0.4866970357100868 1103 28-29 -0.5128667716450508 1103 30-31 -0.2906560240546803 1103 32-33 -4.717384605608018 1103 34-35 -0.4098836909085435 1103 36-37 0.032277259850665985 1103 38-39 -0.0841969473513764 1103 40-41 0.11676080009028311 1103 42-43 -0.27369178473688294 1103 44-45 -4.767953608189302 1103 46-47 -1.0090374668860491 1103 48-49 -0.1377450932449662 1103 50-51 -0.5162930058977686 1103 52-53 -0.263729172599966 1103 54-55 0.2862151987546149 1103 56-57 -0.07007651547838734 1103 58-59 -0.035432172898463676 1103 60-61 0.7353636483482973 1103 62-63 0.29594337639465707 1103 64-65 0.1363392738121405 1103 66-67 -0.834094239337972 1103 68-69 -2.5367811464961747 1103 70-71 0.10881810257787095 1103 72-73 0.020661218277282956 1103 74-75 -1.3867333655365002 1103 76 0.12355362709732276 1104 1 -1.5570931951634002 1104 2 0.0267626904404068 1104 3 -0.13014949958200717 1104 4 0.9016382016314743 1104 5 0.16591677539132021 1104 6 0.9203687358727777 1104 7 0.7394286269087189 1104 8 1.3131709831218679 1104 9 0.4055325123074347 1104 10-11 0.08078729536606133 1104 12-13 -0.4679758165954482 1104 14-15 0.1203227804812741 1104 16-17 0.096718774488469 1104 18-19 0.8007520527929586 1104 20-21 0.9385809523228836 1104 22-23 0.2788258822351324 1104 24-25 0.7420288973561284 1104 26-27 0.8515710162548586 1104 28-29 0.17735972137155187 1104 30-31 0.01932072504698823 1104 32-33 -0.1736143830172523 1104 34-35 0.42345777210951496 1104 36-37 0.7353893031656327 1104 38-39 -0.01261507329645184 1104 40-41 1.3785285187381504 1104 42-43 0.8377336741838164 1104 44-45 -2.420190084458749 1104 46-47 0.47446740279510635 1104 48-49 0.7615281129400984 1104 50-51 0.5833281507937791 1104 52-53 1.047280046462518 1104 54-55 -0.30234145701827586 1104 56-57 0.8073766168891439 1104 58-59 0.34375258231672845 1104 60-61 -0.09060419061890812 1104 62-63 0.36337098155212644 1104 64-65 0.21999372969234088 1104 66-67 -0.026930361153169713 1104 68-69 -3.7554777954401253 1104 70-71 -0.5346952219754009 1104 72-73 0.6875067380080324 1104 74-75 -0.6973117113082523 1104 76 -0.4951804183963944 1105 1 0.3299367058170404 1105 2 0.09919818139206882 1105 3 -0.5506655719687217 1105 4 0.30470797058583443 1105 5 0.07897792687986538 1105 6 -0.17507376286712173 1105 7 0.7064546256404824 1105 8 0.20971261523737184 1105 9 0.8087486155869463 1105 10-11 1.0931931942653001 1105 12-13 -0.46772379636879435 1105 14-15 -0.24530979028763156 1105 16-17 1.0286977023456458 1105 18-19 0.6828228460875891 1105 20-21 -0.31404087100280265 1105 22-23 0.37966649163629995 1105 24-25 0.3637384074613266 1105 26-27 0.5882017497150116 1105 28-29 -0.7294659619284545 1105 30-31 0.27209701238256656 1105 32-33 1.0518461636227485 1105 34-35 0.106310641075261 1105 36-37 0.24482786713812033 1105 38-39 0.17491236438951674 1105 40-41 0.7604854964465559 1105 42-43 0.5259033569635179 1105 44-45 1.3474310071953148 1105 46-47 0.3188977557860184 1105 48-49 0.5140767691032124 1105 50-51 0.2205490993653889 1105 52-53 -0.8345793750291151 1105 54-55 -0.11257022634660174 1105 56-57 0.07972105537181307 1105 58-59 0.28440588378169807 1105 60-61 0.46208429612157786 1105 62-63 0.6744858865161127 1105 64-65 0.24767530620080436 1105 66-67 0.6780515096498831 1105 68-69 1.4450002300220461 1105 70-71 1.378048871808641 1105 72-73 1.2068960360910523 1105 74-75 -0.44138923598184476 1105 76 0.04258196717829321 1106 1 0.1199392589774888 1106 2 0.14854712547785454 1106 3 -0.5387569021653142 1106 4 -0.2693701561238697 1106 5 -0.44848678479537085 1106 6 -0.37806825533529675 1106 7 0.7531592249766632 1106 8 1.1893785159559052 1106 9 0.7254608744044724 1106 10-11 0.06217229482335185 1106 12-13 -0.44279400828111193 1106 14-15 -0.18576644127059794 1106 16-17 0.13921307601891897 1106 18-19 -0.31742335152702594 1106 20-21 -0.17793918226953664 1106 22-23 -0.11192631574598266 1106 24-25 0.030724219183980495 1106 26-27 -0.4061083498582434 1106 28-29 -0.6269656883755488 1106 30-31 -0.24810031044633618 1106 32-33 0.06984594478042538 1106 34-35 0.15193926565125437 1106 36-37 0.04945638229294502 1106 38-39 -0.3141455194052085 1106 40-41 -0.2864014714930434 1106 42-43 0.1618409139537107 1106 44-45 0.5736501413091837 1106 46-47 -0.11448299338420753 1106 48-49 0.25645376148020205 1106 50-51 -0.05039593473754067 1106 52-53 -0.133171489410703 1106 54-55 -0.40800736388900205 1106 56-57 -0.07716153572130224 1106 58-59 -0.07693014860696579 1106 60-61 -0.05258725802366371 1106 62-63 0.09158111940081781 1106 64-65 0.31483254441067743 1106 66-67 0.04519598205750697 1106 68-69 0.5643148888103902 1106 70-71 -0.12796017367409007 1106 72-73 0.37851401034060217 1106 74-75 -1.080764441146524 1106 76 -0.6565827846180383 1107 1 0.2894508758575256 1107 2 0.1801698413110948 1107 3 0.4007514320798613 1107 4 0.2115905616789533 1107 5 0.29355282566529084 1107 6 0.39577643956203445 1107 7 0.657871629689069 1107 8 0.9425061375045765 1107 9 -0.30461170829968864 1107 10-11 0.1276061497308909 1107 12-13 0.29543409836805523 1107 14-15 -0.1683867133645549 1107 16-17 0.4335560019407865 1107 18-19 0.9945637367758522 1107 20-21 0.6616676310214906 1107 22-23 -0.4928031439912317 1107 24-25 0.0034145208216500578 1107 26-27 0.07403170922918179 1107 28-29 -0.1849315489729868 1107 30-31 0.18707676946758767 1107 32-33 0.8332226818413702 1107 34-35 0.5111689406704016 1107 36-37 -0.14383610047321582 1107 38-39 0.790296979774137 1107 40-41 0.47506039523197785 1107 42-43 0.7323810897570482 1107 44-45 0.7199006428228429 1107 46-47 0.05573986104916884 1107 48-49 0.33391482578337417 1107 50-51 0.6841118524018306 1107 52-53 0.8455825682907232 1107 54-55 0.6991706643416613 1107 56-57 0.36109757359043826 1107 58-59 0.7439200538221833 1107 60-61 0.18475636089890557 1107 62-63 0.05910127113149599 1107 64-65 0.7476753062008044 1107 66-67 1.4128693234150589 1107 68-69 1.0968220923702248 1107 70-71 0.5845266046021642 1107 72-73 0.47612680532181884 1107 74-75 0.469541938107227 1107 76 -0.30559617047352816 1108 1 -0.10731025774571279 1108 2 0.6862427158050224 1108 3 -0.08102994443835598 1108 4 0.44640837544413614 1108 5 0.4635933114952522 1108 6 0.9666266419911835 1108 7 1.313742075033197 1108 8 1.829145813617938 1108 9 0.30672432408897166 1108 10-11 0.5931931942653001 1108 12-13 1.2225596044409244 1108 14-15 1.3963906145706702 1108 16-17 0.5651349493092042 1108 18-19 1.2395030080309155 1108 20-21 1.698104877985049 1108 22-23 0.5254154794905475 1108 24-25 0.9831716058418962 1108 26-27 0.8918454744113689 1108 28-29 1.1794409206626355 1108 30-31 1.2417326399129323 1108 32-33 1.6105506170640425 1108 34-35 0.7095495074720191 1108 36-37 0.9492813084336618 1108 38-39 1.7275439433368867 1108 40-41 1.7382182899688203 1108 42-43 1.4489802800404519 1108 44-45 1.2300221003127199 1108 46-47 2.0658613185390564 1108 48-49 1.2914047043258847 1108 50-51 1.3905895851953467 1108 52-53 1.0500360095862646 1108 54-55 1.2214378708193898 1108 56-57 1.7841744966673616 1108 58-59 0.25404151131206376 1108 60-61 1.2677523123159062 1108 62-63 1.3951336597954658 1108 64-65 1.8934242940550519 1108 66-67 1.892626408435305 1108 68-69 2.0279961814390504 1108 70-71 2.481287738205406 1108 72-73 1.9477867243501592 1108 74-75 1.8035500352732186 1108 76 1.884687230336187 1109 1 -0.6657698372154783 1109 2 -1.3786978614445538 1109 3 -0.9343177856294247 1109 4 -1.1436072964900426 1109 5 -1.3955225302182122 1109 6 -0.8247407342658377 1109 7 -1.1681209261421976 1109 8 0.4047955458890655 1109 9 -0.11343043626103366 1109 10-11 -0.396905734819196 1109 12-13 -1.2087777339422558 1109 14-15 -0.5708289231072712 1109 16-17 -1.4663884932923317 1109 18-19 -0.8265231771591068 1109 20-21 -0.5904464475993798 1109 22-23 -0.7032151852606603 1109 24-25 -0.4382891490229355 1109 26-27 -0.05982456607446096 1109 28-29 -0.9042651652717986 1109 30-31 -0.7267765673483986 1109 32-33 -0.6933951188638616 1109 34-35 -0.7475812225789085 1109 36-37 -0.6984185087271015 1109 38-39 -0.8174149178359009 1109 40-41 -0.3193679056691572 1109 42-43 -0.7641253749157997 1109 44-45 -0.5318787747036033 1109 46-47 -0.9417134496469224 1109 48-49 -0.6641180199786731 1109 50-51 0.12242809897358953 1109 52-53 0.11820892326003474 1109 54-55 -0.14433038045395463 1109 56-57 0.015580399762569641 1109 58-59 -0.5265269228005138 1109 60-61 -0.6621484321009632 1109 62-63 -0.5915778460162109 1109 64-65 -1.0877938723286462 1109 66-67 -0.7716268892008458 1109 68-69 0.15080864062672106 1109 70-71 -0.6557682236595603 1109 72-73 -1.1143296397611095 1109 74-75 -0.1384148334743358 1109 76 -1.1548027788057738 1110 1 0.8396119929359358 1110 2 0.18097955791028753 1110 3 -0.3698839475780744 1110 4 -0.024417039742182567 1110 5 0.40694619920904174 1110 6 0.7564969220870239 1110 7 1.08110553863726 1110 8 1.4433406413465981 1110 9 -1.0957378753657068 1110 10-11 -0.5023616268534283 1110 12-13 0.12640988949421939 1110 14-15 -0.02303432139562034 1110 16-17 -0.024107387301548044 1110 18-19 0.6139886727421455 1110 20-21 0.9965598065151688 1110 22-23 0.9025616415991209 1110 24-25 0.6425370524253893 1110 26-27 -0.3631408926050739 1110 28-29 0.48971936402379157 1110 30-31 0.45961250440933554 1110 32-33 0.7932325967385054 1110 34-35 0.4431692711669726 1110 36-37 0.22176746888975174 1110 38-39 0.4369300459560712 1110 40-41 0.21398462889305492 1110 42-43 0.5395941774212574 1110 44-45 0.9745234636110744 1110 46-47 0.8342575839278012 1110 48-49 0.8544221380200128 1110 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0.6590860854053453 2116 40-41 0.06210492964493497 2116 42-43 0.251887898737543 2116 44-45 1.4269304551158122 2116 46-47 0.7806202438242948 2116 48-49 0.864831277752458 2116 50-51 0.9511343036348059 2116 52-53 0.8803635767559399 2116 54-55 -0.1015286363576422 2116 56-57 -0.3343385692141254 2116 58-59 -0.5900880433438083 2116 60-61 -1.0174887623988482 2116 62-63 0.6835031850070941 2116 64-65 0.6326587438158242 2116 66-67 -0.05271772111935036 2116 68-69 0.10271094036433936 2116 70-71 -1.9535668808597428 2116 72-73 0.289339908008607 2116 74-75 -1.1673737777558593 2116 76 -0.6169690081621546 2117 1 0.43124371411756 2117 2 0.740796909508461 2117 3 0.6460511936016857 2117 4 0.33713360791414004 2117 5 -0.2409710498823756 2117 6 -0.8678251355031357 2117 7 -1.014766234729617 2117 8 -1.9804968388509572 2117 9 0.03528439655676152 2117 10-11 -0.1522544606992966 2117 12-13 0.48605950276418497 2117 14-15 -0.4854702546730394 2117 16-17 -0.14537287979991476 2117 18-19 -0.10302744877495584 2117 20-21 0.05290124720103151 2117 22-23 0.46428002758364784 2117 24-25 0.38784503863356434 2117 26-27 0.891374064062525 2117 28-29 -0.1527277518088468 2117 30-31 -0.334395509755236 2117 32-33 1.0539259151617628 2117 34-35 0.04660790689523964 2117 36-37 -0.6094970917290183 2117 38-39 -0.04325819372336426 2117 40-41 -0.3833408766516264 2117 42-43 -0.6911764870088959 2117 44-45 0.39504345242852423 2117 46-47 -0.4451012829066059 2117 48-49 -0.8220665396428366 2117 50-51 0.5349428286834161 2117 52-53 0.9363706554739082 2117 54-55 -0.6968510020477083 2117 56-57 -0.09200171836970483 2117 58-59 -0.11460138148367705 2117 60-61 0.216294637570563 2117 62-63 -0.1266713796735992 2117 64-65 0.005656930440537167 2117 66-67 -1.232602364233987 2117 68-69 0.1818330919450304 2117 70-71 0.6398110221792983 2117 72-73 0.7027457763531899 2117 74-75 -0.49095202599164267 2117 76 -0.35175741129951277 2118 1 0.6546259280253892 2118 2 0.2065302187032394 2118 3 0.4137175987673345 2118 4 0.5674556564728661 2118 5 0.10771038153395196 2118 6 0.7824071980916969 2118 7 1.0496032460902853 2118 8 -3.154213975213679 2118 9 0.23169177490850146 2118 10-11 0.13676450818207897 2118 12-13 0.6121331655887943 2118 14-15 -0.6612793991624102 2118 16-17 -0.03902242820777957 2118 18-19 -0.36626845213423564 2118 20-21 -1.7613022810511332 2118 22-23 -0.23225810127600255 2118 24-25 0.04019955354917926 2118 26-27 0.5693053898726745 2118 28-29 -0.09728953645269911 2118 30-31 0.7496871245657744 2118 32-33 0.41703013337430406 2118 34-35 1.4270315385408878 2118 36-37 0.9981407494795178 2118 38-39 0.6019754473765495 2118 40-41 -0.5079159375009041 2118 42-43 0.24415230022985668 2118 44-45 0.5186932019910131 2118 46-47 0.013309997844906718 2118 48-49 -0.057085548755139826 2118 50-51 0.3292722797324359 2118 52-53 -0.48140739051003933 2118 54-55 0.3324974581136182 2118 56-57 1.1706358211068917 2118 58-59 0.6576513491903917 2118 60-61 -0.7000552105576929 2118 62-63 0.2391383682796544 2118 64-65 -0.14912149773719818 2118 66-67 -1.1813984537880273 2118 68-69 1.3753539013912572 2118 70-71 0.21607879921950612 2118 72-73 -1.1464988880203109 2118 74-75 0.8504476079069363 2118 76 -0.6533284289407746 2119 1 0.6594199161052039 2119 2 0.4584305383024443 2119 3 0.5486834793927144 2119 4 0.7695805592974345 2119 5 0.7067861351834317 2119 6 1.1339879184855874 2119 7 -0.7176939811706617 2119 8 0.7863578482293541 2119 9 -0.4700876152616651 2119 10-11 0.7104234871921911 2119 12-13 1.1724683128635647 2119 14-15 0.10192566259797786 2119 16-17 0.1811447135387887 2119 18-19 0.0610875122779575 2119 20-21 -1.007231710091478 2119 22-23 0.849520425112523 2119 24-25 0.40698283073670893 2119 26-27 0.20610680647455837 2119 28-29 0.8517041579413629 2119 30-31 0.8406649253777232 2119 32-33 -0.13563895188094222 2119 34-35 0.6695598273894632 2119 36-37 -0.4572341317505071 2119 38-39 0.46990323523177935 2119 40-41 0.7362535364560827 2119 42-43 -0.3350456785211122 2119 44-45 -0.8660027468731144 2119 46-47 0.3294756711626903 2119 48-49 -0.16107406045938433 2119 50-51 0.8277174727876293 2119 52-53 -0.925434340497084 2119 54-55 -1.4817355037936117 2119 56-57 1.0993090525139948 2119 58-59 1.0946384788440149 2119 60-61 1.1793284812450224 2119 62-63 -0.14020213444125673 2119 64-65 0.9704366779559876 2119 66-67 0.7656517319250256 2119 68-69 0.1472507658639124 2119 70-71 -0.03310060822628813 2119 72-73 -1.0330519674018461 2119 74-75 0.6791057231354074 2119 76 1.8558708454826522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 70.0 13 439.0 14 928.0 15 1529.0 16 1937.0 17 2339.0 18 2493.0 19 2500.0 20 2190.0 21 1753.0 22 1316.0 23 1106.0 24 969.0 25 935.0 26 987.0 27 1039.0 28 1192.0 29 1461.0 30 1867.0 31 2267.0 32 3043.0 33 3973.0 34 5524.0 35 8283.0 36 14814.0 37 21824.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.20176161353587 27.693771686530273 10.003365401353124 28.101101298580726 2 17.985296490055543 24.059136687331474 34.18220368279136 23.773363139821623 3 15.852365697955797 29.733124380631924 28.09684035859971 26.31766956281256 4 11.891866977022884 21.400751307874906 37.49395035836925 29.21343135673296 5 13.936069691871586 35.22619898135558 33.18314857919845 17.65458274757438 6 25.647023576317668 36.899357009518106 18.570671337374108 18.882948076790118 7 23.055472332972275 35.08446452029222 20.68862206448342 21.17144108225208 8 20.571086169943076 38.294807679011775 18.674379479615588 22.459726671429557 9 21.05505750040331 22.992095134935816 19.76216266045954 36.19068470420133 10-11 21.269963817381484 28.361296121315483 25.803738102371458 24.565001958931575 12-13 21.35638726924938 25.63838123113088 25.300753612500287 27.70447788711945 14-15 20.080777119679198 26.99868636353161 22.595123412689265 30.32541310409993 16-17 20.949044732778688 28.753082436449954 24.316678573897814 25.981194256873547 18-19 19.992049042428153 27.653776128690282 25.354912309004174 26.999262519877394 20-21 19.683805397432646 28.32672674056832 25.058191790924383 26.931276071074645 22-23 21.782742965131018 27.95625821022793 24.643935378304256 25.617063446336797 24-25 20.453550275402733 29.1051139637252 24.584015118342514 25.857320642529558 26-27 19.457952109884538 29.894448157452008 25.411375630891197 25.236224101772258 28-29 20.407457767739853 28.10433039109493 27.170957110921616 24.317254730243597 30-31 20.45239796271116 27.903827982761403 27.056301998110204 24.58747205641723 32-33 19.36576709455878 29.249729206517483 26.17766357078657 25.20684012813717 34-35 20.2737894955175 28.263349542531863 26.47035099444585 24.99250996750478 36-37 20.23979627111613 27.64513378350349 26.65184024336844 25.463229702011937 38-39 19.875089304233597 27.219354243967643 27.84045078472494 25.065105667073816 40-41 19.601415039985252 26.812011707496946 27.406028900002305 26.1805443525155 42-43 18.203659745108432 26.649535617985297 28.306561268465813 26.840243368440458 44-45 18.36210274019958 26.73192597543269 28.51973911640663 26.386232167961097 46-47 18.377658961535804 26.983706298541172 28.311746675577886 26.326888064345138 48-49 17.760595515199004 27.69295476020373 27.99601299808716 26.550436726510107 50-51 18.079786130764443 27.43829365536632 27.19112258302413 27.290797630845105 52-53 17.16024060289 27.570233458551314 27.530478670692077 27.739047267866606 54-55 17.07151252563896 27.78802055725842 27.128321541333456 28.012145375769173 56-57 17.717383789265053 27.43656518632896 27.137540042866032 27.708510981539952 58-59 17.29851812587864 27.17383789265055 26.916872162429996 28.610771819040814 60-61 17.202300016132376 26.47323177617478 27.10873222557673 29.215735982116108 62-63 17.357862229494593 27.158281671314327 26.80279320596437 28.681062893226706 64-65 17.934594731626376 27.4555783457399 25.296144361734 29.31368256089972 66-67 17.58486782973428 28.94782328132562 24.435942937475513 29.03136595146459 68-69 17.294485031458137 28.997372727063215 24.24408287432878 29.46405936714987 70-71 17.66495356179853 29.261828489778985 24.060865156368834 29.012352792053655 72-73 17.92191929201908 29.99124242354405 23.38964301352815 28.697195270908715 74-75 18.243414532967666 30.224009587241596 23.243875458044293 28.288700421746444 76 18.620220783111705 29.75156138369708 23.666198059505426 27.962019773685785 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 1.0 5 0.5 6 0.0 7 0.5 8 1.0 9 1.0 10 3.5 11 5.0 12 4.0 13 8.0 14 10.0 15 10.0 16 15.0 17 18.0 18 24.0 19 30.0 20 32.5 21 35.0 22 57.0 23 94.0 24 136.5 25 164.0 26 204.0 27 304.0 28 414.5 29 465.0 30 566.5 31 804.0 32 1136.5 33 1333.0 34 1505.5 35 1883.0 36 2320.5 37 2553.0 38 2933.0 39 3675.0 40 4429.0 41 5142.0 42 5463.0 43 5686.5 44 6045.5 45 6274.0 46 6367.0 47 6303.0 48 6173.5 49 5899.5 50 5691.0 51 5159.5 52 4164.0 53 3443.0 54 3186.0 55 2943.5 56 2361.5 57 1807.0 58 1592.0 59 1325.0 60 939.0 61 726.0 62 632.0 63 535.0 64 370.5 65 259.0 66 184.0 67 153.0 68 117.5 69 69.5 70 48.5 71 40.0 72 35.5 73 29.5 74 20.0 75 12.0 76 13.5 77 13.5 78 8.5 79 5.0 80 7.0 81 7.0 82 4.5 83 4.0 84 5.5 85 6.5 86 6.0 87 6.0 88 5.0 89 4.0 90 4.0 91 2.5 92 1.0 93 3.0 94 4.0 95 3.5 96 4.0 97 4.5 98 4.5 99 5.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7040630545504828 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 86782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.95722615288885 #Duplication Level Percentage of deduplicated Percentage of total 1 79.98392704275057 52.75517964554861 2 9.568650745121333 12.622433223479524 3 4.255839549957197 8.421101150008067 4 2.183825713237478 5.761563457859925 5 1.2543894896836074 4.136802562743426 6 0.7477419242125125 2.9591389919568574 7 0.5555652614476144 2.5650480514392386 8 0.34766505354740646 1.8344818049826 9 0.2882649941473471 1.7111843469843977 >10 0.8141302258949318 7.233066764997349 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00691387614943191 0.0 2 0.0 0.0 0.0 0.010370814224147864 0.0 3 0.0 0.0 0.0 0.01152312691571985 0.0 4 0.0 0.0 0.0 0.012675439607291835 0.0 5 0.0 0.0 0.0 0.01382775229886382 0.0 6 0.0 0.0 0.0 0.01382775229886382 0.0 7 0.0 0.0 0.0 0.01382775229886382 0.0 8 0.0 0.0 0.0 0.01382775229886382 0.0 9 0.0 0.0 0.0 0.01382775229886382 0.0 10 0.0 0.0 0.0 0.019589315756723746 0.0 11 0.0 0.0 0.0 0.021893941139867715 0.0 12 0.0 0.0 0.0 0.0230462538314397 0.0 13 0.0 0.0 0.0 0.0230462538314397 0.0 14 0.0 0.0 0.0 0.02765550459772764 0.0 15 0.0 0.0 0.0 0.031112442672443594 0.0 16 0.0 0.0 0.0 0.03687400613030352 0.0 17 0.0 0.0 0.0 0.03917863151344749 0.0 18 0.0 0.0 0.0 0.04378788227973543 0.0 19 0.0 0.0 0.0 0.0460925076628794 0.0 20 0.0 0.0 0.0 0.04954944573759536 0.0 21 0.0 0.0 0.0 0.05185407112073932 0.0 22 0.0 0.0 0.0 0.058767947270171234 0.0 23 0.0 0.0 0.0 0.06798644880274711 0.0 24 0.0 0.0 0.0 0.07144338687746307 0.0 25 0.0 0.0 0.0 0.07490032495217902 0.0 26 0.0 0.0 0.0 0.08181420110161093 0.0 27 0.0 0.0 0.0 0.11177433108248254 0.0 28 0.0 0.0 0.0 0.14288677375492614 0.0 29 0.0 0.0 0.0 0.17169459104422577 0.0 30 0.0 0.0 0.0 0.2223963494733931 0.0 31 0.0 0.0 0.0 0.2696411698278445 0.0 32 0.0 0.0 0.0 0.33186605517273166 0.0 33 0.0 0.0 0.0 0.4079186928164827 0.0 34 0.0 0.0 0.0 0.49549445737595355 0.0 35 0.0 0.0 0.0 0.608421101150008 0.0 36 0.0 0.0 0.0 0.769744877970086 0.0 37 0.0 0.0 0.0 1.0474522366389343 0.0 38 0.0 0.0 0.0 1.395450669493674 0.0 39 0.0 0.0 0.0 1.7849323592450048 0.0 40 0.0 0.0 0.0 2.2665990643220946 0.0 41 0.0 0.0 0.0 2.92572192390127 0.0 42 0.0 0.0 0.0 3.687400613030352 0.0 43 0.0 0.0 0.0 4.617316955128944 0.0 44 0.0 0.0 0.0 5.418174275771474 0.0 45 0.0 0.0 0.0 6.071535571892789 0.0 46 0.0 0.0 0.0 6.746790809153972 0.0 47 0.0 0.0 0.0 7.442787674863451 0.0 48 0.0 0.0 0.0 7.9901362033601435 0.0 49 0.0 0.0 0.0 8.570901799912424 0.0 50 0.0 0.0 0.0 9.13783964416584 0.0 51 0.0 0.0 0.0 9.796962503745016 0.0 52 0.0 0.0 0.0 10.57131663248139 0.0 53 0.0 0.0 0.0 11.527736166486138 0.0 54 0.0 0.0 0.0 12.408103062847134 0.0 55 0.0 0.0 0.0 13.129450807771196 0.0 56 0.0 0.0 0.0 13.838123113087967 0.0 57 0.0 0.0 0.0 14.430411836555967 0.0 58 0.0 0.0 0.0 14.973151114286372 0.0 59 0.0 0.0 0.0 15.519347330091493 0.0 60 0.0 0.0 0.0 16.054020418980894 0.0 61 0.0 0.0 0.0 16.644004517065753 0.0 62 0.0 0.0 0.0 17.193657670945587 0.0 63 0.0 0.0 0.0 17.814754211702887 0.0 64 0.0 0.0 0.0 18.4312415016939 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCAAT 15 0.0022188276 69.99308 35 AGCCTTG 15 0.0022188276 69.99308 8 TAAGGCA 15 0.0022188276 69.99308 47 CACAATT 15 0.0022188276 69.99308 20 AGGCAGC 25 2.2995367E-4 56.38445 1 TCTTGAA 20 0.006933209 52.494812 58 GGGTAGA 20 0.006933209 52.494812 34 AGAACGC 20 0.006933209 52.494812 5 GCATTGG 20 0.006933209 52.494812 51 TTGAATA 20 0.006933209 52.494812 60 AGTTAGC 20 0.006933209 52.494812 7 TGAATAG 20 0.006933209 52.494812 61 AAGGCAT 20 0.006933209 52.494812 48 AAAGCGA 20 0.006933209 52.494812 25 GACTAAG 20 0.006933209 52.494812 44 ATAGAGT 35 0.0012499704 39.996048 64 CTAGTAA 35 0.0012499704 39.996048 4 TATAAGA 45 9.137055E-5 38.885048 2 GTATAAG 50 1.630494E-4 35.24028 1 TCCATCG 50 1.6984927E-4 34.99654 8 >>END_MODULE