FastQCFastQC Report
Wed 25 May 2016
SRR1780422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86782
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT42524.89963356456408No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT36864.247424581134337Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC13601.5671452605378995No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC6090.7017584291673389No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG4520.5208453365905372No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT3380.3894816897513309No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC2150.24774722868797677No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1830.21087322255767324No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1800.20741628448295732No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT1340.15440990067064597No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1340.15440990067064597No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1240.14288677375492614No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT1080.12444977068977436No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGT960.11062201839091056No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT890.10255582954990666No Hit
CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC880.10140351685833468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG150.002217958270.062
TGCGGCT150.002217958270.043
TATTCAA150.002217958270.012
TGCCTTA150.002217958270.025
ACATGAG150.002217958270.09
CTATTCA150.002217958270.011
GAACATG150.002217958270.07
ATGCCTT150.002217958270.024
TCAAGAC150.002217958270.015
GTGCGGC150.002217958270.042
AAAGCCG150.002217958270.070
GTCGGGG150.002217958270.018
CTATACA1100.060.4545441
TTTACAC2400.056.8752
CGCGCCA252.3786926E-455.99999620
TCGTGTT252.3786926E-455.9999969
CGTGTTA252.3786926E-455.99999610
ATCGCGC252.3786926E-455.99999618
TCGCGCC252.3786926E-455.99999619
CTTTACA2500.054.6000021
Produced by FastQC (version 0.11.5)