##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780422_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86782 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.877566776520474 34.0 34.0 34.0 31.0 34.0 2 33.280311585351804 34.0 34.0 34.0 32.0 34.0 3 33.48257703210343 34.0 34.0 34.0 34.0 34.0 4 33.523115392592935 34.0 34.0 34.0 34.0 34.0 5 33.55591021179507 34.0 34.0 34.0 34.0 34.0 6 37.28576202438293 38.0 38.0 38.0 36.0 38.0 7 36.93724505081699 38.0 37.0 38.0 35.0 38.0 8 36.95151068193865 38.0 37.0 38.0 35.0 38.0 9 36.71882418012952 38.0 37.0 38.0 34.0 38.0 10-11 36.46619690719274 38.0 37.0 38.0 34.0 38.0 12-13 36.31806711069116 38.0 37.0 38.0 34.0 38.0 14-15 36.51871931967459 38.0 37.0 38.0 34.0 38.0 16-17 36.60572468945173 38.0 37.0 38.0 34.0 38.0 18-19 36.65806273190293 38.0 37.0 38.0 34.0 38.0 20-21 36.40580419902745 38.0 37.0 38.0 34.0 38.0 22-23 36.46185268834551 38.0 37.0 38.0 34.0 38.0 24-25 35.62219123781429 38.0 36.0 38.0 31.0 38.0 26-27 35.83262658154917 38.0 36.5 38.0 31.5 38.0 28-29 35.79261252333433 38.0 37.0 38.0 31.0 38.0 30-31 35.54767117605033 38.0 36.0 38.0 31.5 38.0 32-33 36.020545735290725 38.0 37.0 38.0 33.5 38.0 34-35 35.89907469290867 38.0 37.0 38.0 32.5 38.0 36-37 35.88599018229587 38.0 37.0 38.0 32.5 38.0 38-39 35.36611278836625 38.0 36.5 38.0 29.0 38.0 40-41 35.22307045239796 37.5 36.0 38.0 29.0 38.0 42-43 35.7793263580005 38.0 36.5 38.0 32.5 38.0 44-45 35.98228319236708 38.0 37.0 38.0 33.0 38.0 46-47 36.18872577262566 38.0 37.0 38.0 34.0 38.0 48-49 35.94134728399899 38.0 37.0 38.0 32.5 38.0 50-51 36.053778433315664 38.0 37.0 38.0 33.0 38.0 52-53 35.96147242515729 38.0 37.0 38.0 32.5 38.0 54-55 36.13783964416584 38.0 37.0 38.0 34.0 38.0 56-57 36.15094720103247 38.0 37.0 38.0 34.0 38.0 58-59 36.18778087621857 38.0 37.0 38.0 34.0 38.0 60-61 35.943986080062686 38.0 37.0 38.0 32.5 38.0 62-63 36.30209605678597 38.0 37.0 38.0 34.0 38.0 64-65 36.29737157475053 38.0 37.0 38.0 34.0 38.0 66-67 35.397086953515704 38.0 36.0 38.0 31.0 38.0 68-69 34.108824410592064 37.5 34.0 38.0 23.5 38.0 70-71 33.360685395588945 37.0 33.5 38.0 21.5 38.0 72-73 32.48103293309673 37.0 31.5 38.0 15.0 38.0 74-75 32.843890438109284 37.0 33.0 38.0 11.0 38.0 76 32.43560876679496 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36220251067125986 1101 2 0.2928377208928694 1101 3 0.2577394007865479 1101 4 0.28721893033843315 1101 5 0.34856296586180235 1101 6 -1.3033275304252427 1101 7 -0.22257150514460022 1101 8 0.26300196214963734 1101 9 0.46599293232880257 1101 10-11 0.11386289226366131 1101 12-13 0.4941635579006416 1101 14-15 0.6553930060819582 1101 16-17 -0.208999099857202 1101 18-19 -0.572473520711128 1101 20-21 -0.42762122320823437 1101 22-23 0.551917031685484 1101 24-25 -2.6091946045322985 1101 26-27 -0.6490158340507364 1101 28-29 0.22350310669083484 1101 30-31 1.2162311960080032 1101 32-33 0.018581014864928136 1101 34-35 0.12833220959798552 1101 36-37 0.15026875709254028 1101 38-39 0.16786233444996412 1101 40-41 1.0282679788874205 1101 42-43 0.7896805837567413 1101 44-45 0.12986278417347563 1101 46-47 0.6934459965730895 1101 48-49 -2.0086146062268284 1101 50-51 -1.7016066152932154 1101 52-53 -1.6222630362418116 1101 54-55 -1.1126408303654785 1101 56-57 -1.2532891020698713 1101 58-59 -1.4945725548358837 1101 60-61 -1.564038130670177 1101 62-63 -0.5569084265485031 1101 64-65 -0.44439790160271286 1101 66-67 -1.4041312879767602 1101 68-69 -1.822125973317874 1101 70-71 -1.6562099469747622 1101 72-73 -1.9314629276343211 1101 74-75 -0.5746507251415878 1101 76 -1.5424197529906003 1102 1 -0.4410714101397417 1102 2 0.23830699672485167 1102 3 0.12133988273428287 1102 4 0.31987504401162425 1102 5 0.08847737859186822 1102 6 0.3957875138225475 1102 7 0.37003724469959565 1102 8 0.654958420664741 1102 9 0.673625156351406 1102 10-11 0.5196961217289484 1102 12-13 0.7188093678636633 1102 14-15 -0.09985233024277562 1102 16-17 0.24343972186367324 1102 18-19 -0.3698269628981734 1102 20-21 0.5288892259171973 1102 22-23 0.5516989087888149 1102 24-25 -5.371876892953097 1102 26-27 -1.3429063155830008 1102 28-29 0.23547909239859877 1102 30-31 1.668836206602542 1102 32-33 0.651428245742828 1102 34-35 0.5290966784167921 1102 36-37 -2.2877843402525926 1102 38-39 0.04510069084200552 1102 40-41 0.8954830688371516 1102 42-43 0.9287598973966951 1102 44-45 0.9138795692497226 1102 46-47 1.0781109182565842 1102 48-49 -3.058793259266011 1102 50-51 -0.2153587445881584 1102 52-53 0.4778657601352734 1102 54-55 0.7634342039109825 1102 56-57 1.0900674977057747 1102 58-59 -0.17228039479599744 1102 60-61 -5.721491701653605 1102 62-63 -2.3179599866465566 1102 64-65 0.11707120804039306 1102 66-67 1.2404841925168242 1102 68-69 -0.5629959720714552 1102 70-71 -1.0454658362929763 1102 72-73 -5.039599950360646 1102 74-75 -2.015871797890764 1102 76 0.07150271989983992 1103 1 0.8388898954387045 1103 2 0.5877570343444276 1103 3 0.07676964161070998 1103 4 0.30027703605777134 1103 5 0.26837061065091206 1103 6 0.34444574892698654 1103 7 0.8044000407298384 1103 8 0.6883502733393172 1103 9 0.5452349159443983 1103 10-11 0.4444211405387364 1103 12-13 -0.7785930899486715 1103 14-15 -0.16858867075368522 1103 16-17 -0.06417197245038864 1103 18-19 0.6144265572193461 1103 20-21 -0.1897432129062011 1103 22-23 0.5521736320162134 1103 24-25 -3.0746105821795595 1103 26-27 -0.5602131121864886 1103 28-29 0.15639736834715023 1103 30-31 1.1392510967892093 1103 32-33 0.805811822110563 1103 34-35 0.21490155821328472 1103 36-37 -0.7222008785349914 1103 38-39 0.4547510079212884 1103 40-41 -0.16476199583269135 1103 42-43 -0.40786365604101604 1103 44-45 1.028923817037331 1103 46-47 0.8219932585525243 1103 48-49 -2.3404653598715015 1103 50-51 -0.45282784279316246 1103 52-53 0.48685863032982013 1103 54-55 -0.03018659075778629 1103 56-57 0.26036773775449973 1103 58-59 -0.11431937584289642 1103 60-61 -4.234506283718019 1103 62-63 -1.210574009791543 1103 64-65 0.3306966603710251 1103 66-67 -0.17142329915312615 1103 68-69 0.2394295949093035 1103 70-71 0.06458038204388572 1103 72-73 -3.9470110069333266 1103 74-75 -2.0608988481576844 1103 76 -2.9870225927009244 1104 1 -0.08278872186451736 1104 2 0.3238349492428867 1104 3 0.22877848670769652 1104 4 0.016502260843552108 1104 5 -0.4611547783544978 1104 6 0.19064837318921235 1104 7 0.4646279970896856 1104 8 0.4257939752725548 1104 9 0.7641998444844731 1104 10-11 -1.01284110683131 1104 12-13 -0.05844122743789626 1104 14-15 0.3162901156469715 1104 16-17 0.12620085489136557 1104 18-19 0.6205238207674597 1104 20-21 -0.4844117656597291 1104 22-23 0.15022575955470785 1104 24-25 0.7602267428289693 1104 26-27 0.9396486831730897 1104 28-29 1.1684943392266192 1104 30-31 0.7236128352409921 1104 32-33 0.17840340300905666 1104 34-35 -0.20975252370141106 1104 36-37 0.9334012872130231 1104 38-39 1.0868339957430209 1104 40-41 1.1579133208185226 1104 42-43 0.02278200352030524 1104 44-45 0.6735021302903448 1104 46-47 0.6953409001309723 1104 48-49 -0.37523602146524837 1104 50-51 0.16883288920364947 1104 52-53 -0.15388247173024183 1104 54-55 -0.11982449459987521 1104 56-57 -0.04026800359145 1104 58-59 -0.8947365912632819 1104 60-61 0.3116052880752207 1104 62-63 -0.07526353713167566 1104 64-65 0.30957573944034067 1104 66-67 -0.11293834719956664 1104 68-69 1.3389125469604224 1104 70-71 1.1375849509267013 1104 72-73 0.8925090164801688 1104 74-75 -0.5285508326137247 1104 76 -0.5640838742644263 1105 1 0.8591328104184583 1105 2 0.27601614365616456 1105 3 0.10106113958642027 1105 4 0.08165355427639298 1105 5 0.09023295882905558 1105 6 0.20274534406868128 1105 7 0.520999231013235 1105 8 -0.3035525606687841 1105 9 0.6950324867946023 1105 10-11 0.5942187113889403 1105 12-13 -0.2866902559405702 1105 14-15 0.3152169972625032 1105 16-17 0.1018199303836198 1105 18-19 0.6589609701748032 1105 20-21 0.33657257656747674 1105 22-23 0.26269994780568595 1105 24-25 0.7877375959580846 1105 26-27 0.5045642157487364 1105 28-29 0.9863568825171924 1105 30-31 1.0481579793802993 1105 32-33 0.9090506885073282 1105 34-35 -1.0684992515033116 1105 36-37 0.24541045749739965 1105 38-39 0.1713501982046921 1105 40-41 0.45669549404584586 1105 42-43 0.4119744006391457 1105 44-45 0.8183975012478513 1105 46-47 0.9090377929654778 1105 48-49 0.27087067251716235 1105 50-51 0.8751073798789051 1105 52-53 0.28645377203022093 1105 54-55 0.6358053120762222 1105 56-57 0.4850640940298021 1105 58-59 0.6832514743595297 1105 60-61 1.0853317729621423 1105 62-63 -0.36442016363770335 1105 64-65 0.7922351219094779 1105 66-67 0.9135969437618527 1105 68-69 -0.08648133626478227 1105 70-71 0.4815844306268815 1105 72-73 0.06513474205453207 1105 74-75 -0.3928826538257013 1105 76 0.17896931013308404 1106 1 -1.2028907516051 1106 2 0.19342140316668832 1106 3 0.09646545078020807 1106 4 -0.11977256820236448 1106 5 -0.016999779982633356 1106 6 0.26874454164683215 1106 7 -0.7314536267027805 1106 8 0.11720828114469128 1106 9 -0.680117967303218 1106 10-11 0.2224785501750901 1106 12-13 1.0875209998496302 1106 14-15 -0.3191977818891161 1106 16-17 0.4097675541207124 1106 18-19 0.4929052383295982 1106 20-21 0.3785264737845395 1106 22-23 0.5209247718199848 1106 24-25 -0.34682307807626955 1106 26-27 1.0577155452158564 1106 28-29 0.7885963689671982 1106 30-31 0.9485938403424825 1106 32-33 0.3556258061350732 1106 34-35 0.9670899814543432 1106 36-37 0.916034522858304 1106 38-39 0.4711550637990314 1106 40-41 1.0083149272442284 1106 42-43 0.901214653037286 1106 44-45 1.209103997217511 1106 46-47 0.8285494098455146 1106 48-49 1.0895962807164494 1106 50-51 0.07005941693619633 1106 52-53 0.39702385628451964 1106 54-55 0.1544889754967258 1106 56-57 0.2659026248683318 1106 58-59 0.7395030700848082 1106 60-61 1.3194474676041494 1106 62-63 0.9779221787046453 1106 64-65 -0.9159751126165432 1106 66-67 0.40274601185829084 1106 68-69 1.6318138820304355 1106 70-71 0.0995951539007649 1106 72-73 0.3660247008392261 1106 74-75 -0.22726460662870096 1106 76 -0.25457557078021864 1107 1 0.6648008266127903 1107 2 -0.2341053138337088 1107 3 0.22656721246091394 1107 4 0.25169404010635077 1107 5 0.21573903170354924 1107 6 0.3322999999391314 1107 7 0.6303109719039242 1107 8 -0.060637580911695466 1107 9 -0.24828735126207846 1107 10-11 0.6893604117937926 1107 12-13 -0.30490887942235645 1107 14-15 0.3982129486795074 1107 16-17 0.5836013069018335 1107 18-19 0.6407423466930311 1107 20-21 0.8750341150290168 1107 22-23 -0.0409437768906713 1107 24-25 0.3606120898852154 1107 26-27 -0.0035329502431622473 1107 28-29 0.8446564776588872 1107 30-31 0.49957498342888584 1107 32-33 1.1398199192765546 1107 34-35 0.2695574286586293 1107 36-37 1.2656533724771535 1107 38-39 0.7988805625771533 1107 40-41 0.8939424576086026 1107 42-43 1.077966303473147 1107 44-45 0.9034177441628373 1107 46-47 1.10944265126507 1107 48-49 1.0502228992378093 1107 50-51 1.3933260033606771 1107 52-53 0.8492068084674713 1107 54-55 0.9030117898090069 1107 56-57 1.1146187499002451 1107 58-59 0.7217130128210698 1107 60-61 1.2796637567678104 1107 62-63 0.6517741683461082 1107 64-65 0.8205752028811446 1107 66-67 -0.5762815987482739 1107 68-69 1.4297129957190222 1107 70-71 -0.2633548406281818 1107 72-73 0.7878068068318598 1107 74-75 0.09699588868442532 1107 76 0.6486049376634497 1108 1 -0.17730443654509997 1108 2 0.4177165485144698 1108 3 0.29539312339208834 1108 4 0.4500746069079753 1108 5 0.09023295882905558 1108 6 -0.07660688265196569 1108 7 -0.08628821837947243 1108 8 -1.088977661883355 1108 9 -0.19565577231470854 1108 10-11 0.09219441989096566 1108 12-13 0.9886133877841274 1108 14-15 0.41845586365926835 1108 16-17 -0.13907075787549417 1108 18-19 0.3087585410250142 1108 20-21 0.9681515239359015 1108 22-23 -0.09559964733601589 1108 24-25 1.0569683651888582 1108 26-27 -0.2383507640083522 1108 28-29 -0.3861127531103463 1108 30-31 0.41455474051390695 1108 32-33 0.558848259357525 1108 34-35 0.9213792910068079 1108 36-37 1.3607950728820128 1108 38-39 1.3251963520508383 1108 40-41 1.183416141819123 1108 42-43 0.2500310808010866 1108 44-45 0.8487618737174927 1108 46-47 0.9535722059209419 1108 48-49 1.143340308144687 1108 50-51 0.992516286761493 1108 52-53 0.30062381251605075 1108 54-55 0.01232353069969605 1108 56-57 0.5093555920055124 1108 58-59 0.3593648346834186 1108 60-61 1.158206266889266 1108 62-63 1.139628419358246 1108 64-65 1.0594415996422768 1108 66-67 1.405499777769954 1108 68-69 1.8568385017918985 1108 70-71 -0.002221237389321118 1108 72-73 0.623839195495826 1108 74-75 0.4532711923281454 1108 76 1.5514389457606157 1109 1 -0.9342915071994042 1109 2 0.3302638908156297 1109 3 0.3123547271533553 1109 4 0.3251562315651455 1109 5 0.16020161496069818 1109 6 -0.12994043365105057 1109 7 -0.44076777956532 1109 8 0.32670406725338097 1109 9 0.6652394869252021 1109 10-11 0.3983358590969175 1109 12-13 0.10813637328758574 1109 14-15 0.4145460425805183 1109 16-17 0.6412772896627033 1109 18-19 0.7566003850892642 1109 20-21 1.0211993886348623 1109 22-23 0.33627641378453177 1109 24-25 -0.038573296036169324 1109 26-27 0.31572557137103985 1109 28-29 -1.6701910474815023 1109 30-31 0.38220688887487597 1109 32-33 0.3991088052632321 1109 34-35 -0.27348325307050914 1109 36-37 0.8720429506409246 1109 38-39 0.5119862175334049 1109 40-41 0.1736375078893886 1109 42-43 0.6651822496661808 1109 44-45 0.6640044621986192 1109 46-47 0.5638552802320262 1109 48-49 0.7620388846106678 1109 50-51 -0.03792154789960733 1109 52-53 -0.21887631808340302 1109 54-55 -0.5328475545817426 1109 56-57 -0.06394335013892771 1109 58-59 0.026156659157749118 1109 60-61 1.0628360174443188 1109 62-63 0.10735402991068099 1109 64-65 0.3906581335328312 1109 66-67 0.08979192874291186 1109 68-69 -0.47978158440374585 1109 70-71 -0.42699595333550633 1109 72-73 -0.6808003500180853 1109 74-75 -1.2996999451930762 1109 76 -0.6537327925180882 1110 1 -1.0733632649347555 1110 2 -0.13584868324625177 1110 3 -0.07363934789601956 1110 4 -0.12560084545921057 1110 5 0.3771535570278459 1110 6 -0.05026630593544468 1110 7 0.3048214238579874 1110 8 0.5674381028030808 1110 9 1.0328991314281666 1110 10-11 -0.5500347794810949 1110 12-13 0.2731709354995644 1110 14-15 0.9284120728636012 1110 16-17 0.7056123785369621 1110 18-19 -0.05911719226425305 1110 20-21 0.8730015611167588 1110 22-23 -0.4536017955637277 1110 24-25 -0.34144525126986736 1110 26-27 1.1358457162031712 1110 28-29 0.39716412039209814 1110 30-31 0.3310630442402527 1110 32-33 0.8557085419320956 1110 34-35 0.697509176159258 1110 36-37 1.5808810019904982 1110 38-39 1.242960542747369 1110 40-41 -0.45392997071495245 1110 42-43 0.4809903470986967 1110 44-45 0.937087082343453 1110 46-47 0.7956113697646146 1110 48-49 1.2969138023196862 1110 50-51 -0.09484221939401039 1110 52-53 0.7074485667092318 1110 54-55 0.04458825845315317 1110 56-57 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-3.13082131449314 2116 40-41 -0.27039320672706424 2116 42-43 -0.7589862176898947 2116 44-45 -0.2103106327234343 2116 46-47 -0.48569904913978945 2116 48-49 0.42269253486533387 2116 50-51 -0.37185618287414 2116 52-53 0.2588497970578274 2116 54-55 -0.3838855234040963 2116 56-57 0.29533277271953295 2116 58-59 -1.9676502554895663 2116 60-61 -1.0295007629230284 2116 62-63 0.5757712239221036 2116 64-65 -1.6505326366477462 2116 66-67 -0.44507070437948215 2116 68-69 -0.5077179943435439 2116 70-71 0.601017629007444 2116 72-73 0.042131429577530355 2116 74-75 0.3934625798879594 2116 76 -1.4459846832419636 2117 1 0.7428700777173702 2117 2 0.6016035579241858 2117 3 0.4379439155463416 2117 4 0.045659987641151645 2117 5 0.27807611783298825 2117 6 0.24918388509987466 2117 7 0.3315477308191248 2117 8 0.35168933754355436 2117 9 -0.46988718857929257 2117 10-11 -0.19020810908691033 2117 12-13 -1.1071291297320016 2117 14-15 -0.10825738944187435 2117 16-17 -0.8136682104109454 2117 18-19 -1.283998377615113 2117 20-21 0.07607676380057882 2117 22-23 0.2653712731158038 2117 24-25 -1.3268613355117864 2117 26-27 -1.8776479004215645 2117 28-29 -1.367210122455731 2117 30-31 -0.42278095911451175 2117 32-33 -2.047403935935762 2117 34-35 0.4950117388281541 2117 36-37 -0.9707358091175067 2117 38-39 0.1536492017726161 2117 40-41 -0.18460744718499456 2117 42-43 0.34679036573628963 2117 44-45 -0.14459216469783343 2117 46-47 -0.4017048169840507 2117 48-49 -0.44909309358936156 2117 50-51 0.04667301683746672 2117 52-53 0.8161895973680657 2117 54-55 -0.44581338165495055 2117 56-57 0.19651474383661593 2117 58-59 -0.9817421477357513 2117 60-61 -0.37489413427169893 2117 62-63 -0.4854247298477503 2117 64-65 -1.1824196393350448 2117 66-67 -0.5407317124413495 2117 68-69 0.470947536232579 2117 70-71 0.11940263261234918 2117 72-73 -0.01352608282115142 2117 74-75 0.3237350231380489 2117 76 0.21679305812265426 2118 1 -0.04666183546429181 2118 2 0.15677021453312534 2118 3 -0.5553978662813464 2118 4 -0.14201727987438773 2118 5 0.010741617802814574 2118 6 0.07112180878180396 2118 7 0.5974942639191241 2118 8 0.5022938071993792 2118 9 -1.6936314808167339 2118 10-11 -0.4142440755343202 2118 12-13 -0.6714483218950207 2118 14-15 0.6448554784196503 2118 16-17 -3.0929311798555545 2118 18-19 -0.5559795495419735 2118 20-21 -0.746022083583405 2118 22-23 0.6528353167261258 2118 24-25 0.7833412271981715 2118 26-27 -2.3582565943245655 2118 28-29 -0.46968834746729016 2118 30-31 -0.675994404289824 2118 32-33 0.23200989947952166 2118 34-35 -0.30100741109238527 2118 36-37 -2.6911409345374224 2118 38-39 0.1314618463525079 2118 40-41 -0.7989537952227295 2118 42-43 0.41456941749337517 2118 44-45 -2.468873539790863 2118 46-47 0.04826802714121925 2118 48-49 0.2810278003418034 2118 50-51 -0.9789741981767079 2118 52-53 -1.6301222325355837 2118 54-55 -0.22755055301858107 2118 56-57 0.40390516485325634 2118 58-59 -0.6925622551523247 2118 60-61 -0.7359063443575948 2118 62-63 -0.9877681812588506 2118 64-65 0.07118605736400951 2118 66-67 0.42272854946197214 2118 68-69 -1.761506751378299 2118 70-71 -0.5137516731024547 2118 72-73 0.9608971062843139 2118 74-75 0.7714951663601468 2118 76 0.9419756059471709 2119 1 0.40852949216808554 2119 2 -1.407797462758058 2119 3 -0.1059722810692918 2119 4 -0.5394863996808965 2119 5 -0.40067757542513505 2119 6 -0.9307785108173974 2119 7 -0.4285919414881505 2119 8 -0.029320600678310882 2119 9 -0.5073569710128112 2119 10-11 -0.6317874805615276 2119 12-13 -0.45547052108306474 2119 14-15 -0.043826822694626344 2119 16-17 -0.2304020272253453 2119 18-19 -0.3813660918240771 2119 20-21 -1.432350579739584 2119 22-23 -0.5467090384608895 2119 24-25 0.5293030480498615 2119 26-27 -0.0038901781545703784 2119 28-29 -0.15615720813931233 2119 30-31 0.7648365536440167 2119 32-33 -0.0933010619094432 2119 34-35 0.5219389480680121 2119 36-37 -0.20512339977020844 2119 38-39 -2.194054263175005 2119 40-41 -1.2073713472655783 2119 42-43 -0.042479040656402844 2119 44-45 -0.0568604966881594 2119 46-47 -0.16926458061553262 2119 48-49 -1.0199029243709816 2119 50-51 0.556678388849285 2119 52-53 -0.6481536741871281 2119 54-55 -0.29059581517630306 2119 56-57 -1.6663310794241966 2119 58-59 -0.5654783819554723 2119 60-61 0.06275695356070088 2119 62-63 -0.2614789871671164 2119 64-65 -0.3037599562837343 2119 66-67 0.6001492974968627 2119 68-69 0.5294688899795617 2119 70-71 0.07766881579794926 2119 72-73 0.9972515158895661 2119 74-75 0.23358594981008451 2119 76 0.6590439310745779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 3.0 22 6.0 23 25.0 24 58.0 25 110.0 26 239.0 27 434.0 28 753.0 29 1203.0 30 1809.0 31 2704.0 32 4068.0 33 6149.0 34 8989.0 35 12251.0 36 16985.0 37 30994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.87684081952479 21.9561660252126 11.251181120508862 32.91581203475375 2 17.02657233066765 17.919614666635937 43.41568528035768 21.638127722338734 3 15.904219769076535 26.14021340831048 35.83231545712244 22.12325136549054 4 11.124426724435942 23.16033278790533 35.60530985688277 30.109930630775967 5 10.578230508630822 38.73844806526699 37.38563296536148 13.297688460740705 6 21.9561660252126 39.637251964693135 17.495563596137448 20.91101841395681 7 22.34564771496393 34.85400198197783 20.020280703371668 22.78006959968657 8 19.646931391302346 36.846350625705796 22.619898135558064 20.8868198474338 9 19.611209697863615 20.059459334885116 19.01200709824618 41.317323869005094 10-11 20.21559770459312 25.60957341384158 29.82358092692033 24.351247954644972 12-13 22.890691618077483 19.790394321403056 27.559862644327165 29.7590514161923 14-15 23.185107510774124 23.289967965707177 20.62236408471803 32.90256043880067 16-17 24.517757138577124 27.447512156898895 21.090203037496256 26.944527667027725 18-19 22.39577331704731 26.29001405821484 22.184323938143855 29.129888686593997 20-21 20.853402779378214 24.588048212763013 21.624876126385654 32.933672881473115 22-23 23.15284275541011 29.649005554147173 21.843239381438547 25.354912309004174 24-25 24.263096033739714 31.23804475582494 21.81673618953239 22.682123020902953 26-27 22.21370791177894 28.682215205918276 28.239150976008847 20.864925906293934 28-29 18.082666912493377 23.486437279620198 30.02062639717914 28.41026941070729 30-31 17.49095434537116 24.925675831393608 31.159687492798042 26.423682330437188 32-33 17.331359037588438 30.78288124265401 27.786868244566847 24.098891475190708 34-35 18.095342352100666 31.40340162706552 31.711645272061027 18.789610748772787 36-37 18.30621557465834 28.2328132562052 31.663824295360786 21.79714687377567 38-39 20.564172293793646 24.003825678136018 28.76230093798253 26.669701090087806 40-41 19.713765527413518 24.177248738217603 30.611762807955568 25.49722292641331 42-43 24.35355258002812 23.323961190108548 31.81996266506879 20.502523564794544 44-45 19.54552787444401 25.119840519923486 29.988937798160908 25.345693807471598 46-47 24.37717499020534 21.148394828420642 27.8957617939204 26.578668387453618 48-49 21.96365605770782 21.027978152151366 29.221497545573964 27.786868244566847 50-51 17.37284229448503 21.414579060173768 33.53229932474476 27.680279320596433 52-53 18.657670945587796 21.442234564771496 36.93450254661105 22.96559194302966 54-55 17.43333871079256 21.853610195662696 33.48851144246503 27.224539651079716 56-57 22.338157682468715 21.662902445207532 30.107049849047034 25.891890023276716 58-59 17.795741052291948 27.41063815076859 32.87548109054873 21.918139706390726 60-61 19.243621949252148 34.365997557097096 25.38544859533083 21.004931898319928 62-63 15.348228895393055 40.13389873476066 23.27441174437095 21.24346062547533 64-65 15.74404830494803 41.27238367403378 21.757968242262223 21.225599778755964 66-67 15.326911110598973 40.35917586596299 21.692862575188403 22.621050448249637 68-69 15.136203360143806 38.75573275564057 22.067940356295086 24.04012352792054 70-71 15.428890783803093 36.480491346131686 22.714963932612754 25.37565393745247 72-73 16.169251688138093 33.64234518678988 22.969048881104374 27.219354243967643 74-75 16.149662372381368 33.62333202737895 23.235233112857507 26.991772487382175 76 16.160033186605517 33.62217971468738 23.352769007397846 26.865018091309256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.5 15 3.0 16 5.0 17 5.0 18 7.0 19 13.5 20 18.0 21 18.0 22 29.0 23 42.0 24 58.5 25 73.0 26 119.5 27 219.0 28 316.0 29 360.0 30 448.5 31 636.0 32 850.0 33 965.0 34 1162.5 35 1575.0 36 2259.5 37 2729.0 38 4075.0 39 6648.0 40 8011.0 41 7714.0 42 7281.0 43 6827.5 44 6082.0 45 5668.5 46 5547.0 47 5428.5 48 5114.5 49 4650.5 50 4382.0 51 4170.5 52 3481.0 53 2749.0 54 2495.0 55 2256.5 56 1767.0 57 1329.0 58 1142.0 59 944.0 60 670.0 61 490.5 62 387.0 63 317.5 64 226.0 65 182.0 66 122.0 67 84.0 68 55.0 69 18.5 70 8.5 71 6.0 72 5.0 73 2.5 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 86782.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.24588048212763 #Duplication Level Percentage of deduplicated Percentage of total 1 62.57752695982567 25.810651978520895 2 17.083868804827627 14.092784217925377 3 7.847683969380342 9.710539051877118 4 4.020226853662625 6.632711852688346 5 2.4892440073755377 5.133553040953194 6 1.5365703749231714 3.80263188218755 7 1.014136447449293 2.9280265492844135 8 0.6956473151924903 2.295406881611394 9 0.5056713415656255 1.8771173745707637 >10 2.1623735821646086 13.211265008872807 >50 0.030731407498463426 0.9368302182480238 >100 0.02514387886237917 2.152520107856468 >500 0.00279376431804213 0.7017584291673389 >1k 0.00838129295412639 10.714203406236317 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT 4252 4.89963356456408 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT 3686 4.247424581134337 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 1360 1.5671452605378995 No Hit CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 609 0.7017584291673389 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 452 0.5208453365905372 No Hit TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT 338 0.3894816897513309 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 215 0.24774722868797677 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 183 0.21087322255767324 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 180 0.20741628448295732 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT 134 0.15440990067064597 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 134 0.15440990067064597 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 124 0.14288677375492614 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT 108 0.12444977068977436 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGT 96 0.11062201839091056 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT 89 0.10255582954990666 No Hit CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 88 0.10140351685833468 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00921850153257588 0.0 2 0.0 0.0 0.0 0.012675439607291835 0.0 3 0.0 0.0 0.0 0.01382775229886382 0.0 4 0.0 0.0 0.0 0.01613237768200779 0.0 5 0.0 0.0 0.0 0.017284690373579773 0.0 6 0.0 0.0 0.0 0.017284690373579773 0.0 7 0.0 0.0 0.0 0.017284690373579773 0.0 8 0.0 0.0 0.0 0.017284690373579773 0.0 9 0.0 0.0 0.0 0.017284690373579773 0.0 10 0.0 0.0 0.0 0.024198566523011684 0.0 11 0.0 0.0 0.0 0.02535087921458367 0.0 12 0.0 0.0 0.0 0.026503191906155653 0.0 13 0.0 0.0 0.0 0.026503191906155653 0.0 14 0.0 0.0 0.0 0.028807817289299625 0.0 15 0.0 0.0 0.0 0.031112442672443594 0.0 16 0.0 0.0 0.0 0.035721693438731536 0.0 17 0.0 0.0 0.0 0.03687400613030352 0.0 18 0.0 0.0 0.0 0.04148325689659146 0.0 19 0.0 0.0 0.0 0.04263556958816345 0.0 20 0.0 0.0 0.0 0.04378788227973543 0.0 21 0.0 0.0 0.0 0.0460925076628794 0.0 22 0.0 0.0 0.0 0.053006383812311306 0.0 23 0.0 0.0 0.0 0.059920259961743216 0.0 24 0.0 0.0 0.0 0.06568182341960314 0.0 25 0.0 0.0 0.0 0.06913876149431909 0.0 26 0.0 0.0 0.0 0.07835726302689498 0.0 27 0.0 0.0 0.0 0.10946970569933857 0.0 28 0.0 0.0 0.0 0.1451913991380701 0.0 29 0.0 0.0 0.0 0.17169459104422577 0.0 30 0.0 0.0 0.0 0.22354866216496508 0.0 31 0.0 0.0 0.0 0.27309810790256045 0.0 32 0.0 0.0 0.0 0.3387799313221636 0.0 33 0.0 0.0 0.0 0.4228987578069185 0.0 34 0.0 0.0 0.0 0.5081698969832453 0.0 35 0.0 0.0 0.0 0.6234011661404438 0.0 36 0.0 0.0 0.0 0.7881818810352377 0.0 37 0.0 0.0 0.0 1.075107741236662 0.0 38 0.0 0.0 0.0 1.4346293010071214 0.0 39 0.0 0.0 0.0 1.8125878638427324 0.0 40 0.0 0.0 0.0 2.32767163697541 0.0 41 0.0 0.0 0.0 3.008688437694453 0.0 42 0.0 0.0 0.0 3.7888041298886868 0.0 43 0.0 0.0 0.0 4.753289852734438 0.0 44 0.0 0.0 0.0 5.578345739899979 0.0 45 0.0 0.0 0.0 6.262819478693738 0.0 46 0.0 0.0 0.0 6.9426839667212095 0.0 47 0.0 0.0 0.0 7.665184024336844 0.0 48 0.0 0.0 0.0 8.242492682814408 0.0 49 0.0 0.0 0.0 8.845152220506556 0.0 50 0.0 0.0 0.0 9.436288631282984 0.0 51 0.0 0.0 0.0 10.119610057385172 0.0 52 0.0 0.0 0.0 10.940056693784426 0.0 53 0.0 0.0 0.0 11.942568735452053 0.0 54 0.0 0.0 0.0 12.8701804521675 0.0 55 0.0 0.0 0.0 13.635316079371298 0.0 56 0.0 0.0 0.0 14.369339263902653 0.0 57 0.0 0.0 0.0 15.008872807725105 0.0 58 0.0 0.0 0.0 15.563135212371229 0.0 59 0.0 0.0 0.0 16.14274849623194 0.0 60 0.0 0.0 0.0 16.697010900878063 0.0 61 0.0 0.0 0.0 17.290451937037634 0.0 62 0.0 0.0 0.0 17.859694406674194 0.0 63 0.0 0.0 0.0 18.51190339010394 0.0 64 0.0 0.0 0.0 19.137609181627525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGATG 15 0.0022179582 70.0 62 TGCGGCT 15 0.0022179582 70.0 43 TATTCAA 15 0.0022179582 70.0 12 TGCCTTA 15 0.0022179582 70.0 25 ACATGAG 15 0.0022179582 70.0 9 CTATTCA 15 0.0022179582 70.0 11 GAACATG 15 0.0022179582 70.0 7 ATGCCTT 15 0.0022179582 70.0 24 TCAAGAC 15 0.0022179582 70.0 15 GTGCGGC 15 0.0022179582 70.0 42 AAAGCCG 15 0.0022179582 70.0 70 GTCGGGG 15 0.0022179582 70.0 18 CTATACA 110 0.0 60.454544 1 TTTACAC 240 0.0 56.875 2 CGCGCCA 25 2.3786926E-4 55.999996 20 TCGTGTT 25 2.3786926E-4 55.999996 9 CGTGTTA 25 2.3786926E-4 55.999996 10 ATCGCGC 25 2.3786926E-4 55.999996 18 TCGCGCC 25 2.3786926E-4 55.999996 19 CTTTACA 250 0.0 54.600002 1 >>END_MODULE