FastQCFastQC Report
Wed 25 May 2016
SRR1780421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691519
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG300.005705686329.37282613
ATGTAGT300.005746239329.3302941
TGACGAA300.00577272129.30271532
AAGTCGC300.005782931629.292124
CGGTGGT1650.026.63690443
TATCCAC701.1450429E-625.107533
GGTCATA450.001398451724.4419121
CTTATAA752.0639964E-623.4642331
ACGAACT500.002568967422.01525324
TCCTAAG904.106314E-721.98339530
CTTAACT1004.6717105E-821.97544542
TGATTCG703.2405987E-521.97544543
CTTAATA904.1292697E-721.9706782
TTAATAC803.6561578E-621.9690883
TTTTACG956.907485E-720.82637429
TTTACAC3300.020.6391222
TTTATGA755.3938187E-520.56992519
ACTTCGG755.4452492E-520.5445934
CGAAACT1401.8553692E-1020.41758325
GGTTCTA550.00446750220.01096539
Produced by FastQC (version 0.11.5)