FastQCFastQC Report
Wed 25 May 2016
SRR1780421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691519
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT412165.960212228442024No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT315674.564878188451799RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC106911.5460168122640159No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC53750.7772743771320817No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG45540.6585502350622325No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT29360.4245725713971706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC22170.3205985663445256No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC17430.25205381197045923No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG12640.18278601166417696No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC10820.15646713973151857No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG10810.1563225305450754No Hit
CTTAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC7150.10339556830687227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAT254.4257613E-544.0256161
CCGTCTT35800.042.28252444
GATCATA404.1026033E-738.522411
GCCGTCT39600.038.2251143
CTGTGTA301.2999806E-436.6694379
CTCGAAA301.3005339E-436.66678622
CTTTACA13950.036.4513131
TTTACAC14100.036.1960832
TGGACCG250.00235122635.20266312
CTCTACG250.00235122635.20266310
ACCGGAG250.002352060535.20011523
GTATTGT250.00235289535.1975736
CGGTTGA250.00235289535.1975731
CAATTTA250.00235289535.1975735
CGACAGG250.00235289535.1975737
CCGACAG250.00235289535.1975736
CTATACA7300.034.3761631
CTTATAA1550.032.664161
CTTTATA3400.032.3507082
TTACACA15850.032.060883
Produced by FastQC (version 0.11.5)