FastQCFastQC Report
Wed 25 May 2016
SRR1780420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99205
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGG150.002219597369.9944511
CCAGATG200.006935592752.49584245
TGATTCG200.006935592752.49584259
AAACATG200.006935592752.49584223
GCCAGAT200.006935592752.49584244
GATTCGG200.006935592752.49584260
TCACGTC200.006935592752.49584245
GCACCAA450.004291356531.10864650
TTGGCTC500.007181926627.99778256
GGTCAAT500.007181926627.99778232
TTCCACG500.007181926627.99778234
TACTGCT500.007181926627.99778251
CTCGCCA500.007181926627.99778246
CAGTACA500.007181926627.9977829
TAGGAAG500.007181926627.99778220
GGATAAA657.6584105E-427.0710371
ATTTATG657.911565E-426.92094434
TCTCGGT657.911565E-426.92094443
ACTTTCC657.911565E-426.92094464
ATCATCA700.001219232324.9980269