##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99205 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.008769719268184 34.0 23.0 34.0 12.0 34.0 2 29.1419182500882 34.0 27.0 34.0 12.0 34.0 3 29.394405523915125 34.0 27.0 34.0 11.0 34.0 4 29.902262990776673 34.0 31.0 34.0 11.0 34.0 5 29.750365404969507 34.0 31.0 34.0 11.0 34.0 6 32.06997631167784 37.0 31.0 38.0 11.0 38.0 7 31.464361675318784 37.0 27.0 38.0 11.0 38.0 8 30.635230079129077 36.0 26.0 38.0 11.0 38.0 9 31.373458999042388 37.0 27.0 38.0 12.0 38.0 10-11 31.75535507282899 37.0 29.0 38.0 11.0 38.0 12-13 32.092686860541306 37.0 31.0 38.0 11.0 38.0 14-15 31.700524167128673 37.0 27.5 38.0 11.0 38.0 16-17 31.509364447356482 37.0 27.0 38.0 11.0 38.0 18-19 31.541948490499472 37.0 27.5 38.0 11.0 38.0 20-21 31.652950960133058 37.0 27.5 38.0 11.0 38.0 22-23 31.521339650219243 37.0 27.0 38.0 11.0 38.0 24-25 31.927992540698554 37.0 31.0 38.0 11.0 38.0 26-27 31.909656771332088 37.0 30.0 38.0 11.0 38.0 28-29 31.944927171009525 37.0 31.0 38.0 11.0 38.0 30-31 31.813940829595282 37.0 28.5 38.0 11.0 38.0 32-33 31.677858978882114 37.0 27.0 38.0 11.0 38.0 34-35 31.60665289047931 37.0 27.0 38.0 11.0 38.0 36-37 31.53910589184013 37.0 27.0 38.0 11.0 38.0 38-39 31.898719822589587 37.0 31.0 38.0 11.0 38.0 40-41 31.529413840028226 37.0 27.0 38.0 11.0 38.0 42-43 31.843228667909884 37.0 29.0 38.0 11.0 38.0 44-45 30.997031399627033 37.0 26.5 38.0 11.0 38.0 46-47 31.61900609848294 37.0 27.0 38.0 11.0 38.0 48-49 31.464114711960086 37.0 27.0 38.0 11.0 38.0 50-51 31.449916838869008 37.0 27.0 38.0 11.0 38.0 52-53 31.409792853182804 37.0 27.0 38.0 11.0 38.0 54-55 31.17365556171564 37.0 26.5 38.0 11.0 38.0 56-57 31.17549518673454 37.0 26.5 38.0 11.0 38.0 58-59 31.053233203971573 36.5 26.5 38.0 11.0 38.0 60-61 31.0891084118744 37.0 26.5 38.0 11.0 38.0 62-63 31.14481124943299 37.0 26.5 38.0 11.0 38.0 64-65 31.214086991583088 37.0 27.0 38.0 11.0 38.0 66-67 31.265586411975203 37.0 27.0 38.0 11.0 38.0 68-69 30.917967844362686 36.5 26.0 38.0 11.0 38.0 70-71 30.82681316465904 36.0 25.5 38.0 11.0 38.0 72-73 30.885832367320198 36.5 25.5 38.0 11.0 38.0 74-75 30.467793961997884 36.0 25.0 38.0 11.0 38.0 76 29.58740990877476 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9462797644179446 1101 2 -0.9968585734146593 1101 3 -0.3274153043726429 1101 4 -1.9317879369164714 1101 5 -0.941394252613236 1101 6 -1.2414728348267836 1101 7 -0.10481080875998572 1101 8 -0.02040929046281903 1101 9 -0.409437598694101 1101 10-11 -0.8063017991308072 1101 12-13 -0.7460168789833475 1101 14-15 0.3263058119699842 1101 16-17 -0.8778982334506402 1101 18-19 0.03894225123946171 1101 20-21 -0.3892645276543263 1101 22-23 -0.3209371083813011 1101 24-25 -0.45925536857770766 1101 26-27 -0.818820194362285 1101 28-29 -0.011010341656227496 1101 30-31 -1.25035326691388 1101 32-33 0.11808495883123626 1101 34-35 -0.1934428008989464 1101 36-37 0.15096922857401296 1101 38-39 -0.5961766520422351 1101 40-41 0.12164275632643395 1101 42-43 -0.5090622024094955 1101 44-45 0.6384260376798707 1101 46-47 -0.014177623772436476 1101 48-49 -1.1085328813367816 1101 50-51 -0.3832053980636374 1101 52-53 -0.261662926761943 1101 54-55 -0.8187117557776418 1101 56-57 -0.3540891089525289 1101 58-59 0.09333495797228508 1101 60-61 0.11747439740474164 1101 62-63 -0.5129346701833164 1101 64-65 -0.12414173616793889 1101 66-67 -0.3358600468661237 1101 68-69 -0.18556830335285213 1101 70-71 0.5262666135956344 1101 72-73 0.14336640602191508 1101 74-75 -1.4905924270115491 1101 76 -2.5898087372204657 1102 1 0.06964510287482994 1102 2 -0.6053552387202252 1102 3 -0.874866645055917 1102 4 -0.1757990979345756 1102 5 0.03541601007901818 1102 6 -0.05490008404415292 1102 7 0.7562379186117312 1102 8 1.750823865927547 1102 9 0.8754235691295023 1102 10-11 -0.17246315750837482 1102 12-13 -3.1976127684620472 1102 14-15 -0.7273385326604789 1102 16-17 -2.1934436941829105 1102 18-19 -0.988398840362553 1102 20-21 -0.2036785742039413 1102 22-23 -0.8939345903467277 1102 24-25 0.5341688181651776 1102 26-27 -4.799416015785997 1102 28-29 -0.5015081638965952 1102 30-31 0.18376950428525163 1102 32-33 -6.296514537561851 1102 34-35 -0.5099410314692463 1102 36-37 -0.038308707998737646 1102 38-39 0.033340497814847936 1102 40-41 -0.08053160055392183 1102 42-43 0.1238321762484631 1102 44-45 -4.710500394877638 1102 46-47 -0.05188008736302052 1102 48-49 0.4498072574953902 1102 50-51 -5.00248877903546 1102 52-53 -1.6420562845950748 1102 54-55 0.4626701007624412 1102 56-57 -1.7485868631379127 1102 58-59 0.5992897694599684 1102 60-61 0.6491795151398705 1102 62-63 -3.7322248566539855 1102 64-65 -0.37822503547377906 1102 66-67 -0.40114179451822807 1102 68-69 -6.8401892370672925 1102 70-71 -0.701811173256143 1102 72-73 -0.117377775276573 1102 74-75 -6.075610121308536 1102 76 -2.2648972087054275 1103 1 0.03528325154243106 1103 2 -0.7275501896318666 1103 3 -0.25197043187218426 1103 4 0.18149402969515904 1103 5 0.7054136725381142 1103 6 -1.3134321083545757 1103 7 0.5048938325902235 1103 8 1.0673269047307237 1103 9 0.6317933681009826 1103 10-11 0.35871379433827855 1103 12-13 -1.2499152462554122 1103 14-15 -0.09772188936922177 1103 16-17 -0.5750180279837522 1103 18-19 -0.4826134266178137 1103 20-21 0.2757510658147595 1103 22-23 -0.10042711021582917 1103 24-25 0.21594184294310992 1103 26-27 -0.5384587927845956 1103 28-29 0.235664580376433 1103 30-31 0.047023361227743976 1103 32-33 -4.998682606753061 1103 34-35 -1.1505148977619086 1103 36-37 0.3409573041564755 1103 38-39 0.42858944592144255 1103 40-41 0.7720280067392089 1103 42-43 0.3541968326299525 1103 44-45 -4.125676879216115 1103 46-47 0.2568506279244964 1103 48-49 0.2703425543630864 1103 50-51 0.2996968217125513 1103 52-53 -0.2305906464464087 1103 54-55 0.7081411152551951 1103 56-57 1.0971354370023398 1103 58-59 0.533254238838186 1103 60-61 0.4796785801235117 1103 62-63 0.262106606646622 1103 64-65 0.6683504670975147 1103 66-67 0.5315954658838322 1103 68-69 -2.5470616073337737 1103 70-71 0.1698870502127683 1103 72-73 1.1069969325308122 1103 74-75 -1.2840018931645432 1103 76 0.6877091494058405 1104 1 -2.2728813684376554 1104 2 -0.9273510525591817 1104 3 -0.5315090976537995 1104 4 -0.5295912673509768 1104 5 -0.17656995396924557 1104 6 -1.754050539596971 1104 7 0.21736202597444887 1104 8 0.4499743214803651 1104 9 -0.6307633306488789 1104 10-11 -0.851652396612046 1104 12-13 -1.1354667453150427 1104 14-15 -0.6294870182551584 1104 16-17 -1.0595019310703755 1104 18-19 -0.23916835526036095 1104 20-21 0.2587690987830058 1104 22-23 -0.49220164279686784 1104 24-25 -0.6019583672571294 1104 26-27 -0.4491790051974718 1104 28-29 -0.8727683214932433 1104 30-31 -1.6993257924396943 1104 32-33 -1.6157862686625641 1104 34-35 -0.842571541195916 1104 36-37 -1.2116342435811092 1104 38-39 -1.3472153997473306 1104 40-41 -0.3642979142697591 1104 42-43 0.009482262434129751 1104 44-45 -3.5036086194528657 1104 46-47 -0.7528158174787549 1104 48-49 -0.8069336930094089 1104 50-51 0.08797539235089857 1104 52-53 -1.3766659866389048 1104 54-55 -1.480555090077253 1104 56-57 -0.5159163386365577 1104 58-59 -0.28091215124921476 1104 60-61 -0.5107102903267631 1104 62-63 -0.23711897140404758 1104 64-65 -0.2535020608369898 1104 66-67 -0.48809697797057794 1104 68-69 -3.6285418595740637 1104 70-71 -1.5098479868488504 1104 72-73 -0.7806842268894769 1104 74-75 -1.751213420988421 1104 76 -0.8776465315323172 1105 1 -0.4004638919506682 1105 2 0.26755294139647745 1105 3 0.06228242463471645 1105 4 0.9097178017681671 1105 5 0.8795616668003667 1105 6 0.5084875632089556 1105 7 1.3541543559121791 1105 8 0.08846010945941885 1105 9 1.445403201409583 1105 10-11 0.8991747925081341 1105 12-13 -0.33018185356608853 1105 14-15 0.04488705359957024 1105 16-17 0.8326834113989499 1105 18-19 0.763046166793675 1105 20-21 -0.3788960133760213 1105 22-23 0.42284538323028187 1105 24-25 0.6839885363199727 1105 26-27 0.49392731791432354 1105 28-29 0.14582063716032323 1105 30-31 0.44018258366430985 1105 32-33 0.3729848018634492 1105 34-35 0.7440292977171481 1105 36-37 0.6108848403883584 1105 38-39 0.40017276688893944 1105 40-41 0.7403838458844767 1105 42-43 1.0134749154315443 1105 44-45 1.3407560732496364 1105 46-47 1.4356790891205193 1105 48-49 1.1617829263272732 1105 50-51 0.4380457359926133 1105 52-53 -0.12061513079126485 1105 54-55 -0.2649322883160572 1105 56-57 -0.2235538344029102 1105 58-59 0.12876791544697497 1105 60-61 0.5823582556441096 1105 62-63 0.7945421806684863 1105 64-65 0.5856910715393191 1105 66-67 0.6972975977530496 1105 68-69 1.2415568820563436 1105 70-71 1.5310620514493571 1105 72-73 0.7517389813016493 1105 74-75 0.051068839387269804 1105 76 0.191876434364044 1106 1 0.2951924535679247 1106 2 -0.14504626087335382 1106 3 -0.4669608282402642 1106 4 -0.47548169930781015 1106 5 -0.05969022129810142 1106 6 -0.8412389880472624 1106 7 0.4868215434335994 1106 8 -0.49094967986854243 1106 9 0.7989402163635972 1106 10-11 0.3239661079405174 1106 12-13 0.5845967146316156 1106 14-15 -0.3553829684682235 1106 16-17 0.07680666208085185 1106 18-19 -0.02216292897506733 1106 20-21 -0.2268681065267799 1106 22-23 -0.13281754150620628 1106 24-25 0.10296820927801775 1106 26-27 -0.17871547167406376 1106 28-29 -0.8422339703482074 1106 30-31 -0.35405923000675443 1106 32-33 0.9332189123668932 1106 34-35 1.0782261533992568 1106 36-37 0.5667081335610469 1106 38-39 -0.026098872569914278 1106 40-41 -0.15808374461403574 1106 42-43 0.7510669216818116 1106 44-45 0.9453245176758038 1106 46-47 0.1714001302817536 1106 48-49 0.19399368060719624 1106 50-51 0.36561265897464423 1106 52-53 0.02820540733392818 1106 54-55 0.2239652814853308 1106 56-57 0.49164827094681485 1106 58-59 0.41050236176467436 1106 60-61 0.6044603157617132 1106 62-63 0.242593772702147 1106 64-65 0.7374311376056362 1106 66-67 0.5917491239945321 1106 68-69 1.0655628033524494 1106 70-71 0.3823237535478512 1106 72-73 -0.34895731925895745 1106 74-75 -1.2414185161783422 1106 76 -0.14916006658857128 1107 1 -0.3277834357544904 1107 2 0.08275462237494224 1107 3 0.3093401050301843 1107 4 0.02885593593065039 1107 5 -0.04461211527972253 1107 6 -0.3052444761920121 1107 7 -0.04374199184631067 1107 8 0.015403133520244694 1107 9 -1.1511376752741285 1107 10-11 0.10924244460389332 1107 12-13 0.5689898029993188 1107 14-15 -0.6815774260137104 1107 16-17 0.21099465962262443 1107 18-19 0.2965139136266579 1107 20-21 0.5274659557007766 1107 22-23 -0.023476261796620435 1107 24-25 -0.20666891573448964 1107 26-27 0.39558886310276264 1107 28-29 -0.7586040771097728 1107 30-31 -0.6644109572002286 1107 32-33 0.4409223238777038 1107 34-35 0.31913284064646774 1107 36-37 -0.3855564407504346 1107 38-39 0.4642539979870399 1107 40-41 0.27802367438581044 1107 42-43 0.3479884154862063 1107 44-45 0.059653720608530136 1107 46-47 0.3331503339433759 1107 48-49 0.1205540144693309 1107 50-51 0.8700717765838704 1107 52-53 0.6410053615993938 1107 54-55 0.3881594246058562 1107 56-57 0.5343743353467652 1107 58-59 0.4826843280640354 1107 60-61 -0.3257587812669698 1107 62-63 -0.5486494921722951 1107 64-65 -0.05314754724050985 1107 66-67 0.7237147087534979 1107 68-69 0.6956275463100461 1107 70-71 -0.07427845600209437 1107 72-73 -0.0636023763556679 1107 74-75 0.15589315041686547 1107 76 -1.2257082522136322 1108 1 -1.4380786590515697 1108 2 -1.4496873604830611 1108 3 -1.0387126241563251 1108 4 -1.8178936004334396 1108 5 -1.2974440535284302 1108 6 -1.356808307578909 1108 7 -0.3918197824332488 1108 8 -0.4331834034954447 1108 9 -1.1759735231013053 1108 10-11 -0.7024211311567186 1108 12-13 -0.784845844336651 1108 14-15 -0.7758960065359908 1108 16-17 -1.5331472380266185 1108 18-19 -1.3727695809350173 1108 20-21 -1.3000552211825855 1108 22-23 -1.0729725276479591 1108 24-25 -1.9157687102310064 1108 26-27 -1.4439241938868612 1108 28-29 -1.6943466464045436 1108 30-31 -0.9820642076638642 1108 32-33 -0.8217587447404036 1108 34-35 -1.1196922809013223 1108 36-37 -1.4524954413017817 1108 38-39 -0.67903455897752 1108 40-41 -0.8346774433526498 1108 42-43 -0.9535190277497207 1108 44-45 -0.723477450761326 1108 46-47 -0.7508805417019566 1108 48-49 -0.9836888806236423 1108 50-51 -0.9755124046601757 1108 52-53 -1.0356273886386909 1108 54-55 -1.052294687969951 1108 56-57 -0.7522215746326886 1108 58-59 -1.5390564368099078 1108 60-61 -0.3873095807256419 1108 62-63 -1.3210481188075498 1108 64-65 -1.176668924615079 1108 66-67 -1.687148155263344 1108 68-69 -0.7005368063539805 1108 70-71 0.1139827840360006 1108 72-73 -0.011318029706380628 1108 74-75 -0.0626046692412956 1108 76 -0.23156008921428395 1109 1 -0.13917133611036192 1109 2 -0.7001635270912487 1109 3 0.4481301406173728 1109 4 0.6445142989199759 1109 5 0.11553023347472546 1109 6 0.40294057718876886 1109 7 -1.2323540914904392 1109 8 0.5776807135914197 1109 9 0.8595384813094995 1109 10-11 0.19287233784233138 1109 12-13 -0.47905290162701064 1109 14-15 -0.1317553619567704 1109 16-17 -0.9117811410891186 1109 18-19 -0.3369380436687166 1109 20-21 0.9046901549890336 1109 22-23 0.6064169766375436 1109 24-25 0.31646275686337333 1109 26-27 0.45786644317393765 1109 28-29 -0.13582827639802986 1109 30-31 0.28576697874282786 1109 32-33 0.5850504377567098 1109 34-35 0.5913748335290308 1109 36-37 0.4970058368296435 1109 38-39 0.764965741759287 1109 40-41 0.9061375533893674 1109 42-43 0.5686289848812294 1109 44-45 -0.18233916195374889 1109 46-47 -0.19354005751569758 1109 48-49 0.19350775112413743 1109 50-51 1.0195379687546904 1109 52-53 0.5182295608876473 1109 54-55 0.1176967911539002 1109 56-57 0.2639117018948056 1109 58-59 -0.20236905271888617 1109 60-61 0.27032662798569973 1109 62-63 -0.31021532847122657 1109 64-65 0.11233287980575213 1109 66-67 0.36606346320189687 1109 68-69 1.8966951619684096 1109 70-71 0.7193158500477281 1109 72-73 0.17839050620661112 1109 74-75 1.28934510771224 1109 76 0.002049754903804768 1110 1 0.7144519089719559 1110 2 0.20205643562693254 1110 3 0.32005536841448645 1110 4 0.14096209785887837 1110 5 -0.07827003505863672 1110 6 0.09909377156260746 1110 7 0.8180806457770373 1110 8 1.3880388015295786 1110 9 -0.7476858483347577 1110 10-11 -0.5121255112836351 1110 12-13 0.19342764273965685 1110 14-15 0.025128086741624145 1110 16-17 -0.05495113835164389 1110 18-19 0.8332808240590488 1110 20-21 -0.5163336610525775 1110 22-23 0.5198149665479868 1110 24-25 0.7677813078260556 1110 26-27 -1.2230025099370074 1110 28-29 0.31161218349794595 1110 30-31 0.5318139329748419 1110 32-33 1.2203013078830232 1110 34-35 0.571893128993942 1110 36-37 0.39476762427113954 1110 38-39 0.3648054038765274 1110 40-41 0.8983618178556263 1110 42-43 0.6166391005070011 1110 44-45 1.3774486184746024 1110 46-47 0.8507549120461704 1110 48-49 0.7265855866930764 1110 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-1.3425865090151063 2116 36-37 0.02461033058443718 2116 38-39 0.3094078602436916 2116 40-41 0.7756511951450058 2116 42-43 0.36802888719090276 2116 44-45 1.1899028309288155 2116 46-47 0.23116861598929006 2116 48-49 0.40320700449096236 2116 50-51 0.4194751678336104 2116 52-53 1.0511442214137787 2116 54-55 -0.723849507080697 2116 56-57 0.013098778860825888 2116 58-59 -0.8440688643161245 2116 60-61 -0.9662932868841629 2116 62-63 0.5662413040038281 2116 64-65 0.22212284561413753 2116 66-67 -0.4928717122917874 2116 68-69 -0.5196958784335166 2116 70-71 -3.316920281415534 2116 72-73 -0.3669587366933982 2116 74-75 -1.0540573066342809 2116 76 -0.9051723510204148 2117 1 0.8371130316330806 2117 2 0.9382211717916711 2117 3 1.0990871474462658 2117 4 0.3615343184310973 2117 5 -0.5031784599670637 2117 6 0.374512046980886 2117 7 -1.285968458272066 2117 8 -1.350402936912161 2117 9 0.8658177651688561 2117 10-11 0.45772895857083995 2117 12-13 1.0397299890419944 2117 14-15 0.0749251365386776 2117 16-17 0.5100919266914197 2117 18-19 0.3649608557390813 2117 20-21 0.19780355308463982 2117 22-23 0.4272993740323976 2117 24-25 0.9721670122117558 2117 26-27 1.4364866495521547 2117 28-29 0.8662602381460012 2117 30-31 1.1543760555369644 2117 32-33 1.6214002089819282 2117 34-35 0.6232246803254995 2117 36-37 0.047178276681790976 2117 38-39 1.0456785847497052 2117 40-41 -0.19410427461047064 2117 42-43 -0.36846138459348765 2117 44-45 1.0648063058322883 2117 46-47 -0.33541477412351384 2117 48-49 0.3429602030676939 2117 50-51 1.448205578916962 2117 52-53 1.2492440018665008 2117 54-55 -0.2006986251062841 2117 56-57 0.09891905291639347 2117 58-59 0.5526152147208983 2117 60-61 1.3056046614843737 2117 62-63 0.19259454800412712 2117 64-65 0.3564554975938492 2117 66-67 -0.912615826153548 2117 68-69 -0.32723619185618347 2117 70-71 0.8539632071150116 2117 72-73 0.9509760047707552 2117 74-75 0.48945090746216735 2117 76 0.12121874595891668 2118 1 1.3133324292007913 2118 2 0.9778723799822018 2118 3 1.0794495323030304 2118 4 1.049675107695485 2118 5 0.7509681424941448 2118 6 1.7272371637509458 2118 7 1.937478102253145 2118 8 -1.9077178762495706 2118 9 0.6700200412812443 2118 10-11 0.5170756246590784 2118 12-13 1.4489530162425552 2118 14-15 -0.13849293642995875 2118 16-17 1.2112545660563079 2118 18-19 0.9901108853834906 2118 20-21 -1.174501336074183 2118 22-23 0.9107274248761748 2118 24-25 1.249486868183297 2118 26-27 2.3355438126542616 2118 28-29 1.0322978648577852 2118 30-31 1.3749015569613405 2118 32-33 1.1973766669808548 2118 34-35 1.836946427755116 2118 36-37 1.1988998216617617 2118 38-39 1.3359335266900452 2118 40-41 0.19512685057571133 2118 42-43 0.8970440887771574 2118 44-45 1.777168748531814 2118 46-47 0.6759802485074644 2118 48-49 0.27282586327043035 2118 50-51 1.0836082367915338 2118 52-53 0.578947702356718 2118 54-55 1.2835970670545755 2118 56-57 2.2347555314495047 2118 58-59 1.714820758948914 2118 60-61 -0.5364629277742239 2118 62-63 1.0612842650084495 2118 64-65 1.3209111249708236 2118 66-67 -1.0383442833426813 2118 68-69 1.5066755690218656 2118 70-71 0.6859878481284198 2118 72-73 0.25115097910303064 2118 74-75 1.0313579830771076 2118 76 0.7334264592902251 2119 1 0.42751662203666996 2119 2 1.0244007579712573 2119 3 0.7150492634083818 2119 4 0.763062219959 2119 5 0.7599473031587003 2119 6 1.223635896104735 2119 7 1.0860904137868062 2119 8 1.933826347317126 2119 9 0.7114106107617033 2119 10-11 0.8166290674709415 2119 12-13 0.7798134791254867 2119 14-15 0.8148106264241584 2119 16-17 0.7591514257509182 2119 18-19 0.07656531734354033 2119 20-21 0.24007659535768155 2119 22-23 1.3699370666700936 2119 24-25 0.7464137864585325 2119 26-27 0.7058741689396761 2119 28-29 1.5252873971731553 2119 30-31 1.251594152755061 2119 32-33 0.7340375716192931 2119 34-35 1.3197563768571925 2119 36-37 0.41216689167040954 2119 38-39 -0.11107982200870126 2119 40-41 0.5299246994185047 2119 42-43 0.3309248147927093 2119 44-45 -0.18573819471221142 2119 46-47 0.623953935245197 2119 48-49 0.45735481746230633 2119 50-51 1.221008601719987 2119 52-53 0.1355836382061355 2119 54-55 0.010212835805177178 2119 56-57 1.3626965666939093 2119 58-59 1.4972081593138462 2119 60-61 1.0888851947649059 2119 62-63 0.6578702632798432 2119 64-65 1.0697347108730604 2119 66-67 0.8180131544754303 2119 68-69 0.05531669069669931 2119 70-71 0.40804201119381744 2119 72-73 -0.6546158508211022 2119 74-75 0.7983559663672253 2119 76 1.6391212638614334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 51.0 13 364.0 14 1039.0 15 1723.0 16 2203.0 17 2760.0 18 2769.0 19 2711.0 20 2411.0 21 1939.0 22 1634.0 23 1357.0 24 1241.0 25 1263.0 26 1337.0 27 1477.0 28 1661.0 29 2040.0 30 2500.0 31 2990.0 32 3850.0 33 5211.0 34 6942.0 35 10487.0 36 17116.0 37 20124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.67493831798475 21.605458477596486 14.525480002842958 29.19412320157581 2 20.689481376946727 22.62083564336475 32.71911697999093 23.970565999697595 3 16.20281235824807 24.384859634091026 33.84708432034675 25.565243687314148 4 11.723199435512322 19.953631369386624 35.344992691900615 32.978176503200444 5 11.538732926767805 33.63036137291467 31.81291265561212 23.01799304470541 6 25.937200745930145 36.19978831712111 18.04747744569326 19.81553349125548 7 21.97973892444937 31.09823093594073 20.276195756262286 26.645834383347612 8 22.279118996018347 33.575928632629406 19.50405725517867 24.64089511617358 9 22.18940577591855 19.25507786905902 20.250995413537623 38.3045209414848 10-11 21.61130991381483 25.900408245552143 26.708835240159267 25.779446600473765 12-13 20.34423668161887 23.89899702635956 26.708331233304772 29.048435058716798 14-15 19.61191472204022 25.719973791643568 24.02147069200141 30.6466407943148 16-17 22.006451287737512 27.741545285015878 24.475076861045313 25.776926566201304 18-19 20.074593014464998 26.875661508996522 25.1821984778993 27.86754699863918 20-21 19.343783075449828 27.573711002469636 25.237135225039058 27.845370697041478 22-23 21.752935839927424 27.24711456075803 24.5204374779497 26.479512121364852 24-25 20.523663121818455 29.221813416662467 24.319842749861397 25.934680711657677 26-27 19.575626228516708 29.430472254422664 25.587923995766342 25.405977521294286 28-29 20.54331938914369 27.747593367269797 27.25215462930296 24.456932614283556 30-31 20.525175142381936 27.792953984174183 26.999143188347364 24.682727685096516 32-33 18.993498311577035 29.659795373217072 26.522352703996777 24.824353611209112 34-35 19.938511163751826 28.799455672597148 26.276397359004083 24.985635804646943 36-37 20.40370949044907 27.89526737563631 26.444735648404816 25.256287485509805 38-39 20.1638022277103 26.79703643969558 27.513734186784937 25.525427145809182 40-41 19.543873796683638 26.50118441610806 27.50768610453102 26.447255682677284 42-43 18.435562723653042 25.857567662920218 28.67143793155587 27.035431681870875 44-45 18.171463131898594 26.62164205433194 29.00509046923038 26.201804344539088 46-47 18.375081901113855 27.594375283503858 27.942644019958674 26.08789879542362 48-49 18.28184063303261 27.89929943047225 27.35547603447407 26.463383902021064 50-51 18.181039262133964 27.730457134217023 26.724459452648556 27.364044151000456 52-53 17.738017237034423 27.48651781664231 27.05206390806915 27.72340103825412 54-55 17.334307746585353 27.400332644523967 27.073232195957864 28.192127412932816 56-57 18.214303714530516 27.030391613325943 27.236026409959173 27.519278262184365 58-59 18.138702686356535 27.174033566856508 26.71135527443173 27.975908472355226 60-61 17.71231288745527 26.688170959125046 26.64230633536616 28.957209818053524 62-63 17.992036691699006 26.972934831913715 26.267325235623208 28.767703240764074 64-65 18.442114812761453 27.422508946121667 25.190262587571187 28.94511365354569 66-67 17.756665490650672 29.124036086890783 24.35814727080288 28.76115115165566 68-69 17.90786754699864 29.248525779950608 24.045159014162593 28.79844765888816 70-71 17.85494682727685 29.775716949750517 24.126808124590493 28.242528098382135 72-73 17.857466861549316 30.016632226198276 23.9851821984779 28.14071871377451 74-75 18.35240159266166 30.121465651932866 23.75535507282899 27.77077768257648 76 19.313542664180233 29.233405574315814 23.897989012650573 27.55506274885338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 1.5 11 2.5 12 2.0 13 4.5 14 6.5 15 12.0 16 23.5 17 29.0 18 34.0 19 48.0 20 78.0 21 99.0 22 107.0 23 149.0 24 238.5 25 294.0 26 339.5 27 494.5 28 671.5 29 739.0 30 818.0 31 1059.0 32 1413.5 33 1606.0 34 1766.0 35 2142.0 36 2634.5 37 2911.0 38 3232.0 39 3907.0 40 4627.0 41 5224.0 42 5455.0 43 5729.0 44 6202.5 45 6430.0 46 6458.0 47 6581.5 48 6526.5 49 6124.0 50 5900.0 51 5584.0 52 4911.5 53 4301.5 54 4048.0 55 3771.0 56 3233.0 57 2681.5 58 2391.0 59 2119.0 60 1630.0 61 1266.5 62 1120.0 63 943.5 64 654.5 65 458.0 66 302.5 67 231.0 68 199.0 69 135.0 70 88.0 71 73.0 72 59.0 73 40.5 74 30.0 75 24.0 76 27.5 77 21.5 78 10.5 79 9.0 80 11.0 81 12.0 82 8.5 83 6.0 84 9.0 85 10.5 86 8.0 87 7.0 88 9.0 89 7.0 90 6.0 91 5.5 92 2.0 93 2.0 94 2.5 95 5.0 96 7.0 97 4.0 98 2.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7217378156342926 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99205.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.53570888564084 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60976228387841 58.38012196965878 2 9.951104034269449 14.23718562572451 3 4.0990883086505 8.796935638324682 4 1.7007905082644044 4.866690186986543 5 0.9046458212972227 3.23572400584648 6 0.5706877844632012 2.4494733128370543 7 0.3832767342567672 1.919258101910186 8 0.21559316301943157 1.2338087797994053 9 0.15922893739343638 1.0251499420392118 >10 0.4058224245071653 3.8556524368731413 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006048082253918653 0.0 2 0.0 0.0 0.0 0.006048082253918653 0.0 3 0.0 0.0 0.0 0.006048082253918653 0.0 4 0.0 0.0 0.0 0.007056095962905095 0.0 5 0.0 0.0 0.0 0.007056095962905095 0.0 6 0.0 0.0 0.0 0.007056095962905095 0.0 7 0.0 0.0 0.0 0.008064109671891537 0.0 8 0.0 0.0 0.0 0.008064109671891537 0.0 9 0.0 0.0 0.0 0.015120205634796633 0.0 10 0.0 0.0 0.0 0.016128219343783074 0.0 11 0.0 0.0 0.0 0.016128219343783074 0.0 12 0.0 0.0 0.0 0.01713623305276952 0.0 13 0.0 0.0 0.0 0.01814424676175596 0.0 14 0.0 0.0 0.0 0.0191522604707424 0.0 15 0.0 0.0 0.0 0.021168287888715286 0.0 16 0.0 0.0 0.0 0.023184315306688172 0.0 17 0.0 0.0 0.0 0.023184315306688172 0.0 18 0.0 0.0 0.0 0.029232397560606825 0.0 19 0.0 0.0 0.0 0.03225643868756615 0.0 20 0.0 0.0 0.0 0.03427246610553904 0.0 21 0.0 0.0 0.0 0.03528047981452548 0.0 22 0.0 0.0 0.0 0.0383045209414848 0.0 23 0.0 0.0 0.0 0.04233657577743057 0.0 24 0.0 0.0 0.0 0.046368630613376344 0.0 25 0.0 0.0 0.0 0.05342472657628144 0.0 26 0.0 0.0 0.0 0.06048082253918653 0.0 27 0.0 0.0 0.0 0.07761705559195604 0.0 28 0.0 0.0 0.0 0.09878534348067133 0.0 29 0.0 0.0 0.0 0.12297767249634595 0.0 30 0.0 0.0 0.0 0.16329822085580364 0.0 31 0.0 0.0 0.0 0.20563479663323422 0.0 32 0.0 0.0 0.0 0.24797137241066478 0.0 33 0.0 0.0 0.0 0.3084521949498513 0.0 34 0.0 0.0 0.0 0.392117332795726 0.0 35 0.0 0.0 0.0 0.4747744569326143 0.0 36 0.0 0.0 0.0 0.6098482939367975 0.0 37 0.0 0.0 0.0 0.8477395292575979 0.0 38 0.0 0.0 0.0 1.239856862053324 0.0 39 0.0 0.0 0.0 1.682374880298372 0.0 40 0.0 0.0 0.0 2.2236782420240915 0.0 41 0.0 0.0 0.0 2.8002620835643364 0.0 42 0.0 0.0 0.0 3.3677738017237036 0.0 43 0.0 0.0 0.0 3.8697646287989516 0.0 44 0.0 0.0 0.0 4.310266619626027 0.0 45 0.0 0.0 0.0 4.762864774960939 0.0 46 0.0 0.0 0.0 5.334408547956253 0.0 47 0.0 0.0 0.0 5.840431429867446 0.0 48 0.0 0.0 0.0 6.380726777884179 0.0 49 0.0 0.0 0.0 6.941182400080641 0.0 50 0.0 0.0 0.0 7.514742200493926 0.0 51 0.0 0.0 0.0 8.178015221007005 0.0 52 0.0 0.0 0.0 8.721334610150699 0.0 53 0.0 0.0 0.0 9.250541807368581 0.0 54 0.0 0.0 0.0 9.778740990877475 0.0 55 0.0 0.0 0.0 10.25653948893705 0.0 56 0.0 0.0 0.0 10.752482233758379 0.0 57 0.0 0.0 0.0 11.235320800362885 0.0 58 0.0 0.0 0.0 11.74940779194597 0.0 59 0.0 0.0 0.0 12.16874149488433 0.0 60 0.0 0.0 0.0 12.669724308250592 0.0 61 0.0 0.0 0.0 13.127362532130437 0.0 62 0.0 0.0 0.0 13.624313290660753 0.0 63 0.0 0.0 0.0 14.121264049191069 0.0 64 0.0 0.0 0.0 14.601078574668616 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTGG 15 0.0022195973 69.99445 11 CCAGATG 20 0.0069355927 52.495842 45 TGATTCG 20 0.0069355927 52.495842 59 AAACATG 20 0.0069355927 52.495842 23 GCCAGAT 20 0.0069355927 52.495842 44 GATTCGG 20 0.0069355927 52.495842 60 TCACGTC 20 0.0069355927 52.495842 45 GCACCAA 45 0.0042913565 31.108646 50 TTGGCTC 50 0.0071819266 27.997782 56 GGTCAAT 50 0.0071819266 27.997782 32 TTCCACG 50 0.0071819266 27.997782 34 TACTGCT 50 0.0071819266 27.997782 51 CTCGCCA 50 0.0071819266 27.997782 46 CAGTACA 50 0.0071819266 27.997782 9 TAGGAAG 50 0.0071819266 27.997782 20 GGATAAA 65 7.6584105E-4 27.071037 1 ATTTATG 65 7.911565E-4 26.920944 34 TCTCGGT 65 7.911565E-4 26.920944 43 ACTTTCC 65 7.911565E-4 26.920944 64 ATCATCA 70 0.0012192323 24.99802 69 >>END_MODULE