FastQCFastQC Report
Wed 25 May 2016
SRR1780420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99205
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT45844.620734841993851No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT42484.282042235774407RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC14991.5110125497706768No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC7520.7580263091578046No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG4510.45461418275288545No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT4140.41731767552038707No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT2660.2681316465903936No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC2490.2509954135376241No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC2000.20160274179728843No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1490.1501940426389799No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1490.1501940426389799No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1410.14212993296708834No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC1400.1411219192581019No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT1340.13507383700418327No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT1280.1290257547502646No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT1220.12297767249634595No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT1150.11592157653344086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC150.002218900770.01
GGTACCC150.002218900770.046
CGCGGTA150.002218900770.043
CGGCCGC150.002218900770.039
TTTAACG150.002218900770.034
GACAAAG150.002218900770.01
TAACGGC150.002218900770.036
GCCGCGG150.002218900770.041
AACGGCC150.002218900770.037
ACGGCCG150.002218900770.038
AGGATAT150.002218900770.028
ACGCGGA150.002218900770.025
CTATACA1400.060.01
CTTTACA2750.056.01
TTTACAC2900.053.1034472
GTCTGCA200.006933421452.517
GGCCGCG200.006933421452.540
GGATATG200.006933421452.529
TACGGGT200.006933421452.54
TTATCAC200.006933421452.52
Produced by FastQC (version 0.11.5)