FastQCFastQC Report
Wed 25 May 2016
SRR1780419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659158
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTT300.00575842129.31738530
CGGTGGT1600.027.47044843
CGACGTT609.6421245E-625.7015114
CCGACGT551.5836646E-424.01978713
CCGGAAT551.6099915E-423.9614773
CGTTACT703.167826E-522.03992517
TGACGGG904.0467057E-722.01646235
TACTCGT500.002568021422.0164640
TCGGTGG2950.021.60715742
TAACCGG856.103084E-620.70720522
ACGTTAC755.3888085E-520.57216316
GACGTTA755.3888085E-520.57216315
TTTACAC3650.020.4587042
CTTTACA3600.020.1541731
CGTGTAT550.004462117320.01496535
CGTGCGA550.004473913520.0058410
ACCGATT550.00450549419.98153942
TTAATAC1001.139264E-619.7682173
CCCGACG808.9291956E-519.26440412
CACCGAT708.168518E-418.83973741
Produced by FastQC (version 0.11.5)