FastQCFastQC Report
Wed 25 May 2016
SRR1780419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659158
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT400806.080484496888454No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT311014.718292124194806RNA PCR Primer, Index 45 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC97311.4762773113578231No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC55880.8477481878396378No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG41340.6271637452628961No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT29590.4489060286001232No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC25960.39383577230345373No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC17340.263062877185743No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11950.18129189056341574No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11170.16945861235090828No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC8960.13593099074880377No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC8950.13577928205377163No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC6950.10543754304734221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT31600.041.8375944
CTTTACA11500.038.4775161
GCCGTCT35200.037.55874343
TTTACAC12150.036.2186162
TTGCGTT250.00235317435.1964833
TGACTCG250.00235317435.1964828
CTATACA7100.034.7270971
CGTAGCG451.0346175E-634.22918315
TTACACA13350.033.457453
CTTATAC61200.030.000151
TTATACA61750.029.5746232
TTGCGAT454.063231E-529.33707616
TAGCCCG602.9406146E-729.3348525
CGTTTCG300.0057459529.330437
ATTTGCG300.0057459529.330431
AGGTCGT300.0057459529.330428
ACTTCGC703.589048E-828.2828858
AGCGGAA554.960415E-627.997218
AGCCTTA554.960415E-627.997242
ATAACAC2550.026.7485124
Produced by FastQC (version 0.11.5)