FastQCFastQC Report
Wed 25 May 2016
SRR1780418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95492
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC150.002219432569.9937164
TTAAGTC150.002219432569.9937140
GCGTGTA150.002219432569.9937134
TGGTCGC150.002219432569.9937149
TAGAGAG200.006935084252.4952855
CGGTGGT200.006935084252.49528546
AAGGGAA200.006935084252.49528515
AACTAGC200.006935084252.49528536
TCGGTGG200.006935084252.49528545
GTAGTTC200.006935084252.49528547
TCGCCGT305.8547914E-446.66247652
GTCGCCG350.001250545539.9964151
TGTTAAC350.001250545539.9964118
ATCTCGG400.002409435334.99685742
GGTGGTC400.002409435334.99685747
AAGTCCC400.002409435334.99685742
TCTCGGT552.9746475E-431.81532343
CGGATGA450.004290772631.10831533
CTTTAAC450.004290772631.10831542
ATTGAAC450.004290772631.10831527