##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95492 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.971987182172327 34.0 23.0 34.0 12.0 34.0 2 29.79929208729527 34.0 31.0 34.0 12.0 34.0 3 29.916443262262806 34.0 31.0 34.0 11.0 34.0 4 30.16183554643321 34.0 32.0 34.0 11.0 34.0 5 30.329598290956312 34.0 32.0 34.0 11.0 34.0 6 33.049375863946715 38.0 34.0 38.0 11.0 38.0 7 32.641184601851464 37.0 32.0 38.0 11.0 38.0 8 31.918631927281865 37.0 28.0 38.0 11.0 38.0 9 32.35053198173669 37.0 31.0 38.0 12.0 38.0 10-11 32.586630293637164 37.0 31.5 38.0 11.0 38.0 12-13 32.89523729736523 37.0 33.0 38.0 11.0 38.0 14-15 32.5055240229548 37.0 31.0 38.0 11.0 38.0 16-17 32.30471662547648 37.0 31.0 38.0 11.0 38.0 18-19 32.34493465421187 37.0 31.5 38.0 11.0 38.0 20-21 32.400143467515605 37.0 31.0 38.0 11.0 38.0 22-23 32.334258367193065 37.0 31.0 38.0 11.0 38.0 24-25 32.67851757215264 37.0 33.0 38.0 11.0 38.0 26-27 32.69110501403259 37.5 32.5 38.0 11.0 38.0 28-29 32.756749256482216 37.0 34.0 38.0 11.0 38.0 30-31 32.63291689356176 37.0 33.0 38.0 11.0 38.0 32-33 32.42528693503121 37.0 31.5 38.0 11.0 38.0 34-35 32.35516064172915 37.0 31.0 38.0 11.0 38.0 36-37 32.34156788003183 37.0 31.0 38.0 11.0 38.0 38-39 32.71221149415658 37.0 34.0 38.0 11.0 38.0 40-41 32.34482469735685 37.0 31.0 38.0 11.0 38.0 42-43 32.64350416788841 37.0 33.0 38.0 11.0 38.0 44-45 31.82032002680853 37.0 29.0 38.0 11.0 38.0 46-47 32.47741695639425 37.0 31.5 38.0 11.0 38.0 48-49 32.33221631131404 37.0 31.0 38.0 11.0 38.0 50-51 32.24997381979642 37.0 31.0 38.0 11.0 38.0 52-53 32.28552130021363 37.0 31.0 38.0 11.0 38.0 54-55 32.010519205797344 37.0 31.0 38.0 11.0 38.0 56-57 32.01919532526285 37.0 30.5 38.0 11.0 38.0 58-59 31.91619716834918 37.0 29.0 38.0 11.0 38.0 60-61 31.975018849746576 37.0 31.0 38.0 11.0 38.0 62-63 31.99691073597788 37.0 31.0 38.0 11.0 38.0 64-65 32.09556821513844 37.0 31.0 38.0 11.0 38.0 66-67 32.085897247937 37.0 31.0 38.0 11.0 38.0 68-69 31.751356134545304 37.0 29.0 38.0 11.0 38.0 70-71 31.666987810497215 37.0 28.0 38.0 11.0 38.0 72-73 31.700330917773215 37.0 28.5 38.0 11.0 38.0 74-75 31.290626439911197 37.0 27.0 38.0 11.0 38.0 76 30.503968918862313 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.3607150600690616 1101 2 -0.4461389365024999 1101 3 0.6908557419208705 1101 4 -1.2808576321228458 1101 5 0.010275626505261926 1101 6 -0.5911410118490252 1101 7 -0.8222928852716151 1101 8 0.253823252803663 1101 9 -0.11116544727442346 1101 10-11 -0.4258739082978238 1101 12-13 -0.9911462611737676 1101 14-15 -0.0984453732330266 1101 16-17 -0.7653416800081985 1101 18-19 0.5205515301809172 1101 20-21 -0.32766718126847394 1101 22-23 0.19751183036427733 1101 24-25 -0.014539071730737874 1101 26-27 -0.6394762860257401 1101 28-29 0.4329115322461732 1101 30-31 -0.3397884146201662 1101 32-33 0.6954267957764557 1101 34-35 -0.2691583293386941 1101 36-37 0.5323320947779848 1101 38-39 0.16986517503281817 1101 40-41 0.6756969531224897 1101 42-43 0.20071691519275703 1101 44-45 0.6051487220254721 1101 46-47 0.9582336905199611 1101 48-49 -0.9637088469196442 1101 50-51 -0.015182079065773735 1101 52-53 0.321192107200055 1101 54-55 -0.8715093021567597 1101 56-57 -0.35639934443218735 1101 58-59 0.6555497567631967 1101 60-61 0.7472556314580956 1101 62-63 -0.3673542677189019 1101 64-65 -0.1651846442336975 1101 66-67 -0.31692414605143426 1101 68-69 0.23591971168721315 1101 70-71 1.1717152620665594 1101 72-73 -0.03156971840471101 1101 74-75 -0.8643703811579471 1101 76 -1.350724007203194 1102 1 0.4542464012338634 1102 2 0.3151851053093466 1102 3 -0.09316917625038812 1102 4 0.40745405618884334 1102 5 0.2731834503886965 1102 6 0.6300077580802323 1102 7 0.9897121828310134 1102 8 1.726476615700932 1102 9 1.6713812742966923 1102 10-11 0.751905629237811 1102 12-13 -2.9804293358226914 1102 14-15 -0.27089284446098105 1102 16-17 -1.6458981154426837 1102 18-19 0.04990429124099194 1102 20-21 0.9898811240847039 1102 22-23 0.1928132768852322 1102 24-25 0.7874089802173074 1102 26-27 -3.762219396573734 1102 28-29 0.20959826015241134 1102 30-31 0.9618428809135651 1102 32-33 -5.761890277394279 1102 34-35 -0.5993240985288502 1102 36-37 0.3517809386170754 1102 38-39 0.7006169435894734 1102 40-41 0.5174507278030944 1102 42-43 0.647633819426737 1102 44-45 -4.780766176084544 1102 46-47 0.40818617705780724 1102 48-49 0.21483618528392157 1102 50-51 -4.375202140305348 1102 52-53 -0.6736869974351478 1102 54-55 1.1920003589138801 1102 56-57 -0.5556919217735441 1102 58-59 1.368198896241605 1102 60-61 0.4186736443395205 1102 62-63 -3.533941745281986 1102 64-65 0.24968706941849916 1102 66-67 0.12811861606449781 1102 68-69 -6.308162222638622 1102 70-71 0.3433972386265829 1102 72-73 0.74606727874448 1102 74-75 -5.62102226585861 1102 76 -1.362338409061497 1103 1 0.4708329539607057 1103 2 0.059769996413734816 1103 3 0.6350368649341114 1103 4 0.4451669515659695 1103 5 0.6954432569283675 1103 6 -0.41515276651771416 1103 7 1.2325464359910612 1103 8 1.6486474067519055 1103 9 0.8683428789492353 1103 10-11 1.0073504099629886 1103 12-13 -0.23473175110962075 1103 14-15 -0.2889926604956443 1103 16-17 -0.1191192892602615 1103 18-19 0.44366430548347324 1103 20-21 0.1635800143582884 1103 22-23 -0.01689237856547976 1103 24-25 1.02341871257741 1103 26-27 0.04948317300670624 1103 28-29 1.092958298132352 1103 30-31 0.8370134939335685 1103 32-33 -4.617225272350069 1103 34-35 -0.6466506666850407 1103 36-37 0.5712931337390259 1103 38-39 0.7982817706027063 1103 40-41 1.0293639673734418 1103 42-43 1.2744363830728105 1103 44-45 -3.1478215437185106 1103 46-47 0.41871967655338693 1103 48-49 0.571728951291874 1103 50-51 0.5778125175784581 1103 52-53 0.6079728447107158 1103 54-55 0.7482729836839255 1103 56-57 1.5558593214761132 1103 58-59 1.1159883449390087 1103 60-61 0.8951290475796867 1103 62-63 0.21715611241191368 1103 64-65 0.6059863988351495 1103 66-67 0.44832216481530907 1103 68-69 -2.395174862838015 1103 70-71 1.1371780231497617 1103 72-73 2.148684334112115 1103 74-75 -0.5617824581320647 1103 76 0.03909177312353407 1104 1 -1.487210611761462 1104 2 -0.33102832532889437 1104 3 0.2730045642271719 1104 4 0.19748030290800145 1104 5 -0.7935216261108877 1104 6 -0.38897310101969396 1104 7 1.058369759170553 1104 8 1.0601940473634386 1104 9 0.3378238836046705 1104 10-11 -0.25175930898637233 1104 12-13 -0.3504280554949304 1104 14-15 0.16672318524262408 1104 16-17 -0.4514803444933264 1104 18-19 0.4482622708837809 1104 20-21 0.869626338859689 1104 22-23 0.42570489714002235 1104 24-25 0.6056504500335862 1104 26-27 1.1296889319583698 1104 28-29 0.2653245260764834 1104 30-31 -0.28726088965169083 1104 32-33 -0.9653343590791934 1104 34-35 -0.04135725901793563 1104 36-37 0.49452148019572206 1104 38-39 -0.26084519071691403 1104 40-41 0.6808525586717948 1104 42-43 0.9465583441050569 1104 44-45 -2.6779238484486676 1104 46-47 0.39034160884835245 1104 48-49 0.8852958229511643 1104 50-51 1.414872118704956 1104 52-53 0.5025501192667221 1104 54-55 -0.11236555876104859 1104 56-57 0.7334701037049811 1104 58-59 0.8087436757051414 1104 60-61 0.6257026437633755 1104 62-63 0.8615034886979132 1104 64-65 0.10275345441305461 1104 66-67 -0.09630683685144703 1104 68-69 -3.9739006516764555 1104 70-71 -0.2539076714303867 1104 72-73 0.6055353025585752 1104 74-75 0.027719812113350173 1104 76 0.596595077911168 1105 1 -0.8965725848971751 1105 2 0.11776162372159149 1105 3 -0.22082938667602292 1105 4 0.2885815071692335 1105 5 0.30235609505632155 1105 6 0.2994243051868821 1105 7 1.018854937493856 1105 8 -0.21108316430863283 1105 9 0.044169413942789504 1105 10-11 0.2734114876787501 1105 12-13 -1.7830357442397187 1105 14-15 -0.3471721366656695 1105 16-17 0.42024846514076586 1105 18-19 -0.08531809039458693 1105 20-21 -0.30982595386842604 1105 22-23 0.21028398468055087 1105 24-25 -0.10474788252778922 1105 26-27 -0.5250123186205968 1105 28-29 -1.1828575026405161 1105 30-31 0.06934228311347113 1105 32-33 0.2493483644039003 1105 34-35 0.22274070300152005 1105 36-37 0.42607254550372886 1105 38-39 0.39478241465594266 1105 40-41 0.24590003435498176 1105 42-43 -0.13173311027196632 1105 44-45 1.4127865498838865 1105 46-47 0.2883123128779985 1105 48-49 -0.06240855407523327 1105 50-51 -0.47600618143399487 1105 52-53 -0.9715408950917741 1105 54-55 -1.058014586088511 1105 56-57 -0.24060643635986878 1105 58-59 -0.3595778852026861 1105 60-61 -0.175435035717868 1105 62-63 0.004900315932744093 1105 64-65 -0.3676610893572061 1105 66-67 0.16253695657142941 1105 68-69 0.7807455965866303 1105 70-71 1.246613403141172 1105 72-73 0.6334417407243897 1105 74-75 -1.8874318784841506 1105 76 -1.5816413741499709 1106 1 -0.6979970249660887 1106 2 0.08085847334010055 1106 3 -0.8416299035354875 1106 4 -0.6441498489715478 1106 5 0.11136421713360178 1106 6 0.31240732266165594 1106 7 0.6837115705236343 1106 8 1.3858126023662365 1106 9 1.7571893499506643 1106 10-11 0.34814844313038407 1106 12-13 0.29800043592272374 1106 14-15 0.3880604500928797 1106 16-17 0.7486234343753253 1106 18-19 0.660374747409989 1106 20-21 -0.20425433191419984 1106 22-23 0.7628544375478867 1106 24-25 0.743824600858872 1106 26-27 0.6704097832745504 1106 28-29 -0.8898166461305479 1106 30-31 0.12732745416934677 1106 32-33 0.639638790197651 1106 34-35 1.0219807965284673 1106 36-37 0.761669702776679 1106 38-39 0.38339920028232655 1106 40-41 0.36079358591786814 1106 42-43 0.6839895700135301 1106 44-45 1.1283061782373203 1106 46-47 0.9459621405915044 1106 48-49 0.6271911874950291 1106 50-51 0.6304191815982492 1106 52-53 0.31200882186613654 1106 54-55 0.7611230585090709 1106 56-57 0.5132777009770777 1106 58-59 -0.13273657336414146 1106 60-61 0.3140201082644154 1106 62-63 0.559885856173949 1106 64-65 0.4247644582677026 1106 66-67 1.0138552558576777 1106 68-69 0.6817347056193661 1106 70-71 0.21630944055194945 1106 72-73 0.4685389595282352 1106 74-75 -1.074716520374377 1106 76 0.06549436299007283 1107 1 -0.5732077912185076 1107 2 -0.15918086969368517 1107 3 0.5067276809271384 1107 4 0.01690679250724969 1107 5 -0.1879863105626427 1107 6 0.10758936225448679 1107 7 0.3542917241488155 1107 8 1.0384229814332429 1107 9 -0.9955584174882866 1107 10-11 0.30625279584380394 1107 12-13 0.5584919115987361 1107 14-15 -0.40643464764021076 1107 16-17 0.653731783841387 1107 18-19 0.7733298463920661 1107 20-21 1.033731991697863 1107 22-23 -0.10131061584623069 1107 24-25 0.1798219155406926 1107 26-27 0.43087003432057713 1107 28-29 -0.5360181698924933 1107 30-31 0.22723846397387604 1107 32-33 1.3403272932888868 1107 34-35 0.48925738430090604 1107 36-37 0.10860656657484213 1107 38-39 0.5268061196603284 1107 40-41 0.7875484101232075 1107 42-43 0.7896068809483978 1107 44-45 0.24940309948881279 1107 46-47 0.971285315511885 1107 48-49 0.3983849050994763 1107 50-51 1.3243943892420376 1107 52-53 1.2837817563568663 1107 54-55 0.9033824727350179 1107 56-57 0.9405008946322297 1107 58-59 0.8079210173434141 1107 60-61 0.5981876596289339 1107 62-63 0.19028771716189397 1107 64-65 0.2552537482196158 1107 66-67 1.257274665087678 1107 68-69 1.3587471330221028 1107 70-71 0.9442648517803285 1107 72-73 0.883990467677858 1107 74-75 0.2489267694526376 1107 76 -0.01134945141072663 1108 1 -0.9831607147727794 1108 2 -0.6765536830462757 1108 3 -0.8058625935071433 1108 4 -0.40258178610506334 1108 5 -0.05796437721861025 1108 6 0.3396414673205541 1108 7 0.380611736987845 1108 8 1.1867004114872728 1108 9 -0.33259634612467437 1108 10-11 -0.5282106396668169 1108 12-13 -0.3616252445559738 1108 14-15 0.004240471388300193 1108 16-17 -0.5376500168326643 1108 18-19 -0.39519580492125783 1108 20-21 -0.16288572616443275 1108 22-23 -0.9290068122833972 1108 24-25 -1.1955659301769543 1108 26-27 -0.8728934065396388 1108 28-29 -0.27064476808433113 1108 30-31 -0.17009609976656037 1108 32-33 0.06639453771192905 1108 34-35 -0.407174435096735 1108 36-37 -0.4645491601677847 1108 38-39 -0.19139721848763713 1108 40-41 -0.32097483387187964 1108 42-43 0.04305737043644342 1108 44-45 -0.12528929873512595 1108 46-47 -0.004481014130227834 1108 48-49 -0.4678283832972454 1108 50-51 0.03425235746544075 1108 52-53 -0.3837140977061537 1108 54-55 0.49478032219738566 1108 56-57 0.5221557921874691 1108 58-59 -1.086036613855434 1108 60-61 0.24453254808985037 1108 62-63 -0.5836182904345364 1108 64-65 0.39032436288820804 1108 66-67 -0.02359919143616196 1108 68-69 0.2817931930445674 1108 70-71 0.9439305020237363 1108 72-73 -0.09373515153788858 1108 74-75 0.7175781362744438 1108 76 -0.20069840556468677 1109 1 -0.4759331752871745 1109 2 -0.3362488266123904 1109 3 0.06215111321624178 1109 4 -0.06141041267562741 1109 5 0.34760496383460193 1109 6 0.4078599871519728 1109 7 0.019864956886223695 1109 8 0.6947156936961036 1109 9 0.8368865007775241 1109 10-11 1.004362521938603 1109 12-13 -0.13700040702791227 1109 14-15 -0.2254850002726485 1109 16-17 -0.4786283932949118 1109 18-19 0.35138736101674084 1109 20-21 0.8892825356812466 1109 22-23 0.5541551870076304 1109 24-25 0.7681782109865409 1109 26-27 0.5544964079469494 1109 28-29 -0.08656898723435091 1109 30-31 0.18901611418436204 1109 32-33 0.5305916309412879 1109 34-35 0.3725333123529566 1109 36-37 0.4705605330064273 1109 38-39 0.5606410658087171 1109 40-41 0.4724002498257889 1109 42-43 0.3257585402343892 1109 44-45 -0.11030249342573839 1109 46-47 -0.6966114643251977 1109 48-49 0.0937337105229119 1109 50-51 0.28085521697152416 1109 52-53 0.7799000659080093 1109 54-55 -0.2815992122466682 1109 56-57 -0.0020869901198352636 1109 58-59 0.06314216435560027 1109 60-61 0.5248114090138785 1109 62-63 -0.508546408911041 1109 64-65 -0.06312004216909628 1109 66-67 0.10082477169748216 1109 68-69 1.1320236077911083 1109 70-71 -0.13980654945525117 1109 72-73 -0.7307039175389107 1109 74-75 0.7027291859416209 1109 76 -0.10530276178194953 1110 1 -0.44132410619534923 1110 2 -0.34816411438974626 1110 3 -0.5342027245169056 1110 4 -0.40097506258510407 1110 5 -0.3753540694692603 1110 6 -0.21257704707816316 1110 7 -0.0540687722199138 1110 8 1.144802005851382 1110 9 -1.3486635813242813 1110 10-11 -1.8359599907976971 1110 12-13 -1.0628982639602498 1110 14-15 -0.9580015258926267 1110 16-17 -0.8926741176854662 1110 18-19 -0.24787161755257614 1110 20-21 -0.639032061598872 1110 22-23 -0.3305023010981287 1110 24-25 -0.5315565370240662 1110 26-27 -1.4436510384763572 1110 28-29 -0.7524146358386261 1110 30-31 -0.5892037502423975 1110 32-33 0.0709057229795178 1110 34-35 -0.07161640765883703 1110 36-37 -0.8175957551498598 1110 38-39 0.1052286621204459 1110 40-41 -0.40038405399300103 1110 42-43 0.165786328538033 1110 44-45 0.77997581064567 1110 46-47 -0.8085225417535113 1110 48-49 -0.08499560613743995 1110 50-51 -0.4160628075327111 1110 52-53 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2116 34-35 -0.17076218995894266 2116 36-37 0.9196537894767332 2116 38-39 0.8852597531323241 2116 40-41 0.1972621365729168 2116 42-43 0.8003727238649674 2116 44-45 2.0336580542233342 2116 46-47 0.33854736591560197 2116 48-49 0.9927083697434753 2116 50-51 0.3602703373702454 2116 52-53 1.214874244734652 2116 54-55 0.641087390767801 2116 56-57 0.39569419044823206 2116 58-59 -0.1619223885778034 2116 60-61 -0.40979496821545425 2116 62-63 0.7765673843994776 2116 64-65 1.0917169241432774 2116 66-67 0.4647006499176243 2116 68-69 1.1796426554101593 2116 70-71 -2.4161156228954717 2116 72-73 0.45938195287483197 2116 74-75 -1.5439530935275414 2116 76 -0.6764460993187278 2117 1 -0.13430291799426897 2117 2 0.17692474680214687 2117 3 0.4143731883298827 2117 4 0.1946026505663525 2117 5 -0.2242208007622466 2117 6 -0.3024537954509654 2117 7 -1.9584218165812715 2117 8 -1.8516897081309835 2117 9 0.5804321117930158 2117 10-11 -0.21912483407886896 2117 12-13 0.6669406318528885 2117 14-15 -0.21493731502616953 2117 16-17 0.044938401356219515 2117 18-19 0.1453103055421039 2117 20-21 0.6411692032305609 2117 22-23 0.45860816374976565 2117 24-25 0.8044772533096847 2117 26-27 1.0021551748828301 2117 28-29 0.4292084080611218 2117 30-31 0.2824668401652133 2117 32-33 0.8761496873427177 2117 34-35 0.03580177080213076 2117 36-37 -0.6977831714818592 2117 38-39 0.4814230950244749 2117 40-41 -0.612312226452957 2117 42-43 -0.708400072237481 2117 44-45 1.047228730370545 2117 46-47 0.2274143090981653 2117 48-49 -0.539987989463846 2117 50-51 0.9865130129907627 2117 52-53 1.238106518090369 2117 54-55 -0.48818379232522346 2117 56-57 0.21857588114544768 2117 58-59 -0.4971649983611961 2117 60-61 0.7227680708259641 2117 62-63 0.45643439341915837 2117 64-65 0.3001572427949313 2117 66-67 -0.9158132087959174 2117 68-69 0.04912056705674672 2117 70-71 0.6535647378919371 2117 72-73 1.2417573561271844 2117 74-75 -0.024465306313111057 2117 76 -1.1797544145468528 2118 1 0.28954873863223796 2118 2 0.11040868254512048 2118 3 -0.23277791608778742 2118 4 0.03766238952217549 2118 5 0.21389102152690853 2118 6 0.5351779816574691 2118 7 0.575840231611501 2118 8 -3.029557429014517 2118 9 -0.19640963017485547 2118 10-11 -0.6300811593800759 2118 12-13 -0.10093556041618967 2118 14-15 -0.4371307763715535 2118 16-17 -0.3871963878004081 2118 18-19 -0.921370480100471 2118 20-21 -2.6836770568096 2118 22-23 -0.8952996550253332 2118 24-25 -0.5635024781160212 2118 26-27 -0.16092684067942287 2118 28-29 -0.4624990467581611 2118 30-31 -0.43548308453358686 2118 32-33 0.4252445041097843 2118 34-35 0.325107430942694 2118 36-37 0.0732462587390259 2118 38-39 -0.7105714456381733 2118 40-41 -1.1675880171156052 2118 42-43 -0.7638562720366764 2118 44-45 0.02549335135448061 2118 46-47 -0.6017382385925956 2118 48-49 -1.3984609437811173 2118 50-51 -0.27667254172811084 2118 52-53 -1.3008147921505966 2118 54-55 -0.12396127726498207 2118 56-57 0.8615305121695442 2118 58-59 0.11721439420908197 2118 60-61 -1.654639998953158 2118 62-63 -0.19925869142019792 2118 64-65 -0.2943614570042641 2118 66-67 -1.5052020140168025 2118 68-69 0.31636140541015934 2118 70-71 -0.624939905682357 2118 72-73 -1.5166042151579582 2118 74-75 0.4296637097511464 2118 76 -0.20916894767938388 2119 1 0.21111399193620173 2119 2 0.9174020746156053 2119 3 0.5042127226787336 2119 4 0.11083144238561005 2119 5 0.5495703992802135 2119 6 0.49707913804513737 2119 7 -1.0490221031902607 2119 8 0.1601107978577332 2119 9 -0.0991319594700073 2119 10-11 -0.6461191549747909 2119 12-13 0.35773906788777765 2119 14-15 -0.5142490996094367 2119 16-17 0.5400819712304283 2119 18-19 -0.47486201369078174 2119 20-21 -0.5280981746510065 2119 22-23 0.6157565812081458 2119 24-25 -0.15275254694861928 2119 26-27 -0.23542952515079207 2119 28-29 0.7338631503784043 2119 30-31 0.3173906764796328 2119 32-33 0.36796717756793385 2119 34-35 0.4663433202598739 2119 36-37 -0.338398496216918 2119 38-39 0.013401227819976214 2119 40-41 -0.3887041377103202 2119 42-43 -0.43034512115561796 2119 44-45 -0.9279672075662226 2119 46-47 -0.37341190324017504 2119 48-49 -0.5214734024815542 2119 50-51 -0.02905587047260383 2119 52-53 -1.2422554584501562 2119 54-55 -1.5393488047980242 2119 56-57 0.5558191272576494 2119 58-59 1.0319824283500516 2119 60-61 -0.2196093684465481 2119 62-63 -0.344469663336497 2119 64-65 0.08482012339221257 2119 66-67 0.8415422040448703 2119 68-69 -0.13533531815812339 2119 70-71 -0.6447120697792741 2119 72-73 -2.2651071061271217 2119 74-75 -0.4474701008215405 2119 76 0.454394515977004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 6.0 12 51.0 13 310.0 14 883.0 15 1348.0 16 1722.0 17 2150.0 18 2262.0 19 2147.0 20 2030.0 21 1574.0 22 1237.0 23 1042.0 24 1037.0 25 994.0 26 1084.0 27 1245.0 28 1473.0 29 1784.0 30 2187.0 31 2760.0 32 3441.0 33 4770.0 34 6680.0 35 10081.0 36 17384.0 37 23809.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.23538971317617 24.74304045925582 9.868934805090648 28.152635022477362 2 22.197671009089767 23.180999455451765 29.815063041930213 24.806266493528252 3 19.80814945911133 24.406488569603418 28.36288236587741 27.42247960540784 4 16.07045616386713 19.112595819545092 34.32538851422109 30.491559502366687 5 12.573959849619337 33.90267145594873 35.43998910892126 18.083379585510677 6 23.927909436491397 35.71331329654103 18.760930349457016 21.59784691751055 7 24.790558371381895 33.60281489548863 20.349348636534998 21.25727809659448 8 21.765174045993383 37.32145101160307 18.34289783437356 22.570477108029994 9 21.994512629330206 21.636367444393247 19.60687806308382 36.76224186319273 10-11 21.91335399824069 26.908013236710925 26.30220332593306 24.87642943911532 12-13 21.933774557030954 24.870146190256776 26.010555858082352 27.185523394629918 14-15 20.71691869475977 26.10271017467432 23.195136765383488 29.98523436518242 16-17 21.586101453524904 28.242156411008253 24.598395677124785 25.573346458342062 18-19 20.477108029992042 27.69341934402882 25.51470280232899 26.314769823650145 20-21 20.14776106899007 28.033238386461694 25.511561177899722 26.307439366648516 22-23 22.197671009089767 27.444183805973278 25.02094416286181 25.337201022075146 24-25 21.078205504126 28.63224144430947 24.693691618146023 25.595861433418506 26-27 19.515247350563396 29.36581074854438 26.10375738281741 25.01518451807481 28-29 20.823733925355004 27.925899551794913 27.471411217693632 23.77895530515645 30-31 20.62633519038244 27.85783102249403 27.379256901101662 24.136576886021864 32-33 19.719976542537594 29.21815440036862 26.47132744104218 24.590541616051606 34-35 20.911699409374606 28.1594269677041 26.645163992795208 24.283709630126086 36-37 20.606961839735266 27.66985716080928 26.79857998575797 24.924601013697483 38-39 20.256670715871486 27.287626188581243 27.73740208603862 24.71830100950865 40-41 19.808465630628742 26.561387341347963 27.560947513927868 26.06919951409542 42-43 18.992166883089684 26.163971851045115 28.587211494156577 26.256649771708624 44-45 18.676957232019436 26.31529342772169 28.927030536589456 26.080718803669416 46-47 18.953420181795334 26.614794956645582 28.564696519080137 25.86708834247895 48-49 18.18162778033762 27.460939136262724 28.422276211619824 25.935156871779835 50-51 18.11722447953755 27.383969337745572 27.90547899300465 26.59332718971223 52-53 17.634461525572824 27.209609181920996 28.015435848029153 27.140493444477027 54-55 17.154840196037362 27.652054622376742 27.632157667658024 27.560947513927868 56-57 17.821388179114482 27.50020944162862 27.4143383738952 27.264064005361703 58-59 17.808298077325848 26.88759267792066 27.496544213127798 27.807565031625685 60-61 17.692581577514346 26.414254597243747 27.53529091442215 28.357872910819754 62-63 17.962237674360157 26.905918820424745 26.956708415364638 28.175135089850457 64-65 18.572236417710382 27.285008168223516 25.61471117999414 28.528044234071963 66-67 18.2277049386336 28.199744481213084 24.926695429983663 28.64585515016965 68-69 18.100469149248106 28.046328488250328 24.551271310685713 29.30193105181586 70-71 18.47118083190215 28.66575210488837 24.284756838269175 28.578310224940306 72-73 18.72303439031542 29.146944246638462 23.95383906505257 28.176182297993552 74-75 18.955514598081518 29.89308004859046 23.47212331923093 27.679282034097096 76 19.373350647174632 29.815063041930213 23.566372052109077 27.245214258786078 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 3.0 11 3.5 12 3.0 13 4.0 14 3.5 15 4.0 16 10.0 17 14.0 18 15.5 19 25.0 20 36.5 21 40.0 22 52.5 23 88.0 24 113.5 25 116.0 26 178.5 27 301.5 28 421.0 29 480.0 30 589.5 31 798.5 32 1115.0 33 1332.0 34 1556.0 35 2059.0 36 2572.0 37 2806.0 38 3150.0 39 3915.5 40 4826.0 41 5599.0 42 5883.0 43 6030.0 44 6636.5 45 6990.0 46 6884.0 47 6952.0 48 6739.5 49 6248.0 50 6037.0 51 5657.5 52 4794.5 53 4002.5 54 3694.0 55 3322.5 56 2614.0 57 2037.0 58 1797.0 59 1529.5 60 1128.5 61 881.0 62 767.0 63 663.0 64 490.0 65 358.5 66 262.5 67 229.0 68 200.0 69 137.5 70 101.0 71 98.0 72 73.5 73 41.0 74 30.0 75 27.0 76 21.5 77 12.0 78 9.0 79 10.0 80 7.5 81 4.5 82 6.5 83 9.0 84 10.0 85 6.0 86 2.0 87 3.0 88 5.0 89 5.0 90 3.0 91 6.5 92 10.0 93 6.0 94 2.5 95 3.0 96 3.0 97 4.5 98 6.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7644619444560801 2 0.0 3 0.0010472081430905208 4 0.0 5 0.0010472081430905208 6 0.0010472081430905208 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95492.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.472416537511 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4350778026833 51.21371423784191 2 9.883702043335607 12.744523101411637 3 4.187376149173245 8.099107778662088 4 2.1148036253776437 5.453860009215432 5 1.289672871390053 4.157416328069368 6 0.8332521196764447 3.2233066644326227 7 0.5506285937043174 2.4850249235538056 8 0.40606828444271187 2.0944162861810414 9 0.30536334990091935 1.7718761781091612 >10 0.9940551603157587 8.756754492522933 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0020944162861810415 0.0 2 0.0 0.0 0.0 0.0020944162861810415 0.0 3 0.0 0.0 0.0 0.0020944162861810415 0.0 4 0.0 0.0 0.0 0.004188832572362083 0.0 5 0.0 0.0 0.0 0.010472081430905207 0.0 6 0.0 0.0 0.0 0.011519289573995728 0.0 7 0.0 0.0 0.0 0.011519289573995728 0.0 8 0.0 0.0 0.0 0.011519289573995728 0.0 9 0.0 0.0 0.0 0.013613705860176769 0.0 10 0.0 0.0 0.0 0.013613705860176769 0.0 11 0.0 0.0 0.0 0.01570812214635781 0.0 12 0.0 0.0 0.0 0.01570812214635781 0.0 13 0.0 0.0 0.0 0.016755330289448332 0.0 14 0.0 0.0 0.0 0.016755330289448332 0.0 15 0.0 0.0 0.0 0.016755330289448332 0.0 16 0.0 0.0 0.0 0.01780253843253885 0.0 17 0.0 0.0 0.0 0.020944162861810414 0.0 18 0.0 0.0 0.0 0.024085787291081975 0.0 19 0.0 0.0 0.0 0.027227411720353537 0.0 20 0.0 0.0 0.0 0.02932182800653458 0.0 21 0.0 0.0 0.0 0.03141624429271562 0.0 22 0.0 0.0 0.0 0.03141624429271562 0.0 23 0.0 0.0 0.0 0.0356050768650777 0.0 24 0.0 0.0 0.0 0.03874670129434926 0.0 25 0.0 0.0 0.0 0.04712436643907343 0.0 26 0.0 0.0 0.0 0.053407615297616555 0.0 27 0.0 0.0 0.0 0.08691827587651321 0.0 28 0.0 0.0 0.0 0.11519289573995728 0.0 29 0.0 0.0 0.0 0.14451472374649185 0.0 30 0.0 0.0 0.0 0.18535584132702215 0.0 31 0.0 0.0 0.0 0.26180203577263017 0.0 32 0.0 0.0 0.0 0.3204456917856993 0.0 33 0.0 0.0 0.0 0.3749005152264064 0.0 34 0.0 0.0 0.0 0.4461106689565618 0.0 35 0.0 0.0 0.0 0.5560675239810665 0.0 36 0.0 0.0 0.0 0.7424705734511792 0.0 37 0.0 0.0 0.0 1.0346416453734344 0.0 38 0.0 0.0 0.0 1.4011644954551166 0.0 39 0.0 0.0 0.0 1.8577472458425837 0.0 40 0.0 0.0 0.0 2.4714112176936287 0.0 41 0.0 0.0 0.0 3.2714782390147867 0.0 42 0.0 0.0 0.0 4.177313282788087 0.0 43 0.0 0.0 0.0 5.0873371591337495 0.0 44 0.0 0.0 0.0 5.87274326645164 0.0 45 0.0 0.0 0.0 6.616261048045909 0.0 46 0.0 0.0 0.0 7.423658526368701 0.0 47 0.0 0.0 0.0 8.199639760398776 0.0 48 0.0 0.0 0.0 8.882419469693795 0.0 49 0.0 0.0 0.0 9.441628618104135 0.0 50 0.0 0.0 0.0 10.101369748251162 0.0 51 0.0 0.0 0.0 10.775771792401457 0.0 52 0.0 0.0 0.0 11.537092112428267 0.0 53 0.0 0.0 0.0 12.424077409625937 0.0 54 0.0 0.0 0.0 13.358187073262682 0.0 55 0.0 0.0 0.0 14.1404515561513 0.0 56 0.0 0.0 0.0 14.849411469023583 0.0 57 0.0 0.0 0.0 15.434800821011184 0.0 58 0.0 0.0 0.0 16.019142964855696 0.0 59 0.0 0.0 0.0 16.598249067984753 0.0 60 0.0 0.0 0.0 17.159552632681272 0.0 61 0.0 0.0 0.0 17.706195283374523 0.0 62 0.0 0.0 0.0 18.29786788422067 0.0 63 0.0 0.0 0.0 18.94818414107988 0.0 64 0.0 0.0 0.0 19.577556235077285 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACAC 15 0.0022194325 69.99371 64 TTAAGTC 15 0.0022194325 69.99371 40 GCGTGTA 15 0.0022194325 69.99371 34 TGGTCGC 15 0.0022194325 69.99371 49 TAGAGAG 20 0.0069350842 52.495285 5 CGGTGGT 20 0.0069350842 52.495285 46 AAGGGAA 20 0.0069350842 52.495285 15 AACTAGC 20 0.0069350842 52.495285 36 TCGGTGG 20 0.0069350842 52.495285 45 GTAGTTC 20 0.0069350842 52.495285 47 TCGCCGT 30 5.8547914E-4 46.662476 52 GTCGCCG 35 0.0012505455 39.99641 51 TGTTAAC 35 0.0012505455 39.99641 18 ATCTCGG 40 0.0024094353 34.996857 42 GGTGGTC 40 0.0024094353 34.996857 47 AAGTCCC 40 0.0024094353 34.996857 42 TCTCGGT 55 2.9746475E-4 31.815323 43 CGGATGA 45 0.0042907726 31.108315 33 CTTTAAC 45 0.0042907726 31.108315 42 ATTGAAC 45 0.0042907726 31.108315 27 >>END_MODULE