FastQCFastQC Report
Wed 25 May 2016
SRR1780418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95492
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT35913.7605244418380597No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT33223.47882545134671RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC11531.2074309889833703No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC5660.5927198089892347No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG4140.4335441712394756No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2950.3089264022117036No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC2010.21048883676119465No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1690.176978176182298No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1430.14975076446194446No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1310.1371842667448582No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT1080.11309847945377624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCA150.002218642570.024
ACTCGCA150.002218642570.021
TACTCGC150.002218642570.020
TTAATAC308.321616E-658.3333322
CTATACA850.057.6470571
TAATTCA252.3799074E-456.00000456
CATTCTG200.006932621452.529
TCCTATA200.006932621452.52
GCTTGAC200.006932621452.556
ATGTAAT200.006932621452.518
GGGCGGA200.006932621452.56
ACAATTC200.006932621452.521
TCCGTAT200.006932621452.513
TCTTTAT608.367351E-1152.51
GACTTGC305.852202E-446.66666423
TTTACAC2050.044.3902442
TTACACA2050.044.3902443
CTTTACA2200.041.3636361
ATAGCGG350.001249993640.07
CTTTATA701.8966603E-840.02
Produced by FastQC (version 0.11.5)