##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95492 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.849987433502285 34.0 34.0 34.0 31.0 34.0 2 33.25690110166297 34.0 34.0 34.0 32.0 34.0 3 33.47574665940602 34.0 34.0 34.0 34.0 34.0 4 33.51292254848574 34.0 34.0 34.0 34.0 34.0 5 33.53399237632472 34.0 34.0 34.0 34.0 34.0 6 37.26659824906798 38.0 37.0 38.0 36.0 38.0 7 36.939000125664975 38.0 37.0 38.0 35.0 38.0 8 36.9176266074645 38.0 37.0 38.0 35.0 38.0 9 36.69623633393373 38.0 37.0 38.0 34.0 38.0 10-11 36.45380764880828 38.0 37.0 38.0 34.0 38.0 12-13 36.31853977296527 38.0 37.0 38.0 34.0 38.0 14-15 36.53055753361538 38.0 37.0 38.0 34.0 38.0 16-17 36.61366397185104 38.0 37.0 38.0 34.0 38.0 18-19 36.66967389938424 38.0 37.0 38.0 34.0 38.0 20-21 36.42705671679303 38.0 37.0 38.0 34.0 38.0 22-23 36.48819796422737 38.0 37.0 38.0 34.0 38.0 24-25 35.76912725673355 38.0 36.5 38.0 31.5 38.0 26-27 36.0023038579148 38.0 37.0 38.0 33.0 38.0 28-29 35.85224940309136 38.0 37.0 38.0 32.0 38.0 30-31 35.55885309764169 38.0 36.0 38.0 31.0 38.0 32-33 36.04741758471914 38.0 37.0 38.0 34.0 38.0 34-35 35.95931072760022 38.0 37.0 38.0 33.0 38.0 36-37 35.97999832446697 38.0 37.0 38.0 33.5 38.0 38-39 35.498371591337495 38.0 36.5 38.0 29.5 38.0 40-41 35.31026682863486 38.0 36.0 38.0 29.0 38.0 42-43 35.83536840782474 38.0 36.5 38.0 32.5 38.0 44-45 36.03269383822729 38.0 37.0 38.0 33.0 38.0 46-47 36.266750094248735 38.0 37.0 38.0 34.0 38.0 48-49 36.02465127968835 38.0 37.0 38.0 33.0 38.0 50-51 36.16020190172999 38.0 37.0 38.0 34.0 38.0 52-53 36.04949629288318 38.0 37.0 38.0 34.0 38.0 54-55 36.26648305617225 38.0 37.0 38.0 34.0 38.0 56-57 36.27193377455703 38.0 37.0 38.0 34.0 38.0 58-59 36.26943618313576 38.0 37.0 38.0 34.0 38.0 60-61 35.99043898965358 38.0 37.0 38.0 32.5 38.0 62-63 36.34413877602312 38.0 37.0 38.0 34.0 38.0 64-65 36.35024923553806 38.0 37.0 38.0 34.0 38.0 66-67 35.55201482846731 38.0 36.5 38.0 31.0 38.0 68-69 34.43692141750094 37.5 34.5 38.0 24.5 38.0 70-71 33.854076781301046 37.0 34.0 38.0 22.0 38.0 72-73 33.132691743811 37.0 34.0 38.0 20.0 38.0 74-75 33.5664139404348 37.5 34.0 38.0 21.0 38.0 76 33.17085200854522 37.0 34.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2401562844846552 1101 2 0.5185023075068003 1101 3 0.38176367726947547 1101 4 0.43936929034817496 1101 5 0.40857855451359626 1101 6 -0.9870071268663239 1101 7 0.32266044919155235 1101 8 0.5088063422271389 1101 9 0.24387231192037007 1101 10-11 -0.07831415270050712 1101 12-13 0.745345763248018 1101 14-15 0.8025724354350956 1101 16-17 0.009973386538590034 1101 18-19 -0.3415597538761972 1101 20-21 0.21709130480628147 1101 22-23 0.6810475107103287 1101 24-25 -1.2901060127636583 1101 26-27 -0.8048498177887211 1101 28-29 0.8817080372531478 1101 30-31 0.8336572764574086 1101 32-33 0.5225778037893107 1101 34-35 0.8316992835781392 1101 36-37 0.5079353199469736 1101 38-39 0.5525898842093042 1101 40-41 1.3371957049815961 1101 42-43 0.5121042310051038 1101 44-45 0.6841585687795089 1101 46-47 0.7755456357681894 1101 48-49 -1.4566708421821914 1101 50-51 -1.1327614424170918 1101 52-53 -1.2460943608765476 1101 54-55 -0.8369495501811954 1101 56-57 -0.4549829699513097 1101 58-59 -0.9915810794738462 1101 60-61 -1.1137566763942743 1101 62-63 -0.6261485769069921 1101 64-65 -1.4512841001186914 1101 66-67 -1.7468458365639137 1101 68-69 -0.6685384663501708 1101 70-71 -0.2537369221749515 1101 72-73 -1.0357095490504165 1101 74-75 -0.3651887083557668 1101 76 -0.7590368123406463 1102 1 -0.45839719455662475 1102 2 -0.17429676334104727 1102 3 0.21842295295314784 1102 4 0.3835145759568803 1102 5 0.02282203461073351 1102 6 0.36248078359719926 1102 7 -0.22084076504136618 1102 8 0.41789725131805255 1102 9 0.3012052229118183 1102 10-11 0.4930616259923468 1102 12-13 0.6558884725712133 1102 14-15 0.05112063520280685 1102 16-17 0.27021517726818445 1102 18-19 -0.00901831157865729 1102 20-21 0.5732574963383357 1102 22-23 0.39110980613848056 1102 24-25 -4.901366703292641 1102 26-27 -1.188626610102098 1102 28-29 0.03950552431893328 1102 30-31 1.5916289795510536 1102 32-33 0.5514818265957757 1102 34-35 0.9787798452928484 1102 36-37 -2.8676849946977327 1102 38-39 -0.06775115686344435 1102 40-41 0.726990869167004 1102 42-43 1.0170667481627405 1102 44-45 0.8747403447271367 1102 46-47 1.0548456040925416 1102 48-49 -3.3391278161025824 1102 50-51 -0.6325766678420734 1102 52-53 -0.24387706597632075 1102 54-55 0.3430472822546591 1102 56-57 0.6628768125426134 1102 58-59 -0.10619410872101298 1102 60-61 -5.701260635849447 1102 62-63 -2.34397140448592 1102 64-65 0.02659574361481276 1102 66-67 1.181831177478955 1102 68-69 -0.4168227024392763 1102 70-71 -1.1600034199048679 1102 72-73 -5.616297660126332 1102 74-75 -2.1165349231166175 1102 76 0.016805231794080555 1103 1 0.6072368924411791 1103 2 0.6361595893976641 1103 3 0.01757433862758262 1103 4 0.40559041715324895 1103 5 0.3288869565738253 1103 6 0.3212369405018336 1103 7 0.8204327498835653 1103 8 0.5407682975467623 1103 9 0.3576113709149027 1103 10-11 0.0858009292977826 1103 12-13 -0.48598738049388146 1103 14-15 -0.35201567718222293 1103 16-17 0.17538400997841563 1103 18-19 0.5657695348399514 1103 20-21 -0.322683397377169 1103 22-23 0.41061903407747735 1103 24-25 -3.077068442061851 1103 26-27 -0.1580349538205681 1103 28-29 0.15133864994945867 1103 30-31 1.1025927205753305 1103 32-33 1.3305453836547017 1103 34-35 0.41790808694022985 1103 36-37 -0.3377609545814195 1103 38-39 0.27279243105418516 1103 40-41 0.3205906555298341 1103 42-43 -0.5848559863930731 1103 44-45 1.0347605215712434 1103 46-47 0.7542402987694103 1103 48-49 -2.260230421289215 1103 50-51 -0.7670790712602695 1103 52-53 0.12202110030461455 1103 54-55 -0.1043445639265741 1103 56-57 0.3982337646522822 1103 58-59 -0.28133208272849686 1103 60-61 -3.953931100510296 1103 62-63 -0.9850609540675421 1103 64-65 0.4263346315146421 1103 66-67 -0.2560805191562636 1103 68-69 0.7233470204258339 1103 70-71 0.8963878921834407 1103 72-73 -3.6273059088949466 1103 74-75 -1.6406729564677498 1103 76 -2.939606850574915 1104 1 0.2665563935619346 1104 2 0.2802195928461373 1104 3 0.22344823942648162 1104 4 -0.31890524351690885 1104 5 -0.25798585220829295 1104 6 0.16979145512911487 1104 7 0.6664583835406717 1104 8 0.46645368176424284 1104 9 0.8765205036236807 1104 10-11 -0.19095348187526895 1104 12-13 0.26682887331380556 1104 14-15 0.4966106806544417 1104 16-17 0.35825371513388404 1104 18-19 0.6841671438979446 1104 20-21 -0.42813486010491886 1104 22-23 -0.07813031933363845 1104 24-25 0.2697330982565589 1104 26-27 0.6411058013952484 1104 28-29 1.106594699147685 1104 30-31 0.673185858111971 1104 32-33 0.26628881718536235 1104 34-35 0.0359464799513205 1104 36-37 0.904192605968035 1104 38-39 1.3156109838445715 1104 40-41 1.5055581823991915 1104 42-43 0.5165866252512714 1104 44-45 0.3582561247668252 1104 46-47 0.618269840697117 1104 48-49 -0.1550807000408696 1104 50-51 0.41567568476018835 1104 52-53 0.5831826613138631 1104 54-55 0.12247881672748662 1104 56-57 -0.19596705148656923 1104 58-59 -0.2749092998099343 1104 60-61 0.25432799484001123 1104 62-63 0.3650741111660878 1104 64-65 0.3848954683443466 1104 66-67 -0.3274351947123293 1104 68-69 0.719180114281798 1104 70-71 0.8790305727972694 1104 72-73 0.9314905736615202 1104 74-75 0.18451814646920184 1104 76 -0.6840461861689136 1105 1 0.8971145223252464 1105 2 0.42939166324909905 1105 3 0.2713581514227741 1105 4 0.015508837075962845 1105 5 -0.12812248954038807 1105 6 0.0791534282648243 1105 7 0.6567895553794827 1105 8 -0.3014654937799861 1105 9 0.6557006796310887 1105 10-11 0.2810966570779527 1105 12-13 -0.11403321561626001 1105 14-15 0.22300310770400245 1105 16-17 0.2547769897980743 1105 18-19 0.6338110762740428 1105 20-21 0.5897119439710465 1105 22-23 0.060750528280991034 1105 24-25 0.2589931723649386 1105 26-27 0.11493627846796528 1105 28-29 1.0096603545437048 1105 30-31 1.2564275209188267 1105 32-33 0.671236130756462 1105 34-35 -0.9010324454771208 1105 36-37 0.3177376499698923 1105 38-39 -0.4456355423249434 1105 40-41 0.3564295348771864 1105 42-43 0.3088081398413678 1105 44-45 0.8476898903990673 1105 46-47 0.5998151162876653 1105 48-49 -0.04710246937852247 1105 50-51 0.47847964492693507 1105 52-53 0.039166334310621664 1105 54-55 0.507277913531567 1105 56-57 0.0820841296157866 1105 58-59 0.11614269185287185 1105 60-61 0.6827960355996225 1105 62-63 -0.5174380472405815 1105 64-65 0.5974649449538845 1105 66-67 0.6063738604057818 1105 68-69 0.22663437551385357 1105 70-71 0.3017523189760496 1105 72-73 0.314581703814369 1105 74-75 -0.40736466964936824 1105 76 0.20227271146887915 1106 1 -1.217794411959762 1106 2 -0.18482504854021897 1106 3 0.2519297733770003 1106 4 0.155430077538675 1106 5 -0.1203388321443768 1106 6 0.35965736624168443 1106 7 -0.7190718770595979 1106 8 0.4611329556416237 1106 9 -0.43985679021877644 1106 10-11 0.49629324826673127 1106 12-13 1.0297106312948898 1106 14-15 -0.19473778572449874 1106 16-17 0.28588854529893126 1106 18-19 0.3978939583808625 1106 20-21 0.23799369390951597 1106 22-23 0.3839492115199832 1106 24-25 -0.5157576275366083 1106 26-27 0.7356598817167921 1106 28-29 0.7114278293160439 1106 30-31 0.5565828864123006 1106 32-33 0.06354938450984804 1106 34-35 0.5647409977914037 1106 36-37 1.0792823829258396 1106 38-39 0.48945706166225733 1106 40-41 1.0479674994353942 1106 42-43 0.9197098750124226 1106 44-45 1.1730190806525655 1106 46-47 0.883680179049172 1106 48-49 1.265874395007394 1106 50-51 0.24279326786565036 1106 52-53 0.6643278528929457 1106 54-55 0.28822025908903015 1106 56-57 0.35378915053629356 1106 58-59 0.7625169842476538 1106 60-61 0.9490863383315897 1106 62-63 0.8293115835740892 1106 64-65 -0.8139151928753066 1106 66-67 0.07355482274887493 1106 68-69 1.4802416238525211 1106 70-71 0.31808107851333745 1106 72-73 0.15882395092241808 1106 74-75 -0.38629034033606047 1106 76 0.3421785692094659 1107 1 0.8032822132822304 1107 2 -0.15011887230840415 1107 3 0.07760266415323969 1107 4 0.10220769572389798 1107 5 -0.1484253868187011 1107 6 0.16623639578018867 1107 7 0.5199370531845773 1107 8 0.01739973888023627 1107 9 -0.7049226741880474 1107 10-11 0.40991030940455175 1107 12-13 0.1092163454021815 1107 14-15 0.2532653991877538 1107 16-17 0.5215954709218664 1107 18-19 0.5304034818930106 1107 20-21 0.5467740598621802 1107 22-23 0.015123882100141373 1107 24-25 0.23534304067047174 1107 26-27 -0.0936897092405573 1107 28-29 0.7796614519976046 1107 30-31 0.47210464997940704 1107 32-33 0.7764545240839382 1107 34-35 -0.06625413116631762 1107 36-37 0.9901750950972001 1107 38-39 0.2271311389744426 1107 40-41 0.13866724339343506 1107 42-43 0.5447332934849101 1107 44-45 0.6050977042708823 1107 46-47 0.5545725789742377 1107 48-49 0.8047412531851279 1107 50-51 0.69887582578734 1107 52-53 0.543254350113962 1107 54-55 0.46686417340864494 1107 56-57 0.7304050251381753 1107 58-59 0.6061284249520824 1107 60-61 0.887745552719899 1107 62-63 0.5245670010473944 1107 64-65 0.5667911082550248 1107 66-67 -0.3073388261613843 1107 68-69 0.7874607583057696 1107 70-71 0.20899002749229822 1107 72-73 0.8450086133841666 1107 74-75 -0.031047724961055678 1107 76 0.7685040547524622 1108 1 -0.7695257897135477 1108 2 0.36950077140848947 1108 3 -0.13143280366893606 1108 4 0.20220502092584525 1108 5 -0.094889303792975 1108 6 -0.20164195440437283 1108 7 0.03289644975013317 1108 8 -0.6286977307608055 1108 9 -0.15565289405645188 1108 10-11 0.23525937055043755 1108 12-13 0.7051279165785616 1108 14-15 0.5332431983639196 1108 16-17 -0.1310218189727408 1108 18-19 0.17591918295757836 1108 20-21 1.001944444498136 1108 22-23 0.1361049980258855 1108 24-25 1.0644301320950689 1108 26-27 -0.2955634052765035 1108 28-29 -1.1081909526458418 1108 30-31 0.14252940791018176 1108 32-33 0.8371055699299816 1108 34-35 -0.06370638602108158 1108 36-37 0.8925650271573318 1108 38-39 0.8248749468169407 1108 40-41 0.5334206270129656 1108 42-43 -0.5398438209429486 1108 44-45 0.6358645688726057 1108 46-47 0.8582156539220733 1108 48-49 0.7337587998603468 1108 50-51 0.8351434125845358 1108 52-53 -0.2763973911795716 1108 54-55 -0.2115247957241806 1108 56-57 0.07110742710683127 1108 58-59 0.28754660287964384 1108 60-61 0.759422116139362 1108 62-63 0.8653563339527537 1108 64-65 0.3802105700856586 1108 66-67 0.49313461309395734 1108 68-69 1.4796717463761695 1108 70-71 -0.6247512125440835 1108 72-73 -0.31721120151980386 1108 74-75 -0.6062630462042904 1108 76 0.3848443960566925 1109 1 -0.9040328597044862 1109 2 0.31337410237859586 1109 3 0.2905215860273884 1109 4 0.1123629633418517 1109 5 0.03162217755721741 1109 6 -0.3652955051546982 1109 7 -0.33068620415509997 1109 8 0.34097417439497235 1109 9 0.5262565943046553 1109 10-11 0.4907834163970648 1109 12-13 0.35223887014112165 1109 14-15 0.558982691845344 1109 16-17 0.6929570695222793 1109 18-19 0.6747572624408136 1109 20-21 1.0457626334776151 1109 22-23 0.4213072509700737 1109 24-25 -0.3052575279151668 1109 26-27 -0.057763570702469735 1109 28-29 -0.6161274605823479 1109 30-31 0.524466467266592 1109 32-33 0.7218785030900179 1109 34-35 -0.2327362808574236 1109 36-37 0.8917182037298517 1109 38-39 1.002806334425749 1109 40-41 0.6287374965233923 1109 42-43 0.6579583768592485 1109 44-45 0.6448645294854671 1109 46-47 0.7524021126246581 1109 48-49 0.5867857012743443 1109 50-51 -0.12460293425858993 1109 52-53 -0.30386991865210433 1109 54-55 -0.358261571493216 1109 56-57 0.0017269867586477972 1109 58-59 -0.07416516205792334 1109 60-61 0.5555739929364023 1109 62-63 0.06382811306969671 1109 64-65 0.6965680477334573 1109 66-67 0.4885853988673148 1109 68-69 -0.38398968180138127 1109 70-71 0.23660606816803664 1109 72-73 0.2942937809529198 1109 74-75 -0.051002894169954516 1109 76 0.5120254587216237 1110 1 -0.5824042966966161 1110 2 -0.6955097340914449 1110 3 -0.21733071492835876 1110 4 0.1463404800779884 1110 5 0.42086894611433223 1110 6 -0.12896861365539536 1110 7 0.5088573940091905 1110 8 0.5481654505517639 1110 9 0.7887297533295339 1110 10-11 -0.8479812671952018 1110 12-13 0.1839933225852377 1110 14-15 0.5105636779090901 1110 16-17 0.24767055527024695 1110 18-19 -0.3296425523038238 1110 20-21 0.7888681869279921 1110 22-23 -0.7658848672565313 1110 24-25 -1.0343215673240351 1110 26-27 0.7705594472757156 1110 28-29 -0.08328673808644993 1110 30-31 -0.2930780571744833 1110 32-33 0.8165271494628001 1110 34-35 0.580218963132296 1110 36-37 0.9588732708849221 1110 38-39 0.06520370716796009 1110 40-41 -1.3111597709600886 1110 42-43 -0.1643648937398865 1110 44-45 0.6775157862746539 1110 46-47 0.3154520894677404 1110 48-49 0.9564753985502534 1110 50-51 -0.6151717524136089 1110 52-53 -0.174864824129763 1110 54-55 -0.700710310493676 1110 56-57 0.19822397635908118 1110 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36-37 0.5759042362765427 2116 38-39 -3.6214130254493426 2116 40-41 -1.004316607842192 2116 42-43 -1.1826033837844747 2116 44-45 -0.2510926554061399 2116 46-47 -0.34639390010577387 2116 48-49 0.313794348364965 2116 50-51 -0.5175726694414351 2116 52-53 0.31240042302807325 2116 54-55 -0.6272146129872453 2116 56-57 0.060567736173162245 2116 58-59 -1.7743768356302496 2116 60-61 -1.241444562278872 2116 62-63 0.34240404533415614 2116 64-65 -1.4785355372061915 2116 66-67 -0.4720072871351988 2116 68-69 -0.9532414682662846 2116 70-71 1.0639569959287982 2116 72-73 0.523929582310025 2116 74-75 0.4367770854133113 2116 76 0.10083828523936944 2117 1 0.7822728025047923 2117 2 0.5437461399795538 2117 3 0.23791555751774496 2117 4 0.19564113095736957 2117 5 0.22514351565686752 2117 6 0.347004243289625 2117 7 0.654220455450357 2117 8 0.7833590987076064 2117 9 -0.606229393603023 2117 10-11 -0.16317306888472416 2117 12-13 -1.1089023982290627 2117 14-15 0.2145058835131195 2117 16-17 -0.6260773097530716 2117 18-19 -0.8063539957788706 2117 20-21 0.25620881380331895 2117 22-23 0.5991615252098867 2117 24-25 -1.44008254225826 2117 26-27 -1.7068213402421648 2117 28-29 -1.2219794399283046 2117 30-31 0.1338711188399202 2117 32-33 -1.6519905996947486 2117 34-35 0.6582783270246466 2117 36-37 -0.561876394443054 2117 38-39 0.3745791399334948 2117 40-41 0.31899302412831076 2117 42-43 0.4541320826715065 2117 44-45 -0.150139663109087 2117 46-47 -0.3543766507806012 2117 48-49 -0.09000939118339346 2117 50-51 0.023682515231683965 2117 52-53 0.8019158618324767 2117 54-55 -0.06813402835362581 2117 56-57 0.401612242065589 2117 58-59 -0.7334837551114504 2117 60-61 -0.23556859941101038 2117 62-63 -0.08679636285598491 2117 64-65 -0.8313237391944739 2117 66-67 -0.43943437252595174 2117 68-69 0.10457998146707581 2117 70-71 0.3280708283074958 2117 72-73 1.271962698380868 2117 74-75 1.2006851767952327 2117 76 1.3511180929949802 2118 1 0.2190354782076014 2118 2 0.49487879560204107 2118 3 -0.4931179515183999 2118 4 0.3551228076641948 2118 5 0.3806091281066699 2118 6 0.23287585473541128 2118 7 0.7595009524383087 2118 8 0.5572201679847169 2118 9 -1.0536283644865563 2118 10-11 -0.6755899238044023 2118 12-13 -0.80808192885155 2118 14-15 0.6895702032922344 2118 16-17 -2.4443268704960417 2118 18-19 -0.3519426001965442 2118 20-21 -0.5588404457348375 2118 22-23 0.7388295723986431 2118 24-25 1.2009738708943516 2118 26-27 -1.3797787950614477 2118 28-29 0.06802432513193679 2118 30-31 -0.28183075113999934 2118 32-33 0.5024631528152881 2118 34-35 0.0946017456993502 2118 36-37 -2.6730711735595207 2118 38-39 -0.24365943938376944 2118 40-41 0.009003064288954477 2118 42-43 1.0057290957237157 2118 44-45 -2.1642586390127008 2118 46-47 0.25801824128766526 2118 48-49 0.6235087438567675 2118 50-51 -0.3349394727201229 2118 52-53 -0.6976213495129073 2118 54-55 -0.564298373233143 2118 56-57 0.3828653796157866 2118 58-59 -0.44337017579418614 2118 60-61 -0.4804622364592035 2118 62-63 -0.7966200325346975 2118 64-65 0.18776825377741346 2118 66-67 0.6805926104057818 2118 68-69 -0.7002957715449725 2118 70-71 0.30198209838781764 2118 72-73 0.897072512637898 2118 74-75 0.5102594112329797 2118 76 0.44446021146887915 2119 1 0.8314563207186154 2119 2 -0.8953848332525851 2119 3 -0.05649556715716386 2119 4 -0.28780808440628647 2119 5 -0.27113233665104275 2119 6 -0.6034230164099625 2119 7 -0.9901720927158735 2119 8 0.28280180344727057 2119 9 -0.07019981272443943 2119 10-11 -0.2651669474828182 2119 12-13 -0.3261590901951621 2119 14-15 -0.7395924481394829 2119 16-17 -0.7916647697251165 2119 18-19 -0.8508756949102008 2119 20-21 -1.5582295564176576 2119 22-23 -0.0645329000683148 2119 24-25 0.7515916434714498 2119 26-27 0.22778934921168315 2119 28-29 -0.3634528224451543 2119 30-31 0.6711479795868698 2119 32-33 0.29836589235273436 2119 34-35 0.5592389979019927 2119 36-37 -0.0296240369956422 2119 38-39 -1.9417080351987508 2119 40-41 -1.259444072274924 2119 42-43 -0.188155127844567 2119 44-45 -0.1866292337263573 2119 46-47 -0.3144194652101291 2119 48-49 -0.6410063304824405 2119 50-51 0.717992796002342 2119 52-53 -0.08990693596665267 2119 54-55 0.06521876608403687 2119 56-57 -1.6439621258908161 2119 58-59 -0.32688820993515577 2119 60-61 -0.13472469515964747 2119 62-63 -0.6687806878210409 2119 64-65 -0.5874761019494557 2119 66-67 0.1153220982912444 2119 68-69 0.6209803848426958 2119 70-71 -0.1637908917002875 2119 72-73 0.4676087185850335 2119 74-75 0.42565554284090723 2119 76 0.28019121367152167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 4.0 22 14.0 23 24.0 24 54.0 25 106.0 26 210.0 27 413.0 28 664.0 29 1152.0 30 1797.0 31 2831.0 32 4176.0 33 6257.0 34 9612.0 35 13036.0 36 19403.0 37 35737.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.243957609014366 20.55564864072383 10.628115444225694 31.572278306036104 2 19.320990240020105 18.781678046328487 39.310099275331964 22.58723243831944 3 16.81083232103213 24.97905583713819 34.09290830645499 24.11720353537469 4 12.288987559167259 21.141037992711432 35.38830477945796 31.181669668663343 5 11.612491098730784 37.26594898001927 36.582122062581156 14.539437858668789 6 23.59569388011561 38.7100490093411 17.953336405143887 19.740920705399407 7 24.26171825912118 33.79654840196037 20.092782641477818 21.84895069744062 8 21.514891299794748 35.471034222762114 21.749465923847026 21.264608553596112 9 20.83211159049973 19.607925271226907 19.096887697398735 40.46307544087463 10-11 21.002806517823483 25.118334520169228 29.531269635152686 24.347589326854603 12-13 23.352741590918612 20.56140828551083 27.665144724165376 28.42070539940519 14-15 22.80819335651154 23.530766975244 21.741611862773844 31.91942780547061 16-17 24.085263687010432 27.5578058894986 22.170443597369413 26.18648682612156 18-19 22.167825577011687 26.76088049260671 23.33860428098689 27.732689649394715 20-21 21.198634440581408 25.107338834666777 23.143823566372053 30.55020315837976 22-23 23.225505801533114 28.876764545721105 23.317136514053534 24.580593138692247 24-25 23.61140200226197 30.234469903237965 23.340698697273073 22.81342939722699 26-27 21.866753235873162 28.6568508356721 28.46311732920035 21.013278599254388 28-29 21.810203996146274 26.373413479663217 29.506136639718513 22.310245884471996 30-31 22.851128890378252 24.331357600636704 27.937942445440456 24.87957106354459 32-33 19.179093536631342 26.933669836216644 26.28492439157207 27.602312235579944 34-35 18.93247601893352 26.01160306622544 27.72012315167763 27.335797763163406 36-37 19.17019226741507 26.937858668789012 30.481087420935786 23.410861642860134 38-39 21.00961337075357 24.585829179407696 28.614962509948477 25.789594939890254 40-41 20.09592426590709 24.880094667616135 30.340761529761657 24.683219536715118 42-43 23.833933732668704 23.90828551082813 31.225652410673142 21.032128345830017 44-45 19.836740250492188 25.318351275499516 29.73495161898379 25.109956855024507 46-47 23.777908097013363 22.046349432413187 28.197650064926904 25.978092405646546 48-49 21.486616679931302 22.10865831692707 29.82867674779039 26.576048255351232 50-51 18.05020315837976 22.469945126293304 32.52209609181921 26.95775562350773 52-53 18.99478490344741 22.47046873036485 35.51972940141582 23.015016964771917 54-55 17.7140493444477 22.83803878858962 32.89071335818707 26.557198508775603 56-57 21.646315921752606 22.653206551334144 30.067963808486574 25.632513718426676 58-59 18.00936204079923 27.145205881120933 32.06603694550329 22.779395132576553 60-61 19.462363339337326 32.511100406316764 25.969191136430275 22.057345117915634 62-63 16.21287647132744 37.22615507058183 24.223495161898377 22.33747329619235 64-65 16.576781301051398 38.03145813261844 22.8678842206677 22.523876345662465 66-67 16.384618606794284 37.60838604280987 22.085096133707534 23.92189921668831 68-69 16.034851087002053 36.25434591379383 22.821283458300172 24.88951954090395 70-71 17.06006785908767 34.09238470238344 23.15324843965987 25.694298998869016 72-73 17.21610187240816 32.252963599044946 23.415050475432498 27.115884053114396 74-75 17.482092740753153 31.54662170653039 23.698320278138482 27.272965274577977 76 17.45277091274662 31.927281866543794 23.376827378209693 27.243119842499897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.5 15 2.5 16 7.0 17 10.0 18 11.0 19 14.5 20 21.0 21 25.0 22 30.0 23 46.0 24 68.0 25 79.0 26 147.0 27 245.0 28 353.0 29 431.0 30 514.0 31 673.0 32 948.0 33 1147.0 34 1341.5 35 1796.0 36 2196.0 37 2336.0 38 2739.0 39 3779.0 40 5707.0 41 8024.0 42 9050.0 43 8874.0 44 8444.5 45 7790.0 46 7389.0 47 7111.5 48 6390.0 49 5673.0 50 5400.0 51 4972.5 52 4033.5 53 3222.0 54 2922.0 55 2710.0 56 2098.5 57 1588.5 58 1478.0 59 1179.0 60 778.0 61 564.0 62 452.0 63 387.0 64 288.5 65 228.0 66 178.0 67 155.0 68 126.5 69 78.5 70 49.5 71 40.0 72 35.0 73 19.5 74 4.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95492.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.51769781761823 #Duplication Level Percentage of deduplicated Percentage of total 1 63.06248614566143 26.81271729568969 2 17.075934090293345 14.520588112093161 3 7.556464126499347 9.638503749005151 4 3.7733060762050195 6.41729150085871 5 2.2561020664515654 4.796213295354584 6 1.4704071328292407 3.751099568550245 7 1.0935691239132042 3.254722908725338 8 0.7265830890864757 2.4714112176936287 9 0.6674712445506268 2.55414066099778 >10 2.261028053496219 14.254597243748169 >50 0.02955592226792443 0.959242659070917 >100 0.017240954656289255 1.5299710970552507 >500 0.0024629935223270364 0.5927198089892347 >1k 0.007388980566981108 8.44678088216814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 3591 3.7605244418380597 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 3322 3.47882545134671 RNA PCR Primer, Index 27 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 1153 1.2074309889833703 No Hit CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 566 0.5927198089892347 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 414 0.4335441712394756 No Hit TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 295 0.3089264022117036 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 201 0.21048883676119465 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 169 0.176978176182298 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT 143 0.14975076446194446 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 131 0.1371842667448582 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT 108 0.11309847945377624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0020944162861810415 0.0 5 0.0 0.0 0.0 0.008377665144724166 0.0 6 0.0 0.0 0.0 0.010472081430905207 0.0 7 0.0 0.0 0.0 0.010472081430905207 0.0 8 0.0 0.0 0.0 0.010472081430905207 0.0 9 0.0 0.0 0.0 0.012566497717086247 0.0 10 0.0 0.0 0.0 0.012566497717086247 0.0 11 0.0 0.0 0.0 0.01466091400326729 0.0 12 0.0 0.0 0.0 0.01466091400326729 0.0 13 0.0 0.0 0.0 0.016755330289448332 0.0 14 0.0 0.0 0.0 0.01884974657562937 0.0 15 0.0 0.0 0.0 0.01884974657562937 0.0 16 0.0 0.0 0.0 0.019896954718719894 0.0 17 0.0 0.0 0.0 0.023038579147991456 0.0 18 0.0 0.0 0.0 0.026180203577263018 0.0 19 0.0 0.0 0.0 0.028274619863444057 0.0 20 0.0 0.0 0.0 0.0303690361496251 0.0 21 0.0 0.0 0.0 0.034557868721987184 0.0 22 0.0 0.0 0.0 0.034557868721987184 0.0 23 0.0 0.0 0.0 0.03874670129434926 0.0 24 0.0 0.0 0.0 0.03874670129434926 0.0 25 0.0 0.0 0.0 0.04607715829598291 0.0 26 0.0 0.0 0.0 0.054454823440707074 0.0 27 0.0 0.0 0.0 0.08796548401960373 0.0 28 0.0 0.0 0.0 0.1162401038830478 0.0 29 0.0 0.0 0.0 0.1424203074603108 0.0 30 0.0 0.0 0.0 0.18849746575629373 0.0 31 0.0 0.0 0.0 0.26703807648808275 0.0 32 0.0 0.0 0.0 0.32882335693042347 0.0 33 0.0 0.0 0.0 0.3853725966573116 0.0 34 0.0 0.0 0.0 0.45658275038746704 0.0 35 0.0 0.0 0.0 0.5728228542705148 0.0 36 0.0 0.0 0.0 0.7581786955975369 0.0 37 0.0 0.0 0.0 1.0587274326645164 0.0 38 0.0 0.0 0.0 1.4388639886063754 0.0 39 0.0 0.0 0.0 1.9101076529971097 0.0 40 0.0 0.0 0.0 2.55414066099778 0.0 41 0.0 0.0 0.0 3.395048799899468 0.0 42 0.0 0.0 0.0 4.32496963096385 0.0 43 0.0 0.0 0.0 5.29049553889331 0.0 44 0.0 0.0 0.0 6.126167637079546 0.0 45 0.0 0.0 0.0 6.910526536254346 0.0 46 0.0 0.0 0.0 7.7461986344405815 0.0 47 0.0 0.0 0.0 8.554643320906463 0.0 48 0.0 0.0 0.0 9.258367193063293 0.0 49 0.0 0.0 0.0 9.845850961337076 0.0 50 0.0 0.0 0.0 10.554810874209357 0.0 51 0.0 0.0 0.0 11.26377078708164 0.0 52 0.0 0.0 0.0 12.062790600259708 0.0 53 0.0 0.0 0.0 12.993758639467181 0.0 54 0.0 0.0 0.0 13.972898253256817 0.0 55 0.0 0.0 0.0 14.805428727013782 0.0 56 0.0 0.0 0.0 15.540568843463326 0.0 57 0.0 0.0 0.0 16.170988145603822 0.0 58 0.0 0.0 0.0 16.779416076739412 0.0 59 0.0 0.0 0.0 17.387844007875007 0.0 60 0.0 0.0 0.0 17.98265823315042 0.0 61 0.0 0.0 0.0 18.574330833996566 0.0 62 0.0 0.0 0.0 19.181711556989068 0.0 63 0.0 0.0 0.0 19.859255225568635 0.0 64 0.0 0.0 0.0 20.49700498471076 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCA 15 0.0022186425 70.0 24 ACTCGCA 15 0.0022186425 70.0 21 TACTCGC 15 0.0022186425 70.0 20 TTAATAC 30 8.321616E-6 58.333332 2 CTATACA 85 0.0 57.647057 1 TAATTCA 25 2.3799074E-4 56.000004 56 CATTCTG 20 0.0069326214 52.5 29 TCCTATA 20 0.0069326214 52.5 2 GCTTGAC 20 0.0069326214 52.5 56 ATGTAAT 20 0.0069326214 52.5 18 GGGCGGA 20 0.0069326214 52.5 6 ACAATTC 20 0.0069326214 52.5 21 TCCGTAT 20 0.0069326214 52.5 13 TCTTTAT 60 8.367351E-11 52.5 1 GACTTGC 30 5.852202E-4 46.666664 23 TTTACAC 205 0.0 44.390244 2 TTACACA 205 0.0 44.390244 3 CTTTACA 220 0.0 41.363636 1 ATAGCGG 35 0.0012499936 40.0 7 CTTTATA 70 1.8966603E-8 40.0 2 >>END_MODULE