FastQCFastQC Report
Wed 25 May 2016
SRR1780417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760544
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC300.005737560329.3397436
TCTTTCG406.980316E-427.53319220
GATATTA407.0261466E-427.5023861
TAAACGG508.354919E-526.39186926
TTTTCGA450.001389013124.47072421
GATCATA450.001397029724.4465661
TCTTCGC551.58979E-424.00682334
ACCGAAT651.8082595E-523.69904134
CGGAATC752.0410644E-623.4949920
CGGTGGT1355.456968E-1222.79878843
GCGGAAT803.5811408E-622.02220219
CGTGCGA904.0691157E-722.00480710
CCGAATT703.207913E-522.00480535
CCGTGCG956.918981E-720.8233579
TCGGTGG2650.020.741542
TGATTCG856.1457777E-620.69133843
CGAATTG755.4600183E-520.53781936
GAACCGA755.4933145E-520.52160832
CGACAAT654.8964267E-420.32818417
GTAAACG654.9335265E-420.30544525
Produced by FastQC (version 0.11.5)