FastQCFastQC Report
Wed 25 May 2016
SRR1780417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760544
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT354034.6549575041023274No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT284783.7444250431270247Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC88251.1603536416039046No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC47680.6269196785458829No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG45280.5953633188875331No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT28640.3765725585896411No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC22280.2929482054950141No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC15350.201829216981529No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG11940.15699288930029032No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9020.11859931838263138No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC8360.10992131947658518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT30700.042.6341744
GCCGTCT33300.039.30537443
CTTATAA1000.035.2312241
ACCGTCA250.002353401435.19647620
CTATACA7500.034.350441
TTTACAC11250.033.0477522
CTTTACA12150.030.6279641
TTACACA12450.029.8624273
GATCGCC300.005733586429.343911
CTTTATA2650.028.2254452
TTATACA59750.027.8350262
GCAGCGT406.993379E-427.5243911
CGAGATC407.0149306E-427.50990915
CGGATTC407.033833E-427.49724833
CTTATAC61000.027.3619521
ATTACAC1250.026.3990924
CGGCATT508.3431136E-526.39735842
TGCCGTC49750.026.26470842
TATACAC66150.025.4080453
TCTTTAT3000.024.9554481
Produced by FastQC (version 0.11.5)