FastQCFastQC Report
Wed 25 May 2016
SRR1780416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50404
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACC150.002178370770.27491
ACGATGC150.002213045569.9960424
GCCTGAG150.002213045569.9960430
GCGGGGT150.002213045569.9960426
CGATGCA150.002213045569.9960425
GGCCTGA150.002213045569.9960429
GGGGACT200.006915274552.49702549
CGGGGAC200.006915274552.49702548
CCGTAAC200.006915274552.49702555
GCGGGGA200.006915274552.49702547
CGCAACA200.006915274552.49702557
CCTGAGG200.006915274552.49702531
GACTCAG200.006915274552.49702510
CAGGAGT450.004271046731.1093541
GCAGGAG450.004271046731.1093540
GAGGCCT450.004271046731.109359
CCCTCGT500.00714814227.99841352
ACCCTCG500.00714814227.99841351
GATCTCG657.8562705E-426.9215541
CTTTACA800.00257289821.9609071
Produced by FastQC (version 0.11.5)