##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50404 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.215597968415207 32.0 21.0 34.0 12.0 34.0 2 26.983850488056504 34.0 21.0 34.0 11.0 34.0 3 27.386794698833427 34.0 21.0 34.0 11.0 34.0 4 27.415959050868977 34.0 21.0 34.0 11.0 34.0 5 27.879096897071662 34.0 23.0 34.0 11.0 34.0 6 30.402408539004842 37.0 26.0 38.0 11.0 38.0 7 29.907904134592492 36.0 24.0 38.0 11.0 38.0 8 29.59527021664947 34.0 23.0 38.0 11.0 38.0 9 29.849476232045077 36.0 23.0 38.0 12.0 38.0 10-11 29.717304182207762 36.0 22.5 38.0 11.0 38.0 12-13 29.92704944052059 36.0 23.0 38.0 11.0 38.0 14-15 29.548041822077614 36.0 21.5 38.0 11.0 38.0 16-17 29.294678993730656 36.0 21.0 38.0 11.0 38.0 18-19 29.393222760098404 35.5 21.5 38.0 11.0 38.0 20-21 29.527656535195618 36.0 22.0 38.0 11.0 38.0 22-23 29.270653122768035 36.0 21.0 38.0 11.0 38.0 24-25 29.603652487897786 36.0 21.5 38.0 11.0 38.0 26-27 29.56681017379573 36.0 21.0 38.0 11.0 38.0 28-29 29.439915482898183 36.0 21.0 38.0 11.0 38.0 30-31 29.345081739544483 36.0 21.0 38.0 11.0 38.0 32-33 29.270633283072772 36.0 21.0 38.0 11.0 38.0 34-35 29.29054241726847 36.0 21.0 38.0 11.0 38.0 36-37 29.23895920958654 35.5 21.0 38.0 11.0 38.0 38-39 29.353037457344655 36.0 21.0 38.0 11.0 38.0 40-41 29.14998809618284 36.0 21.0 38.0 11.0 38.0 42-43 29.456134433775098 36.0 21.0 38.0 11.0 38.0 44-45 28.775176573287833 34.0 21.0 38.0 11.0 38.0 46-47 29.296038012856123 36.0 21.0 38.0 11.0 38.0 48-49 29.227610903896515 35.0 21.0 38.0 11.0 38.0 50-51 29.215617808110466 35.0 21.0 38.0 11.0 38.0 52-53 29.155493611618127 35.0 21.0 38.0 11.0 38.0 54-55 29.001150702325212 34.0 21.0 38.0 11.0 38.0 56-57 28.824329418300135 34.5 21.0 38.0 11.0 38.0 58-59 28.75635862233156 34.5 21.0 38.0 11.0 38.0 60-61 28.879989683358463 34.0 21.0 38.0 11.0 38.0 62-63 28.763352114911516 34.0 20.5 38.0 11.0 38.0 64-65 28.878878660423776 34.5 21.0 38.0 11.0 38.0 66-67 29.017687088326323 34.5 21.0 38.0 11.0 38.0 68-69 28.743175144829777 34.0 20.5 38.0 11.0 38.0 70-71 28.760753114832156 34.0 21.0 38.0 11.0 38.0 72-73 28.680531307039125 34.0 20.5 38.0 11.0 38.0 74-75 28.28200142845806 34.0 19.5 37.5 11.0 38.0 76 27.414014760733274 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.449888346888173 1101 2 -0.27486579628613583 1101 3 -0.7878753117315789 1101 4 -1.324861959888107 1101 5 -1.4873152149650828 1101 6 -3.2178838142539803 1101 7 -1.5768488554710984 1101 8 -2.4027454564692867 1101 9 -1.5841704428052594 1101 10-11 -2.180061271124238 1101 12-13 -1.1500783453363965 1101 14-15 -0.8549599951117912 1101 16-17 -1.0697375878040454 1101 18-19 -1.6562310824678548 1101 20-21 -2.1088225864096586 1101 22-23 -0.41358946782251493 1101 24-25 -2.729276921475556 1101 26-27 -1.383590040154516 1101 28-29 -1.498412894553823 1101 30-31 -0.9052617315879736 1101 32-33 -0.7532799819852798 1101 34-35 -0.6927843280719159 1101 36-37 -1.3794510660148873 1101 38-39 -2.2441861317209266 1101 40-41 -1.3769332682129836 1101 42-43 -0.8529988181962693 1101 44-45 -0.6900948177590749 1101 46-47 -1.3558601121991138 1101 48-49 -1.6200248114732752 1101 50-51 -1.9148164710992077 1101 52-53 -1.526810652317863 1101 54-55 -1.573815080039541 1101 56-57 -1.5157532203974746 1101 58-59 -0.9587153582930306 1101 60-61 -1.2902131984933014 1101 62-63 -0.5602678433337047 1101 64-65 -0.31840105557706977 1101 66-67 -1.3148982941445801 1101 68-69 -1.6268981193605896 1101 70-71 -0.4963923626230873 1101 72-73 -1.2903695854722237 1101 74-75 -0.6781826818848273 1101 76 -2.201876478408316 1102 1 -0.5225275720697695 1102 2 -0.801903730103728 1102 3 -0.7350100978494147 1102 4 -0.6206650268776173 1102 5 -0.824011475390158 1102 6 0.06231527102984913 1102 7 0.5925081528689589 1102 8 0.6923581216851389 1102 9 -0.44090974869710564 1102 10-11 -0.9205778167255296 1102 12-13 -3.6885502312659106 1102 14-15 -1.6780027715443957 1102 16-17 -1.1982686612503706 1102 18-19 -0.7102396915612097 1102 20-21 -0.6413084621158731 1102 22-23 -0.42125694159168603 1102 24-25 -1.2914238141309262 1102 26-27 -3.6009696473646287 1102 28-29 -1.645238291379222 1102 30-31 -1.3896577498822928 1102 32-33 -5.226578340876859 1102 34-35 -1.0411163162344153 1102 36-37 -0.21271714080639015 1102 38-39 -0.5428140574674956 1102 40-41 -0.6124054231766642 1102 42-43 -0.7216428859928783 1102 44-45 -5.028607058813687 1102 46-47 -1.7734011399096339 1102 48-49 -0.6289029389954699 1102 50-51 -4.960754055172384 1102 52-53 -1.8608838296113746 1102 54-55 -1.1373071435316042 1102 56-57 -1.4193044714063525 1102 58-59 -0.480910677098521 1102 60-61 -0.6833393755177823 1102 62-63 -3.019374649898136 1102 64-65 -0.9414841871347761 1102 66-67 -2.113324444265647 1102 68-69 -6.508052275938475 1102 70-71 -1.3070595673580847 1102 72-73 -1.5040499728814218 1102 74-75 -4.923407325414551 1102 76 -2.6670365537378835 1103 1 0.1985608633043796 1103 2 -0.2882982879268603 1103 3 -0.4867107781215232 1103 4 0.028994837067962465 1103 5 0.11022888742163417 1103 6 -0.3413128468839801 1103 7 0.41110225717735105 1103 8 1.0823807974221005 1103 9 0.7189541968811248 1103 10-11 -0.043026796317366944 1103 12-13 -1.0009085079099016 1103 14-15 0.3270992692719332 1103 16-17 -0.41935709662452325 1103 18-19 -0.47667960085826167 1103 20-21 -0.5494717274219951 1103 22-23 -0.1865630640406657 1103 24-25 0.21878026750172808 1103 26-27 0.08383760887119607 1103 28-29 -0.10555575169668074 1103 30-31 -0.4860296319684636 1103 32-33 -3.0202291345276535 1103 34-35 -1.1641321892502852 1103 36-37 -0.3380005875184082 1103 38-39 -0.04791609828382093 1103 40-41 0.4012000190002105 1103 42-43 0.47563602557174534 1103 44-45 -3.7746388048454342 1103 46-47 -0.48825374761938534 1103 48-49 -0.020059401580500236 1103 50-51 -0.34794226378916804 1103 52-53 -0.4895686368676131 1103 54-55 -0.17415521609396123 1103 56-57 0.27287239934194574 1103 58-59 0.26228660181304164 1103 60-61 0.7565699215343749 1103 62-63 -0.11517963856026725 1103 64-65 0.3952505067427765 1103 66-67 1.2166075285234683 1103 68-69 -1.3907053371629665 1103 70-71 0.7496297750455412 1103 72-73 1.1098956053498696 1103 74-75 -1.6484640147569571 1103 76 -0.22372589614151295 1104 1 -1.3366032023218715 1104 2 -0.531595606854431 1104 3 -0.07289525191103863 1104 4 -0.160230853208148 1104 5 -0.4299871990129418 1104 6 -0.46651292691599267 1104 7 0.4434652023554193 1104 8 1.7495476641688015 1104 9 0.1501266658687186 1104 10-11 -0.5527556878739901 1104 12-13 -0.811157607549756 1104 14-15 0.3438809819570068 1104 16-17 -0.4911608181131193 1104 18-19 -0.07671961686466489 1104 20-21 0.46776016533510756 1104 22-23 -0.2402345326281008 1104 24-25 0.02352716626123197 1104 26-27 -0.2999659125373668 1104 28-29 -0.1454717180832361 1104 30-31 -0.4609696079588588 1104 32-33 -0.788611487468831 1104 34-35 -0.3996013769253537 1104 36-37 -0.40412703809863615 1104 38-39 0.12962992012354135 1104 40-41 -0.47164912065565545 1104 42-43 -0.18487818011052326 1104 44-45 -2.5143096731927734 1104 46-47 -1.3108077692280276 1104 48-49 -0.04294355524196547 1104 50-51 0.512401873865894 1104 52-53 0.4541838641327871 1104 54-55 0.23868491996045904 1104 56-57 0.2165498703303399 1104 58-59 0.272590723461704 1104 60-61 -0.05915636898183507 1104 62-63 -0.08164122319412215 1104 64-65 -0.3157839070227304 1104 66-67 -0.3312866291395977 1104 68-69 -3.15581137957993 1104 70-71 -0.8048420136699441 1104 72-73 -0.07495333422596318 1104 74-75 -0.2647355233604003 1104 76 -0.8590300177901717 1105 1 -0.06711050591444945 1105 2 -0.8391360727051982 1105 3 -1.1289059112083137 1105 4 -0.667485097187619 1105 5 0.22062472360845575 1105 6 -0.3693455364698828 1105 7 0.23062469702577104 1105 8 0.4969815370468105 1105 9 -0.08536484617233953 1105 10-11 -0.47490827374054945 1105 12-13 -1.0412454480550863 1105 14-15 -0.8602494957374276 1105 16-17 -0.5349576992816942 1105 18-19 -0.7077894944801386 1105 20-21 -0.7408610348228066 1105 22-23 -1.0788152669352478 1105 24-25 -0.8574193398579943 1105 26-27 -0.6227550953245746 1105 28-29 -1.22156725696993 1105 30-31 -0.9279553955624884 1105 32-33 -0.8323096714404059 1105 34-35 -0.7742312624366079 1105 36-37 -0.07931456277293591 1105 38-39 -0.3351598548468502 1105 40-41 0.24617559312247295 1105 42-43 -0.30553550344254177 1105 44-45 0.40577027153679524 1105 46-47 -0.68548700333778 1105 48-49 -0.050123776323690805 1105 50-51 -0.39126966186249135 1105 52-53 -1.2041511141538308 1105 54-55 -0.7063654156100121 1105 56-57 -0.9471887794920022 1105 58-59 -0.8712110925667211 1105 60-61 -0.06626256716580414 1105 62-63 -0.24002555007924187 1105 64-65 -0.6151818541210332 1105 66-67 -0.5927108296923009 1105 68-69 -0.3964351458649702 1105 70-71 0.003636075552321927 1105 72-73 -0.3139732710597549 1105 74-75 -0.42721578392099957 1105 76 -0.5667382688758416 1106 1 0.01798415500912043 1106 2 -0.3033251942117623 1106 3 -0.07651684980392659 1106 4 -5.513097489746599E-4 1106 5 0.0362112206534313 1106 6 -0.2730318031231924 1106 7 0.9505986527505037 1106 8 0.36565828752262064 1106 9 -0.5460980926914196 1106 10-11 0.35269865804347944 1106 12-13 0.2253327348558649 1106 14-15 0.1233476193582348 1106 16-17 -0.6398109498075861 1106 18-19 0.33548407058046337 1106 20-21 0.9644129309189537 1106 22-23 -0.10338203043319183 1106 24-25 0.4934020565158441 1106 26-27 0.032233385092133915 1106 28-29 -0.4536367751508372 1106 30-31 -0.5027826018131201 1106 32-33 0.7372335520395126 1106 34-35 0.2603525300105538 1106 36-37 -0.18902607233919966 1106 38-39 0.8322747840392566 1106 40-41 -0.2712540372491574 1106 42-43 -0.307463781515267 1106 44-45 0.6583746990635895 1106 46-47 0.7395104924049853 1106 48-49 -0.17030308114187775 1106 50-51 -0.02359461428160259 1106 52-53 -0.24835125439223305 1106 54-55 -0.12232254272610632 1106 56-57 0.2999563672574723 1106 58-59 0.04058896753545227 1106 60-61 0.4928629462069303 1106 62-63 -0.5281403452309981 1106 64-65 -0.3744976910437998 1106 66-67 -0.17462508392450005 1106 68-69 0.6563045115526407 1106 70-71 -0.6963038222739861 1106 72-73 0.8152464023851032 1106 74-75 -0.5572618622541654 1106 76 -1.1288939335057115 1107 1 -1.073956143498343 1107 2 -0.759046587246587 1107 3 -0.9835815264208421 1107 4 -1.498760264972855 1107 5 -0.9452813166600009 1107 6 -0.889748221033642 1107 7 -1.394475974115167 1107 8 -0.8498641005370828 1107 9 -0.9894811772685337 1107 10-11 -0.6648635310112425 1107 12-13 -0.03934388205955841 1107 14-15 -1.017050390592015 1107 16-17 -0.7182686612503737 1107 18-19 -0.25436667568819615 1107 20-21 -0.18464179544920256 1107 22-23 -0.9793521796869236 1107 24-25 -1.501423814130927 1107 26-27 -0.6273188537138381 1107 28-29 -1.4817462278871574 1107 30-31 -1.5394990197235643 1107 32-33 -1.0702291345276507 1107 34-35 -0.393179808297905 1107 36-37 -0.9825584106476555 1107 38-39 -0.2209092955627341 1107 40-41 -0.7414530422242827 1107 42-43 -0.9816428859928763 1107 44-45 -1.3808292810359113 1107 46-47 -0.7987979653064592 1107 48-49 -0.5931886532811816 1107 50-51 -0.9225000869184186 1107 52-53 -0.5459631946907422 1107 54-55 -0.14381508003954124 1107 56-57 -0.3816854237873031 1107 58-59 -0.4199582961461381 1107 60-61 -1.6077838199622256 1107 62-63 -1.1928667133902024 1107 64-65 -0.5786270442776313 1107 66-67 -1.3142768252180304 1107 68-69 -0.9342427521289522 1107 70-71 -0.5235675038660226 1107 72-73 -0.7050023538338017 1107 74-75 -0.9988041508113774 1107 76 -1.6381476648489937 1108 1 -0.8670057888884131 1108 2 0.0466271716186597 1108 3 0.2442198920188723 1108 4 0.3149986002753735 1108 5 0.3843640734109215 1108 6 -0.29499644798399416 1108 7 0.6144601960975997 1108 8 1.3070153320870297 1108 9 -0.553878340389101 1108 10-11 0.28357618530081297 1108 12-13 0.3361880328340554 1108 14-15 0.19408436118103367 1108 16-17 -0.26564454777519586 1108 18-19 -0.4709624203690481 1108 20-21 0.379968133628811 1108 22-23 0.08178257208612649 1108 24-25 -0.011281970159291888 1108 26-27 0.19622724557694227 1108 28-29 0.6061970345241932 1108 30-31 0.8334087816948745 1108 32-33 0.9478559718553257 1108 34-35 0.02178473070918585 1108 36-37 0.8312713765863862 1108 38-39 1.1002963781961306 1108 40-41 0.5345044045842293 1108 42-43 0.35282519911350363 1108 44-45 0.9122912863399755 1108 46-47 0.9065211836297102 1108 48-49 0.8447546091301668 1108 50-51 0.6511169343581784 1108 52-53 0.020490706018478022 1108 54-55 0.9137735724427287 1108 56-57 0.8534918811772343 1108 58-59 -0.34286609756457764 1108 60-61 0.7303722084065676 1108 62-63 0.40472193909206666 1108 64-65 0.22264955146705034 1108 66-67 -0.5261917188350509 1108 68-69 0.5068919996440968 1108 70-71 2.0330282408148257 1108 72-73 1.1309550929747054 1108 74-75 1.3132525867772742 1108 76 0.7998665195481678 1109 1 -0.5905078676362727 1109 2 -0.21881670218911609 1109 3 0.08354491036076794 1109 4 0.7308949074409377 1109 5 0.41058075230551694 1109 6 0.27370005482842785 1109 7 -0.7758552844599969 1109 8 0.4839290029111929 1109 9 1.152357903191234 1109 10-11 0.7593893425519767 1109 12-13 -0.25601054872622697 1109 14-15 -0.40796993082189914 1109 16-17 -0.10056751182508705 1109 18-19 0.2883919450014609 1109 20-21 0.6885765953553928 1109 22-23 0.26478575134755644 1109 24-25 0.27639227782309916 1109 26-27 0.22026735318271307 1109 28-29 0.09986296751514345 1109 30-31 0.11291477337988098 1109 32-33 0.6452881068516589 1109 34-35 0.2731420307825587 1109 36-37 0.6720392905017682 1109 38-39 0.26794127914990895 1109 40-41 0.09153546352284181 1109 42-43 0.6059433209036769 1109 44-45 -0.6462315798864857 1109 46-47 0.2730411151533083 1109 48-49 -0.25468290615474487 1109 50-51 0.031063131472386374 1109 52-53 0.7343816328954631 1109 54-55 0.03480560961563128 1109 56-57 0.35072836931614404 1109 58-59 0.047742853279149955 1109 60-61 0.771066754750418 1109 62-63 -0.13861383982698428 1109 64-65 0.2990741051476533 1109 66-67 0.5689415655865666 1109 68-69 0.25656184557219675 1109 70-71 0.24034054211098876 1109 72-73 0.16959534731562087 1109 74-75 1.2965981480391946 1109 76 0.5011626799785915 1110 1 -0.7770547350476384 1110 2 -0.40317804264564927 1110 3 -0.14714960153821366 1110 4 -0.8094644903249666 1110 5 -0.3413376546881679 1110 6 0.6899700888255147 1110 7 1.6838338850397605 1110 8 2.1743612515755935 1110 9 -0.47746239792581235 1110 10-11 0.04940876945823902 1110 12-13 0.011595085076589129 1110 14-15 -0.12334147040422039 1110 16-17 -0.2569541072597623 1110 18-19 -0.13450752075861772 1110 20-21 -0.5614962555431013 1110 22-23 -0.05019725010945919 1110 24-25 1.2919095192024095 1110 26-27 -0.7052061776575016 1110 28-29 -0.39630021849748687 1110 30-31 0.5834587267553069 1110 32-33 0.16286945702164246 1110 34-35 -0.34186525430729375 1110 36-37 0.08551670672323297 1110 38-39 0.30594516453116327 1110 40-41 0.5314577558977831 1110 42-43 0.1199064097817697 1110 44-45 1.6799218926730113 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30-31 0.7135168532923082 2116 32-33 0.3924692781707577 2116 34-35 0.14539162027352148 2116 36-37 0.6087114306221828 2116 38-39 0.7111541965007575 2116 40-41 0.2169596561884184 2116 42-43 0.4608967965468054 2116 44-45 1.4515516713450403 2116 46-47 0.8813607648522712 2116 48-49 0.3710970610045301 2116 50-51 0.3090872146688852 2116 52-53 0.7780050592775112 2116 54-55 0.8230103167858545 2116 56-57 0.14021933811745413 2116 58-59 -0.20710115328899903 2116 60-61 -0.9769901691685767 2116 62-63 1.1711015405780536 2116 64-65 0.7966110509604647 2116 66-67 0.6088977779565745 2116 68-69 0.6546461367599363 2116 70-71 -1.6959484562469775 2116 72-73 0.21023574140429346 2116 74-75 0.6083387063314802 2116 76 0.2377253510240216 2117 1 1.154105872005534 2117 2 1.313821629807677 2117 3 0.7007595588504749 2117 4 1.2071312078953618 2117 5 0.210687675588062 2117 6 1.314437825477988 2117 7 -0.8678093074484998 2117 8 -1.560871852475067 2117 9 0.475480063041541 2117 10-11 1.2062217403065851 2117 12-13 1.2905010791807499 2117 14-15 0.844422477625038 2117 16-17 1.7358398658814096 2117 18-19 1.8792767351645168 2117 20-21 1.1261333983492463 2117 22-23 1.077081928840208 2117 24-25 0.6194288990473709 2117 26-27 0.6035338594644557 2117 28-29 1.7632150124229184 2117 30-31 0.9451521430671335 2117 32-33 1.7433367569452152 2117 34-35 0.6315488738726387 2117 36-37 0.616278798654669 2117 38-39 0.7675403168403676 2117 40-41 0.9352136244423868 2117 42-43 -0.24489869994636848 2117 44-45 1.1755273081113735 2117 46-47 1.0271710269416054 2117 48-49 0.37386561028470666 2117 50-51 1.8001355719963108 2117 52-53 1.6868275029836752 2117 54-55 0.8854872455418565 2117 56-57 0.839554886290216 2117 58-59 1.1151579829236304 2117 60-61 1.434231683913744 2117 62-63 1.3996914261446811 2117 64-65 1.3137760564975594 2117 66-67 1.7227774383478653 2117 68-69 2.2037417439950815 2117 70-71 1.9502309457463802 2117 72-73 1.3572457081817006 2117 74-75 0.4624361592661401 2117 76 1.0629376064688358 2118 1 1.1333165837743842 2118 2 1.2424685642685631 2118 3 0.5182366553973381 2118 4 1.3003306441180555 2118 5 1.1680520166733324 2118 6 1.7293426880572653 2118 7 1.942796753157559 2118 8 -1.4086519793249614 2118 9 1.0850642772769206 2118 10-11 0.9711970750493606 2118 12-13 1.2970197543040776 2118 14-15 0.7332526397110151 2118 16-17 1.1918828539011415 2118 18-19 0.27911817279665385 2118 20-21 -0.5541872499946585 2118 22-23 0.7004963051615611 2118 24-25 1.6214549737478627 2118 26-27 1.8176811462861622 2118 28-29 1.247799226658298 2118 30-31 1.5601979499734036 2118 32-33 1.426740562442042 2118 34-35 1.3115171613990668 2118 36-37 0.7409264378371923 2118 38-39 1.6206058559524195 2118 40-41 0.76475907898784 2118 42-43 1.213963174613184 2118 44-45 2.0332616280549978 2118 46-47 0.5602929437844502 2118 48-49 0.6151446800521505 2118 50-51 1.3824999130815812 2118 52-53 0.039188320460773696 2118 54-55 0.9034576472331857 2118 56-57 1.4048297277278472 2118 58-59 2.7674659462781044 2118 60-61 0.9025192103408024 2118 62-63 1.0572848017613161 2118 64-65 0.5991002284496396 2118 66-67 0.44511711417591115 2118 68-69 2.3301511872649883 2118 70-71 0.7564324961339786 2118 72-73 -0.05924477807623063 2118 74-75 1.198165546158318 2118 76 1.4945796078782791 2119 1 1.0198649947130392 2119 2 1.1986769899891847 2119 3 0.7510526199206211 2119 4 1.3973372960027568 2119 5 0.47293006545382 2119 6 1.6027721041696097 2119 7 -1.0466710960663868 2119 8 0.8441196393003167 2119 9 0.931331830861545 2119 10-11 0.8732665502895713 2119 12-13 1.597647987859137 2119 14-15 1.2456325362372525 2119 16-17 0.7041703631398697 2119 18-19 0.7975032430109934 2119 20-21 1.0538134891036393 2119 22-23 1.0154445682805573 2119 24-25 1.464673746844685 2119 26-27 0.6276404958796604 2119 28-29 0.690611495690078 2119 30-31 0.8405009802764347 2119 32-33 1.11391720693576 2119 34-35 1.391616939669575 2119 36-37 0.3528074430108816 2119 38-39 0.5829118426486488 2119 40-41 0.7522867951740899 2119 42-43 0.10356036603964469 2119 44-45 0.22478047506164955 2119 46-47 0.7772182948561444 2119 48-49 0.7543723223285745 2119 50-51 1.1436787748701995 2119 52-53 0.12972786221982702 2119 54-55 -0.23844922638100385 2119 56-57 1.2441682347492815 2119 58-59 1.093131134748166 2119 60-61 0.9616470743467183 2119 62-63 1.1102227175041044 2119 64-65 0.855844500437815 2119 66-67 0.4284874024242491 2119 68-69 0.49339952429381384 2119 70-71 -0.4386894550855338 2119 72-73 -1.1878478822890877 2119 74-75 0.37517958902601833 2119 76 1.461689733524988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 4.0 12 34.0 13 270.0 14 758.0 15 1457.0 16 1920.0 17 2357.0 18 2469.0 19 2401.0 20 2123.0 21 1694.0 22 1263.0 23 914.0 24 723.0 25 647.0 26 637.0 27 631.0 28 684.0 29 791.0 30 975.0 31 1128.0 32 1533.0 33 1923.0 34 2722.0 35 4083.0 36 7103.0 37 9158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.248790822240878 23.12627413358916 18.637326617899827 29.987608426270135 2 19.752400603126734 21.89508769145306 34.09253233870328 24.259979366716927 3 13.090230934052851 25.452345051980004 35.2809300849139 26.17649392905325 4 15.80628521545909 20.69875406713753 34.921831600666614 28.573129116736766 5 9.405999523847314 42.577970002380766 30.493611618125545 17.522418855646375 6 18.970716609792873 36.477263709229426 16.304261566542337 28.247758114435364 7 18.597730338861997 32.031188000952305 18.591778430283313 30.77930322990239 8 15.947147051821284 41.018569954765496 16.80422188715181 26.23006110626141 9 18.53027537497024 22.746210618204906 18.266407427981907 40.45710657884295 10-11 18.396357431949845 27.670422982302995 26.0812633917943 27.85195619395286 12-13 17.689072295849538 25.318427108959607 25.411673676692324 31.580826918498534 14-15 18.020395206729624 27.536505039282595 22.28096976430442 32.16212998968336 16-17 20.3009681771288 29.104832949765893 23.230299182604554 27.363899690500755 18-19 18.963772716451075 27.503769542099832 23.191611776843107 30.340845964605982 20-21 17.792238711213397 27.954130624553606 23.44059995238473 30.813030711848267 22-23 21.25624950400762 27.941234822633128 22.416871676851045 28.385643996508215 24-25 19.00742004602809 30.95389254821046 22.043885405920165 27.994801999841286 26-27 17.3686612173637 31.04317117689072 24.278827077216096 27.30934052852948 28-29 19.194905166256646 28.616776446313786 26.791524482183952 25.39679390524562 30-31 19.332791048329497 28.17633521149115 26.54452027616856 25.94635346401079 32-33 17.104793270375367 31.199904769462737 25.443417189111976 26.251884771049916 34-35 18.264423458455678 29.78235854297278 24.884929767478773 27.06828823109277 36-37 18.717760495198792 27.98289818268391 25.878898500119043 27.420442821998254 38-39 18.46778033489406 26.835171811760972 27.767637489088166 26.929410364256807 40-41 17.233751289580194 26.540552337116104 27.425402745813827 28.800293627489882 42-43 15.649551622887072 26.317355765415446 28.4342512499008 29.598841361796683 44-45 15.542417268470757 27.425402745813827 28.829061185620187 28.20311880009523 46-47 16.485794778192204 28.17831918101738 27.305372589477027 28.03051345131339 48-49 15.913419569875407 29.135584477422427 25.82632330767399 29.12467264502817 50-51 16.131656217760497 28.322156971668917 25.090270613443376 30.455916197127213 52-53 15.71998254106817 28.1654233790969 25.726132846599477 30.388461233235457 54-55 15.366835965399572 28.368780255535274 25.66462979128641 30.59975398777875 56-57 16.809181810967385 28.464010792794227 24.67264502817237 30.054162368066027 58-59 16.511586382033173 27.91345924926593 24.778787397825567 30.796166970875326 60-61 15.622768034282993 26.932386318546147 25.06646297912864 32.37838266804222 62-63 16.06916117768431 27.51468137449409 24.99702404571066 31.419133402110944 64-65 16.38362034759146 28.698119196889138 23.042814062376003 31.875446393143402 66-67 15.812237124037775 31.1334417903341 22.030989603999682 31.023331481628443 68-69 15.56820887231172 31.495516228870724 22.128204110784857 30.808070788032694 70-71 15.551345131338781 32.44881358622331 21.675859058804857 30.323982223633045 72-73 15.792397428775494 32.891238790572174 21.451670502341084 29.864693278311243 74-75 16.795294024283788 32.224625029759544 21.57566859773034 29.404412348226334 76 17.956908181890327 30.75549559558765 21.972462502975954 29.315133719546065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 1.5 11 2.0 12 2.0 13 1.5 14 2.5 15 5.0 16 8.0 17 10.0 18 14.0 19 20.5 20 24.0 21 25.0 22 29.5 23 54.5 24 82.5 25 90.0 26 120.0 27 158.5 28 208.0 29 249.0 30 322.0 31 454.0 32 600.5 33 688.0 34 793.0 35 1052.0 36 1342.5 37 1479.0 38 1596.0 39 2000.0 40 2508.5 41 2880.0 42 3030.0 43 3205.0 44 3512.5 45 3636.5 46 3628.0 47 3622.5 48 3533.0 49 3259.5 50 3070.0 51 2901.0 52 2467.5 53 2055.0 54 1907.0 55 1773.0 56 1441.0 57 1130.0 58 1017.0 59 894.5 60 684.0 61 520.0 62 444.0 63 381.0 64 278.5 65 209.0 66 154.5 67 130.0 68 119.0 69 80.0 70 48.5 71 45.0 72 46.0 73 35.5 74 21.5 75 19.0 76 16.0 77 13.5 78 10.5 79 7.0 80 5.5 81 6.0 82 5.5 83 3.0 84 2.5 85 3.5 86 4.0 87 3.0 88 2.0 89 3.5 90 4.0 91 3.5 92 5.0 93 4.5 94 5.0 95 4.5 96 3.0 97 3.5 98 6.5 99 7.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7340687247043886 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50404.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.50805491627649 #Duplication Level Percentage of deduplicated Percentage of total 1 84.78583573992606 62.32441869692882 2 7.268359828344714 10.685659868264423 3 3.5140751936519927 7.7493849694468695 4 1.8164152114652776 5.340845964605983 5 0.8393835524007449 3.08507261328466 6 0.5964751288764136 2.6307435917784305 7 0.3346738279668565 1.7220855487659708 8 0.2887911257455939 1.6982779144512339 9 0.16463793149982456 1.0891992698992143 >10 0.39135246012253383 3.674311562574399 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009919847631140385 0.0 2 0.0 0.0 0.0 0.011903817157368463 0.0 3 0.0 0.0 0.0 0.011903817157368463 0.0 4 0.0 0.0 0.0 0.011903817157368463 0.0 5 0.0 0.0 0.0 0.011903817157368463 0.0 6 0.0 0.0 0.0 0.011903817157368463 0.0 7 0.0 0.0 0.0 0.011903817157368463 0.0 8 0.0 0.0 0.0 0.011903817157368463 0.0 9 0.0 0.0 0.0 0.01388778668359654 0.0 10 0.0 0.0 0.0 0.01388778668359654 0.0 11 0.0 0.0 0.0 0.021823664788508848 0.0 12 0.0 0.0 0.0 0.021823664788508848 0.0 13 0.0 0.0 0.0 0.021823664788508848 0.0 14 0.0 0.0 0.0 0.025791603840965002 0.0 15 0.0 0.0 0.0 0.03372748194587731 0.0 16 0.0 0.0 0.0 0.03372748194587731 0.0 17 0.0 0.0 0.0 0.03967939052456154 0.0 18 0.0 0.0 0.0 0.04761526862947385 0.0 19 0.0 0.0 0.0 0.05356717720815808 0.0 20 0.0 0.0 0.0 0.05356717720815808 0.0 21 0.0 0.0 0.0 0.05555114673438616 0.0 22 0.0 0.0 0.0 0.057535116260614236 0.0 23 0.0 0.0 0.0 0.06150305531307039 0.0 24 0.0 0.0 0.0 0.06348702483929847 0.0 25 0.0 0.0 0.0 0.06348702483929847 0.0 26 0.0 0.0 0.0 0.0694389334179827 0.0 27 0.0 0.0 0.0 0.08332672010157924 0.0 28 0.0 0.0 0.0 0.10118244583763193 0.0 29 0.0 0.0 0.0 0.10316641536386001 0.0 30 0.0 0.0 0.0 0.1309419887310531 0.0 31 0.0 0.0 0.0 0.16665344020315848 0.0 32 0.0 0.0 0.0 0.2003809221490358 0.0 33 0.0 0.0 0.0 0.2440282517260535 0.0 34 0.0 0.0 0.0 0.2995793984604396 0.0 35 0.0 0.0 0.0 0.3809221490357908 0.0 36 0.0 0.0 0.0 0.4860725339258789 0.0 37 0.0 0.0 0.0 0.6785175779700023 0.0 38 0.0 0.0 0.0 0.9582572811681612 0.0 39 0.0 0.0 0.0 1.1903817157368464 0.0 40 0.0 0.0 0.0 1.5534481390365844 0.0 41 0.0 0.0 0.0 1.9105626537576383 0.0 42 0.0 0.0 0.0 2.287516863740973 0.0 43 0.0 0.0 0.0 2.652567256566939 0.0 44 0.0 0.0 0.0 3.057297039917467 0.0 45 0.0 0.0 0.0 3.521545909054837 0.0 46 0.0 0.0 0.0 3.934211570510277 0.0 47 0.0 0.0 0.0 4.309181810967384 0.0 48 0.0 0.0 0.0 4.755574954368701 0.0 49 0.0 0.0 0.0 5.211887945401159 0.0 50 0.0 0.0 0.0 5.580906277279581 0.0 51 0.0 0.0 0.0 5.991587969208793 0.0 52 0.0 0.0 0.0 6.406237600190461 0.0 53 0.0 0.0 0.0 6.83080707880327 0.0 54 0.0 0.0 0.0 7.185937623998095 0.0 55 0.0 0.0 0.0 7.554955955876518 0.0 56 0.0 0.0 0.0 7.957701769700817 0.0 57 0.0 0.0 0.0 8.302912467264504 0.0 58 0.0 0.0 0.0 8.650107134354416 0.0 59 0.0 0.0 0.0 8.939766685183715 0.0 60 0.0 0.0 0.0 9.294897230378542 0.0 61 0.0 0.0 0.0 9.713514800412666 0.0 62 0.0 0.0 0.0 10.102372827553369 0.0 63 0.0 0.0 0.0 10.449567494643283 0.0 64 0.0 0.0 0.0 10.836441552257757 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGACC 15 0.0021783707 70.2749 1 ACGATGC 15 0.0022130455 69.99604 24 GCCTGAG 15 0.0022130455 69.99604 30 GCGGGGT 15 0.0022130455 69.99604 26 CGATGCA 15 0.0022130455 69.99604 25 GGCCTGA 15 0.0022130455 69.99604 29 GGGGACT 20 0.0069152745 52.497025 49 CGGGGAC 20 0.0069152745 52.497025 48 CCGTAAC 20 0.0069152745 52.497025 55 GCGGGGA 20 0.0069152745 52.497025 47 CGCAACA 20 0.0069152745 52.497025 57 CCTGAGG 20 0.0069152745 52.497025 31 GACTCAG 20 0.0069152745 52.497025 10 CAGGAGT 45 0.0042710467 31.10935 41 GCAGGAG 45 0.0042710467 31.10935 40 GAGGCCT 45 0.0042710467 31.10935 9 CCCTCGT 50 0.007148142 27.998413 52 ACCCTCG 50 0.007148142 27.998413 51 GATCTCG 65 7.8562705E-4 26.92155 41 CTTTACA 80 0.002572898 21.960907 1 >>END_MODULE