FastQCFastQC Report
Wed 25 May 2016
SRR1780416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50404
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT43158.560828505674154No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT38017.541068169192922Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC13612.700182525196413No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC7011.390762637885882No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG4740.9404015554321086No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT3560.7062931513371955Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT2170.4305213871914927No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG2110.41861757003412425No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC2050.40671375287675576No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC2010.3987778747718435No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1250.24799619077850965No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT1250.24799619077850965No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1200.23807634314736925No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT1040.20633283072772005No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC1000.1983969526228077No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT950.18847710499166734No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG900.17855725736052694No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC880.17458931830807078No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT870.17260534878184272No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC810.16070153162447426No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG740.1468137449408777No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT670.13292595825728118No Hit
ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT560.11110229346877232Illumina PCR Primer Index 1 (95% over 24bp)
CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT520.10316641536386002Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAGG150.002212548670.04
TCTATCC150.002212548670.046
CGAACCT150.002212548670.022
CGCAGGG150.002212548670.05
TACCATA207.855412E-570.064
TTTACAC2550.063.1372572
TCTTTAT453.7289283E-1062.2222251
TTACACA2650.060.754723
CTTATAA308.264906E-658.3333321
CTTTACA2850.056.4912261
TCCTCAG252.3690928E-455.99999616
CTATACA1000.055.9999961
CTTGCCC252.3690928E-455.99999647
GGCATTA200.006913725352.564
GACAGCT200.006913725352.59
TCAGGCC200.006913725352.519
GCAGGGC200.006913725352.56
AGGGCTC200.006913725352.58
CTCTCCA200.006913725352.59
CCTTGGC200.006913725352.564
Produced by FastQC (version 0.11.5)