##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50404 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.90822156971669 34.0 34.0 34.0 31.0 34.0 2 33.29819061979208 34.0 34.0 34.0 33.0 34.0 3 33.504067137528764 34.0 34.0 34.0 34.0 34.0 4 33.54987699388938 34.0 34.0 34.0 34.0 34.0 5 33.58229505594794 34.0 34.0 34.0 34.0 34.0 6 37.324259979366715 38.0 38.0 38.0 37.0 38.0 7 36.977958098563604 38.0 37.0 38.0 36.0 38.0 8 36.988254900404726 38.0 37.0 38.0 36.0 38.0 9 36.74509959527022 38.0 37.0 38.0 34.0 38.0 10-11 36.49116141576066 38.0 37.0 38.0 34.0 38.0 12-13 36.326105071026106 38.0 37.0 38.0 34.0 38.0 14-15 36.45635267042299 38.0 37.0 38.0 34.0 38.0 16-17 36.514780572970395 38.0 37.0 38.0 34.0 38.0 18-19 36.56397309737322 38.0 37.0 38.0 34.0 38.0 20-21 36.36081461788747 38.0 37.0 38.0 34.0 38.0 22-23 36.388500912625986 38.0 37.0 38.0 33.5 38.0 24-25 35.57065907467661 38.0 36.0 38.0 31.0 38.0 26-27 35.778896516149516 38.0 36.0 38.0 31.0 38.0 28-29 35.73615189270693 38.0 36.5 38.0 31.0 38.0 30-31 35.49103245774145 38.0 36.0 38.0 30.5 38.0 32-33 36.059419887310526 38.0 37.0 38.0 34.0 38.0 34-35 35.69126458217602 38.0 36.5 38.0 31.5 38.0 36-37 35.53310253154511 38.0 36.0 38.0 31.0 38.0 38-39 34.6348107293072 37.5 35.0 38.0 26.5 38.0 40-41 34.90033529084993 37.0 35.0 38.0 29.0 38.0 42-43 35.70739425442425 37.5 36.5 38.0 32.0 38.0 44-45 35.92255574954369 38.0 36.5 38.0 32.5 38.0 46-47 36.07874375049599 38.0 37.0 38.0 33.5 38.0 48-49 35.772567653360845 38.0 36.5 38.0 31.5 38.0 50-51 35.8992738671534 38.0 37.0 38.0 31.5 38.0 52-53 35.70698754067138 38.0 37.0 38.0 31.0 38.0 54-55 35.93478692167288 38.0 37.0 38.0 32.5 38.0 56-57 35.928239822236335 38.0 37.0 38.0 32.5 38.0 58-59 35.87637885882073 38.0 37.0 38.0 32.0 38.0 60-61 35.681136417744625 38.0 36.5 38.0 31.5 38.0 62-63 36.08831640346004 38.0 37.0 38.0 33.5 38.0 64-65 36.07827751765733 38.0 37.0 38.0 33.0 38.0 66-67 34.97692643440997 38.0 35.5 38.0 29.0 38.0 68-69 33.045899134989284 37.0 32.5 38.0 21.5 38.0 70-71 31.550144829775412 37.0 28.0 38.0 11.0 38.0 72-73 30.113492976747878 36.5 23.0 38.0 11.0 38.0 74-75 30.27260733275137 37.0 23.5 38.0 11.0 38.0 76 30.084497262122053 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2877947205821272 1101 2 0.5650112035847243 1101 3 0.13587699087568694 1101 4 0.15243716146355268 1101 5 0.21021852551628228 1101 6 -1.6233645855546968 1101 7 0.08447126419246587 1101 8 -0.09474734125032569 1101 9 0.05562620771446802 1101 10-11 -0.006017471732377544 1101 12-13 0.7057245221957018 1101 14-15 0.4700142709561348 1101 16-17 -0.017984755319211843 1101 18-19 -0.662409897538268 1101 20-21 -0.43506388002234075 1101 22-23 0.47589520704961075 1101 24-25 -2.5803339330337067 1101 26-27 -0.7173468999997183 1101 28-29 0.2776866372681823 1101 30-31 0.30346261023540677 1101 32-33 -0.0875783715308387 1101 34-35 0.09518315850178993 1101 36-37 0.20967523408129551 1101 38-39 -0.2543530769148745 1101 40-41 0.7053707862252665 1101 42-43 0.74003019175872 1101 44-45 0.5478150307421785 1101 46-47 0.6947143588252871 1101 48-49 -1.721230267091407 1101 50-51 -1.4805093624791255 1101 52-53 -2.1449943015189916 1101 54-55 -1.1596158651607382 1101 56-57 -1.4055456314090975 1101 58-59 -2.320271600527448 1101 60-61 -2.1508031744655938 1101 62-63 -0.8819897972703714 1101 64-65 -1.752789236433422 1101 66-67 -2.179453950936434 1101 68-69 -1.069381037325222 1101 70-71 -1.5641174599100829 1101 72-73 -2.5192166338192123 1101 74-75 -1.864193255364146 1101 76 -2.8227494174254737 1102 1 -0.5277823577068119 1102 2 -0.10205901433295139 1102 3 0.1442170500632045 1102 4 0.3130505593650881 1102 5 0.34002751125747466 1102 6 0.40286624576088315 1102 7 0.27279518132993985 1102 8 0.5154221502751 1102 9 0.4404795840932678 1102 10-11 0.999026918905237 1102 12-13 0.5631364135058945 1102 14-15 0.17542185771965535 1102 16-17 0.23026652259308378 1102 18-19 -0.18534506030394482 1102 20-21 0.7940859720088724 1102 22-23 0.4286797106815712 1102 24-25 -5.266034767039621 1102 26-27 -1.0327894612049988 1102 28-29 0.07691988989126486 1102 30-31 1.4457144262160355 1102 32-33 0.22039849153076574 1102 34-35 0.8266870594972104 1102 36-37 -2.418586805197698 1102 38-39 0.4754021019928487 1102 40-41 1.5380987926820922 1102 42-43 0.9972941142768761 1102 44-45 1.3780544711511098 1102 46-47 1.4981848995839613 1102 48-49 -3.794878370400525 1102 50-51 -0.0972701911043643 1102 52-53 -0.13732682774982408 1102 54-55 0.5533246782882841 1102 56-57 0.8071258778725863 1102 58-59 0.05193716998640241 1102 60-61 -6.391521495692388 1102 62-63 -2.4567812957906767 1102 64-65 -0.04253903465779274 1102 66-67 1.4932659844953662 1102 68-69 -1.1796177873924805 1102 70-71 -1.8678570348360992 1102 72-73 -4.554123817031478 1102 74-75 -2.288594654341818 1102 76 -0.7935592102691658 1103 1 0.9518094790278795 1103 2 0.5067845230820112 1103 3 0.31995401151445435 1103 4 0.33005736208617975 1103 5 0.29127467679035135 1103 6 0.4198730484819677 1103 7 0.8056749999240438 1103 8 0.6820888169417643 1103 9 0.31009409656492437 1103 10-11 0.6027684155038742 1103 12-13 -0.4022830876278931 1103 14-15 0.1618164155427806 1103 16-17 0.6372960010511335 1103 18-19 0.8118204725758815 1103 20-21 -0.27280745202740775 1103 22-23 0.6378633841509611 1103 24-25 -2.370343157062301 1103 26-27 -0.1711114566698484 1103 28-29 0.2588926790069124 1103 30-31 1.3351702085289645 1103 32-33 0.923346337335758 1103 34-35 0.5092267420368941 1103 36-37 -0.8789042655151533 1103 38-39 0.15000527659602625 1103 40-41 -0.15237739779410475 1103 42-43 0.09253220951497809 1103 44-45 1.0492562852100704 1103 46-47 0.9471644914207005 1103 48-49 -1.8288919758427014 1103 50-51 -0.03377812761229393 1103 52-53 0.21811534912092867 1103 54-55 -0.2607342786278153 1103 56-57 1.1438605717501318 1103 58-59 -0.38400387309749817 1103 60-61 -3.911929658957696 1103 62-63 -0.9375069193734475 1103 64-65 0.26358341432179344 1103 66-67 0.21775578041373933 1103 68-69 0.42525749605423613 1103 70-71 -0.08270964254585422 1103 72-73 -4.094940143562091 1103 74-75 -2.200726173616193 1103 76 -2.479500253353063 1104 1 0.692455737531283 1104 2 0.09541997726368834 1104 3 0.392232923079078 1104 4 -0.1561371157049365 1104 5 0.2252982862341284 1104 6 0.2383004194303453 1104 7 0.6020435473430155 1104 8 0.441892738510397 1104 9 1.161184532739398 1104 10-11 -0.18392126036646772 1104 12-13 0.7099618103312935 1104 14-15 0.9404778801286184 1104 16-17 0.4324140482700187 1104 18-19 0.8550377594906422 1104 20-21 0.052722760057420714 1104 22-23 0.03372172748829172 1104 24-25 0.7338485196083653 1104 26-27 0.8953151139584037 1104 28-29 1.4575221307876305 1104 30-31 1.0917228295773782 1104 32-33 0.6307042805130365 1104 34-35 0.34798724623858135 1104 36-37 1.5351363507313422 1104 38-39 1.6914968732346765 1104 40-41 1.743631005567245 1104 42-43 0.6338487361256213 1104 44-45 0.8520273936534437 1104 46-47 1.1765812581273778 1104 48-49 0.20704739990760146 1104 50-51 0.9305075866734143 1104 52-53 0.8323860574042428 1104 54-55 0.03610945886718042 1104 56-57 0.40726593389499044 1104 58-59 -0.7778364061107013 1104 60-61 0.9218538544476687 1104 62-63 0.41430880691707017 1104 64-65 1.0476220297679788 1104 66-67 0.6291203262320622 1104 68-69 2.1282136785272243 1104 70-71 2.2108077644169377 1104 72-73 0.2692305247052147 1104 74-75 -0.4014564657330375 1104 76 -0.2747683605959601 1105 1 0.7788651335044392 1105 2 0.4554673519182444 1105 3 0.06756849354887606 1105 4 -0.15850584843293092 1105 5 -0.7746523651673698 1105 6 0.19497234994751977 1105 7 0.3056065161546968 1105 8 -0.8100812054295972 1105 9 0.7319997715866151 1105 10-11 0.28274376134690726 1105 12-13 -0.4746019480579662 1105 14-15 0.1170965141594138 1105 16-17 -0.007775450348219692 1105 18-19 -0.022380321835839823 1105 20-21 -0.0797288783160468 1105 22-23 0.0032242936154176505 1105 24-25 0.2880826293596499 1105 26-27 0.44111061336622015 1105 28-29 0.6353251311824195 1105 30-31 0.6693481750175678 1105 32-33 0.6015639330988947 1105 34-35 -1.066516306860514 1105 36-37 0.41479979328957484 1105 38-39 -0.12899759972228964 1105 40-41 -0.34988458858988025 1105 42-43 -0.058314706432611274 1105 44-45 0.33549561315600585 1105 46-47 0.6992075905395367 1105 48-49 -0.46298319622347606 1105 50-51 0.7375545665391883 1105 52-53 0.0017751217548109821 1105 54-55 0.757117157248544 1105 56-57 0.1562789619249969 1105 58-59 -0.0913727266791966 1105 60-61 0.26971212527277544 1105 62-63 -0.7914995231263617 1105 64-65 -0.09990560544718363 1105 66-67 0.16504610593992197 1105 68-69 -0.11727092471004852 1105 70-71 0.36674933567630674 1105 72-73 -0.45281349424464423 1105 74-75 -0.562777034228894 1105 76 -0.4561244600827372 1106 1 -1.3504547102299114 1106 2 -0.1969417435440377 1106 3 0.270964266362661 1106 4 0.2007548948306166 1106 5 0.2752324389997156 1106 6 0.20954712706862466 1106 7 -1.5912180856684017 1106 8 -0.10398083479952902 1106 9 -0.8176346068567426 1106 10-11 0.04451377036223647 1106 12-13 0.8890662879432369 1106 14-15 -1.3116072822945597 1106 16-17 0.35559227478450595 1106 18-19 0.7981896471113643 1106 20-21 0.637665692454263 1106 22-23 0.7117287511932986 1106 24-25 -0.5937414716119989 1106 26-27 0.4879402237037951 1106 28-29 0.2807223063802482 1106 30-31 0.6157570701392743 1106 32-33 -0.06436815144483177 1106 34-35 0.7096531812693101 1106 36-37 1.21473468260141 1106 38-39 0.19264919983695705 1106 40-41 1.3572174849351129 1106 42-43 1.0857802550018292 1106 44-45 1.030802635093302 1106 46-47 0.7243753757744358 1106 48-49 1.0395210610138292 1106 50-51 0.2514031090614708 1106 52-53 0.3582310969125828 1106 54-55 -0.05020529091333259 1106 56-57 -0.15484995723758033 1106 58-59 0.566270266409056 1106 60-61 0.9151593856153539 1106 62-63 0.5295963398406869 1106 64-65 -0.7790351256314949 1106 66-67 0.34685518136814864 1106 68-69 1.5703229849363467 1106 70-71 0.23780513052755836 1106 72-73 0.9711071708860786 1106 74-75 -0.7630675280684223 1106 76 -0.790242460683757 1107 1 0.6141224041979498 1107 2 -0.10967806195200325 1107 3 0.06389958974574483 1107 4 0.03876484507937761 1107 5 0.13304338427334272 1107 6 0.26477100766564376 1107 7 0.5239062924410547 1107 8 0.15542215027510053 1107 9 -0.5312664476527615 1107 10-11 0.30426501414333273 1107 12-13 -0.1767048563353768 1107 14-15 0.2635170958148976 1107 16-17 0.7728062051327598 1107 18-19 0.7471946222357388 1107 20-21 0.8515462894691836 1107 22-23 0.11058447258633208 1107 24-25 0.04904459803974248 1107 26-27 0.5660994276838949 1107 28-29 1.136443699415075 1107 30-31 0.6547620452636522 1107 32-33 1.070557221689505 1107 34-35 0.17303626584642728 1107 36-37 0.7787147821038971 1107 38-39 1.0876243242150636 1107 40-41 1.123336887920189 1107 42-43 0.8577703047530676 1107 44-45 0.7440862171828613 1107 46-47 0.9577087091077701 1107 48-49 0.6813121057899494 1107 50-51 1.0571742533400794 1107 52-53 0.8041017436787499 1107 54-55 0.4891976941612981 1107 56-57 0.795697306444012 1107 58-59 0.42368320173243745 1107 60-61 0.970383266212373 1107 62-63 0.8000441010347146 1107 64-65 0.7736514415326852 1107 66-67 -0.8824483012189148 1107 68-69 -0.6964431842178769 1107 70-71 -0.9246824316614983 1107 72-73 -0.21317143607909728 1107 74-75 -1.1901819559291162 1107 76 -2.0380036547136093 1108 1 -0.16658681566020306 1108 2 0.37159853379267815 1108 3 0.11297760393014045 1108 4 0.2618854124552641 1108 5 -0.010360871045804743 1108 6 -0.5550871483627233 1108 7 -0.42460434585682094 1108 8 -0.7434430979518538 1108 9 -0.6870111285038263 1108 10-11 0.36199551059723234 1108 12-13 0.7844298954376754 1108 14-15 0.3957865993609957 1108 16-17 -0.60137819203036 1108 18-19 0.4386839839378709 1108 20-21 0.9803406157103183 1108 22-23 -0.25799708769735474 1108 24-25 0.38627864059293415 1108 26-27 -0.9453190120324138 1108 28-29 -0.8422797048402444 1108 30-31 -0.08949327388528161 1108 32-33 0.6704153777178661 1108 34-35 0.11105045024358873 1108 36-37 1.0446013069265874 1108 38-39 1.2259931185413109 1108 40-41 0.6546134836648676 1108 42-43 -0.5320878512752998 1108 44-45 0.9503273519346251 1108 46-47 1.0913966523701788 1108 48-49 1.0181915384140652 1108 50-51 0.786890565396817 1108 52-53 -0.3903663414276295 1108 54-55 -0.5549157810160139 1108 56-57 0.3066192922596187 1108 58-59 0.5003498683991054 1108 60-61 1.2569080889074087 1108 62-63 1.202455448552442 1108 64-65 0.6535095975610474 1108 66-67 0.8210268760860515 1108 68-69 1.0849043335126183 1108 70-71 -1.0643278217324195 1108 72-73 0.2736370745592005 1108 74-75 -0.9891181261418822 1108 76 0.8222800332296529 1109 1 -0.801509779710095 1109 2 0.20779320241581445 1109 3 0.30343981963080324 1109 4 0.05278783358512129 1109 5 0.1383307405951868 1109 6 -0.13522899233435481 1109 7 -0.39080635123711005 1109 8 0.305077322688895 1109 9 0.5593082649909178 1109 10-11 0.38334547391344387 1109 12-13 0.6099618103312991 1109 14-15 0.5353561762746679 1109 16-17 0.6449901131787428 1109 18-19 0.4724819785575818 1109 20-21 0.8598221515381539 1109 22-23 -0.03067989522975978 1109 24-25 -0.5572772410407154 1109 26-27 -0.11401551484483718 1109 28-29 -0.848613771849287 1109 30-31 0.602118367102733 1109 32-33 0.4988330837584627 1109 34-35 0.14567994400734108 1109 36-37 1.1054963912992903 1109 38-39 1.157394439157592 1109 40-41 -0.14252518104532896 1109 42-43 0.647540419695602 1109 44-45 0.6275344930449265 1109 46-47 0.984720203360645 1109 48-49 0.8675190023416732 1109 50-51 -0.04707862022313236 1109 52-53 -0.5319902103442402 1109 54-55 -0.24459540928697976 1109 56-57 0.21040995012217678 1109 58-59 -0.34677656838250925 1109 60-61 0.9359005075916826 1109 62-63 0.2193544458623009 1109 64-65 0.7164100622223373 1109 66-67 0.2753677907351033 1109 68-69 0.006890149115456268 1109 70-71 0.9968118212120665 1109 72-73 0.9797021271392943 1109 74-75 0.08510540039272385 1109 76 1.4206170349415643 1110 1 -1.4888822906377328 1110 2 -0.21071092580176298 1110 3 0.08061320476922162 1110 4 -0.4270567511647556 1110 5 0.2599917410808601 1110 6 -0.19438392191182174 1110 7 0.6350800013612385 1110 8 0.40978834745819626 1110 9 0.8829119561031078 1110 10-11 -0.5172373332745153 1110 12-13 0.3155956131481972 1110 14-15 0.7351133399463521 1110 16-17 0.5689094915177364 1110 18-19 -0.2516316688440696 1110 20-21 0.6080251627086284 1110 22-23 -0.5476784382117827 1110 24-25 -0.5218474207395971 1110 26-27 0.9077426201721579 1110 28-29 0.53198360551837 1110 30-31 0.3969216696768001 1110 32-33 0.7639375033796441 1110 34-35 1.1410174865036993 1110 36-37 1.4069776929020108 1110 38-39 0.41710789229018275 1110 40-41 -1.7117335346150284 1110 42-43 0.24176091508170572 1110 44-45 1.092396076337785 1110 46-47 1.009586643380068 1110 48-49 1.5932839367758689 1110 50-51 -0.26667551191813743 1110 52-53 0.5725993962609053 1110 54-55 -0.2899572354161677 1110 56-57 0.5021292313266414 1110 58-59 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44-45 0.15980050289714143 2116 46-47 -1.0216563702573112 2116 48-49 -0.27900535452751285 2116 50-51 -0.5972701911043643 2116 52-53 0.08886364844065753 2116 54-55 0.34697547193907496 2116 56-57 0.47776079850750364 2116 58-59 -2.5988564808072425 2116 60-61 -2.022473876644767 2116 62-63 0.06305997405058861 2116 64-65 -1.8123803044990652 2116 66-67 -0.2607022694728869 2116 68-69 -1.3145384223131131 2116 70-71 0.9892858223067584 2116 72-73 1.0014317385240759 2116 74-75 1.1598180440708852 2116 76 0.9762820595721067 2117 1 0.5790836445080245 2117 2 0.5751281395983909 2117 3 0.33118641145117067 2117 4 -0.358599496005894 2117 5 -0.10091010409874457 2117 6 0.33143767433230664 2117 7 0.600960556006946 2117 8 0.6200733130657952 2117 9 -0.5873904786605166 2117 10-11 -0.29526986957760215 2117 12-13 -0.6660071819167683 2117 14-15 -0.2027619739525477 2117 16-17 -1.5871937948672397 2117 18-19 -1.2683092537332499 2117 20-21 0.08053853753120421 2117 22-23 0.21577827103595126 2117 24-25 -1.1916530763788629 2117 26-27 -1.8958385568122225 2117 28-29 -0.8751842075616665 2117 30-31 0.0279403398373006 2117 32-33 -0.8633962666825923 2117 34-35 0.6958269635208367 2117 36-37 -0.9630681636325349 2117 38-39 0.49219796762591983 2117 40-41 0.5054299111760017 2117 42-43 0.24203387064454063 2117 44-45 -0.2828905270031896 2117 46-47 -1.002368810272074 2117 48-49 -0.5008584368457107 2117 50-51 0.1301199897741867 2117 52-53 1.1027063948415403 2117 54-55 0.4490426554016054 2117 56-57 0.24344924442851124 2117 58-59 -0.5151540075698904 2117 60-61 -0.7071361136325862 2117 62-63 -0.4281729532288523 2117 64-65 -1.2252632871494882 2117 66-67 -0.664541324474726 2117 68-69 0.9642544902007231 2117 70-71 -0.23561266421963722 2117 72-73 1.7919448429906737 2117 74-75 1.2683451758538276 2117 76 2.1534692135034454 2118 1 -0.3492109291353813 2118 2 0.15335224107830214 2118 3 -1.0433731375269844 2118 4 0.2578557541702864 2118 5 0.08425550548546568 2118 6 0.17234676524140013 2118 7 0.8687547772895385 2118 8 0.36390699875995125 2118 9 -0.9094482658345768 2118 10-11 -0.769371349493035 2118 12-13 -0.5021594017899176 2118 14-15 0.8529110352088338 2118 16-17 -2.822042279715724 2118 18-19 -0.2414417414006209 2118 20-21 -0.4187567408338424 2118 22-23 0.887554169556033 2118 24-25 1.409650658645809 2118 26-27 -2.238597542013075 2118 28-29 -0.48855630058492494 2118 30-31 -0.23069250019089083 2118 32-33 0.056617827750109484 2118 34-35 0.4345514173615683 2118 36-37 -2.471436733047618 2118 38-39 0.6408061423968903 2118 40-41 0.012124766708062396 2118 42-43 0.975649092631862 2118 44-45 -1.9478834797868387 2118 46-47 0.27361780001685787 2118 48-49 0.2154030148808559 2118 50-51 -0.4255530193871948 2118 52-53 -1.5280194684424586 2118 54-55 -1.0065598815962815 2118 56-57 0.11069730644401687 2118 58-59 -0.10949861644938608 2118 60-61 -0.5462834004542998 2118 62-63 -0.6167740807834718 2118 64-65 0.7177423506235883 2118 66-67 0.6431577593871438 2118 68-69 -0.5991704569451528 2118 70-71 0.6330448410657752 2118 72-73 2.523798260890601 2118 74-75 1.5689089531617917 2118 76 2.155935739225786 2119 1 0.7991630546044561 2119 2 -0.8691902570739529 2119 3 0.281460565355502 2119 4 -0.17765791914826679 2119 5 -0.05931433930388863 2119 6 -0.44905013054574283 2119 7 -0.4209717563394335 2119 8 -0.18782988224522512 2119 9 -0.6034615696039793 2119 10-11 -0.02565368504366461 2119 12-13 -0.08678615714838145 2119 14-15 -0.34258046516071516 2119 16-17 -0.8595515184444693 2119 18-19 -0.12272407695124343 2119 20-21 -1.1201610276039844 2119 22-23 0.2861129278708887 2119 24-25 1.00148362242998 2119 26-27 0.7951238179278022 2119 28-29 0.16083394331752032 2119 30-31 1.0058189558327584 2119 32-33 0.37738648998217883 2119 34-35 0.14279236340740198 2119 36-37 -0.9247811528554521 2119 38-39 -1.595058602614202 2119 40-41 -0.4642240876895656 2119 42-43 -0.23905896353961253 2119 44-45 0.5102650789714787 2119 46-47 0.023155863579312097 2119 48-49 -0.6032407397385029 2119 50-51 0.8227840094376404 2119 52-53 0.3832887355486676 2119 54-55 -0.14218441965984852 2119 56-57 -1.7358474090031493 2119 58-59 -0.45899972509683096 2119 60-61 0.3622531849115589 2119 62-63 -0.2469477688839845 2119 64-65 0.38893599437820825 2119 66-67 0.3864134873989684 2119 68-69 0.13697144992845978 2119 70-71 -0.5634629194663745 2119 72-73 0.18048710050626937 2119 74-75 0.16388308472128799 2119 76 0.15630528837582247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 7.0 23 19.0 24 38.0 25 81.0 26 178.0 27 304.0 28 545.0 29 886.0 30 1372.0 31 2020.0 32 2934.0 33 4412.0 34 6465.0 35 7566.0 36 8363.0 37 15212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.30934052852948 24.129037377985874 10.814617887469248 37.7470042060154 2 13.276724069518291 14.778589000872946 52.9819061979208 18.96278073168796 3 12.35021030076978 28.398539798428697 41.27648599317514 17.97476390762638 4 8.729465915403539 27.47599396873264 35.249186572494246 28.545353543369572 5 8.330688040631696 42.7783509245298 37.92159352432346 10.969367510515038 6 17.294262360130148 41.52051424490119 15.7646218554083 25.42060153956035 7 17.41528450123006 39.7825569399254 17.226807396238396 25.57535116260614 8 15.226966113800492 38.342195063883814 24.299658757241488 22.1311800650742 9 15.899531783191811 20.246409015157525 16.04436155860646 47.8096976430442 10-11 16.541345924926592 25.298587413697327 32.54602809300849 25.61403856836759 12-13 20.95964605983652 16.45901118958813 29.19807951749861 33.38326323307674 14-15 22.611300690421395 21.905999523847314 18.58681850646774 36.89588127926355 16-17 26.06836758987382 27.624791683199746 16.974843266407426 29.331997460519005 18-19 23.03091024521863 25.733076739941275 17.73172764066344 33.50428537417665 20-21 20.742401396714545 21.75521783985398 17.483731449884928 40.01864931354655 22-23 23.433656059042935 31.520315847948577 17.649392905324973 27.396635187683515 24-25 25.836243155305134 33.698714387747 17.819022299817476 22.646020157130387 26-27 27.423418776287594 29.909332592651378 23.67371637171653 18.993532259344498 28-29 24.4772240298389 20.37239108007301 32.88727085151972 22.263114038568368 30-31 30.853702087135943 23.279898420760254 24.099277835092455 21.767121657011348 32-33 17.592849773827474 27.06531227680343 25.626934370288073 29.714903579081025 34-35 14.72799777795413 34.47444647250219 24.080430124593285 26.7171256249504 36-37 14.960122212522817 35.32060947543846 26.456233632251408 23.263034679787317 38-39 19.421077692246648 22.4138957225617 28.59098484247282 29.574041742718833 40-41 18.10768986588366 22.502182366478852 32.447821601460205 26.94230616617729 42-43 26.787556543131497 21.42587889850012 33.829656376478056 17.956908181890327 44-45 18.521347512102217 24.9504007618443 29.992659312752956 26.53559241330053 46-47 27.24287754940084 18.18605666216967 26.237004999603208 28.33406078882628 48-49 23.326521704626618 17.73371161018967 27.972978335052773 30.96678835013094 50-51 15.83802872787874 17.292278390603922 35.77394651218157 31.09574636933577 52-53 18.502499801603047 17.75652725974129 42.108761209427826 21.63221172922784 54-55 16.5135703515594 18.05114673438616 36.27886675660662 29.156416157447822 56-57 25.594198873105313 17.68609634156019 30.47773986191572 26.241964923418777 58-59 17.60971351480041 28.582056979604793 35.316641536386 18.49158796920879 60-61 20.492421236409808 40.8072772002222 22.01710181731609 16.683199746051898 62-63 13.611022934687721 50.776724069518295 18.621537973176732 16.990715022617252 64-65 13.157685897944607 52.210142052218075 17.537298627093087 17.09487342274423 66-67 13.06939925402746 51.203277517657334 17.76347115308309 17.963852075232126 68-69 13.125942385524958 48.328505674152844 18.23565590032537 20.309896039996826 70-71 13.988969129434173 44.56193952860884 19.135386080469804 22.313705261487183 72-73 14.477025632886278 39.327235933656056 20.12042695024204 26.075311483215618 74-75 14.983929846837551 38.4820649154829 20.012300611062614 26.521704626616938 76 14.75279739703198 38.945321799857155 19.60360288865963 26.698277914451236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.5 16 2.5 17 4.0 18 2.5 19 1.5 20 7.5 21 13.0 22 11.5 23 18.0 24 31.5 25 37.0 26 61.0 27 97.0 28 132.0 29 155.0 30 198.0 31 274.5 32 402.0 33 496.0 34 575.5 35 726.0 36 896.5 37 996.0 38 1182.5 39 1797.0 40 3541.5 41 5761.0 42 6664.0 43 6610.5 44 5921.0 45 4677.5 46 4070.0 47 3597.0 48 2867.0 49 2380.0 50 2150.0 51 1991.5 52 1573.5 53 1196.5 54 1079.0 55 1061.5 56 839.0 57 584.0 58 534.0 59 422.5 60 267.5 61 212.0 62 200.0 63 144.5 64 89.0 65 85.0 66 61.5 67 42.0 68 41.0 69 31.0 70 16.5 71 11.0 72 8.5 73 3.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.32259344496468 #Duplication Level Percentage of deduplicated Percentage of total 1 60.115704336104244 21.23442583921911 2 17.052347787014156 12.046662963256885 3 8.986744551786115 9.52305372589477 4 5.088744102448888 7.1899055630505515 5 3.0611098629521454 5.4063169589715105 6 1.5614468658728375 3.309261169748433 7 1.1514266456975961 2.8469962701372906 8 0.662772410694226 1.8728672327593048 9 0.5111210963828353 1.6248710419807952 >10 1.673781172770164 8.943734624236171 >50 0.056167153448663215 1.5673359257201809 >100 0.056167153448663215 4.241726847075629 >500 0.005616715344866322 1.390762637885882 >1k 0.016850146034598966 18.80207920006349 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 4315 8.560828505674154 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 3801 7.541068169192922 Illumina PCR Primer Index 1 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 1361 2.700182525196413 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 701 1.390762637885882 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 474 0.9404015554321086 No Hit TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 356 0.7062931513371955 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT 217 0.4305213871914927 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 211 0.41861757003412425 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 205 0.40671375287675576 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 201 0.3987778747718435 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 125 0.24799619077850965 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT 125 0.24799619077850965 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 120 0.23807634314736925 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT 104 0.20633283072772005 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 100 0.1983969526228077 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT 95 0.18847710499166734 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 90 0.17855725736052694 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 88 0.17458931830807078 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT 87 0.17260534878184272 No Hit TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 81 0.16070153162447426 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 74 0.1468137449408777 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT 67 0.13292595825728118 No Hit ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT 56 0.11110229346877232 Illumina PCR Primer Index 1 (95% over 24bp) CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 52 0.10316641536386002 Illumina PCR Primer Index 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009919847631140385 0.0 2 0.0 0.0 0.0 0.011903817157368463 0.0 3 0.0 0.0 0.0 0.011903817157368463 0.0 4 0.0 0.0 0.0 0.011903817157368463 0.0 5 0.0 0.0 0.0 0.011903817157368463 0.0 6 0.0 0.0 0.0 0.01388778668359654 0.0 7 0.0 0.0 0.0 0.01388778668359654 0.0 8 0.0 0.0 0.0 0.015871756209824617 0.0 9 0.0 0.0 0.0 0.017855725736052694 0.0 10 0.0 0.0 0.0 0.017855725736052694 0.0 11 0.0 0.0 0.0 0.025791603840965002 0.0 12 0.0 0.0 0.0 0.02777557336719308 0.0 13 0.0 0.0 0.0 0.02777557336719308 0.0 14 0.0 0.0 0.0 0.031743512419649234 0.0 15 0.0 0.0 0.0 0.037695420998333465 0.0 16 0.0 0.0 0.0 0.037695420998333465 0.0 17 0.0 0.0 0.0 0.04166336005078962 0.0 18 0.0 0.0 0.0 0.04761526862947385 0.0 19 0.0 0.0 0.0 0.05555114673438616 0.0 20 0.0 0.0 0.0 0.05555114673438616 0.0 21 0.0 0.0 0.0 0.057535116260614236 0.0 22 0.0 0.0 0.0 0.05951908578684231 0.0 23 0.0 0.0 0.0 0.06150305531307039 0.0 24 0.0 0.0 0.0 0.06348702483929847 0.0 25 0.0 0.0 0.0 0.06348702483929847 0.0 26 0.0 0.0 0.0 0.06745496389175462 0.0 27 0.0 0.0 0.0 0.08332672010157924 0.0 28 0.0 0.0 0.0 0.10118244583763193 0.0 29 0.0 0.0 0.0 0.10515038489008809 0.0 30 0.0 0.0 0.0 0.13689389730973733 0.0 31 0.0 0.0 0.0 0.17062137925561463 0.0 32 0.0 0.0 0.0 0.20633283072772002 0.0 33 0.0 0.0 0.0 0.2519641298309658 0.0 34 0.0 0.0 0.0 0.2995793984604396 0.0 35 0.0 0.0 0.0 0.384890088088247 0.0 36 0.0 0.0 0.0 0.49004047297833503 0.0 37 0.0 0.0 0.0 0.6924053646535989 0.0 38 0.0 0.0 0.0 0.976113006904214 0.0 39 0.0 0.0 0.0 1.1983175938417585 0.0 40 0.0 0.0 0.0 1.5653519561939528 0.0 41 0.0 0.0 0.0 1.952226013808428 0.0 42 0.0 0.0 0.0 2.3271962542655347 0.0 43 0.0 0.0 0.0 2.718038250932466 0.0 44 0.0 0.0 0.0 3.1743512419649234 0.0 45 0.0 0.0 0.0 3.6465359892072056 0.0 46 0.0 0.0 0.0 4.073089437346242 0.0 47 0.0 0.0 0.0 4.475835251170542 0.0 48 0.0 0.0 0.0 4.930164272676771 0.0 49 0.0 0.0 0.0 5.4063169589715105 0.0 50 0.0 0.0 0.0 5.7951749861122135 0.0 51 0.0 0.0 0.0 6.233632251408618 0.0 52 0.0 0.0 0.0 6.668121577652567 0.0 53 0.0 0.0 0.0 7.114514721053885 0.0 54 0.0 0.0 0.0 7.477581144353623 0.0 55 0.0 0.0 0.0 7.878342988651695 0.0 56 0.0 0.0 0.0 8.291008650107134 0.0 57 0.0 0.0 0.0 8.64613919530196 0.0 58 0.0 0.0 0.0 8.991349892865646 0.0 59 0.0 0.0 0.0 9.318704864693279 0.0 60 0.0 0.0 0.0 9.705578922307753 0.0 61 0.0 0.0 0.0 10.130148400920563 0.0 62 0.0 0.0 0.0 10.55471787953337 0.0 63 0.0 0.0 0.0 10.913816363780652 0.0 64 0.0 0.0 0.0 11.316562177604952 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCAGG 15 0.0022125486 70.0 4 TCTATCC 15 0.0022125486 70.0 46 CGAACCT 15 0.0022125486 70.0 22 CGCAGGG 15 0.0022125486 70.0 5 TACCATA 20 7.855412E-5 70.0 64 TTTACAC 255 0.0 63.137257 2 TCTTTAT 45 3.7289283E-10 62.222225 1 TTACACA 265 0.0 60.75472 3 CTTATAA 30 8.264906E-6 58.333332 1 CTTTACA 285 0.0 56.491226 1 TCCTCAG 25 2.3690928E-4 55.999996 16 CTATACA 100 0.0 55.999996 1 CTTGCCC 25 2.3690928E-4 55.999996 47 GGCATTA 20 0.0069137253 52.5 64 GACAGCT 20 0.0069137253 52.5 9 TCAGGCC 20 0.0069137253 52.5 19 GCAGGGC 20 0.0069137253 52.5 6 AGGGCTC 20 0.0069137253 52.5 8 CTCTCCA 20 0.0069137253 52.5 9 CCTTGGC 20 0.0069137253 52.5 64 >>END_MODULE